LOCUS       sequence-396069-       10603 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1940
FEATURES             Location/Qualifiers
     source          1..10603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6460..7048
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6513..6533)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6931..6942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7120..7353
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7431..7552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7468..7487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7522..7541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7592..7727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7747..7766)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7748..7766)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7776..7793)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7776..7792)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7785..7807)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7934..8389
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8021..8040)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8231..8252
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8415..8519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8520..8588,8589..9380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8738..8757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9551..10139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10040..10059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10293..10310
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10427..10448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10463..10468,10469..10486,10487..10493)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10501..10517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10506..10528
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10525..10541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10525..10541
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10560..10580
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10562..10580
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gtgatgaacc tgatggagca
     4141 gcctatcaag gtgactgagt ggcagcagac atacacctac gactcgggta tccactcggg
     4201 cgccaacacc tgcgtgccct ccgtcagcag caagggcatc atggaggagg atgaggcctg
     4261 cgggcgccag tacacgctca agaaaaccac cacttacacc cagggggtgc cccccagcca
     4321 aggtgatctg gagtaccaga tgtccacaac agccagggcc aaacgggtgc gggaggccat
     4381 gtgccctggt gtgtcaggcg aggacagctc gcttctgctg gccacccagg tggaggggca
     4441 ggccaccaac ctgcagcgac tggccgagcc gtcccagctg ctcaagtcgg ccattgtgca
     4501 tctcatcaac taccaggacg atgccgagct ggccactcgc gccctgcccg agctcaccaa
     4561 actgctcaac gacgaggacc cggtggtggt gaccaaggcg gccatgattg tgaaccagct
     4621 gtcgaagaag gaggcgtcgc ggcgggccct gatgggctcg ccccagctgg tggccgctgt
     4681 cgtgcgtacc atgcagaata ccagcgacct ggacacagcc cgctgcacca ccagcatcct
     4741 gcacaacctc tcccaccacc gggaggggct gctcgccatc ttcaagtcgg gtggcatccc
     4801 tgctctggtc cgcatgctca gctcccctgt ggagtcggtc ctgttctatg ccatcaccac
     4861 gctgcacaac ctgctcctgt accaggaggg cgccaagatg gccgtgcgcc tggccgacgg
     4921 gctgcaaaag atggtgcccc tgctcaacaa gaacaacccc aagttcctgg ccatcaccac
     4981 cgactgcctg cagctcctgg cctacggcaa ccaggagagc aagctgatca tcctggccaa
     5041 tggtgggccc caggccctcg tgcagatcat gcgtaactac agttatgaaa agctgctctg
     5101 gaccaccagt cgtgtgctca aggtgctatc cgtgtgtccc agcaataagc ctgccattgt
     5161 ggaggctggt gggatgcagg ccctgggcaa gcacctgacc agcaacagcc cccgcctggt
     5221 gcagaactgc ctgtggaccc tgcgcaacct ctcagatgtg gccaccaagc aggagggcct
     5281 ggagagtgtg ctgaagattc tggtgaatca gctgagtgtg gatgacgtca acgtcctcac
     5341 ctgtgccacg ggcacactct ccaacctgac atgcaacaac agcaagaaca agacgctggt
     5401 gacacagaac agcggtgtgg aggctctcat ccatgccatc ctgcgtgctg gtgacaagga
     5461 cgacatcacg gagcctgccg tctgcgctct gcgccacctc actagccgcc accctgaggc
     5521 cgagatggcc cagaactctg tgcgtctcaa ctatggcatc ccagccatcg tgaagctgct
     5581 caaccagccc aaccagtggc cactggtcaa ggcaaccatc ggcttgatca ggaatctggc
     5641 cctgtgccca gccaaccatg ccccgctgca ggaggcagcg gtcatccccc gcctcgtcca
     5701 actgctggtg aaggcccacc aggatgccca gcgccacgta gctgcaggca cacagcagcc
     5761 ctacacggat ggtgtgagga tggaggagat tgtggagggc tgcaccggag cactgcacat
     5821 cctcgcccgg gaccccatga accgcatgga gatcttccgg ctcaacacca ttcccctgtt
     5881 tgtgcagctc ctgtactcgt cggtggagaa catccagcgc gtggctgccg gggtgctgtg
     5941 tgagctggcc caggacaagg aggcggccga cgccattgat gcagaggggg cctcggcccc
     6001 actcatggag ttgctgcact cccgcaacga gggcactgcc acctacgctg ctgccgtcct
     6061 gttccgcatc tccgaggaca agaacccaga ctaccggaag cgcgtgtccg tggagctcac
     6121 caactccctc ttcaagcatg acccggctgc ctgggaggct gcccagagca tgattcccat
     6181 caatgagccc tatggagatg acatggatgc cacctaccgc cccatgtact ccagcgatgt
     6241 gccccttgac ccgctggaga tgcacatgga catggatgga gactacccca tcgacaccta
     6301 cagcgacggc ctcaggcccc cgtaccccac tgcagaccac atgctggcct agtaatgaac
     6361 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     6421 gtgcacctcg acatctagtt atacacgcgt taagtcgaca atcaacctct ggattacaaa
     6481 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6541 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6601 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6661 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6721 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6781 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6841 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     6901 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6961 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7021 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     7081 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7141 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7201 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7261 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7321 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     7381 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     7441 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7501 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7561 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7621 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7681 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     7741 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     7801 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     7861 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     7921 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7981 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8041 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8101 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8161 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8221 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8281 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8341 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     8401 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8461 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8521 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8581 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8641 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8701 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8761 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8821 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8881 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8941 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9001 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9061 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9121 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9181 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9241 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9301 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9361 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9421 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9661 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10201 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10261 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10321 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    10381 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    10441 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10501 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    10561 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
