LOCUS       sequence-394814-        9906 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express SARS-CoV-2 5 UTR in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Tang Z, Hegde S, Hao S, Selvaraju M, Qiu J, Wang J
  TITLE     Chemical-guided SHAPE sequencing (cgSHAPE-seq) informs the binding 
            site of RNA-degrading chimeras targeting SARS-CoV-2 5' untranslated 
            region.
  JOURNAL   Nat Commun. 2025 Jan 8;16(1):483. doi: 10.1038/s41467-024-55608-w.
  PUBMED    39779694
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-01-8"; volume: "16(1):483. doi"; pages: " 
            10.1038/s41467-024-55608-w"
COMMENT     Sequence Label: pLV-SARS-COV-2-5UTR-GLuc
FEATURES             Location/Qualifiers
     source          1..9906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..245
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             246..426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    473..598
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1091..1324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1509..1553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1819..1936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1945..1965
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2026..2329
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2330..2533
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2484..2504
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2554..2578
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2880..3434
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="GVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRGK
                     LPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQ
                     GGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQR
                     CATFASKIQGQVDKIKGAGGD"
     protein_bind    complement(3435..3459)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(3460..4034,4035..4037,4038..4046)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3627..3644)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3854..3873
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4047..4712
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             4047..4712
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     protein_bind    complement(4731..4751)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    4764..5352
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4817..4837)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5235..5246)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5383..5882
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5418..5441)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5815..5834
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        5892..5939
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5958..6356
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6443..6676
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6748..6869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6785..6804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6839..6858
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6909..7044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7064..7083)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7065..7083)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7093..7110)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7093..7109)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7102..7124)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7251..7706
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7338..7357)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7548..7569
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7732..7836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7837..7905,7906..8697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8055..8074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8868..9456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9357..9376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9610..9627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9744..9765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9780..9785,9786..9803,9804..9810)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9818..9834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9823..9845
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9842..9858
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9842..9858
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9877..9897
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9879..9897
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
       61 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
      121 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
      181 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
      241 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      421 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
      481 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
      541 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
      601 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
      661 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
      721 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
      781 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
      841 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
      901 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
      961 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1021 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1081 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1141 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1201 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     1261 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     1321 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     1381 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     1441 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     1501 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     1561 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     1621 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     1681 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     1741 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     1801 ctcgacggta tcgctagctt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     1861 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     1921 aaaaattcaa aattttacta gtatcaactt tgtatagaaa agttgtagtt attaatagta
     1981 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2041 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2101 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2161 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2221 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2281 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2341 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2401 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2461 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2521 tataagcaga gctggtttag tgaaccgtca gatccaagtt tgtacaaaaa agcaggctgc
     2581 caccattaaa ggtttatacc ttcccaggta acaaaccaac caactttcga tctcttgtag
     2641 atctgttctc taaacgaact ttaaaatctg tgtggctgtc actcggctgc gtgcttagtg
     2701 cactcacgca gtataattaa taactaatta ctgtcgttga caggacacga gtaactcgtc
     2761 tatcttctgc aggctgctta cggtttcgtc cgtgttgcag ccgatcatca gcacatctag
     2821 gtttcgtccg ggtgtgaccg aaaggtaaga tggagagcct tgtccctggt ttcaacgagg
     2881 gagtcaaagt tctgtttgcc ctgatctgca tcgctgtggc cgaggccaag cccaccgaga
     2941 acaacgaaga cttcaacatc gtggccgtgg ccagcaactt cgcgaccacg gatctcgatg
     3001 ctgaccgcgg gaagttgccc ggcaagaagc tgccgctgga ggtgctcaaa gagatggaag
     3061 ccaatgcccg gaaagctggc tgcaccaggg gctgtctgat ctgcctgtcc cacatcaagt
     3121 gcacgcccaa gatgaagaag ttcatcccag gacgctgcca cacctacgaa ggcgacaaag
     3181 agtccgcaca gggcggcata ggcgaggcga tcgtcgacat tcctgagatt cctgggttca
     3241 aggacttgga gcccatggag cagttcatcg cacaggtcga tctgtgtgtg gactgcacaa
     3301 ctggctgcct caaagggctt gccaacgtgc agtgttctga cctgctcaag aagtggctgc
     3361 cgcaacgctg tgcgaccttt gccagcaaga tccagggcca ggtggacaag atcaaggggg
     3421 ccggtggtga ctaaacccag ctttcttgta caaagtgggc ccctctccct cccccccccc
     3481 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     3541 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     3601 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     3661 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3721 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     3781 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3841 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3901 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     3961 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     4021 aacacgatga taatatggcc acaaccatgc ccgccatgaa gatcgagtgc cgcatcaccg
     4081 gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccgagcagg
     4141 gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc
     4201 tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga
     4261 accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg
     4321 aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg
     4381 gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga
     4441 tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg
     4501 gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca
     4561 gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt
     4621 tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc
     4681 agcacgcctt caagaccccc atcgccttcg ccagatctcg agctcgatga caactttatt
     4741 atacatagtt gatcaattcc gataatcaac ctctggatta caaaatttgt gaaagattga
     4801 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4861 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4921 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4981 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5041 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5101 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     5161 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5221 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5281 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5341 ccgcctcccc gcatcgggaa ttcccgcggt tcgaattcta ccgggtaggg gaggcgcttt
     5401 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     5461 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     5521 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     5581 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     5641 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     5701 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     5761 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     5821 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     5881 cgacctcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     5941 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     6001 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     6061 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     6121 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     6181 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     6241 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     6301 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac
     6361 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6421 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6481 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6661 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6721 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6781 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6841 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6901 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6961 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7021 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     7081 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7141 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7201 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     7261 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7321 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7381 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7441 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7501 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7561 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7621 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     7681 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     7741 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7801 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7861 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7921 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7981 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8041 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8101 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8161 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8221 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8281 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8341 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8401 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8461 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8521 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8581 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8641 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8701 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8761 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8821 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8881 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8941 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9001 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9061 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagagag aaaggcggac
     9301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9541 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9601 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9661 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     9721 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9781 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9841 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     9901 caaaag
//
