LOCUS       sequence-394497-        9453 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expression of human ERBB4 receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-ERBB4-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(51..70)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        242..621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        622..825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     776..796
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     870..889
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        870..888
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4958..4999
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5009..5026
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5049..5066)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5055..5279
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5325..5753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5412..5431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5622..5643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5762..5782)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5767..6096
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5947..6082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6009..6028
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6163..6957
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6217..6236)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6827..6846
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7133..7254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7170..7189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7224..7243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7303..7319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7303..7319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7316..7338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7327..7343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7351..7357,7358..7375,7376..7381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7396..7417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7534..7551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7705..8293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7785..7804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8464..9255,9256..9324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9087..9106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9325..9429)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
       61 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
      121 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
      181 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      241 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      601 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      661 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      721 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      781 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
      841 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
      901 ggctagttaa gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattgccctt
      961 caccatgaag ccggcgacag gactttgggt ctgggtgagc cttctcgtgg cggcggggac
     1021 cgtccagccc agcgattctc agtcagtgtg tgcaggaacg gagaataaac tgagctctct
     1081 ctctgacctg gaacagcagt accgagcctt gcgcaagtac tatgaaaact gtgaggttgt
     1141 catgggcaac ctggagataa ccagcattga gcacaaccgg gacctctcct tcctgcggtc
     1201 tgttcgagaa gtcacaggct acgtgttagt ggctcttaat cagtttcgtt acctgcctct
     1261 ggagaattta cgcattattc gtgggacaaa actttatgag gatcgatatg ccttggcaat
     1321 atttttaaac tacagaaaag atggaaactt tggacttcaa gaacttggat taaagaactt
     1381 gacagaaatc ctaaatggtg gagtctatgt agaccagaac aaattccttt gttatgcaga
     1441 caccattcat tggcaagata ttgttcggaa cccatggcct tccaacttga ctcttgtgtc
     1501 aacaaatggt agttcaggat gtggacgttg ccataagtcc tgtactggcc gttgctgggg
     1561 acccacagaa aatcattgcc agactttgac aaggacggtg tgtgcagaac aatgtgacgg
     1621 cagatgctac ggaccttacg tcagtgactg ctgccatcga gaatgtgctg gaggctgctc
     1681 aggacctaag gacacagact gctttgcctg catgaatttc aatgacagtg gagcatgtgt
     1741 tactcagtgt ccccaaacct ttgtctacaa tccaaccacc tttcaactgg agcacaattt
     1801 caatgcaaag tacacatatg gagcattctg tgtcaagaaa tgtccacata actttgtggt
     1861 agattccagt tcttgtgtgc gtgcctgccc tagttccaag atggaagtag aagaaaatgg
     1921 gattaaaatg tgtaaacctt gcactgacat ttgcccaaaa gcttgtgatg gcattggcac
     1981 aggatcattg atgtcagctc agactgtgga ttccagtaac attgacaaat tcataaactg
     2041 taccaagatc aatgggaatt tgatctttct agtcactggt attcatgggg acccttacaa
     2101 tgcaattgaa gccatagacc cagagaaact gaacgtcttt cggacagtca gagagataac
     2161 aggtttcctg aacatacagt catggccacc aaacatgact gacttcagtg ttttttctaa
     2221 cctggtgacc attggtggaa gagtactcta tagtggcctg tccttgctta tcctcaagca
     2281 acagggcatc acctctctac agttccagtc cctgaaggaa atcagcgcag gaaacatcta
     2341 tattactgac aacagcaacc tgtgttatta tcataccatt aactggacaa cactcttcag
     2401 cacaatcaac cagagaatag taatccggga caacagaaaa gctgaaaatt gtactgctga
     2461 aggaatggtg tgcaaccatc tgtgttccag tgatggctgt tggggacctg ggccagacca
     2521 atgtctgtcg tgtcgccgct tcagtagagg aaggatctgc atagagtctt gtaacctcta
     2581 tgatggtgaa tttcgggagt ttgagaatgg ctccatctgt gtggagtgtg acccccagtg
     2641 tgagaagatg gaagatggcc tcctcacatg ccatggaccg ggtcctgaca actgtacaaa
     2701 gtgctctcat tttaaagatg gcccaaactg tgtggaaaaa tgtccagatg gcttacaggg
     2761 ggcaaacagt ttcattttca agtatgctga tccagatcgg gagtgccacc catgccatcc
     2821 aaactgcacc caagggtgta acggtcccac tagtcatgac tgcatttact acccatggac
     2881 gggccattcc actttaccac aacatgctag aactcccctg attgcagctg gagtaattgg
     2941 tgggctcttc attctggtca ttgtgggtct gacatttgct gtttatgtta gaaggaagag
     3001 catcaaaaag aaaagagcct tgagaagatt cttggaaaca gagttggtgg aaccattaac
     3061 tcccagtggc acagcaccca atcaagctca acttcgtatt ttgaaagaaa ctgagctgaa
     3121 gagggtaaaa gtccttggct caggtgcttt tggaacggtt tataaaggta tttgggtacc
     3181 tgaaggagaa actgtgaaga ttcctgtggc tattaagatt cttaatgaga caactggtcc
     3241 caaggcaaat gtggagttca tggatgaagc tctgatcatg gcaagtatgg atcatccaca
     3301 cctagtccgg ttgctgggtg tgtgtctgag cccaaccatc cagctggtta ctcaacttat
     3361 gccccatggc tgcctgttgg agtatgtcca cgagcacaag gataacattg gatcacaact
     3421 gctgcttaac tggtgtgtcc agatagctaa gggaatgatg tacctggaag aaagacgact
     3481 cgttcatcgg gatttggcag cccgtaatgt cttagtgaaa tctccaaacc atgtgaaaat
     3541 cacagatttt gggctagcca gactcttgga aggagatgaa aaagagtaca atgctgatgg
     3601 aggaaagatg ccaattaaat ggatggctct ggagtgtata cattacagga aattcaccca
     3661 tcagagtgac gtttggagct atggagttac tatatgggaa ctgatgacct ttggaggaaa
     3721 accctatgat ggaattccaa cgcgagaaat ccctgattta ttagagaaag gagaacgttt
     3781 gcctcagcct cccatctgca ctattgacgt ttacatggtc atggtcaaat gttggatgat
     3841 tgatgctgac agtagaccta aatttaagga actggctgct gagttttcaa ggatggctcg
     3901 agaccctcaa agatacctag ttattcaggg tgatgatcgt atgaagcttc ccagtccaaa
     3961 tgacagcaag ttctttcaga atctcttgga tgaagaggat ttggaagata tgatggatgc
     4021 tgaggagtac ttggtccctc aggctttcaa catcccacct cccatctata cttccagagc
     4081 aagaattgac tcgaatagga gtgaaattgg acacagccct cctcctgcct acacccccat
     4141 gtcaggaaac cagtttgtat accgagatgg aggttttgct gctgaacaag gagtgtctgt
     4201 gccctacaga gccccaacta gcacaattcc agaagctcct gtggcacagg gtgctactgc
     4261 tgagattttt gatgactcct gctgtaatgg caccctacgc aagccagtgg caccccatgt
     4321 ccaagaggac agtagcaccc agaggtacag tgctgacccc accgtgtttg ccccagaacg
     4381 gagcccacga ggagagctgg atgaggaagg ttacatgact cctatgcgag acaaacccaa
     4441 acaagaatac ctgaatccag tggaggagaa cccttttgtt tctcggagaa aaaatggaga
     4501 ccttcaagca ttggataatc ccgaatatca caatgcatcc aatggtccac ccaaggccga
     4561 ggatgagtat gtgaatgagc cactgtacct caacaccttt gccaacacct tgggaaaagc
     4621 tgagtacctg aagaacaaca tactgtcaat gccagagaag gccaagaaag cgtttgacaa
     4681 ccctgactac tggaaccaca gcctgccacc tcggagcacc cttcagcacc cagactacct
     4741 gcaggagtac agcacaaaat atttttataa acagaatggg cggatccggc ctattgtggc
     4801 agagaatcct gaatacctct ctgagttctc cctgaagcca ggcactgtgc tgccgcctcc
     4861 accttacaga caccggaata ctgtggttgc aaagggcaat tctgcagata tccagcacag
     4921 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     4981 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
     5041 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5101 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5161 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5221 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     5281 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     5341 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5401 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5461 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5521 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5581 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5641 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5701 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5761 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5821 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5881 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5941 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6001 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     6061 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     6121 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6181 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6241 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6301 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6361 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6421 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6481 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6541 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6601 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6661 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     6721 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6781 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6841 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6901 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6961 ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     7021 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7081 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     7141 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7201 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7261 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     7321 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7381 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7441 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7501 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7561 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7621 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7681 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7741 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7801 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7861 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7921 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8041 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8101 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8161 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8221 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8281 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8341 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8401 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8461 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8521 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8581 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8641 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8701 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8761 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8821 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8881 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8941 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9001 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9061 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9121 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9181 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9241 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9301 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9361 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9421 ggttccgcgc acatttcccc gaaaagtgcc acc
//
