LOCUS       sequence-396175-       12121 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1230
FEATURES             Location/Qualifiers
     source          1..12121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4467..4478)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      4791..4800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7978..8566
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8031..8051)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8449..8460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8638..8871
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8949..9070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8986..9005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9040..9059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9110..9245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9265..9284)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9266..9284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9294..9311)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9294..9310)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9303..9325)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9452..9907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9539..9558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9749..9770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9933..10037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10038..10106,10107..10898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10256..10275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11069..11657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11558..11577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11811..11828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11945..11966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11981..11986,11987..12004,12005..12011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12019..12035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12024..12046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12043..12059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12043..12059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12078..12098
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12080..12098
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac agcttcgact tagccctgct
     4141 ccaggaatgg gacctcgagt cactgtgggg tgaagacatc ttaaaccaga ggaatgactc
     4201 tctagttgtg gaatttcagt catcagccag cagatgcagg agtgtctatg aaccagatag
     4261 aaatgcatta cggaggaaag aacgagaaag aagaaatcaa gaaactcaac aggatgatgg
     4321 cacgtttaat tctagttact ctctcttcag tgagccctac aagactaaca agggggatga
     4381 actctccaac cggatccaga acactttagg caattatgat gaaatgaaag actttttaac
     4441 tgatagatcc aatcagagtc atctcgttgg agttcccaaa cctggggttc ctcagactcc
     4501 tgtgaacaag atcgatgaac attttgttgc agattcaaga gcccagaacc agccctcgtc
     4561 tatctgtagc actacaactt ccacaccagc agctgtcccc gtgcagcaga gtaagagagg
     4621 cactatgggc tggcagaagg ctgggcaccc accctctgac ggccaacaga gagcaacaca
     4681 acagggctct ctcaggacct tgcttggaga tggtgttggc agacagcagc ctcgggccaa
     4741 acaagtgtgc aatgtggagg tgggccttca gacccaggag aggccacctg ccatggcggc
     4801 caagcacagc agcagcggac actgtgttca gaactttcct ccatccctag cttcaaaacc
     4861 cagcctggtc cagcagaaac cgaccgcgta tgtgaggcca atggacggcc aagatcaggc
     4921 ccctgatgag tctcctaagc tgaagtcgtc ttcggaaacc agcgtgcact gcacatcata
     4981 caggggagtc cctgccagca agccggagcc tgccagagcc aaggccaagc tctccaagtt
     5041 cagcatcccc aagcaggggg aggagagtag atctggagaa accaacagct gtgttgaaga
     5101 aataatccgg gagatgacct ggcttccacc actttctgct attcaagcac ctggcaaagt
     5161 ggaaccaacc aaatttccat ttccaaataa ggactctcag cttgtatcct ctggacacaa
     5221 taatccaaag aaaggtgatg cagagccaga gagtccagac aatggcacat cgaatacatc
     5281 aatgctggaa gatgacctta agctaagcag tgatgaagag gagaatgaac agcaggcagc
     5341 tcagagaacg gctctccgcg ctctctctga cagcgccgtg gtccagcagc ccaactgcag
     5401 aacctcggtg ccttccagca agggcagcag cagcagcagc agcagcggca gcagcagctc
     5461 ctccagcgac tcagagagca gctccggatc tgactcggag accgagagca gctccagcga
     5521 gagtgagggc agcaagcccc cccacttctc cagccccgag gctgaaccgg catcctctaa
     5581 caagtggcag ctggataaat ggctaaacaa agttaatccc cacaagcctc ctattctgat
     5641 ccaaaatgaa agccacgggt cagagagcaa tcagtactac aacccggtga aagaggacgt
     5701 ccaggactgt gggaaagtcc ccgacgtttg ccagcccagc ctgagagaga aggagatcaa
     5761 gagcacttgc aaggaggagc aaaggccaag gacagccaac aaggcccctg ggagtaaagg
     5821 cgtgaagcag aagtccccgc ccgcggccgt ggccgtggcg gtgagcgcag ccgccccgcc
     5881 acccgcagtg ccctgtgcgc ccgcggagaa cgcgcccgcg cctgcccgga ggtccgcggg
     5941 caagaagccc accaggcgca ccgagaggac ctcagccggg gacggcgcca actgccaccg
     6001 gcccgaggag cccgcggccg cggacgcgct ggggacgagc gtggtggtcc ccccggagcc
     6061 caccaaaacc aggccctgtg gcaacaacag agcgagccac cgcaaggagc tgcgctcctc
     6121 cgtgacctgc gagaagcgcc gcacgcgggg gctaagcagg atcgtcccca aatccaagga
     6181 gttcattgag acagagtcgt catcttcatc ctcctcctcg gactccgacc tggagtccga
     6241 gcaggaggag taccctctgt ccaaagcaca gaccgtggct gcctctgcct cctccgggaa
     6301 tgatcagagg ctgaaggagg ccgctgccaa cgggggcagt ggtcctaggg cccctgtagg
     6361 ctccatcaac gccaggacca ccagtgacat cgccaaggag ctggaggagc agttctacac
     6421 actggtcccc tttggccgga acgaacttct ctcccctcta aaggacagtg atgagatcag
     6481 gtctctctgg gtcaaaatcg acctgaccct cctgtccagg atcccagaac acctgcccca
     6541 ggagccaggg gtattgagcg cccctgccac caaggactct gagagcgcac cgcccagcca
     6601 cacctcggac acacctgcag aaaaggcttt gccaaaatcc aagaggaaac gcaagtgtga
     6661 caacgaagac gactacaggg agatcaagaa gtcccaggga gagaaagaca gctcttcaag
     6721 actggccacc tccaccagta atactttgtc tgcaaaccac tgcaacatga acatcaacag
     6781 tgtggcaata ccaataaata aaaatgaaaa aatgcttcgg tcgcccatct cacccctctc
     6841 tgatgcatct aaacacaaat acaccagcga ggacttaact tcttccagcc gacctaatgg
     6901 caacagtttg tttacttcag cctcttccag caaaaagcct aaggccgaca gccagctgca
     6961 gcctcacggc ggagacctca cgaaagcagc tcacaacaat tctgaaaaca ttcccctcca
     7021 caagtcacgg ccgcagacga agccgtggtc tccaggctcc aacggccaca gggactgcaa
     7081 gaggcagaaa cttgtcttcg atgatatgcc tcgcagtgcc gattatttta tgcaagaagc
     7141 taaacgaatg aagcataaag cagatgcaat ggtggaaaag tttggaaagg ctttgaacta
     7201 tgctgaagca gcattgtcgt ttatcgagtg tggaaatgca atggaacaag gccccatgga
     7261 atccaaatct ccttatacga tgtattcaga aacagtagag ctcatcaggt atgctatgag
     7321 actaaaaacc cactcaggcc ccaatgccac accagaagac aaacaactgg ctgcattatg
     7381 ttaccgatgc ctggccctcc tgtactggcg gatgtttcga ctcaaaaggg accacgctgt
     7441 aaagtattca aaagcactaa tcgactattt caagaactca tctaaagccg cccaagcccc
     7501 atctccgtgg ggggccagtg gaaagagcac tggaacccca tcccccatgt ctcccaaccc
     7561 ctctcccgcc agctccgtgg ggtctcaggg cagcctctcc aacgccagcg ccctgtcccc
     7621 gtcgaccatc gtcagcatcc cacagcgcat ccaccagatg gcggccaacc acgtcagcat
     7681 caccaacagc atcctgcaca gctacgacta ctgggagatg gccgacaacc tggccaagga
     7741 aaaccgagaa ttcttcaacg acctggatct gctcatgggg ccggtcaccc tgcacagcag
     7801 catggagcac ctggtccagt actcccaaca gggcctgcac tggctgcgga acagcgccca
     7861 cctgtcatag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     7921 atcttgtgga aaggacgatg gttcgagtca ggctagagtt caacgcgtta agtcgacaat
     7981 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8041 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8101 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8161 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8221 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8281 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8341 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8401 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8461 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8521 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     8581 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8641 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8701 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8761 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8821 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     8881 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     8941 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9001 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9061 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     9121 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9181 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9241 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     9301 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9361 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9421 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     9481 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9541 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9601 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9661 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9721 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9781 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9841 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     9901 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9961 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10021 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10081 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10141 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10201 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10261 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10321 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10381 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10441 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10501 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10561 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10621 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10681 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10741 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10801 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10861 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10921 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    11581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11761 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11821 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    11881 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    11941 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    12001 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    12061 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    12121 c
//
