LOCUS       sequence-392968-        9485 bp    DNA     circular SYN 14-APR-2025
DEFINITION  QUAS reporter line expressing GCaMP7s calcium indicator and 
            tdTomato, separated by T2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Zhao Z, Zung JL, Hinze A, Kriete AL, Iqbal A, Younger MA, Matthews 
            BJ, Merhof D, Thiberge S, Ignell R, Strauch M, McBride CS
  TITLE     Mosquito brains encode unique features of human odour to drive host 
            seeking.
  JOURNAL   Nature. 2022 May;605(7911):706-712. doi: 10.1038/s41586-022-04675-4.
            Epub 2022 May 4.
  PUBMED    35508661
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04675-4"; journalName: "Nature"; date: 
            "2022-05"; volume: "605"; issue: "7911"; pages: "706-712"
COMMENT     Sequence Label: QUAS-jGCaMP7s-T2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..9485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      1294..1303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1300..1302,1303..1305,1306..2019)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     1953..1974
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2142..2263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2675..2913
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2860..2881
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             2998..3015
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(3055..3063,3064..3078)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             4348..4401
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4411..5838
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     intron          5915..5980
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6110..6130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6402..6536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6811..6845
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7336..7352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7336..7352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7349..7371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7360..7376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7384..7390,7391..7408,7409..7414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7429..7450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7567..7584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7738..8326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7818..7837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8497..9288,9289..9357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9120..9139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9358..9462)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt
      961 ctataaagta acaaaacttt tatcgaattc ctgcagcccg ggggatccac tagttctagt
     1021 gttcccacaa tggttaattc gagctcgccc ggggatctaa ttcaattaga gactaattca
     1081 attagagcta attcaattag gatccaagct tatcgatttc gaaccctcga ccgccggagt
     1141 ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac
     1201 gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatcgggg
     1261 taccgctaga gtcgacggta cgatccaccg gtcgccacca tggtgagcaa gggcgaggag
     1321 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1381 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1441 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
     1501 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1561 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1621 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1681 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
     1741 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     1801 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1861 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1921 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1981 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     2041 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2101 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2161 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2221 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaagctta tcgatacgcg
     2281 tacggcacta gagcggccgc caccgcggtc acgtgtcact gggtcagtgg gtaatcgctt
     2341 atcctcggat aaacaattat cctcacgggt aatcgcttat ccgctcgggt aatcgcttat
     2401 cctcgggtaa tcgcttatcc ttgggtaatc gcttatcctc ggataaacaa ttatcctcac
     2461 gggtaatcgc ttatccgctc gggtaatcgc ttatcctcgg gtaatcgctt atccttgggt
     2521 aatcgcttat cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa
     2581 tcgcttatcc tcgggtaatc gcttatcctt tcacgttggg actcagtgag gaggacctga
     2641 attcctgcag cccgagcgga gactctagcg agcgccggag tataaataga ggcgcttcgt
     2701 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
     2761 gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt
     2821 gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa
     2881 gaagtaatta ttgaatacaa gaagagaact ctgaataggg aattgggaat tcgttaacag
     2941 atcgggggat caattcgtta acagatctgc ggccgcggct cgagcaaaat gggctcacat
     3001 catcaccacc accacggaat ggctagcatg acgggcggac aacagatggg ccgcgatctg
     3061 tacgatgatg atgataagga cctggcgaca atggttgaca gcagccggcg caagtggaac
     3121 aaaacaggac atgcagtcag agtcattggc cgcctttcct cactggaaaa cgtctacata
     3181 aaggcggata agcagaagaa cggtatcaaa gccaatttcc atatccgcca caacatcgaa
     3241 gatggcggag tgcagctcgc ataccactac caacagaata cgccaatcgg agacggcccc
     3301 gtgctgctgc ccgacaacca ttacctgagt gttcagtcga agcttagcaa ggacccaaac
     3361 gaaaaacgag atcatatggt tctgctggaa tttgtgactg ctgctggtat taccctgggc
     3421 atggatgaac tctataaggg tggaacggga ggctccatgg ttagcaaagg cgaggagctg
     3481 ttcactggcg tcgtgccgat cctggtcgag ttggacggag atgttaatgg acacaaattc
     3541 agtgtgagtg gcgagggcga gggcgatgcg acatacggca agttgacctt gaagttcatt
     3601 tgcaccaccg gtaaactgcc agtaccctgg cccacgctgg tcaccacgct cacctatggc
     3661 gtccagtgct tctctcgtta tccggatcac atgaagcagc atgatttctt caagtccgcc
     3721 atgcctgagg ggtacattca agagcgaaca atcttcttca aggacgacgg caattacaag
     3781 accagagccg aagtgaagtt tgaaggcgat acactagtga accgcatcga gctaaagggt
     3841 attgatttta aggaggatgg caacatcttg ggccacaagc tggagtacaa ccttcccgat
     3901 cagctaaccg aggagcagat cgcggagttc aaggaactgt tttcgctgtt cgacaaagat
     3961 ggtgatggta cgattaccac gaaggaattg ggaacagtga tgcggtccct ggggcagaac
     4021 cccaccgagg ccgagctgca ggatatgata aacgaggtgg atgctgatgg tgacggcacg
     4081 attgattttc ccgagttctt gaccatgatg gcccgaaaga tgaagtaccg cgatacagag
     4141 gaggagatta gggaggcatt cggtgtgttc gacaaggacg gcaatggata cattagtgca
     4201 gcagagttgc gccacgttat gactaacctg ggtgagaagt tgactgacga agaggtcgat
     4261 gagatgatta gggaagccga tattgacggc gatggccagg tgaactatga ggaattcgta
     4321 caaatgatga ccgccaaagg atcgggagag ggccgcggct ccctgctgac ctgcggcgac
     4381 gtggaggaga accccggccc ctccggatcg atggtgagca agggcgagga ggtcatcaaa
     4441 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     4501 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4561 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     4621 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     4681 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     4741 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4801 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4861 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4921 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4981 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     5041 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc
     5101 ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc
     5161 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
     5221 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     5281 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     5341 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
     5401 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
     5461 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
     5521 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     5581 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     5641 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     5701 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     5761 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc
     5821 atggacgagc tgtacaagga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     5881 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     5941 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     6001 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     6061 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     6121 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     6181 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     6241 actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga ctagagatca
     6301 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     6361 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     6421 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6481 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgata
     6541 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
     6601 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
     6661 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
     6721 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
     6781 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
     6841 tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc
     6901 gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg
     6961 ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa
     7021 aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac
     7081 tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga
     7141 tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg
     7201 ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt
     7261 atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg
     7321 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7381 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7441 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7501 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7561 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7621 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7681 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7741 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7801 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7861 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7921 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7981 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8041 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8101 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8161 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8221 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8281 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8341 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8401 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8461 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8521 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8581 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8641 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8701 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8761 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8821 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8881 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8941 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9001 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9061 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9121 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9181 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9241 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9301 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9361 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9421 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9481 gccac
//
