LOCUS       sequence-396874-       12624 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses HA-tagged human NLGN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12624)
  TITLE     Plasmids for expression of human NLGN1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SV40-puro-EF1a-HA-NLGN1-bc
FEATURES             Location/Qualifiers
     source          1..12624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(170..961,962..1030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     793..812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1031..1135)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1210..1229)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1401..1780
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1782..1980
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1935..1955
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1998..2178
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2225..2350
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2843..3076
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3261..3305
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3603..3720
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     rep_origin      3817..3952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3971..4570
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3971..3990)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4467..4487
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        4687..5865
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4918..5856
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5813..5833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5872..5881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6019..6045
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      6447..6456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8479..9067
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8532..8552)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8950..8961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9070..9086)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9071..9087)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9592..9772
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9798..9815)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9804..10028
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10074..10502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10161..10180)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10371..10392
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10511..10531)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10516..10845
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10696..10831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10893..10940
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10959..11333
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11463..11584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11500..11519)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11554..11573
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11633..11649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11633..11649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11646..11668)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11657..11673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11681..11687,11688..11705,11706..11711))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11726..11747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11864..11881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12035..12623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12115..12134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1141 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     1201 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     1261 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     1321 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     1381 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     1441 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     1501 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     1561 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     1621 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     1681 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     1741 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     1801 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     1861 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     1921 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     1981 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     2041 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2101 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     2161 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     2221 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     2281 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     2341 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     2401 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     2461 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     2521 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     2581 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     2641 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     2701 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     2761 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     2821 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     2881 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     2941 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     3001 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     3061 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     3121 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     3181 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     3241 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     3301 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     3361 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     3421 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     3481 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     3541 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
     3601 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     3661 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     3721 cgggtttatt acagggacag cagagatcca gtttggttaa ttagctagct gcatctcaat
     3781 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3841 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3901 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggctac
     3961 gcgtgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4021 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4081 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4141 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     4201 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4261 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4321 gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa
     4381 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4441 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4501 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4561 cggtgcctga gaccttctag tcttgaaagg agtgggaatt ggatcctgca aagatggata
     4621 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
     4681 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4741 ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg
     4801 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4861 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     4921 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     4981 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     5041 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     5101 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     5161 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     5221 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     5281 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     5341 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     5401 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     5461 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     5521 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     5581 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     5641 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     5701 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     5761 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     5821 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgactcga ggccaccatg
     5881 gcacttccca gatgcatgtg gccaaattat gtttggagag ctatgatggc atgtgtggtc
     5941 cacaggggtt ccggtgcccc attgactctc tgcttgttgg gatgtttgct acagactttt
     6001 cacgtactct ctcaaaagta tccatacgac gttccggact acgcaactag tttggatgat
     6061 gtggacccac tggtggctac caactttgga aagataagag ggattaagaa ggaactcaat
     6121 aatgaaattt tggggcctgt tattcaattt cttggggttc catatgcagc cccaccaaca
     6181 ggggaacgtc gttttcagcc tccagaacca ccatctccct ggtcagatat cagaaatgcc
     6241 actcaatttg ctcctgtgtg tccccagaat atcattgatg gcagattgcc agaagtcatg
     6301 cttcctgtgt ggtttactaa taacttggat gtggtttcat catatgtgca agaccagagc
     6361 gaagactgcc tatatttaaa tatatatgtc ccgactgagg atgatattcg ggacagtggg
     6421 ggtcccaaac cagtgatggt gtatatccat ggtggctcat atatggaagg tactggaaat
     6481 ttatatgatg gaagtgtctt ggcaagttat ggcaatgtga tcgtcatcac agtcaactat
     6541 cgacttggag tactcggttt cttgagtaca ggcgatcagg ctgcaaaggg gaactatgga
     6601 ctccttgatc tcatacaagc tttaagatgg actagtgaaa acattggatt ctttggtggt
     6661 gaccccttaa gaatcactgt ttttggatct ggtgctgggg gttcatgtgt caacctgctg
     6721 actttatccc attattctga aggtaaccgt tggagcaatt caaccaaagg actttttcaa
     6781 cgagcaatag ctcaaagtgg aacagccctt tccagctggg ctgttagttt tcaacctgca
     6841 aaatatgcta gaatgttggc cacaaaagtt ggttgcaatg tttcagatac agtagagtta
     6901 gtggaatgcc tacagaagaa gccttacaaa gaacttgttg accaagatat tcaaccagct
     6961 cgataccaca tagcctttgg acctgtgatt gatggtgatg taataccaga cgacccccag
     7021 atattgatgg agcaaggaga gtttctcaac tatgatataa tgttaggagt gaaccaaggg
     7081 gaagggttaa aatttgttga aaatatagta gatagcgatg atggtatatc agctagtgat
     7141 tttgactttg ctgtttcaaa ttttgttgat aatttatatg gatatcctga aggcaaagat
     7201 gttttgagag aaaccattaa gttcatgtat actgactggg ctgaccgtca taaccctgaa
     7261 accagaagaa agacattact ggctttgttt acggaccatc agtgggtggc accagctgta
     7321 gccacagcgg atcttcactc aaactttggt tcacctacgt acttctatgc cttttaccat
     7381 cattgccaaa cagatcaggt tccagcttgg gctgatgcag cccacggaga cgaggttccc
     7441 tatgtactgg gaatccccat gattggccct acagagttat ttccttgcaa tttctccaaa
     7501 aatgatgtga tgctgagtgc agttgtaatg acatactgga caaattttgc taaaactggt
     7561 gacccaaatc aaccagtccc tcaagacacg aaattcattc ataccaaacc caaccgtttt
     7621 gaagaagtag catggaccag atattcccag aaagaccaac tttatctcca tattggatta
     7681 aaaccaagag ttaaagaaca ttacagagcc aataaggtga acctctggtt ggagttggta
     7741 cctcatctgc ataatctcaa tgacatttct cagtatacct ctacaacaac taaagtgcca
     7801 tcaactgaca tcactttcag acctacgaga aaaaattctg tacctgtcac gtcagccttt
     7861 cccactgcca agcaggatga tcccaaacaa caaccaagtc cattttcagt ggatcaaagg
     7921 gactactcaa cagagctgag tgtcactatt gcagttggag catcactgct gtttctgaac
     7981 atcttggcct ttgcagccct gtactacaaa aaggataaga ggagacatga tgttcacagg
     8041 agatgcagcc ctcagcgcac tactaccaat gatctaaccc atgcacaaga agaggaaatc
     8101 atgtccctcc aaatgaagca cactgatttg gatcatgaat gtgagtccat tcatccacat
     8161 gaggtggttc ttcggaccgc ctgtccccca gattacacac tagctatgag gaggtcacct
     8221 gatgatgttc ccttaatgac acccaacacc attacaatga ttcccaacac tataccaggg
     8281 attcagccct tacacacatt caatacattt actggaggac agaacaatac tctgccccat
     8341 ccccatcccc acccccattc acattcaaca accagggtat gagctagctg ttcaatcaac
     8401 attccctccc gacctggact ggctattcat tcgccctttg ggtaagcaca cgtcgaattc
     8461 gatatcaagc ttatcggtaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8521 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8581 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8641 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8701 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8761 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8821 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8881 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8941 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     9001 cggcctcttc cgcgtcatcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     9061 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     9121 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     9181 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     9241 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgatga
     9301 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     9361 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     9421 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     9481 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     9541 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     9601 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9661 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9721 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     9781 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     9841 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     9901 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     9961 gcaggacagc aagggggagg attgggatga caatagcagg catgctgggg atgcggtggg
    10021 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
    10081 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
    10141 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
    10201 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
    10261 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
    10321 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
    10381 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
    10441 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
    10501 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
    10561 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
    10621 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
    10681 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    10741 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
    10801 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
    10861 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
    10921 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
    10981 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
    11041 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
    11101 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
    11161 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
    11221 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
    11281 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
    11341 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    11401 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    11461 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    11521 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    11581 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    11641 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    11701 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    11761 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    11821 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    11881 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    11941 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    12001 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    12061 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    12121 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    12181 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    12241 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    12301 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    12361 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    12421 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    12481 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    12541 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    12601 cgcgcagaaa aaaaggatct caag
//
