LOCUS       sequence-389768-       12019 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 12019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SIGLEC1(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..12019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7669..7692
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9352..9396
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9397..9423
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(9563..9580)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9569..9680
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9798..9818)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9803..10132
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9983..10118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10045..10064
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10180..10227
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10246..10845
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10246..10265)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10742..10762
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10975..11096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11012..11031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11066..11085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(11149..11940,11941..12009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11772..11791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(12010..12019,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcctc ttggggagtt
     2761 agttctcctc aggatgtgca gggcgtgaag ggctcttgtc tgctgatccc ctgcatcttc
     2821 agcttccccg ctgacgtgga agtgcctgat ggaatcaccg ccatctggta ctacgactac
     2881 agcggccaga gacaggtggt gtctcactct gccgatccta agctggtgga agccagattc
     2941 agaggcagaa ccgagttcat gggcaacccc gagcacagag tgtgcaacct gctgctgaaa
     3001 gacctgcagc ctgaggacag cggcagctac aacttcagat tcgagatcag cgaagtgaac
     3061 cgttggagcg acgtgaaggg aaccctggtc acagtgaccg aggaacccag agtgcctaca
     3121 atcgcctctc ctgtggaact gctggaaggc accgaggtgg acttcaactg tagcacccct
     3181 tacgtgtgcc tgcaagagca agttcggctg cagtggcagg gacaagatcc tgccagaagc
     3241 gtgaccttca acagccagaa gtttgagcca acaggcgtgg gccacctgga aaccctgcat
     3301 atggctatgt cttggcagga ccacggcaga atcctgagat gccagctgag cgtggccaat
     3361 cacagagccc agagcgagat ccatctgcaa gtgaagtatg cccctaaagg cgtgaagatt
     3421 ctgctgagcc ccagcggcag aaatatcctg cctggcgagc tggtcaccct gacctgtcaa
     3481 gtgaatagca gctaccccgc cgtgtccagc atcaagtggc tgaaggatgg cgtgcggctg
     3541 cagacaaaaa ctggcgttct gcatctgcct caggccgctt ggagtgatgc tggcgtgtac
     3601 acctgtcagg ccgagaatgg cgttggctct ctggtgtctc ctcctatctc tctgcacatc
     3661 ttcatggccg aggtccaggt ttcccctgcc ggacctatcc tggaaaacca gaccgtgaca
     3721 ctcgtgtgca acacccctaa tgaggcccct agcgacctgc ggtacagctg gtataagaac
     3781 cacgtgctgc tcgaggacgc ccactctcac acactgagac tgcatctggc caccagagcc
     3841 gataccggct tctacttctg cgaggtgcag aatgtgcacg gctctgagag atctggccct
     3901 gtgtccgtgg tggtcaatca ccctccactg acacctgtgc tgaccgcctt tctggaaaca
     3961 caggccggac tcgtgggcat cctgcactgt tctgtggtgt ctgaacctct ggccacactg
     4021 gtgctgtctc acggcggaca tattctggcc agcacaagcg gcgatagcga tcacagccct
     4081 agattcagcg gcaccagcgg acctaacagc ctgcggctgg aaatccgcga cctggaagaa
     4141 acagactccg gcgagtacaa gtgcagcgcc accaattctc tgggcaacgc caccagcaca
     4201 ctggactttc atgccaatgc cgccagactg ctgatttctc ctgctgccga agtggtggaa
     4261 ggacaggctg tgacactgtc ctgtagaagc ggactgagcc ctacacctga tgccaggttt
     4321 agctggtatc tgaacggcgc cctgctgcat gaaggccctg gatcttctct tctgctgcct
     4381 gccgcttcta gcaccgatgc cggctcttat cactgtagag ccagagatgg acacagcgcc
     4441 tctggaccat cttctccagc tgtcctgaca gtgctgtacc ctcctagaca gcccaccttc
     4501 accaccagac tggatttgga tgctgctggt gctggcgctg ggagaagagg actgctgctg
     4561 tgtagagtgg acagcgatcc tcctgctaga ctgcagctgc tgcacaagga tagagtggtg
     4621 gccacatctc tgcctagcgg cggaggatgt tctacctgtg gcggctgtag ccccagaatg
     4681 aaggttacaa aggcccctaa cctgctgagg gtcgagatcc acaatcctct gctcgaagag
     4741 gaaggcctgt acctctgcga agccagcaat gccctgggca atgccagcac ctctgccacc
     4801 tttaatggac aggccaccgt gctggctatc gcccctagtc atacactgca agagggcaca
     4861 gaggccaacc tgacctgcaa tgtgtcaaga gaagccgccg gatctcccgc caacttcagc
     4921 tggtttagaa acggcgtgct gtgggcccag ggacctcttg aaacagtgac actgctgccc
     4981 gtggccagaa cagatgccgc actgtatgcc tgtcggattc tgacagaagc tggcgcccag
     5041 ctgtctacac ctgttctgct gtctgtgctg tatccacctg acagacctaa gctgtctgcc
     5101 ctgctggata tgggacaggg acacatggcc ctgttcatct gcaccgtgga tagcagacca
     5161 ctggctctgc ttgccctgtt tcatggcgaa catctgctgg ccacaagcct gggacctcag
     5221 gtgccatctc atggcagatt tcaggccaag gccgaggcca atagcctgaa gctggaagtc
     5281 cgagaactcg gcctgggcga ttctggcagc tatagatgcg aggccacaaa cgtgctgggc
     5341 agcagcaaca ccagcctgtt ctttcaagtc cgcggagcct gggtgcaagt gtctccatct
     5401 cctgaactcc aagagggcca agccgtcgtg ctgtcttgtc aagtgcatac aggcgtgcca
     5461 gagggcacca gctacagatg gtatagagat ggccagcctc tgcaagagtc caccagcgct
     5521 acactgagat tcgccgccat cacacttacc caggctggcg catatcattg ccaggctcaa
     5581 gccccaggct ctgccacaac aagtctggct gctcctatca gcctgcacgt gtcatatgcc
     5641 ccaagacacg tgaccctgac cacactgatg gatacaggac ctggcagact gggcctgctc
     5701 ctgtgcagag ttgattccga tcctccagca cagctgcgac tgctccatgg cgatagactg
     5761 gttgcctcta ctctgcaagg cgttggcgga cctgaaggca gctctccaag actgcatgtc
     5821 gccgtggctc ccaacactct gagactcgaa attcacgggg ccatgctgga agatgaaggg
     5881 gtgtacattt gcgaggcctc taataccctg ggacaagcct ctgccagcgc cgactttgat
     5941 gcacaggcag tgaatgtcca agtgtggcct ggcgccacag tcagagaagg ccagctggtc
     6001 aatctgacat gcctcgtgtg gacaacacac cccgcacagc tgacctacac atggtatcag
     6061 gacggacagc agagactgga cgcccatagc atccctctgc ctaatgtgac tgtgcgggac
     6121 gccacctctt acagatgcgg agttggacct cctggacggg ctcctagact gagcagacct
     6181 atcactctgg acgtgctgta cgcccctcgg aacctgagac tgacatacct gcttgagtct
     6241 catggcggcc aactggccct ggtgctctgt actgttgata gcaggcctcc tgctcagctc
     6301 gctctgtctc atgctgggag actgctggcc tcttctaccg ccgcttctgt gcctaatact
     6361 ctgaggctgg aactgagagg ccctcagcct agagatgagg gcttttacag ctgcagcgct
     6421 agatctccac tcggccaggc taatacctct ctcgagctga gactggaagg cgtcagagtg
     6481 attctggctc ctgaagcagc tgttcccgaa ggcgccccta ttaccgtgac atgtgctgat
     6541 cctgccgctc acgcccctac actgtatacc tggtatcaca acggccggtg gctgcaagaa
     6601 ggacctgctg cctctctgag cttcctggtg gctacaagag cacacgctgg cgcctatagc
     6661 tgtcaggcac aagatgccca gggaaccaga tctagtagac cagccgctct gcaggttctg
     6721 tacgctccac aggatgccgt gctgagcagc ttcagagact ccagagccag atctatggcc
     6781 gtgatccagt gtaccgtgga ttctgagcct cctgccgaac tggcactgag ccacgatgga
     6841 aaagtgctgg caacatctag cggcgtgcac agccttgcct ctggaacagg acatgtgcag
     6901 gtcgccagaa acgccctgag acttcaggtg caagatgtgc ctgccggcga cgatacctat
     6961 gtgtgtacag cccagaatct gctgggctcc atcagcacaa tcggcagact gcaggttgag
     7021 ggcgccagag ttgttgccga acctggactt gatgtgccag aaggcgccgc tctgaacctg
     7081 tcttgtagac tgcttggagg ccccggacct gtgggcaata gcacattcgc ctggttctgg
     7141 aacgacagaa ggctgcacgc tgagcctgtg cctacactgg cctttacaca tgtggctaga
     7201 gcccaggcag gcatgtatca ctgtctggct gaactgccta caggcgctgc tgcttctgcc
     7261 cctgttatgc tgagagtgct ctacccacca aagactccca ccatgatggt gttcgttgag
     7321 cctgaaggcg ggctgagagg catcctggat tgcagagtgg actccgagcc actggcaagc
     7381 ctgacacttc atctgggaag cagactggtg gcatctagtc agcctcaagg cgctccagcc
     7441 gaacctcaca ttcacgtcct ggcctctcca aacgcactga gagtggatat tgaggccctg
     7501 aggccttccg accagggcga gtatatctgt agcgcctcca atgtgctggg aagcgcctct
     7561 accagcacat actttggcgt cagggcactg cacagactgc accagtttca gcaaggcgcg
     7621 ccggaagatc aagttgatcc aagattgatt gatggcaagg gcggatctct ggaggtgctg
     7681 ttccagggac caggcggaag cggcaatccc ggcggcgatc tggcccccca gatgctgcgc
     7741 gagctgcagg agaccaatgc cgccctgcag gacgtgcgcg agctgctgcg ccaccgcgtg
     7801 aaggagatca ccttcctgaa gaacaccgtg atggagtgcg acgcctgcgg tatgcagccc
     7861 tctagaggct ccggctccat catcccagtt gaggaggaga acccggactt ctggaaccgc
     7921 gaggcagccg aggccctggg tgccgccaag aagctgcagc ctgcacagac agccgccaag
     7981 aacctcatca tcttcctggg cgatgggatg ggggtgtcta cggtgacagc tgccaggatc
     8041 ctaaaagggc agaagaagga caaactgggg cctgagatac ccctggccat ggaccgcttc
     8101 ccatatgtgg ctctgtccaa gacatacaat gtagacaaac atgtgccaga cagtggagcc
     8161 acagccacgg cctacctgtg cggggtcaag ggcaacttcc agaccattgg cttgagtgca
     8221 gccgcccgct ttaaccagtg caacacgaca cgcggcaacg aggtcatctc cgtgatgaat
     8281 cgggccaaga aagcagggaa gtcagtggga gtggtaacca ccacacgagt gcagcacgcc
     8341 tcgccagccg gcacctacgc ccacacggtg aaccgcaact ggtactcgga cgccgacgtg
     8401 cctgcctcgg cccgccagga ggggtgccag gacatcgcta cgcagctcat ctccaacatg
     8461 gacattgacg tgatcctagg tggaggccga aagtacatgt ttcgcatggg aaccccagac
     8521 cctgagtacc cagatgacta cagccaaggt gggaccaggc tggacgggaa gaatctggtg
     8581 caggaatggc tggcgaagcg ccagggtgcc cggtatgtgt ggaaccgcac tgagctcatg
     8641 caggcttccc tggacccgtc tgtgacccat ctcatgggtc tctttgagcc tggagacatg
     8701 aaatacgaga tccaccgaga ctccacactg gacccctccc tgatggagat gacagaggct
     8761 gccctgcgcc tgctgagcag gaacccccgc ggcttcttcc tcttcgtgga gggtggtcgc
     8821 atcgaccatg gtcatcatga aagcagggct taccgggcac tgactgagac gatcatgttc
     8881 gacgacgcca ttgagagggc gggccagctc accagcgagg aggacacgct gagcctcgtc
     8941 actgccgacc actcccacgt cttctccttc ggaggctacc ccctgcgagg gagctccatc
     9001 ttcgggctgg cccctggcaa ggcccgggac aggaaggcct acacggtcct cctatacgga
     9061 aacggtccag gctatgtgct caaggacggc gcccggccgg atgttaccga gagcgagagc
     9121 gggagccccg agtatcggca gcagtcagca gtgcccctgg acgaagagac ccacgcaggc
     9181 gaggacgtgg cggtgttcgc gcgcggcccg caggcgcacc tggttcacgg cgtgcaggag
     9241 cagaccttca tagcgcacgt catggccttc gccgcctgcc tggagcccta caccgcctgc
     9301 gacctggcgc cccccgccgg caccaccgac gccgcgcacc cgggtggcgg aggactgaac
     9361 gacatcttcg aggctcagaa aatcgaatgg cacgaacatc accatcacca tcaccatcac
     9421 cattaaggcc ggcctgtagt cactggcaca acgcgttacc cggtaagcca atcgggtata
     9481 cacggtcgtc atactgcaga cagggttctt ctactttgca agatagtctt gagtagtaaa
     9541 ataaatagat agagaaaatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     9601 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     9661 taaaatgagg aaattgcatc acaacactca accctatctc ggtctattct tttgatttat
     9721 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     9781 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9841 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9901 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9961 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    10021 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
    10081 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
    10141 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
    10201 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatgac cgagtacaag
    10261 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
    10321 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
    10381 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
    10441 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
    10501 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
    10561 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
    10621 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
    10681 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac atccgcgccc
    10741 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
    10801 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacacgt gctacgagat
    10861 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    10921 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
    10981 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    11041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    11101 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcatt accaatgctt
    11161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    11221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gcgctgcgat
    11281 gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc agccagccgg
    11341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11461 cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    12001 aatactcata ttcttcctt
//
