LOCUS       sequence-393047-       15908 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15908)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 15908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-EGFP-iCre
FEATURES             Location/Qualifiers
     source          1..15908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3321..3330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3327..3329,3330..3332,3333..4046)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3327..3329,3330..3332,3333..4043)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3372..3393)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3633..3652)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3980..4001
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      4083..4092
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4095..4115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7337..7348
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9140..9151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12347..12365)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12361..12408
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12436..12452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12436..12452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12449..12471)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12460..12476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12484..12490,12491..12508,12509..12514))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12529..12550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12667..12684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12838..13426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12918..12937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13597..14388,14389..14457))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14220..14239
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14458..14562)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14589..15045
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14676..14695)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14886..14907
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15171..15193
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15185..15202
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15186..15202
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15272..15575
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15576..15779
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15730..15750
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15824..15843
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15824..15842
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggcgtac gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     3361 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3421 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     3481 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     3541 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     3601 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     3661 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     3721 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     3781 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     3841 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3901 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     3961 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4021 tcggcatgga cgagctgtac aagtaagcta gacatgaaaa aaactaacac tcctccggta
     4081 ccgccaccat ggtgcccaag aagaagagga aagtctccaa cctgctgact gtgcaccaaa
     4141 acctgcctgc cctccctgtg gatgccacct ctgatgaagt caggaagaac ctgatggaca
     4201 tgttcaggga caggcaggcc ttctctgaac acacctggaa gatgctcctg tctgtgtgca
     4261 gatcctgggc tgcctggtgc aagctgaaca acaggaaatg gttccctgct gaacctgagg
     4321 atgtgaggga ctacctcctg tacctgcaag ccagaggcct ggctgtgaag accatccaac
     4381 agcacctggg ccagctcaac atgctgcaca ggagatctgg cctgcctcgc ccttctgact
     4441 ccaatgctgt gtccctggtg atgaggagaa tcagaaagga gaatgtggat gctggggaga
     4501 gagccaagca ggccctggcc tttgaacgca ctgactttga ccaagtcaga tccctgatgg
     4561 agaactctga cagatgccag gacatcagga acctggcctt cctgggcatt gcctacaaca
     4621 ccctgctgcg cattgccgaa attgccagaa tcagagtgaa ggacatctcc cgcaccgatg
     4681 gtgggagaat gctgatccac attggcagga ccaagaccct ggtgtccaca gctggtgtgg
     4741 agaaggccct gtccctgggg gttaccaagc tggtggagag atggatctct gtgtctggtg
     4801 tggctgatga ccccaacaac tacctgttct gccgggtcag aaagaatggt gtggctgccc
     4861 cttctgccac ctcccaactg tccacccggg ccctggaagg gatctttgag gccacccacc
     4921 gcctgatcta tggtgccaag gatgactctg ggcagagata cctggcctgg tctggccact
     4981 ctgccagagt gggtgctgcc agggacatgg ccagggctgg tgtgtccatc cctgaaatca
     5041 tgcaggctgg tggctggacc aatgtgaaca ttgtgatgaa ctacatcaga aacctggact
     5101 ctgagactgg ggccatggtg aggctgctcg aggatgggga cggcagtgga ggatccggat
     5161 gagctagcgg cgcctagccg gtcatccttt tgacgattcc agtcccgagg ataacctcct
     5221 ctcgggattg gggggaatct ttggatccag tagtcctcct tgaactccgt ccaacagggc
     5281 agattcaaga gtcataagac tttcattaat catttcagtt gatcagacat ggtcgtgtag
     5341 attctcttaa tacgggagat cttctagcag tttcagtgac caacggtgct ttcattctcc
     5401 aggaactgat accaaaggtt gtggacaggc caaggggtac ttcggatgac tctgtgcttg
     5461 ggcacagaaa gaggtcgtag tgtgcccccc gatagcggac tcgacatgaa tcaactaaga
     5521 aaggcaatct gcctcccatg atggacataa gcaatagttc acaaccatct tgcatctcag
     5581 tgaagtgtac ataactattg agggctgggt catctaagca tttcagtcga gaaaaaaact
     5641 gtagaccaaa agaacaacta gcaacacttc tcatccagag acccatatca agatgctaga
     5701 tccgggagag gtttatgatg accctattga tccaattgag tcagaggctg aacccagagg
     5761 aacccccact gtccccaaca tcttgaggaa ctccgactac aatctcaatt ctcctttgat
     5821 agaggatcct gccaaactaa tgttagaatg gttgaagaca gggaacagac cttatcggat
     5881 gactttgaca gacaattgct ccaggtctta caaagttttg aaagattatt tcaagaaagt
     5941 agatttgggt tctctcaaag tgggcggaac tgctgcacag tcaatggttt ctctctggtt
     6001 gtgtggtgcc cactctgaat caaacaggag ccggagatgt ataaccgact tggcccattt
     6061 ctattccaag tcatccccca tagagaagct attgaattgt acgctaggaa acagaggcct
     6121 gagaatccca ccagaggggg tgttaaattg cctcgagagg gtcaattatg acaaggcatt
     6181 tgggaggtat ctggccaaca cgtattcctc ttacttgttt ttccatgtaa tcaccttata
     6241 catgaatgcc ttagactggg aagaggaaaa gaccatcctg gcattatgga aagatataac
     6301 ctcagtggat accgagaagg acttggtcaa attcaaagat caaatatggg gactgttgat
     6361 tgtgacaaag gactttgttt actctcagag ttcaaactgt ctttttgaca gaaactacac
     6421 actgatgcta aaggatcttt tcttgtctcg attcaactcc ttaatgattc tgctttctcc
     6481 ccctgagccc cgatactcag atgacttaat atctcagctg tgccagctat acatcgctgg
     6541 ggatcaagtc ttgtccatgt gtgggaactc cggctatgaa gtcatcaaaa tattggagcc
     6601 atatgtcgtg aacagtttgg tccagagggc agagaagttt aggcctctca tccacccctt
     6661 gggagacttt cctatgttca taaaagacaa ggtgaatcaa cttgaaggga cttttggtcc
     6721 cagtgcaaag aggtttttta gggttttaga tcaattcgac aacatacatg acttagtatt
     6781 tgtgtatggc tgttacagac attgggggca cccctatata gattatcgga agggtctgtc
     6841 gaaactatat gatcaagttc acattaagaa agtaatagat aagtcctacc aggagtgttt
     6901 agcaagtgac ttggccagaa ggatcctcag atggggattt gacaagtact ccaaatggta
     6961 tctagattcg agattccttg cccgagacca ccccttgact ccttatgtca agacccaaac
     7021 atggccaccc aaacatatag tagacttggt gggggacaca tggcataagc tcccgatcac
     7081 gcagatcttt gaaattcctg aatcaatgga cccgtcagag atactggatg acaaatcaca
     7141 ttctttcacc agaacaaggt tagcttcttg gctgtcagag aaccgagggg ggcctgttcc
     7201 tagcgagaag gtcattatca cggccctgtc taagccacct gtcaatcccc gagagttttt
     7261 gaaatctata gacctcggag gattgccaga tgaggatttg ataattggcc tcaaaccaaa
     7321 ggaacgggag ttgaagatcg agggccgatt ctttgctcta atgtcatgga atctaagatt
     7381 atattttgtc atcaccgaaa agctcttggc caactacatt ttgccacttt ttgacgcact
     7441 gactatgaca gacaacctga acaaggtgtt caaaaagttg atcgacaggg tcaccgggca
     7501 agggcttttg gactattcta gggtcacata cgcatttcac ctggactatg agaaatggaa
     7561 caatcatcaa agattggagt caacagagga tgtattctct gtcctagatc aggtgtttgg
     7621 attgaagagg gtgttttcta gaacacacga gttttttcag aagtcctgga tctattattc
     7681 agacagatca gacctcattg ggttatggga ggaccaaata tattgcttgg atatgtctaa
     7741 cggcccaacc tgctggaatg gccaagatgg cgggctagag ggcttgcggc agaagggctg
     7801 gagtctagtc agtttattaa tgatagatag agaatctcaa accaggaaca caagaaccaa
     7861 gatactagct caaggagaca accaggttct gtgtcctaca tacatgttgt caccgggatt
     7921 gtctcaagag gggcttctct atgagttaga gagcatatca aggaatgcac tctcaatata
     7981 ccgagctatc gaggaaggag catctaagct ggggctgatc atcaagaagg aagagaccat
     8041 gtgtagttat gactttctca tatatgggaa gaccccctta tttcgaggca acatattggt
     8101 gcctgaatcc aaaagatggg cccgagtctc ttgcatctct aacgaccaaa tagtcaacct
     8161 cgccaatata atgtcgacag tatccaccaa tgcgctgaca gtggcacaac actctcaatc
     8221 tctgatcaaa cctatgaggg attttctgct catgtcagta caggcagttt tccactacct
     8281 gttgtttagc ccaatcctaa aaggcagagt ttataagatt ctgagtgctg aaggggagag
     8341 ctttctccta gccatgtcgc ggataatcta cctagatcct tctttgggag gggtgtctgg
     8401 aatgtctctc gggaggttcc atatacgtca gttctcagac cctgtctctg aagggttgtc
     8461 attctggaga gagatctggt taggctctca tgagtcctgg attcacgcgt tgtgtcagga
     8521 ggccgggaac cccgatcttg gagagagaac actcgagagc ttcactcgcc ttttagaaga
     8581 tcctactacc ttaaatatca aaggaggggc cagccctacc attctactca aggatgctat
     8641 cagaaaggct ctgtacgacg aggtggacaa ggtggaaaat tcagagtttc gagaggcaat
     8701 cctgctgtcc aagacccata gagataactt tatactcttt ttaaaatctg ttgagcctct
     8761 gttccctcga tttctcagtg agctcttcag ttcgtctttc ttgggaatac cggagtcaat
     8821 cattggactg atacaaaact cccggacaat aagaaggcag tttagaaaga gtctctcaag
     8881 aactttagaa gagtccttct acaactcaga gatccacggg attaatcgga taacccagac
     8941 acctcaaagg gtcggaaggg tgtggccttg ctcttcagag agggcagatc tacttaggga
     9001 gatctcttgg gggaggaaag tggtaggcac gacagttcct cacccttccg agatgttgga
     9061 gttgtttccc aaatcctcca tttcctgcac ttgtggagca acagggggag gcaatcctag
     9121 agtctctgta tcagtactcc cgtccttcga tcagtcattt ttctcacggg gccccctaaa
     9181 gggatacttg ggctcgtcca cctccatgtc aacccagcta ttccatgcat gggaaaaagt
     9241 cactaatgtt catgtggtga aaagggctct atcgttaaaa gaatctataa actggttcat
     9301 caataggaat tccaatttgg ctcaaactct aattggaaac atcatgtctc tgacaggccc
     9361 tgatttccct ctagaagagg cccctgtttt caaacggaca gggtcagcct tgcataggtt
     9421 caagtctgcc agatacagcg aaggagggta ttcttctgtt tgccctaacc ttctctccca
     9481 tatctctgtt agtacagaca ctatgtctga tttgacccaa aacgggaaga actatgattt
     9541 catgtttcag ccattgatgc tttatgcgca aacatggaca tcggaactgg tacagaggga
     9601 tacaagactt agagactcca cgtttcactg gcaccttcgg tgcaacagat gtgtgaggcc
     9661 cattgatgat ataacactgg aaacttctca gatcttcgag ttcccggatg tgtcaaaaag
     9721 gatatccagg atggtttctg gagctgtccc tcaatttcag aagcttcctg atatccgtct
     9781 aagaccaggt gattttgaat ccctaagtgg tagagaaaag tctcgccata tagggtcagc
     9841 tcaggggctc ttatactcaa tcttagtagc aattcacgac tcaggataca atgatgggac
     9901 catcttccct gtcaacatat acggcaaagt ttcccccaga gactatttga gagggcttgc
     9961 aagagggatc ttgatagggt cctcgatttg cttcttgaca cgaatgacaa atattaacat
    10021 taacagacct cttgaattga tctcaggggt aatttcctat attctcctga ggctggataa
    10081 tcatccctct ctgtatataa tgcttagaga accgtctctt agaggagaaa tattctctat
    10141 ccctcagaaa atccccgccg cttacccaac cactatgaga gaaggcaaca gatcgatttt
    10201 gtgttacctc caacacgtgc tacgctatga gcgagaggca atcacggcgt ccccggagaa
    10261 tgactggctg tggatcttct cagacttcag aagtgtgaaa atgacgtact tgaccctcat
    10321 tacctaccag tctcacctcc tactccagag ggttgagaga aacttgtcta agagtatgag
    10381 agctactctg cgacaaatga gttccttaat gaggcaagtg ctgggtgggc acggagaaga
    10441 taccttggag tcagacgatg acattcaacg attactaaaa gactctttgc gaaagacaag
    10501 gtgggtggat caagaggtgc gccatgcagc tagaaccatg aatggagatt acagccccga
    10561 caagaaagta tcccacaagg caggatgttc agaatgggtc tgctctgctc aacagattgc
    10621 cgtctccacc tcagccaacc cggcccctgt ctcagagctt gacattaggg ccctctctaa
    10681 gaggtttcaa aaccccttga tctcgggcct gagagtggtt cagtgggcaa ccggtgccca
    10741 ttataagctt aagcctattc tagatgatct aaatgttttc ccatctctct gtcttgtaat
    10801 tggagacggg tcagggggaa tatcaagggc agttctcaac atgtttccag attctaagct
    10861 tgtgttcaac agcctattgg aggtgaatga tctgatggct tccggaacac atccactgcc
    10921 tccttcagca atcatgagtg gaggagatga catcatctcc agagtgatag actttgactc
    10981 aatatgggag aaaccgtccg acctgaggaa ctcggccacc tggagatact tccagtcggt
    11041 tcaaaaacag gtcaacatgt cgtatgacct cattatttgt gatgcagaag ttactgatat
    11101 tgcatctatc aaccggataa ctctgttgat gtctgatttc gcattgtcta tagatggacc
    11161 actttatctg gtcttcaaaa cttacgggac tatgctagta aacccggact ataaagctat
    11221 tcaacatctg tcaagagcgt tcccttcggt cacagggttt gtaacccaag taacttcatc
    11281 cttttcttct gagctatacc tccggttctc taaacgagga aagtttttca gggacgccga
    11341 gtacttgacc tcttccaccc ttcgagagat gagccttgtg ttgttcaatt gcagcagccc
    11401 caaaagtgag atgcagagag ctcgttcctt aaactatcaa gacctggtaa ggggatttcc
    11461 tgaagagatc atatcaaatc cttacaacga aatgatcata actctgattg acaatgatgt
    11521 agagtccttc ctagtccaca agatggtgga tgatcttgag ctacagaggg gaactctgtc
    11581 taaagtggct atcattatat ccatcatgat cgttttttcc aatagagtct tcaacatttc
    11641 caaacctttg actgacccct tgttctaccc cccatctgat cctaaaatcc tgaggcactt
    11701 caacatatgt tgcagtacta tgatgtatct atctaccgct ttaggcgacg tccctaactt
    11761 cgcaagactt catgacctgt ataacagacc tataacttgt tacttcagaa aacaagttat
    11821 tcgagggaat atttatctat cttggagttg gtccgatgat accccagtgt tcaagagagt
    11881 agcctgtaat tctagcttga gtctgtcatc tcactggatc aggttgatct acaagatagt
    11941 gaagactacc agactcattg gcagcataaa agacctatca ggagaggtag aacgacatct
    12001 tcatgggtat aacagatgga tcaccctcga ggatatccga tctagatcat ccctactaga
    12061 ttacagttgt ttgtaagccg gatattaccg aaagcctgtg catgctaaaa ttcttgtatg
    12121 atgcatcttg aaaaaaacaa gatcttgaat ccggacctct ggttgtttga ttgttttttc
    12181 catctttatt gtttttttgt taagcgtggg tcggcatggc atctccacct cctcgcggtc
    12241 cgacctgggc atccgaagga ggacgcacgt ccactcggat ggctaaggga gggcggggat
    12301 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
    12361 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaagtcgcg
    12421 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    12481 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    12541 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    12601 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    12661 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    12721 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    12781 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    12841 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    12901 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    12961 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    13021 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    13081 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    13141 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    13201 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    13261 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    13321 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    13381 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    13441 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    13501 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    13561 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    13621 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    13681 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    13741 cccacccttg gaggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    13801 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    13861 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    13921 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    13981 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    14041 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    14101 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    14161 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    14221 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    14281 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    14341 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    14401 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    14461 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    14521 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    14581 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    14641 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    14701 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    14761 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    14821 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    14881 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    14941 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaaatgag ctgatttaac
    15001 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag
    15061 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    15121 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg cgagggtttt cccagtcacg
    15181 acgttgtaaa acgacggcca gtgagcgcgc ctagttatta atagtaatca attacggggt
    15241 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    15301 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    15361 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    15421 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
    15481 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
    15541 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
    15601 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
    15661 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
    15721 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
    15781 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
    15841 gggagaccca agctggctag attaagcgtc tgatgagtcc gtgaggacga aacccggcgt
    15901 accgggtc
//
