LOCUS       sequence-390285-        9573 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian Expression of HsNot1iso1_1-1605-shRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: pT7-EGFP-C1-HsNot1iso1_1-1605-shRNAres_W
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        92..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        396..599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     550..570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     644..663
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        644..662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      690..699
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(696..698,699..701,702..1412)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(696..698,699..701,702..1412)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(741..762)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1002..1021)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1349..1370
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6392..6513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6429..6448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6483..6502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6520..6975)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6657..6678)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6869..6888
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7002..7106
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7108..7465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7108..7299
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7131..7151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7316..7451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7500..8294
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7500..8294
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7554..7573)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8164..8183
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8482..8501)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8526..8573
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8902..9490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9391..9410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      601 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      661 gggagaccca agctctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
      721 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      781 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      841 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      901 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      961 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1021 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1081 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1141 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1201 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1261 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1321 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1381 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga gctatgaatc
     1441 ttgactcgct ctcgctggcc ttgtctcaaa tcagctacct ggtggacaat ttaaccaaga
     1501 aaaattaccg agccagccag caggaaatac agcatattgt gaatcggcac ggtcctgagg
     1561 cagacaggca tttattacgc tgcctatttt cgcatgtgga tttcagtggc gatggtaaaa
     1621 gcagtggcaa agatttccat cagactcagt ttctgattca ggagtgtgcg ttgctgatta
     1681 caaagccaaa ttttatctcg acgctgtcct atgccattga taatccattg cactatcaga
     1741 agagtttaaa gcctgcaccc cacttatttg cccagctgag taaagtgctc aaattaagca
     1801 aagtacaaga ggtaattttt ggccttgccc tgttgaattc ttccagctca gatcttagag
     1861 gtttcgctgc ccagtttatc aaacagaagc ttccagatct tctgcgttct tacattgacg
     1921 cagacgtcag tggaaatcaa gaaggtggct tccaagatat agcaatagag gtcctacacc
     1981 tcctcctctc ccatctcctc tttgggcaga agggagcctt tggagttgga caagaacaga
     2041 tagacgcttt tcttaagacg ctgcgcagag attttcccca agaacgctgt cccgtggtgc
     2101 tcgcaccact tttataccct gaaaaacggg acattctaat ggacaggatc ctgcctgatt
     2161 ccggaggggt agctaaaacc atgatggaga gctctttggc tgatttcatg caagaagtag
     2221 gctatggctt ttgtgcaagt attgaagaat gtcgcaatat aatcgtgcag tttggtgttc
     2281 gggaggtcac agctgcccag gttgcaaggg ttttgggaat gatggctcga actcattcag
     2341 gattaacaga tggcattcca ttacagagta tttctgctcc gggcagtggg atctggagtg
     2401 atgggaaaga taaaagtgat ggagcacagg cacacacatg gaatgtagaa gtcttgattg
     2461 acgttcttaa agaactgaat ccaagtttga atttcaagga agtaacttat gaactggacc
     2521 atcctggatt tcaaattcgt gacagtaaag gacttcataa tgtggtttat ggcattcaga
     2581 ggggtttggg tatggaagtg ttcccagtag acctcatata tagaccttgg aaacatgctg
     2641 aaggccagct ctccttcatc cagcactcac taatcaatcc agagatcttc tgttttgctg
     2701 actatccctg tcatactgtt gccactgata ttctgaaagc accaccagag gatgacaatc
     2761 gagaaattgc cacatggaag agcttggatt tgattgaatc tctgctgagg cttgcagagg
     2821 ttgggcagta tgagcaagtc aaacagctct tcagcttccc tatcaaacac tgtccagaca
     2881 tgctggtatt ggccttacta caaattaaca cctcttggca taccttgcgc catgaactta
     2941 tctccactct gatgccaatt ttccttggaa accatcctaa ctcagctatt attttgcact
     3001 atgcatggca tgggcaggga cagtctccct caattcgcca acttatcatg catgcaatgg
     3061 cagaatggta catgagaggg gagcagtatg atcaggccaa attgtctcga atacttgatg
     3121 tggcccagga cttgaaggcc ttgtcaatgc tgctaaatgg tactccattt gcctttgtta
     3181 ttgaccttgc tgcacttgct tcacgtcgtg aatacctcaa acttgataag tggctcacag
     3241 ataaaattcg agagcatggg gagcctttta tccaggcgtg tatgactttt ttaaagagac
     3301 ggtgtccttc tattttgggc ggacttgccc cagaaaaaga ccagcccaaa agtgctcaac
     3361 ttcctccaga aactttggcg acaatgttgg cctgtctgca agcttgtgca gggagtgttt
     3421 ctcaggagct atcagaaact atcctcacca tggtagccaa ttgcagtaat gttatgaata
     3481 aggccagaca accaccacct ggagttatgc caaaaggacg tcctcctagt gctagcagct
     3541 tagatgccat ttctcctgtt cagattgacc ctcttgctgg aatgacatct cttagtatag
     3601 gtggttcagc tgcccctcac acccagagta tgcagggttt tcctccaaat ttgggttctg
     3661 cattcagtac ccctcagtca ccagcaaaag catttccacc cctttcaacc cccaatcaga
     3721 ccactgcatt cagtggtatt ggaggacttt catcacagct tccagtaggt ggtcttggca
     3781 caggcagcct gactggtata ggaactggtg ctcttggact ccctgcagtg aataacgacc
     3841 cttttgtaca gaggaaactg ggcacctctg gactgaatca gcctacattc cagcagagta
     3901 agatgaaacc ttcggacttg tctcaggtgt ggccagaggc aaaccagcac tttagtaaag
     3961 agatagatga tgaagcaaac agctatttcc agcgaatata taatcatcca ccacatccaa
     4021 ccatgtctgt tgatgaggta ttagaaatgc tgcagagatt taaagactct actataaaga
     4081 gggaacgaga agtatttaac tgtatgctaa ggaacttgtt tgaagaatat cgtttttttc
     4141 cccagtatcc tgataaagag ttacatataa cagcctgcct atttggtggt ataattgaga
     4201 aaggactggt cacttacatg gcactaggtc tggctctacg atatgttctt gaagccttac
     4261 gcaagccttt tggatccaaa atgtattatt tcgggattgc tgcactagat agatttaaaa
     4321 acagattgaa ggactatccc cagtattgtc agcatttggc ttctatcagt cactttatgc
     4381 aatttccaca tcatttacag gagtatattg agtatggaca gcagtctaga gatcctcctg
     4441 tgaaaatgca aggctctatc acaacccctg gaagtattgc actggctcag gcccaggctc
     4501 aggcccaggt tccagcaaaa gctcctcttg ctggtcaagt tagcactatg gtaaccacct
     4561 caacaactac cactgttgct aaaacggtta cggtcaccag gccaactgga gtcagcttta
     4621 agaaagatgt gccaccttct attaatacta caaatataga tacgttgctt gtggccacag
     4681 atcaaactga gagaattgtg gagcccccag aaaatatcca ggagaaaatt gcttttattt
     4741 tcaataatct ctcacagtca aatatgacac aaaaggttga agagctaaag gaaacggtga
     4801 aagaagaatt tatgccttgg gtttcacagt atctggttat gaagagagtc agtattgagc
     4861 caaactttca tagcctgtat tcaaacttcc ttgacacgct gaagaatcct gaatttaaca
     4921 agatggttct gaatgagacc tacagaaaca ttaaagtgct cctgacctct gataaagctg
     4981 cagccaattt ctcagatcgt tctttgctga agaacttggg acattggcta ggaatgatca
     5041 cattagctaa aaacaaaccc atcttacaca ctgacttgga tgtgaaatca ttgctgctag
     5101 aggcttatgt taaaggacaa caagaattgc tctatgtagt gccctttgtt gccaaagtct
     5161 tagaatctag cattcgtagt gtggttttta ggccaccaaa cccttggaca atggcaatta
     5221 tgaatgtatt agctgagcta catcaggagc atgacttaaa gttaaacttg aagtttgaaa
     5281 tcgaggttct ctgcaagaac cttgcattag acatcaatga gctaaaacct ggaaacctcc
     5341 taaaggataa agatcgcctg aagaatttag atgagcaact ctctgctcca aagaaagatg
     5401 tcaagcagcc agaagaactc cctcccatca caaccacaac aacttctact acaccagcta
     5461 ccaacaccac ttgtacagcc acggttccac cacagccaca gtacagctac cacgacatca
     5521 atgtctattc ccttgcgggc ttggcaccac acattactct aaatccaaca attcccttgt
     5581 ttcaggccca tccacagttg aagcagtgtg tgcgtcaggc aattgaacgg gctgtccagg
     5641 agctggtcca tcctgtggtg gatcgatcaa ttaagattgc catgactact tgtgagcaaa
     5701 tagtcaggaa ggattttgcc ctggattcgg aggaatctcg aatgcgaata gcagctcatc
     5761 acatgatgcg taacttgaca gctggaatgg ctatgattac atgcagggaa cctttgctca
     5821 tgagcatatc taccaactta aaaaacagtt ttgcctcagc ccttcgtact gcttccccac
     5881 aacaaagaga aatgatggat caggcagctg ctcaattagc tcaggacaat tgtgagttgg
     5941 cttgctgttt tattcagaag actgcagtag aaaaagcagg ccctgagatg gacaagagat
     6001 tagcaactga atttgagctg agaaaacatg ctaggcaaga aggacgcaga tactgtgatc
     6061 ctgttgtttt aacatatcaa gctgaacgga tgccagagca aatcaggctg aaagttggtg
     6121 gtgtggaccc aaagcagttg gctgtttacg aagagtttgc acgcaatgtt cctggcttct
     6181 tacctacaaa tgacttaagt cagcccacgg gatttttagc ccagcccatg aagcaagctt
     6241 gggcaacata gccgcgggcc cgggatccac cggatctaga taactgatca taatcagcca
     6301 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     6361 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     6421 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6481 ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt
     6541 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6601 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6661 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6721 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6781 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6841 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6901 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6961 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7021 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7081 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     7141 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7201 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7261 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7321 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7381 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7441 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     7501 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7561 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     7621 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7681 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7741 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7801 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7861 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7921 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7981 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     8041 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     8101 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     8161 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     8221 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     8281 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     8341 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8461 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     8521 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     8581 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     8641 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     8701 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     8761 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8821 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8881 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8941 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9001 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9061 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9541 tgctcacatg ttctttcctg cgttatcccc tga
//
