LOCUS       sequence-39124-e         932 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(39..445)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    474..591
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(858..902)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgctcga ggatcgatcc gggcgactca gtctgtcgga
       61 ggactggcgc gccgagtgag gggttgtgag ctcttttata gagctcggga agcagaagcg
      121 cgcgaacaga agcgagaagc aggctgattg gttaattcaa ataaggcgca gggtcatttc
      181 aggtccttgg gggagcctgg aaacatctga tgggtcttaa gaaactgctg agggttgggc
      241 catatctggg gaccatctgt tcttggcctc gggccggggc cgaaactgcg gtgaccatct
      301 gttcttggcc ccgggccggg gccgaaactg ctcaccgcag atatcctgtt tggcccaacg
      361 ttagctattt tcatgtaccc gcccttgatc tgaacttctc tattcttggt ttggtatttt
      421 tccatgcctt gcaaaatggc gttactgcag ctagcacgtg acgcgtaatc gataaaattt
      481 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
      541 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa agttaaccga
      601 taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct
      661 tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat
      721 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
      781 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
      841 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
      901 ttttcttgct ggttttgcga ttcttcaatt aa
//
