LOCUS       sequence-396782-        4663 bp    DNA     circular SYN 15-APR-2025
DEFINITION  gfp BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH60 - [ASELp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1916..1932)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1916..1932)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1929..1951)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2104..2692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2184..2203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2809..2833)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2933..3724,3725..3793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3556..3575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3794..3898)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3997..4019
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4011..4028
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4012..4028
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4652..4663,1..22))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcatacatt atacgaagtt atgatatcaa aaatgattac gccaagctgt aagtttaaac
       61 atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc
      121 actcacaacg atggatacgc taatgcccct agaaatgaga agtgcatcct aaacacatcc
      181 gatgcatccc agacaattcg taattttctt tctgactaat gaagctgatt aatacgcgta
      241 taggactgaa caaatatatt atctctgact tgttcgtcga agcccttcaa caagtttgct
      301 catgattatg agccgcctgg aatatatccg aaaactaata aaaactaaag aaaacaagat
      361 agaatattgt ttttataaca ctttttgtat atattaaaac ctcagaagac tgcccactta
      421 ttcattttca taactccttt ccaaaaaaga gaccaaaaat ggggccctct aagggcgaag
      481 aattgttcac gggagtagtc ccaatcttag tcgagcttga cggaggtgag ttttattatc
      541 gattttcggg attttctact tgtaattacc ttttgttttt aataagttgc ttttttccag
      601 atgttaatgg ccacaagttt tccgtgtctg gagaaggaga aggcgacgct acctacggta
      661 agctgaccct gaagttcatt tgcactactg gcaagttacc agtaccatgg ccgaccctgg
      721 tgacaacatt ttgctacggg gttcaatgct tctccagata cccaggtagg ttttcagttt
      781 caatttttgt tgcaactacc tcaactctat gttttcagat catatgaaaa gacacgactt
      841 ttttaaatcc gccatgccag agggttacgt tcaagaacgt accatttttt ttaaggacga
      901 tggtaactac aagacacgtg cggaggttaa atttgaaggc gatactctcg tgaaccgtat
      961 tgagctcaag gtaaattttt caagcttcta aattccaaaa taaaccattt taatttctta
     1021 attccaggga atcgatttca aggaagatgg aaacattttg ggccacaagc ttgaatataa
     1081 ttataatagt cataatgtct acattatggc cgacaaacaa aaaaacggta ttaaggttaa
     1141 cttcaagatt cgccacaata tcgaagatgg atctgtccaa cttgctgacc actaccaaca
     1201 aaatactcca attggagatg gaccggtttt gcttccagat aaccactacc tttccactca
     1261 gtctgccctc tctaaggatc cgaatgaaaa acgtgaccat atggttcttt tggagttcgt
     1321 taccgcagca ggaattactc acggaatgga tgaactgtac aagcccgggt gatggtctct
     1381 gagctatgca aaatcctttc aagcattccc ttcttctcta tcactcttct ttctttttgt
     1441 caaaaaattc tctcgctaat ttatttgctt ttttaatgtt attattttat gactttttat
     1501 agtcactgaa aagtttgcat ctgagtgaag tgaatgctat caaaatgtga ttctgatatc
     1561 caggaaacgg agaatctgtg cggcaatgtc ttatcatttg aaacgcgccg tatcgatgct
     1621 gacatcatgc ggctcccgtg ttagtcgaga agatgggtac gggcctttat tcatccaaca
     1681 ctctacggct tcttcaagag ctagtagggc accctgtagt tggaaaggga ttatttcgta
     1741 tggcgagccc ataccgtctt tcttgcggaa gcttaacacg ataggaagtt ggatagtttc
     1801 gaacgatggt tccatacgga acgctgtctc aaggatgagt ctgacggagt gtaactcgaa
     1861 ggctaggtgg aggctcagtg atgataagtc tgcgatggtg gatgcatgtg tcatggtcat
     1921 agctgtttcc tgtgtgaaat tgttatccgc tcagagggca caatcctatt ccgcgctatc
     1981 cgacaatctc caagacatta ggtggagttc agttcggcgt atggcatatg tcgctggaaa
     2041 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2101 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2161 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2221 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2281 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2341 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2401 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2461 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2521 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2581 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2641 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2701 tttgatcttt tctacggggt ctgacgctct attcaacaaa gccgccgtcc cgtcaagtca
     2761 gcgtaaatgg gtagggggct tcaaatcgtc ctcgtgatac caattcggag cctgcttttt
     2821 tgtacaaact tgttgataat ggcaattcaa ggatcttcac ctagatcctt ttaaattaaa
     2881 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2941 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3001 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3061 caatgatacc gcgagagcca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3121 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3181 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3241 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3301 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3361 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3421 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3481 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3541 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3601 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3661 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3721 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3781 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3841 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3901 catttccccg aaaagtgcca gatacctgaa acaaaaccca tcgtacggcc aaggaagtct
     3961 ccaataactg tgatccacca caagcgccag ggttttccca gtcacgacgt tgtaaaacga
     4021 cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc cgcctgacct
     4081 aagtgctttt cgagatcgcc aaaccggaga acgatcagga gaatgatgag tcgccacagg
     4141 agtcggcaag atacgtgcgt tatagatttg ctccaaactt tttgaaactc aaaacggtcg
     4201 gagcaactgt ggaattcaaa gtaggagaca tcccaatcca tcatttccga atgatcgaac
     4261 gtcacttctt caaagatcgc cttctgaagt gttttgactt tgaattcgga ttctgtattc
     4321 cgaattcacg aaacaactgt gaacatatct atgagttccc tcaactctct caacaactca
     4381 gtgagtcatt attctaaaaa gtacaattca aaagactaat ctctttttca gtggacgaca
     4441 tgatcaacaa tccaaacgag actcgttctg acagcttcta tttcgtcgat aacaaactcg
     4501 tcatgcacaa caaagccgac tactcatatg atgcataaat cttcattgtt gagtttatct
     4561 tgttgatttt tgaataaatt atcaactctt tactttttaa tgggttatga aataaataaa
     4621 cattgaaaac tgataaacaa cgttcatctc tataacttcg tat
//
