LOCUS       sequence-396330-       10846 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression in mammalian cells or oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Abderemane-Ali F, Rossen ND, Kobiela ME, Craig RA, Garrison CE, Chen
            Z, Colleran CM, O'Connell LA, Du Bois J, Dumbacher JP, Minor DL
  TITLE     Evidence that toxin resistance in poison birds and frogs is not 
            rooted in sodium channel mutations and may rely on ""toxin sponge"" 
            proteins.
  JOURNAL   J Gen Physiol. 2021 Sep 6;153(9):e202112872. doi: 
            10.1085/jgp.202112872. Epub 2021 Aug 5.
  PUBMED    34351379
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol."; date: "2021-09-6"; volume: "153(9):e202112872. doi"; 
            pages: " 10.1085/jgp.202112872. Epub 2021 Aug 5"
COMMENT     Sequence Label: Pt NaV1.4
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6437..6454)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6443..6667
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6713..7141
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6800..6819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7010..7031
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7150..7170)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7155..7484
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7335..7470
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7397..7416
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7551..8345
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7605..7624)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8215..8234
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8519..8640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8556..8575)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8610..8629
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8689..8705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8689..8705)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8702..8724)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8713..8729
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8737..8743,8744..8761,8762..8767))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8782..8803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8920..8937)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9091..9679)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9171..9190)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9850..10641,10642..10710))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10473..10492
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10711..10815)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagatggctg ggaagattca tccacccgga cctgattcac tgcagagatt
      961 cactaaagaa actatcgcta agattgagaa aactatcgaa gaggaggaga tccgcaaggc
     1021 caagcagatc gaggtgctgg acgaggtggt gcggaagcct aacaatgggc tggaggcagg
     1081 caagagcctg ccactgatct acggcgatcc ccctccagag ctgatcggcg tgcctctgga
     1141 ggatctggac ccattctatt ccaaccagaa gacctttatc gtgctgaata agtctaaggc
     1201 cgtgttccgg ttcaccgcca caaaggccat gtacctgctg gaccccttca accctctgag
     1261 gacaggcgcc atcaaggtgc tgatccactc catcttctct ttctttatca tgatcacaat
     1321 cctggccaac tgcgtgttta tgaccatgaa tgatccccct ccctgggcca agaatgtgga
     1381 gtacaccttc acaggcatct atacctttga gagcctgatc aagatcttcg ccagaggctt
     1441 ttgcatcgac agcttcacct tcctgcggga cccctggaac tggctcgatt tctgcgtgat
     1501 cgtgatggcc tacaccacag agtttgtgga tctgggcaat gtgagcgccc tgcggacctt
     1561 cagagtgctg agggccctga agaccatcac agtgatcccc ggcctgaaga caatcgtggg
     1621 cgccctgatc cagtccgtga agaagctggc cgacgtgatg atcctgaccg tgttctgcct
     1681 ggccgtgttt gccctgatcg gcctgcagct gttcatgggc aacctgcgcc agaagtgcgt
     1741 gcggtggcca ccttccgagg aggcctacga gtactatcgc aacgagacaa tcaattctat
     1801 ggacttcgac gataacagca ccctgtacaa caacaccgat atgttcacaa acatctccat
     1861 cgactggagc acctacctga acgatgagtc taatttctat tttctggatg gcgccctgga
     1921 cgcactgctg tgcggcaaca gctccgactc cggcaagtgc cccgagtctt acacctgtat
     1981 caagacaggc cggaacccta attatggcta caccaacttc gactccttta attgggccta
     2041 cctggccctg ttccgcctga tgacacagga ttattgggag aatctgtttc agctgacact
     2101 gcgggcagca ggcaagacct atatgatctt cttcgtgctg gtcatcttcc tgggcgcctt
     2161 ttacctggtg aacctgatcc tggccgtggt ggctatggcc tacgacgagc agaatgaggc
     2221 caccatccag gaggccctgg agaaggagaa ggagttccag gatatgctgg agcagctgaa
     2281 gaagcaccag gaggagcagg gacagaggaa ggccagcatg gcctccgtgg gcctggtgaa
     2341 cgaggacatc aagaagagca ccgagctgga tctggccgag gaggacaagg agaagatgga
     2401 ttgtaatggc agagtgatcc cccgggtggt catcgagaca atcgacgccg tggaggacga
     2461 tgccacctct gtgaagacag atctggagaa gaacagcaag atgtacctgg aggagcccac
     2521 cctgcagaag agaacaggct ctagcgtgtc tgtggtgagc gaggccatga tcgaggagct
     2581 ggaggaggcc cagcagacat gcccaccctg gtggtataag ttcgccaaca cctttctgaa
     2641 gtgggactgt tgtaagccct ggattcagtt caaggagatc gtgaggctgg tggtcatgga
     2701 ccccttcgtg gacctgggca tcacaatttg tatcgtgctg aataccctgt tcatggctat
     2761 ggagcactac cctatgaccg aggagtttga gaacgtgctg agcgtgggca atctggtgtt
     2821 cacaggcatc tttgccgccg agatgttcct gaagctgatc gccctggacc catactatta
     2881 cttccaagtg ggctggaacg tgtttgatag catcatcgtg accctgtccc tggtggagct
     2941 gggcctggct aatgtggagg gcctgagcgt gctgcgctcc ttccggctgc tgagagtgtt
     3001 taagctggcc aagtcttggc ctaccctgaa catgctgatc aagatcatcg gcaacagcgt
     3061 gggcgccctg ggcaatctga cactggtgct ggccatcatc gtgttcatct ttgccgtggt
     3121 gggcatgcag ctgttcggca agagctacaa ggagtgcgtg tgcaagatca attccgactg
     3181 cgtgctgcca agatggcaca tgcacgattt ctttcacagc ttcctgatcg tgttcagaat
     3241 cctgtgcggc gagtggatcg agacaatgtg ggactgcatg gaggtggcag gacaggcaat
     3301 gtgcctcaca gtgttcctga tggtcatggt cgtgggcaac ctggtggtgc tgaatctgtt
     3361 cctggccctg ctgctgtcct ctttttccgc cgacaacctg tctgccagcg acgatgacgg
     3421 cgagatgaac aatctgcaga tcgccatcgg caggatcaca cgcggcatcg atttcatcaa
     3481 gaagaccatg atctccctgc tgcccttcaa gaagaaggag cagaacaagg aggaagacaa
     3541 gaacaatatc gaggatgtgc gggagtcttt cgccctgaat cacctggcca ccgaggagga
     3601 gcctaagcca gagtgccccg ccatcgacgg ctttatcaag aagcctcgct acatcatgac
     3661 agagctggat cacatcaacc tgatcaacaa tccacactac cggatcgagg tgcccatcgc
     3721 ctccgaggag tctgatctgg actacctgga gacagacgag ctgagcacac agtccgagtt
     3781 cgaagagaca gtgatcaagc ctaagaagac cttctttgac ggcacatcta tgtgcagcac
     3841 cgccgattac aagcctccag agccagagga ggaggaggag acactggagc acaaggagga
     3901 ggatgaccct gaggagtgct tcacagaggc ctgcgtgcgg cggttcccat gcctgtatgt
     3961 ggacatcaag acagagaagg gcaagctgtg gtggaacgtg cgcaagacct gtttcaagat
     4021 cgtggagcac aattggttcg agacattcat catctttatg atcctgctga gctccggcgc
     4081 cctggccttt gaggacatct acatcgagca gcggcaggtc atcaagatca tcctggagta
     4141 cgccgataag gtgttcagct acgtgttcgt gatcgagatg ctgctgaaat gggtggccta
     4201 cggcttcaag gtgtacttca ccaacgcctg gtgttggctg gacttcctga tcgtggacgt
     4261 gagcctgatc agcctgaccg ccaattggct gggctattcc gagctgggcc caatcaagtc
     4321 tctgcggaca ctgagagccc tgaggcccct gcgcgccctg tccagatttg aaggcatgag
     4381 ggtggtggtg aacgcactgc tgggagcaat cccttccatt atgaatgtgc tgctggtgtg
     4441 cctgatcttc tggctgatct tttctatcat gggcgtgaac atgttcgccg gcaagtatta
     4501 cagatgcatc aataccacaa cctctgagct gtttgacatc agcgtggtga acaatagatc
     4561 cgattgtatg gccctgaact tcaccgagca ggtgaggtgg gtgaatgtga aggtgaactt
     4621 tgacaatgtg ggcctgggct acctgagcct gctgcaggtg gccacattca agggctggat
     4681 ggacatcatg tacgccgccg tggactccag gaagatcgag gatcagcccg tgtatgagat
     4741 caacctgtat atgtacctgt attttgtgat cttcatcatc tttggcgcct tctttaccct
     4801 gaatctgttc atcggcgtga tcatcgacaa ctttaatcag cagaagaaga agttcggcgg
     4861 caaggacatc ttcatgacag aggagcagaa gaagtattac aacgccatga agaagctggg
     4921 ctctaagaag ccagtgaagc ccatccctag accagagaat cccatccagg gcgtggtgtt
     4981 cgactttatc agcaagcagg ccttcgacat cttcatcatg atcctgatct gcctgaacat
     5041 gatcacaatg atgatcgaga cagatgacca gagcgacgag aaggtgaaca tcctgttcca
     5101 gatcaatctg gtgttcatcg tgatctttac aaccgagtgt ttcctgaaga tgtttgccct
     5161 gcgctattac ttctttaccg tgggctggaa tatcttcgac tttgtcgtgg tcatcatcag
     5221 catcgccggc atcgtgctgt ccgatctgat cgccgagtac ttcgtgtccc ccaccctgtt
     5281 cagagtgatc agactggcca ggatcggacg cgtgctgcgg ctgatcaagg gagccaaggg
     5341 catccggaca ctgctgttcg ccctgatgat gtctctgcct gccctgttca acatcggcct
     5401 gctgctgttc ctggtcatgt tcatcttttc tatcttcggc atgagcaatt ttgcttatgt
     5461 gaaaaaggag agcggcatcg atgacatgtt caactttgag acattcggca acagcatcat
     5521 ctgcctgttt gagatcacaa ccagcgccgg atgggacggc ctgctgaacc ccatcctgaa
     5581 tagcggcccc cctgattgtg acccacacat cgagaaccca ggaaccgcag tgcagggaaa
     5641 ctgcggcaat cctgccatcg gcatcgtgtt cttttgttcc tacatcatca tctctttcct
     5701 gatcgtgatc aatatgtata tcgccatcat cctggagaat tttaatgtgg caaccgagga
     5761 gtccggagag ccactgtgcg aggatgactt cgagatgttt tacgagacat gggagaagtt
     5821 cgatcccgac gccacccagt ttatcgccta tagccgcatg tccgatttcg tggacaccct
     5881 gcaggacccc ctgagaatcc agaagcctaa caagatcaag ctgatcacac tggatctgcc
     5941 tatggtggtg ggcgacaaga tccactgcct ggacatcctg ttcgccctga ccaaggaggt
     6001 gctgggcgac tccggcgaga tggatgccct gaaggcctct atggaggaga agtttatggc
     6061 cgccaaccct tctaaggtga gctacgagcc aatcacaacc acactgaaga ggaagcagga
     6121 ggaggtgtgc gccatcaaga tccagagggc cttcaggcgc cacctgctga agcggagcgt
     6181 gaagcacgcc tcctacatct atagacactc taaggatttt gacagcgccg tggaggaccc
     6241 accagagaag gagggcctgg tggccgatag gatcaacatc gcctacggcc agtatctgga
     6301 tgacgagggc gccaacggct cacagagacc cagaagcact aatagatttt caagaagcag
     6361 cggcgacgac taataagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
     6421 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6481 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6541 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6601 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6661 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6721 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6781 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6841 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6901 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6961 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7021 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7081 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7141 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7201 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7321 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7381 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7441 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7501 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     7561 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7621 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7681 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     7741 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7801 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7861 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7921 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7981 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     8041 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     8101 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     8161 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     8221 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     8281 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     8341 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     8401 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     8461 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     8521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8641 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     8701 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8761 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8821 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8881 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8941 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9001 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9061 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9121 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9181 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9241 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9301 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9361 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9421 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9481 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9541 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9601 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9721 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9781 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9841 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9901 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9961 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10021 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10081 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10141 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10201 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10261 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10321 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10381 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10441 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10501 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10561 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10621 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10681 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10741 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
