LOCUS       sequence-390362-         506 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Transient expression of NINJ1 gene  in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Borges JP, Saetra RSR, Volchuk A, Bugge M, Devant P, Sporsheim B, 
            Kilburn BR, Evavold CL, Kagan JC, Goldenberg NM, Flo TH, Steinberg 
            BE
  TITLE     Glycine inhibits NINJ1 membrane clustering to suppress plasma 
            membrane rupture in cell death.
  JOURNAL   Elife. 2022 Dec 5;11:e78609. doi: 10.7554/eLife.78609.
  PUBMED    36468682
REFERENCE   2  (bases 1 to 506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-5"; volume: "11:e78609. doi"; pages: " 
            10.7554/eLife.78609"
COMMENT     Sequence Label: pcDNA3.1-NINJ1
FEATURES             Location/Qualifiers
     source          1..506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..42
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggatccgccg ccaccatgga ctacaaagac gatgacgaca aggagtctgg caccgaggaa
       61 tatgagctga acggtgattt gcgccctggc tctcctggtt ctcctgatgc cctcccacca
      121 aggtgggggc ttcggaatcg ccctatcaat gtaaatcact acgcaaataa gaaatcagca
      181 gcagagtcta tgctggacat tgcactcttg atggctaacg cttctcagtt gaaggctgtg
      241 gtagagcaag gaaacgattt tgcatttttt gtccccttgg tggtattgat ttcaataagc
      301 ctggtacttc agattggagt cggggtactc ctgatcttct tggtgaaata tgacctcaac
      361 aatccagcca aacacgcaaa gctggatttt cttaataatc tcgcaaccgg actggtattt
      421 attatcgtag tcgttaacat ttttatcacc gcatttgggg tccagaagcc agtgatggat
      481 gtcgccccaa gacaataggc ggccgc
//
