LOCUS       sequence-392929-        9890 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Retroviral expression of 
            SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)] protease reporter 
            and P2A firefly luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E
  TITLE     A highly sensitive NanoLuc-based protease biosensor for detecting 
            apoptosis and SARS-CoV-2 infection.
  JOURNAL   Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4.
  PUBMED    36720982
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-01-31"; volume: "13(1):1753. doi"; pages: " 
            10.1038/s41598-023-28984-4"
COMMENT     Sequence Label: 
            pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)]-Fluc
FEATURES             Location/Qualifiers
     source          1..9890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        108..487
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        488..691
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             692..867
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    930..1129
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1330..1746
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1676..1698
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1714..1730
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1783..2086
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2087..2290
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2365..2374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2755..2808
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2815..2824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3313..3360
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             3364..3483
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             3499..3555
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3562..5214
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    5329..5791
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5385..5402)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5612..5631
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5817..6416
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5817..5836)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6313..6333
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6775..7200
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7303..7325)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7461..7481)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7466..7795
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7646..7781
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7817..7836
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7917..7934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8088..8676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8168..8187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8847..9638,9639..9707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9470..9489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9708..9812)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(9880..9890,1..8)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacgaggccc tttcgtcttc aagaattagc ttggccattg catacgttgt atccatatca
       61 taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt
      121 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      181 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      241 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      301 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      361 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      421 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      481 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      541 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      601 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      661 tgtacggtgg gaggtctata taagcagagc tcaataaaag agcccacaac ccctcactcg
      721 gcgcgccagt cttccgatag actgcgtcgc ccgggtaccc gtattcccaa taaagcctct
      781 tgctgtttgc atccgaatcg tggtctcgct gttccttggg agggtctcct ctgagtgatt
      841 gactacccac gacgggggtc tttcatttgg gggctcgtcc gggatttgga gacccctgcc
      901 cagggaccac cgacccacca ccgggaggta agctggccag caacttatct gtgtctgtcc
      961 gattgtctag tgtctatgtt tgatgttatg cgcctgcgtc tgtactagtt agctaactag
     1021 ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg ccgcaaccct
     1081 gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg aagggagtcg
     1141 atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa cctaaaacag
     1201 ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg
     1261 ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct
     1321 gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
     1381 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
     1441 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
     1501 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
     1561 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
     1621 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
     1681 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
     1741 ggcgccggaa ttgaagatct gggggatcga tcctctagag tccgttacat aacttacggt
     1801 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1861 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1921 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1981 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2041 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2101 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2161 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2221 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2281 aagcagagct cgtttagtga accgattaaa ggtttatacc ttcccaggta acaaaccaac
     2341 caggcctcgt acgcttaatt aacggccacc atgggctccc tgctgttccg agtaaccatc
     2401 aacggcggcg gtggaggctc cgggggaggc gggtctggcg gagggggaag tggcggtggt
     2461 ggctctgaag ttagcgctct ggaaaaagaa gtgtctgcac tcgagaaaga agtaagtgcc
     2521 cttgagaagg aggtgtccgc actcgagaag gaggtcagcg ccctggaaaa ggaaaaggtg
     2581 agtgggtggc gacttttcaa aaaaatctcc ggatcctcag tggccgtgag cgctagcggc
     2641 ttcaaggtgt ccgccctgaa ggaaaaagta agcgcactga aagaaaaggt gagcgcgctg
     2701 aaggagaaag tgagcgccct gaaagagaaa gtctctgccc ttaaggagga attcgagggc
     2761 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaaa gcttgccacc
     2821 atggttttca cattggaaga cttcgtgggt gactgggagc agactgccgc gtacaacttg
     2881 gatcaagttc tggagcaagg tggagtaagc tccctcctgc aaaaccttgc ggtaagcgtt
     2941 actcccatcc aaaggatcgt aaggtccggg gaaaatgctc tcaaaataga tatacacgtt
     3001 atcatacctt acgaagggct gtctgccgat cagatggctc aaatagaaga agttttcaaa
     3061 gtagtctacc ccgtggacga tcatcatttt aaggtgattc tcccttatgg gaccctcgtt
     3121 atcgacgggg ttacacctaa tatgctcaat tacttcggaa ggccatatga aggaatagct
     3181 gttttcgatg gtaaaaagat tacggtcacc ggcactctct ggaatgggaa taagataata
     3241 gatgagagac tgataacgcc agacggcgtg gccgtgagcg ctagcggctt ccaattggga
     3301 ggcagcgaag tggcttgcaa gaactggttc agtagcttaa gccactttgt gatccacctt
     3361 aattctcacg gctttccgcc tgaggttgaa gagcaagccg ccggtacatt gcctatgtcc
     3421 tgcgcacaag aaagcggtat ggaccggcac ccagccgctt gtgcttcagc tcgcatcaac
     3481 gtcggcggcc gctctagagc aaccaatttc agccttctta aacaggctgg agatgtggaa
     3541 gagaatcctg gaccagaatt gatggaagat gccaaaaaca ttaagaaggg cccagcgcca
     3601 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
     3661 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
     3721 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
     3781 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
     3841 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
     3901 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
     3961 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     4021 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     4081 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     4141 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     4201 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     4261 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     4321 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     4381 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     4441 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     4501 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     4561 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     4621 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     4681 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     4741 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     4801 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     4861 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     4921 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     4981 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     5041 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     5101 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     5161 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gtaaccgccc
     5221 ccccccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     5281 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     5341 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     5401 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     5461 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     5521 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     5581 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     5641 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     5701 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     5761 cgtggttttc ctttgaaaaa cacgatgata agcttgccac aaggagacga ccttccatga
     5821 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     5881 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     5941 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6001 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6061 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6121 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6181 cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6241 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6301 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6361 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatgct
     6421 tgcaggcatc tcatgagtgt gtcttcccgt tttccgcctg aggtcactgc gtggatggag
     6481 cgctggcgcc tgctgcgcga cggcgagctg ctcaccaccc actcgagggc tgcagcgctg
     6541 cagaggccga gtgcagaact gctccaaagg gacctcaagg ctttccgagg gacactaggc
     6601 tgactccatc gagccagtgt agagataagc ttatcgatta gtccaatttg ttaaagacag
     6661 gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga
     6721 gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     6781 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     6841 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagggtc
     6901 gaccctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     6961 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     7021 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     7081 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     7141 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
     7201 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
     7261 gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7321 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7381 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     7441 cggagtgtag atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7561 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7621 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7681 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7741 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7801 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7861 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7921 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7981 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8041 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8101 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8161 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8221 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8281 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8341 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8401 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8461 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8521 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8701 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8761 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8821 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8881 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8941 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9001 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9061 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9121 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     9181 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9241 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9301 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9361 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9421 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     9481 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9541 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9601 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9661 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9721 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9781 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9841 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
//
