LOCUS       sequence-391431-       10174 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2165
FEATURES             Location/Qualifiers
     source          1..10174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6031..6619
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6084..6104)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6502..6513)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6691..6924
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7002..7123
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7039..7058)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7093..7112
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7163..7298
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7318..7337)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7319..7337)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7347..7364)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7347..7363)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7356..7378)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7505..7960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7592..7611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7802..7823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7986..8090
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8091..8159,8160..8951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8309..8328)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9122..9710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9611..9630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9864..9881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9998..10019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10034..10039,10040..10057,10058..10064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10072..10088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10077..10099
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10096..10112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10096..10112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10131..10151
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10133..10151
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtcg aatgaacttg atttcaggtc
     4141 tgtgcggctg ctaaagaacg acccagtcaa cttgcagaaa ttctcttaca ctagtgagga
     4201 tgaggcctgg aagacgtacc tagaaaaccc gttgacagct gccacaaagg ccatgatgag
     4261 agtcaatgga gatgatgaca gtgttgcggc cttgagcttc ctctatgatt actacatggg
     4321 tcccaaggag aagcggatat tgtcctccag cactgggggc aggaatgacc aaggaaagag
     4381 gtactaccat ggcatggaat atgagacgga cctcactccc cttgaaagcc ccacacacct
     4441 catgaaattc ctgacagaga acgtgtctgg aaccccagag tacccagatt tgctcaagaa
     4501 gaataacctg atgagcttgg agggggcctt gcccacccct ggcaaggcag ctcccctccc
     4561 tgcaggcccc agcaagctgg aggccggctc tgtggacagc tacctgttac ccaccactga
     4621 tatgtatgat aatggctccc tcaactcctt gtttgagagc attcatgggg tgccgcccac
     4681 acagcgctgg cagccagaca gcaccttcaa agatgaccca caggagtcga tgctcttccc
     4741 agatatcctg aaaacctccc cggaaccccc atgtccagag gactacccca gcctcaaaag
     4801 tgactttgaa tacaccctgg gctcccccaa agccatccac atcaagtcag gcgagtcacc
     4861 catggcctac ctcaacaaag gccagttcta ccccgtcacc ctgcggaccc cagcaggtgg
     4921 caaaggcctt gccttgtcct ccaacaaagt caagagtgtg gtgatggttg tcttcgacaa
     4981 tgagaaggtc ccagtagagc agctgcgctt ctggaagcac tggcattccc ggcaacccac
     5041 tgccaagcag cgggtcattg acgtggctga ctgcaaagaa aacttcaaca ctgtggagca
     5101 cattgaggag gtggcctata atgcactgtc ctttgtgtgg aacgtgaatg aagaggccaa
     5161 ggtgttcatc ggcgtaaact gtctgagcac agacttttcc tcacaaaagg gggtgaaggg
     5221 tgtccccctg aacctgcaga ttgacaccta tgactgtggc ttgggcactg agcgcctggt
     5281 acaccgtgct gtctgccaga tcaagatctt ctgtgacaag ggagctgaga ggaagatgcg
     5341 cgatgacgag cggaagcagt tccggaggaa ggtcaagtgc cctgactcca gcaacagtgg
     5401 cgtcaagggc tgcctgctgt cgggcttcag gggcaatgag acgacctacc ttcggccaga
     5461 gactgacctg gagacgccac ccgtgctgtt catccccaat gtgcacttct ccagcctgca
     5521 gcgctctgga ggggcagccc cctcggcagg acccagcagc tccaacaggc tgcctctgaa
     5581 gcgtacctgc tcgcccttca ctgaggagtt tgagcctctg ccctccaagc aggccaagga
     5641 aggcgacctt cagagagttc tgctgtatgt gcggagggag actgaggagg tgtttgacgc
     5701 gctcatgttg aagaccccag acctgaaggg gctgaggaat gcgatctctg agaagtatgg
     5761 gttccctgaa gagaacattt acaaagtcta caagaaatgc aagcgaggaa tcttagtcaa
     5821 catggacaac aacatcattc agcattacag caaccacgtc gccttcctgc tggacatggg
     5881 ggagctggac ggcaaaattc agatcatcct taaggagctg tagtaatgaa ctagtccgta
     5941 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aggtggtaac
     6001 gctgtaggaa tactcacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     6061 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6121 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6181 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6241 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6301 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6361 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6421 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6481 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6541 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6601 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     6661 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6721 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6781 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6841 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6901 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     6961 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7021 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7081 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7141 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7201 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7261 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7321 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7381 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7441 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7501 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7561 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7621 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7681 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7741 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7801 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7861 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7921 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7981 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8041 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8101 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8161 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8221 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8281 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8341 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8401 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8461 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8521 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8581 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8641 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8701 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8761 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8821 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8881 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8941 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9001 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9061 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9121 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9181 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9241 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9301 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9361 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9421 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9481 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9541 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9601 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9661 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9721 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9781 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9841 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9901 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9961 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    10021 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    10081 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    10141 ctcactaaag ggaacaaaag ctggagctgc aagc
//
