LOCUS       sequence-397910-       15706 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15706)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1269
FEATURES             Location/Qualifiers
     source          1..15706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    11563..12151
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11616..11636)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12034..12045)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12223..12456
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12534..12655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12571..12590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12625..12644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12695..12830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12850..12869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12851..12869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12879..12896)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12879..12895)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12888..12910)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13037..13492
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13124..13143)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13334..13355
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13518..13622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13623..13691,13692..14483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13841..13860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14654..15242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15143..15162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15396..15413
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15530..15551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15566..15571,15572..15589,15590..15596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15604..15620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15609..15631
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15628..15644
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15628..15644
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15663..15683
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15665..15683
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac actggggaca cagctctagg
     4141 acaaaaagct acctcaaggt ctggagaaac tgataaagca tcaggtagat ggagacagga
     4201 acaatcagct gttattaaga tgagcacttt tggcagtcat gaaggacagc ggcaaccaca
     4261 aatagagcct gagcaaatcg gaaacacagc atcagcacaa ctgtttggtt ctgggaaact
     4321 ggcctcccct agtgaagtgg tgcagcaagt cgcagagaag caatatccac cgcatcgtcc
     4381 gagtccttac tcatgccaac actcactctc tttccctcag cactcattgc cacagggggt
     4441 catgcacagc accaagccac atcagagcct cgaaggtcct ccgtggcttt tccctggccc
     4501 tttgccatcc gttgcctctg aggacttatt tccttttcct atacatggcc acagtggtgg
     4561 ttatcctaga aaaaagattt caagtctgaa ccctgcttat agccaatact cccagaaaag
     4621 tattgaacag gcagaagagg ctcacaagaa agagcacaaa cccaaaaagc ctggcaagta
     4681 catttgccct tactgcagca gagcgtgtgc caaacctagt gtactgaaaa aacacatcag
     4741 gtcccatact ggggagcggc catatccatg tataccttgt ggtttctctt tcaagacaaa
     4801 gagcaatttg tacaagcaca ggaagtcaca tgcccatgca attaaggcag gattagtacc
     4861 tttcacagag tcagctgtat ctaaattgga cctagaggct ggttttattg atgtagaagc
     4921 agaaatacat tcagatggtg aacagagtac agacacagat gaggagagtt ctttatttgc
     4981 cgaggcttct gacaaaatga gtcctggtcc acccatccca ctggacattg ccagcagagg
     5041 cggctatcat gggtcattgg aagaatcatt gggaggtcca atgaaggtgc cgattttgat
     5101 tatccctaaa agtgggattc ctctccctaa tgaaagctct cagtatattg gccctgatat
     5161 gctaccaaat ccatctttaa atactaaggc tgatgattcg cacacagtca aacagaaact
     5221 tgcactaaga ctgtcagaga aaaaaggaca agattctgag ccatcgctca accttctgag
     5281 cccgcacagt aaaggaagca ctgattctgg ttacttttct cgctcagaaa gtgctgagca
     5341 gcaaataagc cctcccaaca caaatgcaaa gtcttatgaa gaaatcatct ttggaaaata
     5401 ctgtcggctt agtccgagaa atgcactcag tgttacaacc acaagtcagg agcgtgccgc
     5461 aatgggtagg aagggcataa tggaaccatt acctcacgtt aacaccaggt tagatgtcaa
     5521 gatgtttgaa gatcctgttt cacagctgat cccaagcaag ggagatgtcg accccagtca
     5581 aacgagcatg ctgaaatcca ctaagttcaa cagtgagtcc agacaacccc agattattcc
     5641 atcatctatc aggaacgaag gaaaacttta tccagcaaac ttccaaggca gcaacccggt
     5701 tctcttagaa gctcctgtag actcttcacc ccttattaga agcaactcag tgccaacttc
     5761 ttcagcaact aatctaacta ttcctccttc tttgagagga agtcactcat ttgatgaaag
     5821 gatgactggt tccgacgatg tattctatcc agggaccgtg ggcatacccc ctcagcgcat
     5881 gctaagaaga caagcggcat ttgagctgcc ttcggtacag gagggccacg tggaagtcga
     5941 gcaccatggc aggatgttga agggtatcag cagttcatcc ctgaaggaaa agaaattgtc
     6001 tcctggggac agggttgggt atgactatga tgtctgtcgg aaaccctata agaagtggga
     6061 ggactctgaa acaccaaagc aaaactacag ggacatttcc tgcttgagtt ctttaaagca
     6121 tggtggagaa tatttcatgg atcccgtggt gccattgcag ggagtaccaa gcatgtttgg
     6181 aactacctgt gaaaacagga aacgccggaa agagaagagc gtaggggatg aagaggacac
     6241 gcccatgatc tgcagcagca ttgtaagcac tcctgtgggc atcatggctt ccgattatga
     6301 ccccaaactg cagatgcagg aaggagtcag gagtggattt gccatggctg gacacgaaaa
     6361 cctttctcat ggtcacacgg aacgctttga cccatgtcgg ccccaactgc agcctggaag
     6421 tccatctctt gtgtcagagg agtcaccttc agccattgat tcagacaaga tgtcagacct
     6481 agggggcagg aaacctcctg gaaatgtgat ttctgtgatt cagcacacca actcactgag
     6541 ccgacccaat tcatttgaaa ggtctgagtc agccgaactt gtggcttgca cacaggataa
     6601 agccccttcc ccttcagaga cttgtgacag tgagatttca gaagccccag tgagtcctga
     6661 gtgggctcca cctggggatg gtgcagaaag tggggggaaa ccctctccat ctcagcaggt
     6721 gcagcagcag tcctatcaca cacagcccag gctagttcgg caacacaaca tccaggttcc
     6781 tgagattcga gtgaccgagg agcctgataa acctgagaag gagaaggaag cccagagcaa
     6841 agagccagag aagcctgtgg aagaatttca gtggccccag agaagtgaga ccctttccca
     6901 gctccccgcg gagaagttgc cacccaaaaa gaagcgtctg cgacttgcag atatggagca
     6961 ctcctcaggg gagtccagct ttgaatccac aggcacaggc ctctcccgca gccccagcca
     7021 agaaagcaac ttgtcccaca gctccagttt ctccatgtct tttgaaagag aagaaaccag
     7081 taagctttct gcacttccta agcaggatga gtttgggaag cattcagagt ttctgactgt
     7141 ccctgctggt tcatactcat tgtctgtccc aggccatcac caccagaaag agatgcgacg
     7201 ctgctcatca gagcagatgc cttgtcctca cccagcggaa gtcccagaag ttcggagcaa
     7261 atcatttgat tatgggaatc tgtcccatgc tcctgtgtcg ggagcagcag cctccacggt
     7321 atcaccgtcc agggagagga agaaatgctt tctggtgcgg caagcttcct tcagtggctc
     7381 cccagaaatc tcccagggcg aggttggcat ggatcagagc gtgaagcaag agcagctgga
     7441 gcacctgcat gctggcctcc ggtccgggtg gcaccatggc ccgcctgctg tgctgcctcc
     7501 tcttcagcaa gaggacccag ggaagcaggt ggcgggtcct tgtcccccgc tgagctcggg
     7561 gccactgcac ctggcccagc cacagatcat gcacatggac agtcaggaat ctttgagaaa
     7621 tcccttgatc caaccaacat cctatatgac aagcaagcac ttacctgaac agccacactt
     7681 atttccacat caagagacaa ttccattttc tccaatccag aatgccttgt ttcagtttca
     7741 gtatcctaca gtttgtatgg ttcatttacc agctcagcag cctccctggt ggcaggcaca
     7801 tttcccacat ccctttgctc agcaccctca gaagagctat ggcaagccct cttttcagac
     7861 agaaatccat tcgagctatc ccttagagca tgtggcagag cacactggaa agaaacctgc
     7921 tgagtatgca cacacgaaag agcagaccta cccatgttat tcaggagcat cagggctaca
     7981 cccaaagaac cttcttccaa agtttccatc agaccagagc agtaagtcaa ctgaaacgcc
     8041 ctctgagcag gttcttcaag aagattttgc ctcggcaaat gctgggtctt tgcagtccct
     8101 cccaggaaca gtggttcctg ttcggatcca gacgcacgta ccatcctatg gaagtgtcat
     8161 gtacacaagc atttctcaga tacttgggca gaatagccct gccattgtca tatgcaaagt
     8221 cgatgagaat atgacccaaa ggacactggt caccaacgca gccatgcaag ggataggatt
     8281 caacattgcc caggtgctgg ggcagcatgc gggcttggag aagtacccca tttggaaagc
     8341 acctcagact ttgcccctcg gcttagaatc ctccatcccc ttgtgtttac cttccacctc
     8401 tgacagcgtg gccaccctgg gaggtagcaa gcgaatgctt tctccagcca gtagcttgga
     8461 gctcttcatg gaaaccaagc agcagaaaag ggtcaaagaa gaaaagatgt acggacagat
     8521 tgtggaggag cttagtgctg tggagctgac caactcagac atcaaaaagg acctctcccg
     8581 cccccagaaa ccccagctgg ttcgacaagg atgtgcttct gagccaaaag atggcttgca
     8641 gtcagggtca tcttccttct cctcgctgtc gccctcctca tctcaagact atccttctgt
     8701 tagcccgtct tccagggagc cattcctgcc cagcaaggag atgctttccg gttcccgggc
     8761 accacttccg gggcagaagt ccagtgggcc ttctgaaagc aaagaatctt cagatgaatt
     8821 agatatcgat gagacggcat cggacatgag catgagccca cagagttctt cattaccagc
     8881 aggagatggt cagctggaag aggaagggaa gggccacaag cggcctgttg gcatgctggt
     8941 ccgcatggcc tctgccccca gcgggaacgt ggcagactca actcttcttc tcacggacat
     9001 ggcagatttc cagcagattc ttcagttccc cagtctgcgg acaacaacta ctgtgagttg
     9061 gtgcttcttg aattatacaa aacccaatta tgtgcaacag gccaccttca aatcctcggt
     9121 ttatgcttca tggtgcatta gttcctgtaa tccaaaccca tcaggattga acaccaagac
     9181 cacgctggct cttctgaggt ccaagcaaaa aatcactgca gaaatttata ctctggctgc
     9241 tatgcatagg cctggaaccg gcaagcttac atcatcaagt gcttggaagc agtttactca
     9301 gatgaaacct gatgcgtcct ttttatttgg cagcaaacta gaaaggaaac tagtgggaaa
     9361 tatcttaaag gaaagaggga aaggagatat tcatggagat aaagatattg gatccaaaca
     9421 aactgagcca atccgaatta aaatatttga aggagggtac aaatcgaatg aagattatgt
     9481 atatgtcaga ggacgtggcc ggggaaagta catttgtgaa gaatgtggga ttcgctgtaa
     9541 gaagccaagc atgctcaaaa aacacatccg tacccatact gatgttcggc cttatgtatg
     9601 caagttatgt aactttgcct tcaaaacgaa aggaaaccta acgaagcata tgaaatctaa
     9661 agcacacatg aaaaaatgcc tggaattggg agtctcaatg acatcggtgg atgatacaga
     9721 aactgaggaa gcagaaaatt tggaagattt gcacaaagca gcagagaagc atagcatgtc
     9781 cagcatttca actgatcatc agttctccga tgctgaggaa tcagatggtg aggatggaga
     9841 tgataatgat gatgatgatg aagatgaaga tgactttgac gaccagggag atttaacacc
     9901 aaaaacaaga tcaagaagca ccagtcctca gcctcctaga ttctcctcct tgcctgtgaa
     9961 tgttggcgcc gtaccccacg gggttccttc agatagttcc ctgggacatt cttcgttgat
    10021 cagctatttg gttactttgc caagtattcg agttactcag cttatgacac ccagtgattc
    10081 atgtgaagat acccagatga cagaatacca gaggctattc cagagcaaaa gtacggactc
    10141 agaaccagac aaagacagat tggacatacc tagttgtatg gatgaggagt gcatgctacc
    10201 ttcagagcca agctcctctc ccagggactt ctcaccctca agccaccatt cctctccagg
    10261 atatgattct tcaccctgtc gagataattc accaaagagg tatctgatac ccaaaggaga
    10321 tttatctccc aggagacatt tatcacctag gagagatctg tcacccatga gacatctttc
    10381 accaagaaag gaagctgcat tgagaagaga gatgtcccaa agagatgttt caccaagaag
    10441 gcatttgtct ccaaggaggc cagtgtctcc tgggaaagat atcacagcaa gaagagacct
    10501 ctctcctaga agagagagaa gatacatgac cacaataaga gcgccatctc ccagaagggc
    10561 tttataccat aacccaccat tgtccatggg acagtatttg caagcagagc caattgtatt
    10621 ggggcctcct aatttaagaa gaggattacc tcaggttcct tacttcagtc tctatggaga
    10681 ccaagaaggt gcttatgaac atccaggctc cagccttttc cctgagggtc ctaatgacta
    10741 tgtcttcagt catcttccac tccactctca gcaacaagtg cgagccccta tccccatggt
    10801 gcccgttggt gggatccaga tggttcactc catgccgcca gccctttcca gtttacatcc
    10861 ttcacccaca ttgcccctgc caatggaggg ctttgaggag aagaaaggcg cgtcagggga
    10921 gtccttctcc aaggacccct atgtgctttc taagcagcat gagaagcgag gtcctcacgc
    10981 tttgcagtca tctggtccac ctagcactcc ctcctctcct cggctgttga tgaaacagag
    11041 cacttcggaa gacagcctaa acgcaacaga gcgggaacag gaggaaaata tacagacttg
    11101 tacaaaagcc attgcctctc tccggattgc cacggaagag gcagctctgc tcgggccaga
    11161 tcagccagcg cgggtgcagg agccccacca gaaccccctg ggaagtgcac atgttagcat
    11221 tagacacttt agtagacctg agccaggtca gccctgtacc tcagccaccc accctgactt
    11281 gcatgatggt gaaaaggaca attttggtac atcacagact ccattagctc actccacgtt
    11341 ttacagcaag agttgtgtgg atgacaagca gttggacttt cacagcagca aggaattatc
    11401 ttcaagcaca gaggaaagca aagatccttc atcagaaaag agtcagctac attagtaatg
    11461 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
    11521 cgagagatgc tgctcagagt tgctccgacg cgttaagtcg acaatcaacc tctggattac
    11581 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
    11641 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
    11701 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
    11761 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    11821 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    11881 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    11941 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
    12001 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    12061 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
    12121 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
    12181 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
    12241 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
    12301 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    12361 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    12421 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
    12481 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
    12541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    12661 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    12721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    12781 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    12841 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    12901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    12961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    13021 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    13081 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    13141 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    13201 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    13261 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    13321 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    13381 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    13441 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    13501 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    13561 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    13621 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    13681 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    13741 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    13801 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    13861 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    13921 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    13981 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    14041 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    14101 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    14161 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    14221 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    14281 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    14341 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    14401 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    14461 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    14521 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    14581 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    14641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    14701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    14761 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    14821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    14881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    14941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    15001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    15061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    15121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    15181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    15241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    15301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    15361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    15421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    15481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    15541 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    15601 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    15661 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
