LOCUS       sequence-392971-        8823 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of Sox2 with Sox13 DBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: Halo-S2S13DBD
FEATURES             Location/Qualifiers
     source          1..8823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(261..1052,1053..1121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     884..903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1122..1226)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1252..1707)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1389..1410)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1601..1620
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1834..1856
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1848..1865
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1849..1865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1995..2057
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2510..2721
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2734..3002
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3034..3099
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3109..4002
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTTLEISG"
     CDS             4015..4035
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    5017..5589
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5184..5201)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5411..5430
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5598..6392
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVPYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5652..5671)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6262..6281
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6452..6472)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6514..6635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6551..6570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6605..6624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7398..7432
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7923..7939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7923..7939)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7936..7958)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7947..7963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7971..7977,7978..7995,7996..8001))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8016..8037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8154..8171)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8325..8823,1..90))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8405..8424)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
       61 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      541 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      601 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      661 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      721 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      781 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      841 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      901 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      961 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1021 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1081 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1141 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1201 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     1261 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1321 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     1381 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     1441 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     1501 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     1561 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     1621 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     1681 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     1741 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     1801 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1861 ccagtgagcg cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagactcgcg
     1921 gtgcaaatgt gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca
     1981 cgcagctaga ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
     2041 tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
     2101 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
     2161 tatttatgtt tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     2221 caaaactttt atgagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
     2281 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
     2341 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
     2401 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
     2461 aaaaggcctc cacggccaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     2521 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     2581 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     2641 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     2701 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     2761 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     2821 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     2881 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     2941 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     3001 tccaagctgt gaccggcgcc tactctagcc atggactaca aagaccatga cggtgattat
     3061 aaagatcatg acatcgatta caaggatgac gatgacaagc ttggctccat ggcagaaatc
     3121 ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac
     3181 gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc
     3241 tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca
     3301 gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac
     3361 gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt
     3421 cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa
     3481 ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt
     3541 gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat
     3601 cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc
     3661 gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc
     3721 ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa
     3781 tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc
     3841 gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct
     3901 gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc
     3961 gagatcgcgc gctggctgtc gacgacgctc gagatttccg gcgagccaac cactgaggat
     4021 ctgtactttc agagcgataa ctctagaatg tataacatga tggagacgga gctgaagccg
     4081 ccgggcccgc agcaagcttc ggggggcggc ggcggaggag gcaacgccac ggcggcggca
     4141 accggcggca accagaagaa cagcccggac cgcatcaaga gacccatgaa tgccttcatg
     4201 gtgtgggcca aggatgaacg gaggaaaatt ctccaggcct tcccagacat gcataactcc
     4261 agcatcagca agatccttgg ttctcgctgg aagtccatga ccaaccagga gaaacagccg
     4321 tactacgagg agcaggcccg tctgagccgg cagcacctgg agaaataccc tgactataag
     4381 taccggccgc ggcggaaaac caagacgctc atgaagaagg ataagtacac gcttcccgga
     4441 ggcttgctgg cccccggcgg gaacagcatg gcgagcgggg ttggggtggg cgccggcctg
     4501 ggtgcgggcg tgaaccagcg catggacagc tacgcgcaca tgaacggctg gagcaacggc
     4561 agctacagca tgatgcagga gcagctgggc tacccgcagc acccgggcct caacgctcac
     4621 ggcgcggcac agatgcaacc gatgcaccgc tacgacgtca gcgccctgca gtacaactcc
     4681 atgaccagct cgcagaccta catgaacggc tcgcccacct acagcatgtc ctactcgcag
     4741 cagggcaccc ccggtatggc gctgggctcc atgggctctg tggtcaagtc cgaggccagc
     4801 tccagccccc ccgtggttac ctcttcctcc cactccaggg cgccctgcca ggccggggac
     4861 ctccgggaca tgatcagcat gtacctcccc ggcgccgagg tgccggagcc cgctgcgccc
     4921 agtagactgc acatggccca gcactaccag agcggcccgg tgcccggcac ggccattaac
     4981 ggcacactgc ccctgtcgca catgtgagcg gccgcgcccc tctccctccc ccccccctaa
     5041 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     5101 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5161 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5221 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5281 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5341 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     5401 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     5461 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     5521 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5581 acgataatac ctccggaatg attgaacaag atggattgca cgcaggttct ccggccgctt
     5641 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5701 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5761 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc gcgacgggcg
     5821 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     5881 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     5941 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6001 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6061 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6121 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6181 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6241 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6301 aatgggctga ccgcttcctc gtgccttacg gtatcgccgc tcccgattcg cagcgcatcg
     6361 ccttctatcg ccttcttgac gagttcttct gagtcgacaa tcaacctctg gattacaaaa
     6421 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6481 ctgctttaat gcctttgtat catgcgttaa ctaaacttgt ttattgcagc ttataatggt
     6541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggaattg actcaaatga
     6661 tgtcaattag tctatcagaa gctcatctgg tctcccttcc gggggacaag acatccctgt
     6721 ttaatattta aacagcagtg ttcccaaact gggttcttat atcccttgct ctggtcaacc
     6781 aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag tggcagccag
     6841 gtttagcccc ggaattgact ggattccttt tttagggccc attggtatgg ctttttcccc
     6901 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     6961 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     7021 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
     7081 gacgtaatta catccctggg ggctttgggg gggggctgtc cctgatatct ataacaagaa
     7141 aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta
     7201 tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc
     7261 gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca
     7321 agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga
     7381 gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatcta
     7441 gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg
     7501 ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg
     7561 gaaagagttt gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac
     7621 catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc
     7681 cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa
     7741 gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat
     7801 taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat
     7861 gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgctt ggcgtaatca
     7921 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7981 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8041 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8101 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8161 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8221 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8281 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8341 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8401 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8461 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8521 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8581 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8641 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8701 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8761 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8821 ggt
//
