LOCUS       sequence-396321-        9651 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes recombinant mouse VEGF164 when coupled with amber stop codon
            suppression by non-canonical amino acid incorporation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Miller LN, Walters AE, Denninger JK, Hanson MA, Marshall AH, 
            Johantges AC, Hosawi M, Sebring G, Rieskamp JD, Ding T, Rindani R, 
            Chen KS, Goldberg ME, Senthilvelan S, Volk A, Zhao F, Askwith C, 
            Wester JC, Kirby ED
  TITLE     Neural stem and progenitor cells support and protect adult 
            hippocampal function via vascular endothelial growth factor 
            secretion.
  JOURNAL   Mol Psychiatry. 2024 Nov 11. doi: 10.1038/s41380-024-02827-8.
  PUBMED    39528687
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Psychiatry. 2024 Nov 11. doi: 10.1038/s41380-024-02827-8."
COMMENT     Sequence Label: VEGF164TAG
FEATURES             Location/Qualifiers
     source          1..9651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..152
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        311..1489
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          542..1480
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1437..1457
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1506..1524
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2117..2134
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2157..2745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2210..2230)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2628..2639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2778..3002
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    3044..3161
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3210..3720
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3639..3657
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3742..4341
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3742..3761)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4238..4258
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4469..4702
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4774..4895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4811..4830)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4865..4884
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4935..5070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5091..5109)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5119..5136)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5119..5135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5128..5150)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5277..5732
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5364..5383)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5574..5595
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5758..5862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5863..5931,5932..6723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6081..6100)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6894..7482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7383..7402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7636..7653
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7770..7791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7806..7811,7812..7829,7830..7836)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7844..7860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7849..7871
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7868..7884
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7868..7884
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7903..7923
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7905..7923
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7951..8177
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8178..8358
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8405..8530
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9023..9256
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9441..9485
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
       61 ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg
      121 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      181 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
      241 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      301 tggactatca ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
      361 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
      421 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
      481 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
      541 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
      601 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
      661 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
      721 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
      781 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
      841 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
      901 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
      961 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     1021 tctggtgcct ggtctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     1081 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     1141 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     1201 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     1261 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     1321 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     1381 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     1441 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt
     1501 ggtactaata cgactcacta tagggagacc caagctggct agcatgaact ttctgctctc
     1561 ttgggtgcac tggaccctgg ctttactgct gtacctccac catgccaagt ggtcccaggc
     1621 tgcacccacg acagaaggag agtagcagaa gtcccatgaa gtgatcaagt tcatggatgt
     1681 ctaccagcga agctactgcc gtccgattga gaccctggtg gacatcttcc aggagtaccc
     1741 cgacgagata gagtacatct tcaagccgtc ctgtgtgccg ctgatgcgct gtgcaggctg
     1801 ctgtaacgat gaagccctgg agtgcgtgcc cacgtcagag agcaacatca ccatgcagat
     1861 catgcggatc aaacctcacc aaagccagca cataggagag atgagcttcc tacagcacag
     1921 cagatgtgaa tgcagaccaa agaaagacag aacaaagcca gaaaatcact gtgagccttg
     1981 ttcagagcgg agaaagcatt tgtttgtcca agatccgcag acgtgtaaat gttcctgcaa
     2041 aaacacagac tcgcgttgca aggcgaggca gcttgagtta aacgaacgta cttgcagatg
     2101 tgacaagcca aggcggcatc atcatcatca tcattaacga tatcaagctt atcgataatc
     2161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2521 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     2581 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc
     2761 tgctcgagag atctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2821 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2881 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2941 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3001 gggaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     3061 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     3121 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     3181 gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc
     3241 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
     3301 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
     3361 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
     3421 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
     3481 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
     3541 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
     3601 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
     3661 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag
     3721 ggggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     3781 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     3841 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     3901 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     3961 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     4021 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     4081 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     4141 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     4201 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     4261 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     4321 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     4381 gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     4441 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     4501 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4561 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4621 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4681 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     4741 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     4801 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4861 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     4921 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5041 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     5101 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5161 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5221 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     5281 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5341 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5401 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5461 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5521 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5581 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5641 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5701 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     5761 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5821 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5881 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5941 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6001 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6061 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6121 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6181 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6241 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6301 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6361 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6421 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6481 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6541 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6601 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6661 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6721 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6781 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6841 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6901 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6961 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7021 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7081 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7141 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7201 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7261 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7321 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7381 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7441 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7501 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7561 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7621 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7681 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7741 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7801 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7861 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     7921 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     7981 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     8041 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     8101 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     8161 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8221 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8281 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8341 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     8401 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     8461 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     8521 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     8581 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     8641 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     8701 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     8761 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     8821 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     8881 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     8941 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     9001 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     9061 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     9121 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     9181 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     9241 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     9301 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     9361 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     9421 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     9481 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     9541 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     9601 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg a
//
