LOCUS       sequence-389537-       11908 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expressing bioluminescent pH indicator-GeNL- in Arabidopsis 
            thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Tran Q, Osabe K, Entani T, Wazawa T, Hattori M, Nagai T
  TITLE     Application of Green enhanced Nano-lantern as a bioluminescent 
            ratiometric indicator for measurement of Arabidopsis thaliana root 
            apoplastic fluid pH.
  JOURNAL   Plant Cell Environ. 2022 Jul 21. doi: 10.1111/pce.14404.
  PUBMED    35864560
REFERENCE   2  (bases 1 to 11908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Environ. 2022 Jul 21. doi: 10.1111/pce.14404."
COMMENT     Sequence Label: 35S::GeNL/pCAMBIA1301
FEATURES             Location/Qualifiers
     source          1..11908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     175..197
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     189..206
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     190..206
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        724..1069
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     5'UTR           1068..1125
                     /label=AtADH 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the 
                     Arabidopsis thaliana alcohol dehydrogenase gene (Sugio et 
                     al., 2008"
     terminator      2324..2572
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3225..3241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3225..3241)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3238..3260)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3249..3265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3273..3279,3280..3297,3298..3303))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3318..3339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3530..4206
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4273..5298
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5339..5513
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5591..5615
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6040..6834
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6921..7509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7410..7429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7663..7680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7695..7835
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7761..7780)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7921..7943
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8179..8373
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8439..9512)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9941..10570)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11871..11895
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag cattcgccat
       61 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
      121 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
      181 cacgacgttg taaaacgacg gccagtgcca agcttgcatg cctgcaggtc gactctagat
      241 tagccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg cttacgcagc
      301 aggtctcatc aagacgatct acccgagcaa taatctccag gaaatcaaat accttcccaa
      361 gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca cagagaaaga
      421 tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct tgcttcacaa
      481 accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg aatcaaaggc
      541 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
      601 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
      661 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      721 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      781 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      841 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      901 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      961 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
     1021 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaatac atcacaatca
     1081 cacaaaacta acaaaagatc aaaagcaagt tcttcactgt tgataatgca tatggtgtcc
     1141 aagggcgaag aggacaacat ggccagcctg cctgccaccc acgagctgca catcttcggc
     1201 agcatcaacg gcgtggactt cgacatggtg ggacagggca ccggcaaccc caacgacggc
     1261 tacgaggaac tgaacctgaa gtccacaaag ggcgacctgc agttcagccc ctggattctg
     1321 gtgccccaca tcggctacgg cttccaccag tacctgccct accccgacgg catgagccct
     1381 ttccaggccg ctatggtgga tggcagcggc taccaggtgc accggaccat gcagtttgag
     1441 gacggcgcca gcctgaccgt gaactaccgg tacacatacg agggcagcca catcaagggc
     1501 gaggcccaag tgaagggcac aggctttcca gccgacggcc ccgtgatgac caatagcctg
     1561 acagccgccg actggtgcag aagcaagaaa acctacccca atgacaagac catcatcagc
     1621 accttcaagt ggtcctacac caccggcaat ggcaagcggt acagaagcac cgcccggacc
     1681 acctacacct tcgccaaacc tatggccgcc aactacctga agaaccagcc tatgtacgtg
     1741 ttccgcaaga ccgagctgaa gcactccaag acagaactga acttcaaaga gtggcagaaa
     1801 gccttcaccg ggtttgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
     1861 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
     1921 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
     1981 atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag
     2041 gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta
     2101 atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc
     2161 gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc
     2221 gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg
     2281 accggctggc ggctgtgcga acgcattctg gcgtaagagc tcatatgaag atgaagatga
     2341 aatatttggt gtgtcaaata aaaagctagc ttgtgtgctt aagtttgtgt ttttttcttg
     2401 gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatctcatta
     2461 taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttcgcat
     2521 ataactactg tatgtgctat ggtatggact atggaatatg attaaagata agactagatg
     2581 ggctcataga gtaaaacgag gcgagggacc tataaacctc ccttcatcat gctatttcat
     2641 gatctatttt ataaaataaa gatgtagaaa aaagtaagcg taataaccgc aaaacaaatg
     2701 atttaaaaca tggcacataa tgaggagatt aagttcggtt tacgtttatt ttagtactaa
     2761 ttgtaacgtg agactacgta tcgggaatcg cctaattaaa gcattaatgc gaacctgatt
     2821 agattcaccg accctcctat cgtgtcgacc tttctgtttc ttagaatttt ttggtagtct
     2881 atgtactaat aatgtcagct tcgtatttat ttcataagca atttgcattt gcaatttgtt
     2941 ttttactttt atttttattg tattgtggaa tgtggactcg taccaacatg aagttatata
     3001 ccaccaaaaa aattacagtt agtcaaaaga ttcacgagtg agagctactt atgattgtct
     3061 tttacgtata tgtctaattg tctatttgct caataatctt tgtactttct tttgtcgttg
     3121 ataaaatcac aaagttccaa aagtaatcga atgatttgct tttaagaaaa gaagagctga
     3181 ataattcaac atatatctgt acactagttt gaattcgtaa tcatgtcata gctgtttcct
     3241 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3301 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3361 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3421 agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg
     3481 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
     3541 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     3601 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
     3661 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     3721 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     3781 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
     3841 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
     3901 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
     3961 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
     4021 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     4081 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     4141 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
     4201 cgctgaaatc accagtctct ctctacaaat ctatctctct cgagctttcg cagatccggg
     4261 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     4321 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     4381 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     4441 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     4501 gtgcttgaca ttggggagtt tagcgagagc ctgacctatt gcatctcccg ccgttcacag
     4561 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctaca accggtcgcg
     4621 gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     4681 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     4741 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     4801 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     4861 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     4921 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     4981 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     5041 gcaggatcgc cacgactccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     5101 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     5161 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     5221 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     5281 ccgagggcaa agaaatagag tagatgccga ccgggatctg tcgatcgaca agctcgagtt
     5341 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
     5401 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     5461 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa
     5521 ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     5581 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     5641 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     5701 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     5761 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     5821 gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga
     5881 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag
     5941 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg
     6001 tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca
     6061 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
     6121 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
     6181 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
     6241 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
     6301 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
     6361 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
     6421 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
     6481 aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat
     6541 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
     6601 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
     6661 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
     6721 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
     6781 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta
     6841 gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6901 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6961 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7021 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7081 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7141 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7201 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7261 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7321 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7381 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7441 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7501 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7561 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7621 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7681 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     7741 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     7801 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     7861 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7921 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
     7981 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
     8041 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
     8101 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
     8161 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
     8221 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
     8281 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
     8341 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
     8401 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
     8461 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
     8521 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
     8581 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
     8641 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
     8701 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
     8761 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
     8821 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
     8881 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
     8941 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
     9001 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
     9061 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
     9121 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
     9181 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
     9241 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
     9301 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
     9361 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
     9421 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
     9481 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
     9541 tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg cccgttcctc
     9601 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
     9661 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
     9721 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
     9781 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
     9841 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
     9901 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
     9961 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    10021 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    10081 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    10141 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    10201 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    10261 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    10321 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    10381 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    10441 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    10501 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    10561 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    10621 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    10681 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    10741 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    10801 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    10861 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    10921 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    10981 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    11041 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    11101 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    11161 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    11221 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    11281 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    11341 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    11401 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    11461 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    11521 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    11581 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    11641 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    11701 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    11761 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt
    11821 cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc
    11881 caatatatcc tgtcaaacac tgatagtt
//
