LOCUS       sequence-397922-       11902 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0680
FEATURES             Location/Qualifiers
     source          1..11902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(4700..4711)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    7759..8347
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7812..7832)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8230..8241)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8419..8652
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8730..8851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8767..8786)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8821..8840
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8891..9026
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9046..9065)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9047..9065)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9075..9092)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9075..9091)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9084..9106)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9233..9688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9320..9339)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9530..9551
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9714..9818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9819..9887,9888..10679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10037..10056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10850..11438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11339..11358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11592..11609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11726..11747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11762..11767,11768..11785,11786..11792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11800..11816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11805..11827
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11824..11840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11824..11840
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11859..11879
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11861..11879
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgccc cgccgccgcc ccccggcttc
     4141 cggtgctgcg cagtttccgg agcggatcgc aacccggagt ccggatccga tcccgctctg
     4201 cacattccaa aggcaggtgt cagagatggc tgaagatggg agcgaagaga tcatgttcat
     4261 ctggtgtgaa gactgcagcc agtaccacga ctccgaatgt cccgagctgg gcccagtggt
     4321 catggtcaaa gactcctttg tgttaagcag ggcaaggtca tcccttcctc ccaacttgga
     4381 gatcagacga ctggaagatg gagccgaggg ggtgttcgcc atcactcagc tcgtcaagcg
     4441 gacacagttc ggtccctttg agtccaggag ggtcgccaaa tgggaaaagg agtctgcatt
     4501 tcccctgaag gtgttccaga aggacgggca ccccgtgtgc ttcgacacct ccaacgagga
     4561 tgactgcaac tggatgatgc tggtgcggcc agcggcggag gccgagcacc agaacctgac
     4621 ggcctaccag cacggcagcg acgtgtactt caccacctcc agagacatcc ccccgggtac
     4681 cgagctgcgc gtgtggtatg cggccttcta tgccaagaag atggacaagc ccatgctgaa
     4741 gcaggccggc tctggcgtcc acgctgcagg caccccagaa aacagcgccc ccgtggagtc
     4801 ggagcccagc cagtgggcgt gtaaagtgtg ttctgccacc ttcctggagc tgcagctcct
     4861 caatgaacat ctgttgggcc acttagaaca agccaaaagc cttcctccag gcagccaaag
     4921 cgaggcagca gctcccgaga aggagcagga cacaccccgg ggggaacccc ctgcagtgcc
     4981 cgagagcgag aatgttgcca ccaaagaaca gaagaaaaag cctcgaaggg ggagaaaacc
     5041 caaagtgtcc aaagctgagc agcctctagt catcgtggaa gacaaggaac ccacagagca
     5101 agtggcagag atcattaccg aggtccctcc ggatgagcct gtgagtgcaa cgccagatga
     5161 gcggatcatg gagctggttc tggggaagct ggccaccacc accactgaca ccagctcggt
     5221 tccaaagttc acccatcatc agaataacac catcacgctc aagaggagct taattctctc
     5281 aagcagacac ggcatccggc gcaagctcat caaacagctc ggggagcaca agcgggttta
     5341 ccagtgcaat atctgcagca agatcttcca gaacagcagc aacctgagca ggcacgtgcg
     5401 ctcgcatggt gacaagctgt ttaagtgcga agagtgtgca aaattgttca gccgcaaaga
     5461 gagcctaaag cagcacgttt cctacaagca cagcaggaac gaggtggacg gcgagtacag
     5521 gtaccgctgc ggcacttgtg agaagacctt ccgcatcgag agcgcgctgg agttccacaa
     5581 ctgcaggaca gatgacaaga cgttccaatg tgagatgtgt ttcagattct tctccaccaa
     5641 cagcaacctc tccaagcaca agaagaagca cggcgacaag aagtttgcct gtgaggtctg
     5701 cagcaagatg ttctaccgca aggacgtcat gctggaccac cagcgccggc acctggaagg
     5761 agtgcggcga gtgaagcgag aggacctgga ggccggtggg gagaacctgg tccgttacaa
     5821 gaaggagcct tccgggtgcc cggtgtgtgg caaggtgttc tcctgccgga gcaatatgaa
     5881 caagcacctg ctcacccacg gcgacaagaa gtacacctgc gagatctgcg ggcgcaagtt
     5941 cttccgcgtg gatgtgctca gggaccacat ccatgtccac ttcaaggaca tcgcgttgat
     6001 ggatgaccac cagagggaag agtttatcgg caagatcggg atctcctcgg aagaaaacga
     6061 tgacaattct gacgagagcg cagactcgga gcctcacaag tacagctgca agcggtgcca
     6121 gctcaccttc ggccggggga aggagtacct gaagcacatc atggaggtgc acaaggagaa
     6181 gggctatggc tgcagcatct gcaaccggcg ctttgcactg aaggccacct accacgccca
     6241 catggtcatc caccgtgaaa acctgccgga ccccaacgtg cagaagtaca tccacccctg
     6301 cgagatctgc gggcggatct tcaacagcat cgggaacctg gagcgccaca agctcatcca
     6361 cacaggtgtg aagagccacg cctgcgagca gtgtgggaag tcctttgcca ggaaggacat
     6421 gctgaaggag cacatgcgtg tgcacgacaa tgtccgcgag tacctgtgtg ccgagtgtgg
     6481 gaaaggcatg aagaccaagc acgcgctgcg ccaccacatg aagctgcaca agggcatcaa
     6541 ggagtacgag tgcaaggagt gccaccgcag gttcgcgcag aaggtcaaca tgctcaagca
     6601 ctgcaagcgg cacacgggga ttaaagattt catgtgtgaa ttgtgtggga agacattcag
     6661 cgagaggaac accatggaga cccacaagct catccacaca gtgggcaagc agtggacgtg
     6721 ctccgtgtgc gacaagaagt acgtgaccga gtacatgctg cagaagcacg ttcagctcac
     6781 acacgacaag gtggaggcgc agagctgcca gctgtgcggg accaaggtgt ccaccagggc
     6841 ctccatgagc cgacacatgc ggcgcaagca ccccgaggtg ctcgcggtga ggatcgatga
     6901 cctggaccac ctcccggaga ccaccaccat cgacgcctcc tccattggca tcgtccagcc
     6961 tgagctgact ctggagcagg aggatttggc cgaagggaag cacgggaaag ctgccaagcg
     7021 aagtcacaag agaaagcaga agccagaaga ggaggcgggt gctccggtgc ccgaggacgc
     7081 caccttcagc gaatactcag agaaagagac ggagttcaca ggcagtgtag gcgacgagac
     7141 caattccgca gtacagagca ttcagcaggt agtggtgacc ctgggtgacc caaatgtgac
     7201 cacaccatcg agctcagtcg gcttaaccaa catcaccgtg acccccatca ccactgcggc
     7261 cgcgactcag tttaccaatc tccagccggt ggccgtgggg caccttacca cccctgaacg
     7321 ccagttacag ctggacaact caatcctgac cgtgaccttt gataccgtca gcggctctgc
     7381 catgttgcac aaccgccaaa atgacgtcca gatccacccc cagccggaag cctcgaaccc
     7441 acagtctgtg gcccatttca tcaacctgac gaccctggtc aactccatca cgcccctggg
     7501 gagccagctt agtgaccagc acccgctcac gtggcgggca gtgccccaga ctgacgtctt
     7561 gccaccctcg cagccgcagg cacccccaca gcaggcggcc cagccccagg tgcaggcgga
     7621 gcagcagcag cagcagatgt acagctacta gtaatgaact agtccgtaac ttgaaagtat
     7681 ttcgatttct tggctttata tatcttgtgg aaaggacgaa gaaccatgaa gcggatttac
     7741 tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7801 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7861 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7921 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7981 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8041 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8101 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8161 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8221 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8281 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8341 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8401 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     8461 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8521 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8581 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8641 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     8701 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     8761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     8881 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8941 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9001 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     9061 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9121 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9181 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     9241 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9301 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9361 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9421 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     9481 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     9541 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     9601 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     9661 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     9721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    10081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    10321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    10381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    10441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    10501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    10561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    10621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    10681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    10741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    11041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    11281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    11401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    11461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    11521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    11581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    11641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    11701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    11761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    11821 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    11881 aacaaaagct ggagctgcaa gc
//
