LOCUS       sequence-396260-       14077 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1606
FEATURES             Location/Qualifiers
     source          1..14077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5135..5146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    9934..10522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9987..10007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10405..10416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10594..10827
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10905..11026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10942..10961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10996..11015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11066..11201
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11221..11240)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11222..11240)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11250..11267)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11250..11266)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11259..11281)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11408..11863
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11495..11514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11705..11726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11889..11993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11994..12062,12063..12854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12212..12231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13025..13613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13514..13533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13767..13784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13901..13922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13937..13942,13943..13960,13961..13967)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13975..13991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13980..14002
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13999..14015
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13999..14015
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14034..14054
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14036..14054
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gcaaacgacc attttaactt
     4141 tactggcctt ccccctgcac ctgctgcctc aggactgaaa ccctctcctt cctcagggga
     4201 gggcctctac actaacgggt ctcccatgaa cttcccccag caagggaaaa gtttgaatgg
     4261 ggatgtgaat gttaatggct tatctactgt atctcacact actacttcag ggattttgaa
     4321 ctctgctccc cactcctcca gcacctcaca cctccatcac cccagcgtgg cctacgactg
     4381 tctctggaac tactcacagt acccatctgc caatcctggc agcaacctca aggacccacc
     4441 ccttctctcc cagttctcgg ggggacaata cccactcaac ggcatccttg ggggcagccg
     4501 gcaaccttca tccccaagtc ataacactaa ccttcgggct gggagccaag agttctgggc
     4561 caacggtacc cagagtccca tggggcttaa ctttgattca caagaactgt atgattcctt
     4621 tcctgaccag aattttgagg tgatgcccaa tggaccccct agttttttca cctccccaca
     4681 gacttctcct atgttgggat ctagcattca aacctttgca ccctcccagg aggtaggcag
     4741 tggtatccat cctgatgagg cagcagaaaa ggagatgact tcagttgtgg cagagaatgg
     4801 cactggcttg gtaggcagct tggagctgga agaagagcag ccagaactga agatgtgtgg
     4861 ctacaatggc tctgtccctt ctgtggaatc gttacaccaa gaggtctcag tcctggtccc
     4921 tgaccccaca gtgagctgtt tagatgatcc ttcacatctt cctgatcaac tggaagacac
     4981 tccaatcctc agtgaagact ctctggagcc cttcaactct ctggcaccag agccagtgag
     5041 tggaggacta tatggtattg atgacacgga gctgatgggt gcagaggaca agctgcctct
     5101 tgaggacagc cctgtgattt ctgcccttga ttgcccttcc ctcaataatg ctactgcctt
     5161 cagtctcctg gcagatgata gtcaaacatc aacctctatc tttgccagtc ccacctctcc
     5221 acctgtccta ggggagtctg tcctgcaaga taacagcttt gacctgaata atggtagtga
     5281 cgctgaacag gaagaaatgg aaactcaatc ttcagacttc ccaccatccc tgacccagcc
     5341 agctcctgat cagtcatcca ctattcagct acatccagca acctcaccag cagtctcgcc
     5401 aacaacctcc ccagcagtct ccctagtggt ttctccagca gcctccccag aaatctctcc
     5461 agaagtttgt cccgcagctt ctacagttgt ctctccagca gtcttctcag tggtctctcc
     5521 agcttcctca gcagtcctcc cagcagtctc cttagaagtc ccgttgacgg cttcagtgac
     5581 atccccaaaa gcctctcccg taacttcccc agcagctgcc tttccaacag cctccccagc
     5641 aaataaggat gtcagcagct ttctagaaac cactgctgac gtggaagaga tcactggaga
     5701 aggactcact gcttctggta gtggtgatgt catgaggaga cgtattgcta ccccagaaga
     5761 agttcgtctt cccctccaac atgggtggcg gagagaggtg cgcatcaaga agggcagcca
     5821 ccgatggcag ggggagacct ggtattatgg cccctgtggg aagaggatga agcaatttcc
     5881 agaagtgatc aagtacctga gccgcaacgt ggtacacagt gtccgccgag agcacttcag
     5941 cttcagtccc cgtatgcctg ttggagattt ctttgaagaa agagacacgc cagagggctt
     6001 gcagtgggtg cagctctcag cagaggagat cccgtcgagg attcaggcaa ttactggcaa
     6061 acggggtcga cctcgaaaca ctgagaaggc taagactaag gaagtcccca aggtgaaacg
     6121 gggtcgaggt cggccaccta aggtcaaaat cactgagcta ttgaacaaga cagacaaccg
     6181 ccccctaaag aaactggagg cccaagaaac attgaatgag gaggataaag caaagattgc
     6241 taaaagcaag aagaagatga ggcagaaggt tcaacgggga gagtgtcaga ctactatcca
     6301 agggcaggcc agaaataagc ggaaacaaga gaccaagagc ttaaagcaga aggaagctaa
     6361 gaagaaatcc aaggctgaga aagaaaaagg aaagacaaag caggaaaaac tgaaggaaaa
     6421 agtcaagagg gaaaagaagg agaaggtaaa aatgaaggaa aaggaggagg tgaccaaagc
     6481 caagccagcc tgtaaagcag ataagaccct ggccacacag aggcgcttgg aggaacggca
     6541 gaggcagcag atgatcttgg aggaaatgaa gaagccgaca gaggatatgt gtctgactga
     6601 ccaccagccc ctgcctgact tctcacgagt ccctggtctg acattgccca gtggagcctt
     6661 ctcagactgc ttgaccattg tggagttcct gcatagcttt ggcaaggtgc tgggctttga
     6721 tcctgccaaa gatgtgccta gcctgggggt cctgcaggag ggactcctgt gtcaaggtga
     6781 cagcttgggt gaggtgcaag acctgctggt caggctgctg aaggctgcac tccatgatcc
     6841 tggctttccc tcctactgtc agtccctaaa gatcttgggg gagaaggtgt ctgagatccc
     6901 actgacaaga gacaatgtgt cagagatcct gcgctgcttc cttatggcat atggagtaga
     6961 gccagccctc tgtgaccgcc tgcgcaccca gccttttcag gcccagccac cccagcagaa
     7021 ggctgctgtc ctggccttcc ttgtgcatga gctcaatggc tccaccctca tcatcaatga
     7081 gattgacaag actctggaga gtatgtccag ctacaggaaa aacaagtgga ttgttgaagg
     7141 ccggctccgg aggctgaaaa ctgttctggc caagcgaact gggcggtctg aagtagagat
     7201 ggaagggcca gaggaatgcc tgggacggag gcgcagttct cggatcatgg aggagaccag
     7261 tggcatggaa gaagaggaag aagaggagtc tatagcagct gtccctggcc gcaggggtcg
     7321 aagagatgga gaggttgatg ccacagcatc tagcatccca gagctagagc gccagataga
     7381 aaaactcagc aagcgtcagc ttttctttcg caaaaagctg cttcactcat cccagatgct
     7441 tcgggcggtc tccctgggtc aggaccgcta cagacgtcgc tactgggtat tgccgtattt
     7501 ggctggtatc tttgtagaag gaacagaggg gaacttagtt cctgaggagg tgataaagaa
     7561 ggaaactgac tccttaaaag tggcagccca tgcgtcactc aaccctgccc tcttctctat
     7621 gaagatggag ttagctggct ccaacaccac tgccagttct cctgcccggg cccgaggccg
     7681 acctcgaaaa actaagcccg ggtctatgca acctaggcat cttaagtccc ctgtcagggg
     7741 tcaggattca gaacagcccc aggcccagct tcagcctgag gctcagcttc atgctcctgc
     7801 ccagccccag cctcagcttc agcttcagct tcagtcccat aaggggttcc tggagcaaga
     7861 aggctcccct ttgtcactgg gtcagagcca gcatgacctc agccagtcag ccttcctgtc
     7921 ttggctgagc cagactcaga gccatagctc cctgttgagc agctcagtcc tcacacctga
     7981 tagcagtccg ggaaaactag acccagctcc atcacaaccc ccggaggagc cagagcctga
     8041 tgaggcagaa tccagccctg atcctcaagc actctggttt aacatctcag cccagatgcc
     8101 ctgcaatgct gcccccacac cgccccctgc agtttctgag gaccaaccca ctccctcccc
     8161 tcagcagctt gcctcctcca agccaatgaa tagacctagt gctgccaacc cttgttctcc
     8221 agtgcagttc tcttccacgc ccttggctgg gttggcccct aagaggcgag caggagaccc
     8281 tggagaaatg ccacagagtc ccacagggct gggacagccc aaacggagag ggagacctcc
     8341 cagtaagttc ttcaaacaga tggaacagcg ttacctaacc cagctgacag cccagcctgt
     8401 cccacctgag atgtgctcag gctggtggtg gatacgagat cctgagatgt tggatgccat
     8461 gctcaaggcc ctacaccccc gaggtatccg ggagaaggca cttcacaaac accttaacaa
     8521 gcacagggac ttcttgcagg aagtctgcct gcggccctca gctgacccca tctttgagcc
     8581 caggcaacta cctgcctttc aagaagggat tatgagctgg tcccccaaag agaagacata
     8641 cgagacagac ctagcagtgc ttcaatgggt agaggagctg gagcagcggg ttatcatgtc
     8701 tgatctgcag attcggggct ggacatgtcc tagcccagac tctacccgtg aagacttggc
     8761 ctactgtgag cacctctccg actcccagga ggatatcacc tggcgaggtc ggggcaggga
     8821 gggactggca cctcagcgta aaactaccaa ccctttggac ctggctgtga tgcggctggc
     8881 tgccctggaa cagaatgtag aacggcggta cctgcgggag cccctctggc caactcatga
     8941 ggttgtgctg gagaaggccc tgcttagcac acctaatggt gcccctgagg gcaccactac
     9001 agagatatca tatgagatca cccctcgcat tcgtgtctgg cgccagaccc tcgagcggtg
     9061 ccggagcgca gcccaggtgt gcttgtgcct gggccagctg gagaggtcca ttgcctggga
     9121 gaagtctgtc aacaaagtga catgtctagt ctgccggaag ggtgacaatg atgagtttct
     9181 tctgctttgt gatgggtgtg accgtggctg ccacatttac tgccatcgtc ccaagatgga
     9241 ggctgtccca gaaggagatt ggttctgtac tgtctgtttg gctcagcagg tggagggaga
     9301 attcactcag aagcctggtt tcccaaagcg tggccagaag cggaaaagtg gttattcgct
     9361 gaacttctca gagggtgatg gccgccgacg ccgggtactg ttgaggggcc gagaaagccc
     9421 agcagcaggg cctcggtact cggaagaagg gctctccccc tccaagcggc ggcgactctc
     9481 tatgcggaac caccacagtg atctcacatt ttgcgagatt atcctgatgg agatggagtc
     9541 ccatgatgca gcctggcctt tcctagagcc tgtgaaccca cgtttggtga gtgggtaccg
     9601 gcgcatcatc aaaaatccta tggatttttc caccatgcgg gagcggctgc tcaggggagg
     9661 gtacaccagc tcagaggagt ttgcggctga tgccctcctg gtatttgaca actgccagac
     9721 tttcaacgag gatgactctg aagtaggcaa ggctgggcac atcatgcgcc gcttcttcga
     9781 gagccgctgg gaggagtttt atcagggaaa acaggccaat ctgtagtaat gaactagtcc
     9841 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaatgatg
     9901 actgtcattc cgcgagtaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     9961 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
    10021 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
    10081 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
    10141 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
    10201 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
    10261 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
    10321 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
    10381 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    10441 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
    10501 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
    10561 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
    10621 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10681 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10741 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10801 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
    10861 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
    10921 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10981 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
    11041 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    11101 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    11161 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
    11221 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
    11281 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
    11341 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    11401 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    11461 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    11521 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    11581 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    11641 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    11701 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    11761 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    11821 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
    11881 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    11941 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    12001 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    12061 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    12121 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    12181 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    12241 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    12301 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    12361 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    12421 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    12481 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    12541 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    12601 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    12661 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    12721 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    12781 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    12841 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    12901 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    12961 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    13021 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    13081 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    13141 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    13201 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    13261 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    13321 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    13381 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    13441 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    13501 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    13561 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    13621 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    13681 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    13741 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    13801 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    13861 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    13921 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    13981 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    14041 accctcacta aagggaacaa aagctggagc tgcaagc
//
