LOCUS       sequence-389793-        9352 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0902
FEATURES             Location/Qualifiers
     source          1..9352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2916..4094
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3147..4085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4042..4062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4105..4114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5205..5793
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5258..5278)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5676..5687)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5865..6098
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6176..6297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6213..6232)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6267..6286
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6337..6472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6492..6511)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6493..6511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6521..6538)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6521..6537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6530..6552)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6679..7134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6766..6785)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6976..6997
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7160..7264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7265..7333,7334..8125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7483..7502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8296..8884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8785..8804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9038..9055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9172..9193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9208..9213,9214..9231,9232..9238)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9246..9262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9251..9273
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9270..9286
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9270..9286
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9305..9325
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9307..9325
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2881 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     2941 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     3001 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3061 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3121 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     3181 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     3241 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     3301 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     3361 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     3421 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     3481 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     3541 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     3601 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     3661 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     3721 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     3781 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     3841 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     3901 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     3961 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     4021 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     4081 ttcaggtgtc gtgaggctag caccgccacc atggacgcgg tgttgctgga gcacttcccc
     4141 gggggcctag acgcctttcc ttcttcgtac ttcgacgagg acgacttctt caccgaccag
     4201 tcttcacggg accccctgga ggacggcgat gagctgctgg cggacgagca ggccgaggtg
     4261 gagttcctta gccaccagct ccacgagtac tgctaccgcg acggggcgtg cctgctgctg
     4321 cagcccgcgc ccccggccgc cccgctagcg ctcgccccgc cgtcctcggg gggcctcggt
     4381 gagccagacg acggcggcgg cggcggctac tgctgcgaga cgggggcgcc cccaggcggc
     4441 ttcccctact cgcccggctc gccgccctcg tgcctggcct acccgtgcgc cggggcggca
     4501 gtactgtctc ccggggcgcg gctgcgcggc ctgagcggag cggcggctgc ggcggcgcgg
     4561 cgccggcggc gggtgcgctc cgaggcggag ctgcagcagc tgcggcaggc ggccaacgtg
     4621 cgcgagcggc ggcgcatgca gtccatcaac gacgccttcg aggggctgcg ctcgcacatc
     4681 cccacgctgc cctacgagaa gcgcctctcc aaggtggaca cgctgcgcct ggccatcggc
     4741 tacatcaact tcctcagcga gctcgtgcag gccgacctgc ccttgcgcgg cggtggcgcg
     4801 ggcggctgcg gggggccggg cggcggcggg cgcctgggcg gggacagccc gggcagccag
     4861 gcccagaagg tcatcatctg ccatcggggc acccggtccc cctcccccag cgaccctgat
     4921 tatggcctcc ctcccctagc aggacactct ctctcatgga ctgatgaaaa acaactcaag
     4981 gaacaaaata ttatccgaac agccaaagtc tggaccccag aggaccccag aaaactcaac
     5041 agcaaatctt ccttcaacaa catagaaaac gaaccaccat ttgagtttgt gtcctagtaa
     5101 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     5161 gacgacgtgt ccacctgtca ctggtatcta cgcgttaagt cgacaatcaa cctctggatt
     5221 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5281 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5341 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5401 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5461 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5521 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5581 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     5641 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5701 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5761 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     5821 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5881 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5941 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6001 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6061 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     6121 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6181 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6241 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6301 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6361 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6421 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     6481 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     6541 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     6601 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     6661 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6721 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6781 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6841 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6901 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6961 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7021 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7081 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7141 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7201 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7261 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7321 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7381 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7441 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7501 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7561 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7621 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7681 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7741 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7801 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7861 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7921 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7981 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8041 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8101 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8161 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8221 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8281 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8341 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8401 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8461 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8521 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8581 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8641 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8701 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8761 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8821 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8881 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8941 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9001 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9061 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9121 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9181 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9241 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     9301 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aa
//
