LOCUS       sequence-391285-        8786 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Used for the expression and purification of EGFP labelled TBK1 
            carrying E696K mutation (SMC Internal No.: 1649).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, 
            Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn 
            M, Lindblom RSJ, Martens S, Lazarou M
  TITLE     Unconventional initiation of PINK1/Parkin mitophagy by Optineurin.
  JOURNAL   Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 
            10.1016/j.molcel.2023.04.021.
  PUBMED    37207627
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.04"; journalName: "Mol Cell"; date: 
            "2023-05-18- 18"; volume: "83"; issue: "10"; pages: "1693-1709.e9"
COMMENT     Sequence Label: pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K
FEATURES             Location/Qualifiers
     source          1..8786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(90..95,96..112,113..118))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    626..674
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     699..718
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(810..919)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        943..1034
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     959..976
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1077..1733
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MASPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1691..1713
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1740..1757
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1767..1787
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(1794..1796,1797..1799,1800..2510)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1794..1796,1797..1799,1800..2510)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1839..1860)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2100..2119)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2447..2468
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4842..4976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4879..4898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4933..4952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5005..5170
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5354..5808
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5441..5460)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5650..5671
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5834..5938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5939..6007,6008..6799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6157..6176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6970..7558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7459..7478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7712..7729
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7863..8087
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8154..8687)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
       61 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
      121 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
      181 aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc tccacgcatc
      241 gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg gatctgccct
      301 tgcttcagga gatcggtaga cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg
      361 atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc gcccaggact
      421 ctagctatag ttctagtggt tggctacgta cccgtagtgg ctatggcagg gcttgccgcc
      481 ccgacgttgg ctgcgagccc tgggccttca cccgaacttg ggggttgggg tggggaaaag
      541 gaagaaacgc gggcgtattg gtcccaatgg ggtctcggtg gggtatcgac agagtgccag
      601 ccctgggacc gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg ttttattctg
      661 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
      721 tagcctcccc catctcccgg taccgcatgc tatgcatcag ctgctagcac catggctcga
      781 gatcccgggt gatcaagtct tcgtcgagtg attgtaaata aaatgtaatt tacagtatag
      841 tattttaatt aatatacaaa tgatttgata ataattctta tttaactata atatattgtg
      901 ttgggttgaa ttaaaggtcc gtatactccg gaatattaat agatcatgga gataattaaa
      961 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
     1021 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatccatgg
     1081 catcccctat actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt
     1141 tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat
     1201 ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg
     1261 gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca
     1321 tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg
     1381 atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg
     1441 attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa
     1501 catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg
     1561 ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa
     1621 aacgtattga agctatccca caaattgata agtacttgaa atccagcaag tatatagcat
     1681 ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaatcggatc
     1741 tggttccgcg tggatcagga tcaggagaga atttatactt tcagggagcg cgcatggtga
     1801 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1861 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1921 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1981 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     2041 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2101 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2161 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2221 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2281 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2341 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2401 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2461 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtggag
     2521 gcatgcaatc cacatccaac cacctctggc tcctctcgga cattctcgga caaggcgcta
     2581 ccgctaacgt gttcagggga aggcacaaga agaccggtga cctgttcgcc atcaaggtgt
     2641 tcaacaacat ctcattcctg cgccctgtgg acgtccagat gcgtgagttc gaagtgctga
     2701 agaagctgaa ccacaagaac atcgtcaagc tgttcgccat cgaggaagaa accactaccc
     2761 gccacaaggt gctgatcatg gagttctgcc cctgcggttc cctgtacact gtcctggaag
     2821 agccttccaa cgcttacggt ctgcccgaga gcgagttcct gatcgtcctg cgtgacgtgg
     2881 tcggtggcat gaaccacctg agggaaaacg gcatcgtgca cagagacatc aagccaggaa
     2941 acatcatgcg cgtgatcggc gaggacggac agagcgtcta caagctgacc gacttcggcg
     3001 ctgccagaga gctggaagac gacgaacagt tcgtctctct gtacggaact gaagagtacc
     3061 tgcaccctga catgtacgag agggccgtgc tgagaaagga ccaccagaag aagtacggtg
     3121 ctaccgtgga cctgtggtct atcggcgtca ccttctacca cgctgccact ggatcactgc
     3181 ctttccgccc cttcgaaggt cctcgccgta acaaggaggt catgtacaag atcatcactg
     3241 gcaagccctc tggcgccatc tcaggagtcc agaaggctga aaacggtccc atcgactgga
     3301 gcggcgacat gccagtgagc tgctctctgt cccgcggtct gcaagtgctg ctgaccccag
     3361 tcctggccaa catcctggaa gctgaccagg agaagtgctg gggtttcgac cagttcttcg
     3421 ctgaaacctc cgacatcctg cacagaatgg tcatccacgt cttcagcctg caacagatga
     3481 ctgcccacaa gatttacatc cactcttaca acactgctac tatcttccac gaactggtct
     3541 acaagcagac caagatcatc tccagcaacc aggaactgat ctacgaggga aggagactgg
     3601 tgctggagcc cggtcgcctg gctcagcact tcccaaagac taccgaagag aaccctatct
     3661 tcgtggtgtc ccgcgagcca ctgaacacta tcggcctgat ctacgagaag atcagcctgc
     3721 ccaaggtcca cccacgttac gacctggacg gcgacgctag catggctaag gccatcaccg
     3781 gagtggtctg ctacgcttgc cgcatcgctt caactctgct gctgtaccag gaactgatgc
     3841 gcaagggaat ccgttggctg atcgagctga tcaaggacga ctacaacgaa actgtgcaca
     3901 agaagaccga ggtggtcatc actctggact tctgcatccg taacatcgaa aagaccgtga
     3961 aggtctacga gaagctgatg aagatcaacc tggaggctgc cgaactggga gagatcagcg
     4021 acatccacac taagctgctg cgcctgtctt catcccaggg cactatcgaa acctccctgc
     4081 aagacatcga ctcccgtctg agcccaggag gttcactggc tgacgcttgg gctcaccagg
     4141 aaggcaccca ccctaaggac aggaacgtgg agaagctgca agtcctgctg aactgcatga
     4201 ctgaaatcta ctaccagttc aagaaggaca aggccgagcg ccgtctggct tacaacgaag
     4261 agcagatcca caagttcgac aagcagaagc tgtactacca cgccaccaag gctatgactc
     4321 acttcaccga cgaatgcgtc aagaagtacg aggctttcct gaacaagagc gaagagtgga
     4381 tcaggaagat gctgcacctg agaaagcagc tgctgtccct gaccaaccag tgcttcgaca
     4441 tcgaagagga agtgagcaag taccaggagt acaccaacga gctgcaagag actctgcctc
     4501 agaagatgtt cactgccagc tctggtatca agcacactat gaccccaatc tacccttcat
     4561 ccaacaccct ggtggaaatg actctgggta tgaagaagct gaaggagaaa atggagggcg
     4621 tggtcaagga actggctgag aacaaccaca tcttggaaag attcggtagc ctgacgatgg
     4681 atggtggact gagaaacgtg gactgtctgt aaaagcttgt cgagaagtac tagaggatca
     4741 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4801 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     4861 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4921 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     4981 cactgcttga gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg
     5041 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     5101 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     5161 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact
     5221 ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct
     5281 gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg
     5341 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5401 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5461 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5521 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5581 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5641 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5701 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5761 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     5821 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5881 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5941 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6001 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6061 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6121 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6181 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6241 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6301 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6361 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6421 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6481 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6541 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6601 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6661 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6721 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6781 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6841 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7081 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7621 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7681 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7741 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     7801 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     7861 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     7921 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     7981 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     8041 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     8101 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     8161 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     8221 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     8281 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
     8341 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     8401 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     8461 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     8521 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     8581 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     8641 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca
     8701 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     8761 ctgtacaaaa aaacagtcat aacaag
//
