LOCUS       sequence-396164-       11224 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1235
FEATURES             Location/Qualifiers
     source          1..11224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7081..7669
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7134..7154)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7552..7563)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7741..7974
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8052..8173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8089..8108)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8143..8162
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8213..8348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8368..8387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8369..8387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8397..8414)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8397..8413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8406..8428)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8555..9010
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8642..8661)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8852..8873
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9036..9140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9141..9209,9210..10001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9359..9378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10172..10760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10661..10680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10914..10931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11048..11069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11084..11089,11090..11107,11108..11114)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11122..11138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11127..11149
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11146..11162
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11146..11162
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11181..11201
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11183..11201
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaag ttcaaaagga ggcaccaagc
     4141 atttccgtcc ttcttcaaga tgaaggtaag ccttcccagt gatccaagct gtgttgaaga
     4201 aatcttgcgg gaatcgcagc atctgacccc aggattcacc ttacaaaagt ggaatgaccc
     4261 aaccaccaga gcttctacaa agtcagtgtc tttcaaatcg atgcttgagg atgacctgaa
     4321 gctgagcagt gatgaagatg accttgagcc tgtgaagacc ttgaccactc agtgcactgc
     4381 cactgagctc taccaggctg ttgaaaaggc aaaacctagg aataatcctg tgaacccacc
     4441 cttggccact ccccagcccc cacctgcagt gcaagccagc gggggttctg gcagctccag
     4501 cgaatcggag agcagctctg agtcggattc agacactgaa agtagcacca ctgacagcga
     4561 atctaatgag gcacctcgtg tggcaactcc agagcctgag ccaccctcaa ccaacaagtg
     4621 gcaactggat aaatggctta acaaagtgac atcccagaac aagtctttta tttgtggcca
     4681 aaatgaaaca cccatggaga ctatttctct gcctcctcca atcatccaac caatggaagt
     4741 ccagatgaaa gtgaagacga atgccagtca ggtcccagct gaacccaaag aaaggcctct
     4801 cctcagtctc attagggaga aagcccgtcc acggcccact cagaaaattc cagaaacaaa
     4861 ggctttgaag cataagttgt caacaactag tgagacagtg tctcaaagga caattgggaa
     4921 aaaacagccc aaaaaagttg agaagaacac cagcactgac gagtttacct ggcccaaacc
     4981 aaatattacc agcagcactc ccaaagaaaa agaaagtgtg gagcttcatg acccaccaag
     5041 aggccgcaac aaagccactg cccacaaacc agcccctagg aaagaaccaa gacctaacat
     5101 ccctttggct cccgagaaga agaagtacag agggcctggc aagattgtgc caaagtctcg
     5161 ggaattcatt gaaacagatt catctacatc tgactccaac acagatcagg aagagaccct
     5221 gcaaatcaaa gtcctgcctc cgtgcattat ttctggaggt aatactgcca aatccaagga
     5281 aatctgtggt gccagcctga ccctcagcac cttaatgagt agcagtggca gcaacaacaa
     5341 cttatccatc agtaatgaag agccaacatt ttcacctatt cctgtcatgc aaactgaaat
     5401 cctgtcccct ctgcgagatc atgagaacct gaaaaacctc tgggtgaaga ttgaccttga
     5461 cttactctct agagtacctg gccacagctc actccatgca gcacctgcca agccagacca
     5521 caaggagact gccacaaaac ccaagcgtca gacagctgtc acagctgtgg agaaaccagc
     5581 ccctaagggc aaacgtaagc acaagccaat agaagttgca gagaagatcc ctgagaagaa
     5641 gcagcgcctg gaggaggcca caactatctg cttgctccct ccttgcatct caccagcccc
     5701 accccacaag cctcccaaca ctagagaaaa taattcatcc aggagagcaa atagaagaaa
     5761 ggaagaaaaa ctatttcctc ctccactttc cccactgcca gaggaccctc cacgccgcag
     5821 aaatgtcagt ggcaataatg gtccctttgg tcaagacaaa aacatcgcca tgactggaca
     5881 aatcacatct accaaaccta agagaactga aggcaaattc tgtgctactt tcaaagggat
     5941 atcggtaaat gagggagaca ctccaaaaaa ggcatcctct gccaccatca ctgtcaccaa
     6001 tactgctatt gccactgcta ctgtcactgc tactgccatt gtcaccacca ctgtcacagc
     6061 tactgccacc gccacggcca ccaccacaac tactaccact accatttcca ccatcacctc
     6121 taccatcact actggcctca tggatagcag tcacctggag atgacgtcct gggcggctct
     6181 gccccttcta tccagcagca gcactaatgt ccggagaccc aagctcactt ttgatgactc
     6241 ggttcacaat gctgattatt acatgcaaga agctaagaag ctgaagcaca aagctgatgc
     6301 actgttcgag aaatttggca aagctgtgaa ttatgctgat gccgccctct ccttcactga
     6361 atgtggcaat gccatggaac gcgaccctct ggaagcaaag tccccataca ccatgtactc
     6421 tgagactgtg gagctcctca ggtatgcaat gaggctgaag aactttgcaa gtcccttggc
     6481 ttcggatggg gacaaaaagc tagcagtact atgctaccga tgtttatcac tcctctattt
     6541 gagaatgttt aagctgaaga aggaccatgc tatgaagtac tccagatcac tgatggaata
     6601 ttttaagcaa aatgcttcaa aagtcgcaca gataccctct ccatgggtaa gcaatggaaa
     6661 gaacactcca tccccagtgt ctctcaacaa cgtctccccc atcaacgcaa tggggaactg
     6721 taacaatggc ccagtcacca ttccccagcg cattcaccac atggctgcca gccacgtcaa
     6781 catcactagc aatgtgttac ggggctatga acactgggat atggccgaca aactgacaag
     6841 agaaaacaaa gaattctttg gtgatctgga cacgctgatg gggcctctga cccagcacag
     6901 cagcatgacc aatcttgtcc gctacgttcg ccaaggactg tgttggctgc gcatcgatgc
     6961 ccacttgttg tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     7021 tatatcttgt ggaaaggacg atcaatgaag tcgaccgtca ccgccacgcg ttaagtcgac
     7081 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7141 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7201 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7261 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7321 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7381 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7441 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7501 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7561 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7621 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     7681 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7741 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7801 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7861 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7921 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     7981 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8041 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8101 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8161 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     8221 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8281 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8341 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     8401 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8461 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8521 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     8581 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8641 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8701 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8761 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8821 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8881 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8941 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     9001 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9061 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9121 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9181 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9241 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9301 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     9361 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     9421 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9481 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9541 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9601 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9661 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9721 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9781 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9841 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9901 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9961 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10021 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10081 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10141 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10561 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10741 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10801 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10861 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10921 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    10981 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    11041 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    11101 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    11161 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    11221 aagc
//
