LOCUS       sequence-390241-        6803 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Drives specific expression in the ASK neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pCC20
FEATURES             Location/Qualifiers
     source          1..6803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3861..4010
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        4548..4652
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4653..4721,4722..5513)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4871..4890)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5684..6272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6173..6192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6426..6443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6560..6581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6596..6601,6602..6619,6620..6626)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6634..6650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6639..6661
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6658..6674
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6658..6674
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccggaa tggtctacgg acaaactggt
       61 ttactcatcg aaagactttg tgctactttt tcgaaagatt acaagaaaaa acaaagtgca
      121 attaaatgtg ctgttatttc aatacttgtt ctgatatgta gttcttcaac aggaagactg
      181 attgtatggg atgatcccat tgataaatat aattttgcat gctatattcc accaaaagaa
      241 agttatatta gggcaaatca ttattttaca atgtgtgctg tcctgtcgac tattaacttt
      301 tgtatttcta catttatttt gaaatacaat aaacgatgcg agtaccagtc agttttgttg
      361 gaattgaagt agcaatgata ataaaatttc agaactcgtt tcaaagttgg tgctcgtttt
      421 caaaagcaag aactgataga atcaacaaaa gccatttgtt tcttaacggt ttctcaattt
      481 gtggcagttt ttcttaattc attcggaatg attgttctcg tatatataca ggaaagtatt
      541 tctcatcgaa tattcaattt gttggttgta tggctttatg tcagttctac aaactcaaca
      601 ataaaactat aagaattagt atttcaggcg tttcctattg ttgttttgat gtttcctgtt
      661 atattagttc atcaaattcg tagcagtcga tggcgcagag ctttaaaaat taaagttata
      721 aagaatgaga agcagacgca ggatgatcat atgaagcata tgaagaatat gtggatttaa
      781 caatttttat tctgtttgta aattatattt tgtttccatc ctgaacactc cttctggttt
      841 cacatcggat tcaaaagtta atttatttat tcaaaatgtg tttttatttg agttgatgtt
      901 gtatccgtag attttcaatt ttaagttcgt tttgactgaa acatatttta ctggaattta
      961 ttcattttaa tatatgtcat gctttttgct tctgaatagg gaacctataa aggcaatcat
     1021 attacttttg ttttcgcttt caaaattgaa tttcccaatt ttcactgaca taattaacaa
     1081 ccaaaaaacc tgtctgaaga aaatcgtgtt ttccttttat ggcaagcgtg taaaattgaa
     1141 tctaacaaag agccggaaga aaattgaaaa gtttgatcaa cttagacgtc acgtggtttg
     1201 gtataaagac tattttcaat tcgaggtgtt cttataagaa tatattgaaa acaacgtttg
     1261 ttttgtcatg ttatgctgat tgattcgaaa aatatatttt gctttgaaac taaattgagc
     1321 tcaaacttat tataataaaa atgaattgaa aataaaaact ttccctattt tccctaacac
     1381 tttcataaat cccattaaat acttatattg taaatttttt tttagaaatc aacaatgtcg
     1441 tatgtaaagt gtgctccaga agaaatcata gaaagattcg aaagtataca tcaaaaatca
     1501 tctcaaattg tctatcttgc agcaataatt tccacattta ttacgacata ttttgctata
     1561 aaagttatct ttacccaatc ttttttcgaa atgtccacaa agattctatt agttcaaaac
     1621 ttattttaca cgaatttata tcaattgttc tacggatttg aagcaattgg aatgttgtac
     1681 agaagcttct ttctcacaaa tgagccttgc cgtcgtcaac acacattctg catcggaccg
     1741 aaaactgttg gcagtcttat cagcaagaaa atcgataaat ttcagagtcc gtttcaaagt
     1801 tcgagctcga ttccaaaagc aacagctgat cgagtcaact gaagccattt gttatttaac
     1861 actttcccag tttttggcag tctttgtaaa ttcatcgggt cttgtgataa tgtcctttat
     1921 ctgggacagt atgtctattc aattattcta cgctttgatc gtttggctct atgtcagttt
     1981 ttctgctatt tcactatttt caatataaaa ttttgcaggc gttccctgtt gttgcattga
     2041 tgttccccgt acttttgatt tatcaaattc gaaaaagtcg atcgcggaga gctctgaaga
     2101 ttaaagaaat taagaatgag aaacagacac aagatgatca tattaagcag atgactgaca
     2161 tgtggactta agttcgccag aaaaataagc ttttatgcag ttttatgtga aagtgattaa
     2221 ttgtttctat tttatcactg ttgaaaaata aaaacttaaa aatgtaaaaa aatctgcata
     2281 ttttttaaac tacttacagt gttgcaatac tgatatttcc gctttttaag caaattctaa
     2341 ataccattca ccagttaaaa agtgccgaaa acacatatct ccaaaacata aaaaatgaat
     2401 aaattattta tgttcaggta tggtgcatga aaaaaattaa caagtcatat aagacggagt
     2461 cgagcttcaa gttttggaaa aatacataat acgagctgat caagaagaaa caatatttct
     2521 ggtttaatgt gaaaacactt cacttccttt taatttcata tgccaaattt atttgcatct
     2581 ctattttata aatgttactg gaaaacataa aacagccatg attgttttca aaaaaggata
     2641 acaacttcaa acccgccaac aatggtgatc tctgatttcg aaattagctc aaaacgtcag
     2701 aaaattgttc tggtttggaa aaatgttcta catgaaaccc tcattagtat ttttttcaca
     2761 gtctgaaaga gatttttttc aaaatgtcta gaaccttctt tgtgattgaa cctgataagt
     2821 acatattcaa agcagtgttt ttgaaaattc atatgatttt ttaatgtaaa tctttcataa
     2881 gtgatcatat tctcagcaag ctattgaagc gcctagtaaa agtagcttat tcatatcgac
     2941 aatatattta aatccttttg atacaaaaaa ccatttttca ccatttgaat aatgtaaaaa
     3001 tccctcaagt ttaagaattt caagatttta acctgcaacg tgatcatttg ggttcagtca
     3061 actgaaaata atttcaaaat acgttttttt ttcatatttt cttattattt tcattttctg
     3121 aacttgtatt gtttgaaact atcaatgtca tttcaagtat taattttttt taaatatctc
     3181 ctaaagaact tttcttatca cttttgtatt tttcagtttc aagatttcgg cgcgcctcta
     3241 gaggatcccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac
     3301 atagaacatt ttcaggagga cccttggcta ggatgggccc taaaaagaag cgtaaagcta
     3361 gcatgaagct gttgtcttca atggaacaag cttgcgacat atgccggtta aaaaaactca
     3421 ggtgctcgaa ggaaaagccc aagtgtgcca agtgtctgaa gaacaactcg gagtgccgtt
     3481 attctcccaa ggctatgagg tccccactga ccagggcgca tctgacagag gtagaatcaa
     3541 ggttggaaaa attggaagat ttgtttctgc tcatgtttcc tcgggaaaac ctggacagca
     3601 ttttgaacat ggactctttg gacgaagtga aagtaatgtt gaaacaatta tatatgcagg
     3661 actatggtga cacggatgct gctatggata gcttgacccc tatggaaaat ggcatgtctg
     3721 aagggccgag aaaacacatg aagactgcga catcatcgcc aaaagtggat gaagatggaa
     3781 gccaaagtca gttgagtgta tcgccggaat tcccgctgca gtctggtggc ggaggggctc
     3841 ctaggggttc cggacgggct gacgcattgg acgattttga tctggatatg ctgggaagtg
     3901 acgccctcga tgattttgac cttgacatgc ttggttcgga tgcccttgat gactttgacc
     3961 tcgacatgct cggcagtgac gcccttgatg atttcgacct ggacatgctg attaactaag
     4021 gtaccgaatt cgctagccgg ccatacaagt aatccggatg atcgacgcca acgtcgttga
     4081 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     4141 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     4201 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     4261 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     4321 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     4381 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa
     4441 aaaataagca aagaaattag gcgaacgatg agaattgtcc tcgcttgggc ccaaaaggcc
     4501 tttttttttt ggcgccgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4621 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4681 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4741 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4801 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4861 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4921 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4981 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5041 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5101 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5161 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5221 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5281 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5341 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5401 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5461 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5521 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5581 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5641 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5701 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5761 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5821 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5881 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5941 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6001 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6061 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6481 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6541 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6601 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6661 gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa
     6721 ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata
     6781 cgctaacaac ttggaaatga aat
//
