LOCUS       sequence-389661-       13492 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into 
            human NEAT1 locus (long isoform only). Encodes for intronic 
            IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Truong DJ, Armbrust N, Geilenkeuser J, Lederer EM, Santl TH, Beyer 
            M, Ittermann S, Steinmassl E, Dyka M, Raffl G, Phlairaharn T, 
            Greisle T, Zivanic M, Grosch M, Drukker M, Westmeyer GG
  TITLE     Intron-encoded cistronic transcripts for minimally invasive 
            monitoring of coding and non-coding RNAs.
  JOURNAL   Nat Cell Biol. 2022 Nov;24(11):1666-1676. doi: 
            10.1038/s41556-022-00998-6. Epub 2022 Nov 7.
  PUBMED    36344775
REFERENCE   2  (bases 1 to 13492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-022-00998-6"; journalName: "Nat Cell Biol"; date: 
            "2022-11"; volume: "24"; issue: "11"; pages: "1666-1676"
COMMENT     Sequence Label: pINSPECT:NEAT1-v2_SigP:NLuc
FEATURES             Location/Qualifiers
     source          1..13492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2187..2220
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2291..2324
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    2923..3044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2960..2979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3014..3033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3076..3095
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3094..3149
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    complement(3256..3289)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3360..3393)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(3442..3498)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4633..4689)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     5276..5295
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5322..6504)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5331..6273
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5354..5374)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    join(6580..7153,7154..7156,7157..7165)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6747..6764)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6974..6993
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(11304..11324)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11304..11322)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11343..11359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11343..11359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11356..11378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11367..11383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11391..11397,11398..11415,11416..11421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11436..11457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11574..11591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11745..12333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11825..11844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12504..13295,13296..13364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13127..13146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13365..13469)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgt
      661 ctcgtagcca tctcaaagtg tgctgcgggg atctcttttg catccctctg ggtgcagagc
      721 tgaggcaccc agaggcagtg tccagaggag gcagcatctg taggtgtctt cacctgctct
      781 ggctcttggc acatctggtt ggtgacactg ttttgtgaga tgggttgaaa gcacgtgctg
      841 ccaaaataga ataatgttgg tcctctcctc atgtgccgtg gaactggggt aaaactgcgt
      901 agtggctgca gctgcctgtc cataccggaa tcgagtataa cacggtgcct ggcttagcac
      961 aaaacagtag tgggtcctgc aggccccaga gtctaattcc tggtattctt tcccctacac
     1021 agattaaata aaccaaaaac aaactattct aggaaagcgt ctgtgacatt tgtaaaaagt
     1081 ggtatttaat gatcttttat tcacttgtct gtttagtttg ttgaaatctt aagtggcatc
     1141 ctggtctggg aaggagtgct gtctgcgcct gccctccgct gggcacagcg tggctgcttc
     1201 aggggctaag cacacacttt ctgtcttcta aagggctgcc acatgccagg agctcaggtg
     1261 tgagcccggc tctggctctt acctcatagg gtcactcata ggggcacagg gagcagaaca
     1321 ttgtacacag cgaggcacca cccggcttgg catctgcctc ggtggactta ctacctctag
     1381 aaggaaatac ctgagttcct ctggcctcag ctcctagagt gactggtgtg ctgtccctgt
     1441 tactcttctg tcaaggtgac aactgtgtga cccatcatct gtgtgtcaaa gcaaggccct
     1501 gcctgggcct ctgctcctgt gctgacccca aaggcaaatg ctttgctagt ttccttccag
     1561 ttaatttcac ctatgaatag atgtgtgaaa actgttcaaa gccatacctg cacatgtttg
     1621 aacttcaaac cctgtgggtg attcagtggc atctttctct aacccccagc ctcccttccc
     1681 acagaggcca ccgtcatggc cagttgctgc agtttctttc cagagaacct gtgtatgtgt
     1741 aaagctgtac aggcgtgggt acaccacaca gcctgtcttg cactgtggac tgttgagtta
     1801 ctagtacatc taggtaagca ccgcatatct gtattcatgt ctgccttggt cttttcaaca
     1861 tctgtgtggt agccgtgttt gaattaccca ttcccttttt ggggaaccat taagttgttt
     1921 cagcaatttt tactgtagat aaggctatac cgcatatctg tgtacatggg tttttatgta
     1981 catgggcaag tatatctgtg agagaaaagt ttcctcagga ggaattctgg gcacagcatg
     2041 tgtaaatttc taaatatgat ggacaccccc agcttccacc tcaaggaggt aagtatcctt
     2101 tttacagcac aacttaatga gacagataga aactggtctt gtagaaacag agtagatttt
     2161 atcgaattgc aacctcttcg agggacataa cttcgtataa tgtatgctat acgaagttat
     2221 gggtcgatgc tgagatctgg actagagggt cgatggtgat gcttggatca aaataggaag
     2281 aatcccaatg ataacttcgt ataaagtatc ctatacgaag ttatactaga attgcgtacc
     2341 gccaccatgt cgccaaggcc acctcccctg tgagctagac tggacagcca atgacgggta
     2401 agagagtgac atttctcact aacctaagac aggagggccg tcaaagctac tgcctaatcc
     2461 aatgacgggt aatagtgaca agaaatgtat cactccaacc taagacaggc gcagcctccg
     2521 agggatgtgt cttttgtttt ttataattaa aaagggtgac acgtgaggcc acctcccctg
     2581 tgagctagac tggacagcca atgacgggta agagagtgac atttctcact aacctaagac
     2641 aggagggccg tcaaagctac tgcctaatcc aatgacgggt aatagtgaca agaaatgtat
     2701 cactccaacc taagacaggc gcagcctccg agggatgtgt cttttgtttt ttataattaa
     2761 aaagggtgaa gccgacacac aaaaaaccaa cacacagatc tctagagctc tggatctttt
     2821 attgcccggg ctaccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     2881 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     2941 tataatggtt acaaatatag caatagcatc acaaatttca caaatatagc atttttttca
     3001 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtatactt
     3061 taaatgattt gccctcccat atgtccttcc gagtgagaga cacaaaaaat tccaacacac
     3121 tattgcaatg aaaatacatt tcctttatta gccagaagtc agatgccagc acaacaacca
     3181 gcacgttgcc caggagctgt aggaaaaaga agaaggcatg aacatagtta gcagagttac
     3241 gtagcgatcg ctagtataac ttcgtatagc atacattata cgaagttatg ggtcgatggt
     3301 gacaatagcg aaaaagaaag aacaatcaag ggtccccaaa ctcacctagg tcctttgata
     3361 taacttcgta taggatactt tatacgaagt tatggcatct ccttcggaag ttcctattct
     3421 atttgaagta taggaacttc caggtccagg attttcctcg acgtcccctg cctgcttgag
     3481 cagtgaaaag ttagttgccc cggatccgtt ggcttcgccc atctctctag cgaaggttct
     3541 ggcgaggtca cagatggtgg ggatgctgcc aggggttgtc acatgggtct gcaccatgcc
     3601 gcttgtcagt tgcagcaggg catctctgca tccggctggg ctctgatcgt agtccaggat
     3661 gaacacatgc atgggccgca gtctttttgc cagcacgtcg agagcccagg cgaacacgtt
     3721 atacaggtcg ccgttagggg ccagcagttc aggggctctg aacagtgtga acagggtgtc
     3781 gccgatgtga ggtctgggtc cagcgttaga ctgaggctca gcgccttgtg gaggaactgc
     3841 tgtgccagag agctgtcccc agtcctctct ccaggaaccg ccgccttgaa gatagcgcac
     3901 ggtgttagcc agcaggccgt acactcttct gatggcggcc agcatagcca gatccagtct
     3961 ctcgccaggc ctctgtctct tagccagtct gtcgatgtgt ctgtcctctg gcagggctcc
     4021 aagcacgatg tttgtgccgg gcagtgtagg aggaatcaga gccacgaaag ccagcacggc
     4081 ctgaggtgtc atgctgccca tcagataccg agcggcaggg taacacagca gggcgaagat
     4141 ggggtgtctg tcgaagatca gggtcagagc aggtggtgga gcgtgagaag atccagcctc
     4201 gccgccaata tgtggagcca gcacagcgtc tgtcacggcg taaggcatgc ccattgtgat
     4261 ctgggcagat gtcatgacca cagcagcgtc gcctgcgcta atttcgccct ggtccagtct
     4321 gtgctgtgtg gtgtagatgt tggcgattgt ctcagaggct cccaggacct gccagtaggt
     4381 cataggctcg ggcacgtaca cgatgtcatc tctgctgccc agagccacca gcagctgtgt
     4441 tgttgtggtc ttgcccatgc catgagggcc gtcgatatac actctcagca gtgtgggcat
     4501 cttctgttcc agccgcactt ctgtagcctc ttgctgcctt cttggcctga gggctgttct
     4561 tctgttgctg tggcctctgc ttctagcggc ttgatcgaag gcgctggcgt gctggtgaca
     4621 aggataagaa gcaggaccgg ggttctcttc cacgtcgcca gcctgtttca gcaggctgaa
     4681 gtttgtagcg ccggatccgg cgccaggctt tctggtcatg caccaggttc tagggccctc
     4741 aggcacttcc acgtcggcgg tcacggtgaa gcccagtctc tcgtagaagg gcaggtttct
     4801 gggggcgctt gtttccagga aggcgggcac gccagccctt tcagcagctt ccaccccagg
     4861 cagcaccaca gcagatccca gtcccttgcc ctggtggtca ggtgacacgc ccacggtggc
     4921 cagaaaccag gcaggctctt ttggtctgtg gggggccagc aggccttcca tctgctgctg
     4981 ggcagccagt ctagagccgc tcagctcggc cattctaggt ccgatctcgg cgaacacagc
     5041 gccggcttcc acagactcag gggttgtcca cacagccaca gcggcgccat catcggccac
     5101 ccacactttg ccgatgtcca ggcccactct ggtcagaaac agttcctgca gctcggtcac
     5161 tctctcgatg tgccggtcgg ggtccacggt gtgtcttgtg gcagggtaat cggcgaaggc
     5221 agcggccagt gtccgcacag ctcttggcac atcgtccctg gtggccagcc gcactgtggg
     5281 cttgtactcg gtcatggtgg cgcgcctttt aggggtagtt ttcacgacac ctgaaatgga
     5341 agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa
     5401 agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc
     5461 cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa agacgacgta
     5521 ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg cccggtactc cgtggagtca
     5581 catgaagcga cggctgagga cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc
     5641 cgctctcccg agcgccgcgt cctccatttt gagctccctg cagcagggcc gggaagcggc
     5701 catctttccg ctcacgcaac tggtgccgac cgggccagcc ttgccgccca gggcggggcg
     5761 atacacggcg gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga ctacccccgt
     5821 ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca
     5881 cgggccccgt cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag atcttggccc
     5941 gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa
     6001 atggctagag acttatcgaa agcagcgaga caggcgcgaa ggtgccacca gattcgcacg
     6061 cggcggcccc agcgcccagg ccaggcctca actcaagcac gaggcgaagg ggctccttaa
     6121 gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa gctcgggatc aagaatcacg
     6181 tactgcagcc aggggcgtgg aagtaattca aggcacgcaa gggccataac ccgtaaagag
     6241 gccaggcccg cgggaaccac acacggcact tacctgtgtt ctggcggcaa acccgttgcg
     6301 aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac cctcgggaaa
     6361 aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc ttctctaggc
     6421 accggttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg atgtgcgctc
     6481 tgcccactga cgggcaccgg agccaattcg aagttcctat tctatttgaa gtataggaac
     6541 ttcaaataca agggcttact gtgcggactg ggacgccccc ccctctccct cccccccccc
     6601 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     6661 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     6721 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     6781 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     6841 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     6901 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     6961 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     7021 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     7081 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     7141 acacgatgat aatatggcca ccaccatggt ggtcaaggtg ctgttcgccc tgatctgtat
     7201 tgccgtggcc gaggccaagc ctaccgtgtt caccctggaa gatttcgtcg gcgattggag
     7261 acagaccgcc ggatacaacc tggaccaggt gttggagcaa ggcggcgtgt ccagcctgtt
     7321 tcagaacctg ggagtgtccg tgacacccat ccagagaatc gtgctgagcg gcgagaacgg
     7381 cctgaagatc gacatccatg tgatcatccc ttacgagggc ctgtccggcg atcagatggg
     7441 ccagatcgag aagattttca aggtggtgta ccccgtggac gaccaccact tcaaagtgat
     7501 cctgcactac ggcaccctgg tcatcgatgg cgtgacccct aacatgatcg actacttcgg
     7561 cagaccctac gagggaatcg ccgtgttcga cggcaagaaa atcaccgtga ccggcacact
     7621 gtggaacggc aacaagatca tcgacgagcg gctgatcaac cccgatggca gcctgctgtt
     7681 cagagtgacc atcaacggcg tgacaggatg gcggctgtgc gaaagaatcc tggcctaatg
     7741 tacagatatc actatcgata ccgcggggat cctctagagt agaccacctc ccctgcgagc
     7801 taagctggac agccaatgac gactaagaga gtgacatttt tcactaagct aagacaggag
     7861 ggccgtcaga gctactgcct aatccaaaga cggctaaaag tgataaaaat gtatcactcc
     7921 aagttaagac aggcgcagct tccgagggat ttgcctggcc ggattgactg acgtcttaac
     7981 cactgcatat cgataccgcg gggatcctct agagtagacc acctcccctg cgagctaagc
     8041 tggacagcca atgacgacta agagagtgac atttttcact aagctaagac aggagggccg
     8101 tcagagctac tgcctaatcc aaagacggct aaaagtgata aaaatgtatc actccaagtt
     8161 aagacaggcg cagcttccga gggatttgcg tttaaacgcg gccgctctat actcgacgat
     8221 tgactatctg acaaatcata gagaacatag agaacctcct cgagtcgcct gcttttctgc
     8281 ctggtactaa cttctctccc ctctcctctt ttctttttct gcaggttggt cccattgaca
     8341 tttccccaca ccttcaccca ggctgtgccc ttaaacttgg ttatttgtca atgtgagaag
     8401 tggaaaatag tatttaattg tagtttggat ttgtatttct attgggttgt atacttactg
     8461 attaataata agagctcttt acatattaag gaaattaacc cttttcaaat acattcctat
     8521 ttctcactaa tctttaagtt ttattgtaat attttgctct ttagtttata tatatatgta
     8581 tatatatata catatatata tactaatttt cttttatggt tcctggattt tgtgagtagt
     8641 ttgaaaaggc taatccagct gaagattttg ttgttgttgt taaaccccat gttttctcct
     8701 aactcttttt atttttattt tggaggactc tatctagact taattttagc ataacaagtg
     8761 acagggttag ttagcctgtt gtccttacac cattttctgg ctaatacagc tattaactat
     8821 tgatctgtct attcacgtgc cagttcctaa tggttttaca tagtgtaatc tgcacttcaa
     8881 aatagcgaag ggaagcccta cctcattatt ctacttttcc agaattctcc tggctattcc
     8941 aggctgcatg tttaccttaa ccttccctgt gatgtcttca tgccgttgtc ttcttatgca
     9001 agaataaggt acgtctttcc atccactcac gtctatttaa tttgactttg cattacacag
     9061 aaagctggtc ttggtctgtc tacctcggca tctagttgtc ctcactgccc cctagccgac
     9121 cccaccccat ctgactgact accccatcac agagtacttt tatttacgtt ttgctctgcc
     9181 taatggttac ttgatactgt cacgccgaca gtgtccagtt cagtggtctt tgcagttgaa
     9241 atgctcccgt acacactgtc ttgttaaaaa tgccagtaag ttcatacaaa cccagcttgc
     9301 acccaaggtc acattcagag agcgtagggc tgggatgggt tgttttccaa gcttctgcca
     9361 ctgtgtggct agctcttccc actgggaagt tctgtgtacc cggaatgtcg gagtggagtc
     9421 ctgttctagt gtccagcacc tgaccctgtg cccaacccct caacagccta ttcctgctgt
     9481 ccacagcctg ctggaacttt ttacaaaata tgttgccatg ctggaccctg ggcactggac
     9541 ataagccccc tggcagcctt tttcatgtca cccaaagggg taattgtcct actggtggtc
     9601 tgtaagatga gttagggtga cttgctaata gacattgtaa atcttaatat ttatgtatgt
     9661 attttattat taccggtttt ccatttatga tggtaatatt gtttcttcta agaatattta
     9721 tttttccttc taaatattga gataaaattc atgcttttga aatgttctat tcagtggctt
     9781 ttagtatatt tgctatgttg tgcaaccatc gacactatcc atttctagaa ctttttcgtc
     9841 atcccaaaca gacgctctgt attcataaaa aaataacttc ctacctgtct ctccccctag
     9901 tctttggtaa cctttgttat actggtaaac tttgttgtgc tctctgtctg tgtgaatttg
     9961 cctattctag gggcctcata taagtgtaat catacagtat ttgtcttttt gggtctgtct
    10021 gatttcactt agcgggtttt cagggttcat tcatgttgca gcatataaca gtactgcgtt
    10081 cctttttctg gctgaataat attccactgt atggatagac cccattttgt ttattcacac
    10141 atcatttgga catttggatt atttctggtt tttggctatt atgaacaatg gtgctatgaa
    10201 cagttgcgta caagtttttg tgtgaacata tgttttcaat tctctcatta tatacctagg
    10261 agtagaatta ctgggtcata tggtaactgt atatttttga ggaactgcca aactattttc
    10321 ccacgtccat gcaccatttc acattcccac cagtaagtaa gagggttcca atttctgcgc
    10381 attcttgcca acactagtta ttatctgact ttctggttat aatcattcta atgagtgtga
    10441 agtagcctct ggtgtcattt ggatttgcat ttctctgatg agtgatgcta tcaagcacct
    10501 ttgctggtgc tgttggccat atgtgtatgt tccctggaga agtgtctgtg ctgagccttg
    10561 gcccactttt taattaggcg tttgtctttt tattactgag ttgtaagagt tctttatata
    10621 ttctggattc tagaccctta tcagatacat ggtttgcaaa tattttctcc cattctgtgg
    10681 gttgtgtttt cactttatcg ataatgtcct tagacatata ataaatttgt attttaaaag
    10741 tgacttgatt tggctgtgca aggtggctca cgcttgtaat cccagcactt tgggagactg
    10801 aggtgggtgg atcatatgag gaggctagga gttcgaggtc agcctggcca gcatagcgaa
    10861 aacttgtctc tactaaaaat acaaaaatta gtcaggcatg gtggtgcacg tctgtaatac
    10921 cagcttctca ggaggctgag gcacgaggat cacttgaacc caggaggagg aggttgcagt
    10981 gagctgagat catgccaggg caacagaatg agactttgtt taaaaaaaaa aaaaagtgac
    11041 ttgatttaag ggaaaaaatg actggctata ttcagtcaga tatggcaaaa agtctcaagg
    11101 tgttaatgtg aatgattaag gtcttggggg gggtgtcccc tatcagacta caggtgttta
    11161 gaggcacaga aaaaggtgca gttgggttct taatgtgaaa tgatgagaag cacaactcca
    11221 gtgtgtctct ttgtgtagaa tgtcagcaga caccccctgc tagatgtgct ggatcatggg
    11281 gagacggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
    11341 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    11401 gccgggagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    11461 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    11521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    11581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    11641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    11701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    11941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    12001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    12061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    12121 gcgaggtatg taggaggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    12181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    12241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    12421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    12481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12541 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12601 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    12661 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    12721 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12781 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    12841 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    12901 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    12961 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    13021 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    13081 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    13141 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    13201 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    13261 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    13321 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    13381 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13441 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
