LOCUS       sequence-394142-        3450 bp    DNA     circular SYN 14-APR-2025
DEFINITION  express full length human PLD4 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Yuan M, Peng L, Huang D, Gavin A, Luan F, Tran J, Feng Z, Zhu X, 
            Matteson J, Wilson IA, Nemazee D
  TITLE     Structural and mechanistic insights into disease-associated 
            endolysosomal exonucleases PLD3 and PLD4.
  JOURNAL   Structure. 2024 Jun 6;32(6):766-779.e7. doi: 
            10.1016/j.str.2024.02.019. Epub 2024 Mar 26.
  PUBMED    38537643
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.str.2024.02.019"; journalName: "Structure"; date: 
            "2024-06-6- 6"; volume: "32"; issue: "6"; pages: "766-779.e7"
COMMENT     Sequence Label: SuExp-hPLD4
FEATURES             Location/Qualifiers
     source          1..3450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        75..454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        455..658
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     609..629
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     655..679
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    2258..2392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2282..2301)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2336..2355
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2435..3023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2515..2534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3048..3422)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(join(3423..3450,1..36))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttatcagg gttattgtct catgagcgga tacataacgc gtcgcgaggc catatgggtt
       61 aacccatggc cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      121 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      181 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      241 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      301 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      361 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      421 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      481 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      541 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
      601 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      661 tttagtgaac cgtcagatcg cctggagacg tcgagccgag tggttgtgcc tccatagaag
      721 ccaccatgct gaagcctctt tggaaagcag cagtggcccc cacatggcca tgctccatgc
      781 cgccccgccg cccgtgggac agagaggctg gcacgttgca ggtcctggga gcgctggctg
      841 tgctgtggct gggctccgtg gctcttatct gcctcctgtg gcaagtgccc cgtcctccca
      901 cctggggcca ggtgcagccc aaggacgtgc ccaggtcctg ggagcatggc tccagcccag
      961 cttgggagcc cctggaagca gaggccaggc agcagaggga ctcctgccag cttgtccttg
     1021 tggaaagcat cccccaggac ctgccatctg cagccggcag cccctctgcc cagcctctgg
     1081 gccaggcctg gctgcagctg ctggacactg cccaggagag cgtccacgtg gcttcatact
     1141 actggtccct cacagggcct gacatcgggg tcaacgactc gtcttcccag ctgggagagg
     1201 ctcttctgca gaagctgcag cagctgctgg gcaggaacat ttccctggct gtggccacca
     1261 gcagcccgac actggccagg acatccaccg acctgcaggt tctggctgcc cgaggtgccc
     1321 atgtacgaca ggtgcccatg gggcggctca ccaggggtgt tttgcactcc aaattctggg
     1381 ttgtggatgg acggcacata tacatgggca gtgccaacat ggactggcgg tctctgacgc
     1441 aggtgaagga gcttggcgct gtcatctata actgcagcca cctggcccaa gacctggaga
     1501 agaccttcca gacctactgg gtactggggg tgcccaaggc tgtcctcccc aaaacctggc
     1561 ctcagaactt ctcatctcac ttcaaccgtt tccagccctt ccacggcctc tttgatgggg
     1621 tgcccaccac tgcctacttc tcagcgtcgc caccagcact ctgtccccag ggccgcaccc
     1681 gggacctgga ggcgctgctg gcggtgatgg ggagcgccca ggagttcatc tatgcctccg
     1741 tgatggagta tttccccacc acgcgcttca gccacccccc gaggtactgg ccggtgctgg
     1801 acaacgcgct gcgggcggca gccttcggca agggcgtgcg cgtgcgcctg ctggtcggct
     1861 gcggactcaa cacggacccc accatgttcc cctacctgcg gtccctgcag gcgctcagca
     1921 accccgcggc caacgtctct gtggacgtga aagtcttcat cgtgccggtg gggaaccatt
     1981 ccaacatccc attcagcagg gtgaaccaca gcaagttcat ggtcacggag aaggcagcct
     2041 acataggcac ctccaactgg tcggaggatt acttcagcag cacggcgggg gtgggcttgg
     2101 tggtcaccca gagccctggc gcgcagcccg cgggggccac ggtgcaggag cagctgcggc
     2161 agctctttga gcgggactgg agttcgcgct acgccgtcgg cctggacgga caggctccgg
     2221 gccaggactg cgtttggcag ggctaaatct agaagctgat ccagacatga taagatacat
     2281 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2341 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttggatatcc
     2401 tcgagactag tgggcccgtt taaacacatg tgtttttcca taggctccgc ccccctgacg
     2461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     2881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2941 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3001 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgtca gtcctgctcc
     3061 tcggccacga agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac
     3121 ggctgctcgc cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg
     3181 acctccgacc actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg
     3241 ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc
     3301 acaccggcga agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac
     3361 tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc
     3421 atactcttcc tttttcaata ttattgaagc
//
