LOCUS       sequence-399432-        8531 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Inducible expression of AjTn6022 proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 8531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT385: pHelper(AjTn6022)_QF-separated
FEATURES             Location/Qualifiers
     source          1..8531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             4765..4776
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      6435..6482
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6653..7444)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(7445..7536)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7584..8322)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg aaaagaatca gtgatgaata ttccccagtt aggaaatccc
      121 aaactgtata tgggagtatt tcagtaaatt atgcttttag aggggaaaag acgatttggt
      181 gtgaatctac tcttgagcgt gattttttga ttaggcaaga attcaatagc aatgtcatag
      241 atatcgtaag ccagcctatt accatcccat atataacaga gttgggtaat gaatccactt
      301 atactcctga tttcctcgta cagttttcca gtgcaaattg tggtgatttt gaggaaattc
      361 cctgtccact gttaatagaa ataaaaccta gaaagaaact tatagaagat tgggataaat
      421 taaagccaaa atttaaggcg gctcatactt ttgctaaaga aaaaggttgg aaattcaaga
      481 ttataaatga atcaaggtta tatgatcagt attgggaaaa tattaatttt ttgaaaagat
      541 ttagacgttc tcatgtggat gtctatgatg aacaaacttt aattgagact ttagatagtc
      601 ttggtcaatc gactattaat caattaccgg cacatatttt taaggataaa actaatattc
      661 ttcgaggtat tcatcaagta tgggcattgg tcgcaaaacg aaaaatcatg tgtgatttat
      721 attgcccctt aacagcagag acggtgattt ggacaaatca agatgatgtg cttacaagga
      781 gatttttaaa tggttcagac tgatactgta agtattgacc gaagtcggat catactcaaa
      841 ccaaatgtaa ttgttaagta tgaagggcaa ccgtataaga ttgctaatgt tttgaatgcc
      901 aacgatatcg tgatttctag tttagattct gtgcgttgtt tacaagtaag tgcagattct
      961 ctacaagtat ttgaagctga aaataaccaa actaaagata taaataaagg tgattgggat
     1021 attacgcatg aagcgtggaa aatagcttta catcgttatg aaatcatcaa gccgttgatt
     1081 aagtattcaa caaccgaatt agtcgagcaa cgagcaagtg aatatggcat taaccgttcc
     1141 accttatgga aatggttaaa agattttaga gaaaataatt ctatcttagt gttagttcct
     1201 aaaaagagag gttggacgac caagaagtct aggttgtctc ctcaaattat gaatattatt
     1261 aaccaaggta ttcaagagga atatctcaat gccagaaagc ctagtattgc aaaaactata
     1321 gattttatta aagcagaatg ttctcgcctt aaactagaag caccccatga caattcaatt
     1381 cgtagaaaaa tagaagcatt aaacgattat atggtgatta aggctcgtca tggttctaaa
     1441 gcagcacaag ataagtatgc atctgctgct ggtgagtttc ctaatgctga ttatcccttg
     1501 gcatatgtac aggtagacca cactccacta gatattgaaa ttgttgatga tgaatttaga
     1561 gaggcgattg gacgaccatt tctaacatta gctattgata taaatactcg aatggtggtc
     1621 ggttattact tgtccttaga agcccccagt gcaacatctg ttgccatgtg tattgcttcc
     1681 agtattttgt caaaaaaacg gaagctaatt gaattggata tagatgctga ttggcaagtt
     1741 gaaggtctta tggattctat acatacagat aatggtcctg actttagaac taaccatatc
     1801 agtcgagctt gcttaaaata tgggattcat tgggagtttc gtccgattgg tggggcaagg
     1861 tttggtggac atatcgaacg tttgattggt gttgttaaca ttgaaatgca catcttggat
     1921 ggtacaacct tctcgaatat ccaagaacgt ggaacctata attcagcagg tcatgcctgt
     1981 atgacactaa aagaactaga atattatatt gtttattgga tcactaaagt ttatcaccaa
     2041 aagaaacact ccaaactcgg tatgtcacct ttacagaagt ggcaagatgg tgtttgggga
     2101 acaaaaacta gtgttggaac aggtttaaaa gagagagttt cggatgaaga tacattattt
     2161 attgatttcc ttcccgaatt tgaatcaaca attcaacgtg taggtgtaca gaaagacaat
     2221 ctattttatt ttgctgattg tttacgacaa tgggttaatt gtgttgatcc cgaagatgta
     2281 aataaaaaga gaaaaaagaa atttttgttt aaacgtgatc caagggatat ttctcttatt
     2341 tggttttatg aacctctgtc caatacatat tttaaagtgc ctactgctaa gcgagaaata
     2401 ccaccaatta gcttatttga atacaaacaa gtgcagaaat atcttcgtgg cgagcaacat
     2461 gataatcaaa atcaggatga gatttatcag gcaattcttc atttacgaga gcaattgaaa
     2521 caatcccgta gtctgactcg gaagcaacgg agagtaaatc aacgtaaaaa ggaaaatgca
     2581 aaagcaacac atcatttatc tgtaaaaaat gaacctgaaa aaccatctat tgtcgaaaac
     2641 agtgttcagc cgactaatga tttatggagt attccattga cggcatttga agatttaagg
     2701 ggataataat gcaaaaatat gaacatcttg cagatgatgt acttgaagtc atgtctctaa
     2761 gtgatagtga tcgtatcaat attttatttt ctgatcgttg gattggttat aaaaaagcgg
     2821 tcactatttt ggatacttta actgatctgt tgaatcgccc taggaagctt cgcccagaat
     2881 gtttattgat tgtgggtgat tctaatatgg gtaaaacgac cattatccac gaattcacaa
     2941 agcagcatta caccaaaaca gtgaatgaca tagatatgga gctgctgtct gtaaaaaaac
     3001 ctgttttaac catattagcc cctgctaaag cgaatgtaaa agaattctat atcaatatat
     3061 tgaatcattt ctttgtccca tttcgtccta ctgatcctga agcaaagtta cggaatcaag
     3121 ctgtccatct aatgcggaag tatgaaacaa aaatgttgat tattgatgaa atccataatt
     3181 gtttaacagg ttctaccaaa cttctttccg aagtgatgaa taccttaaag aatctaagta
     3241 atgatctaag tctgaatatc gttggcgtcg gtactcgtga agcagttacg attctacata
     3301 ccgatgctca atatgcaagc cgttttgatg tagcaatgtt gcctaaatgg gaactgaatc
     3361 aggattttct aagactatta ttaagttatg tacgattatt gcctttaaag aaacgttctg
     3421 atctagcatc tcaaagactt gccaccctta tatttgaaat ttcaggtggg aattttggtg
     3481 acttaaacag attgttgatt gagtgtgcta aagaggcaat cgtaaaaggt aatgaagaaa
     3541 ttaccgaaga aattgttaat aaatttaaat ggcttaaacc aaccgagggt atgaggaata
     3601 ttagaaatat aaatcttaac atggtttagg tattctactt ttcataggaa aatagccatg
     3661 caccaagtag aacaacgttg gtatattcgt acccctattc aagagggtga agtattttcc
     3721 tcttggctca tacgttctgc tttggattta ggctgctcac cattaacttt agtgaatctt
     3781 ttgtgggaaa aatggagagg attaacgata gatattgatc gtgggttaaa tgaagaaaaa
     3841 ttagacatat tgttaaatca ttgttttgtg gaacagcatg aagttcagaa aactatgtta
     3901 agcagttatt tccctgcttt gattccaaaa aattataaca aaaatcagcg tattccttgg
     3961 atattggctc taggtattcg aaatcgttca aatgtgtcag gtcgtcaaat ttgtacgcaa
     4021 tgtctaagtt ctacccatac accaccatat ttgcgtatca agtggcgtat ggggtggcat
     4081 agtggctgtg atattcataa aaccatatta atagatcatt gtcctcaatg cagaatcaca
     4141 ttacaaccta taaaaattga tatagagcat ggatcattag caatttgtac ttcatgctat
     4201 gctgatttaa gtacactcat tcaacgtcca gtagaaacgg atgcattaag atttcagcat
     4261 caagctgatc aggtcttaga gtcacaattt gggcgatata accaaaagga tatttcatca
     4321 atagagtggt ttgagattgc tcgtatatgg ttaagtttta ttcgatcccc tttaaatatg
     4381 aagagtccta accttttgca aatgtttgaa agtcttaata ttgatttaaa tatagattat
     4441 cctattacac cagtagcctt tgaatttctg tctgttcagg agcgtgaaaa attattatca
     4501 attttaaata aaatcatggc attaccttgt gatttatttg ttgatcgctc tttagaatat
     4561 catgtaagtc attcttattt ttgggataaa agaaaaaaac tgcctacgca gcttcaacta
     4621 atgaaagata aaatgttgaa gccaattaag attattttgc cgaaagataa cgccatagcc
     4681 atgagtaaac ccaaatctaa aaaaagtgta caacggaagt ggttaaggct attgagaaag
     4741 ctacagaaag gggggaacag gtgaaaagag gagaaaggat ctatggtgaa agcgattgaa
     4801 aaagcgaccg aacgtggaga acaagttact ttagctcaat tttcccataa agaaggctgt
     4861 agtggttgtg acagggctga tatagataaa ttcaaaaaaa gatataaggg cgataagtat
     4921 tgttcaactt gttatgcccg aatttttaaa aaaagagctt gtccaaaatg tggtgaatat
     4981 gccagattgc caaaaaatga tgatcaagca atttgtaatg aatgcatcaa aaaacagcct
     5041 tgtattcgct gtaatcaaac taagaagccg atagggacac tcaccgaata tggtgtggtt
     5101 tgtaatagct gttctgttta ttttcgccca attgagcgtt gtgagaggtg tgatacacca
     5161 tcacaaaaat tgacacatat ttcacgattt ggtgatgatt taagggtatg tcccaaatgc
     5221 tctactagag attatgaaac atgtccctct tgtcataaat atcgcttatt agagcaagat
     5281 gatacaggag ctaagatttg taaaaaatgt aggaataacg ccaaaaagaa atgcaagcaa
     5341 tgtgatgtct tgataccagc aggctgtcct gatctatgta acaactgtta ctggcataag
     5401 aatctatggg agaaagctag acgaaatatt aaagcattcc aatcccaaca cttacaggct
     5461 caatatgagc aatatttaat ttggttagaa gatgaggttg gtgcaaataa agcagcactg
     5521 tatatcaata aacataccca tttttttatc aaaactgaag aattatggct tgatgcaatt
     5581 cctacagcag aacagttatt ggctgtttta agaaccagtg gacttagaaa atttgagttg
     5641 gttgcatctt ggctagatga agcccatcac attaaggttg cattagagga taaagacttt
     5701 tgctctcaac aggatcagat agaaaaactg atttcaagcc ttcctcaccc ctctactgct
     5761 tatgatgtgg ttatatctta taaagatgag cttgatataa aaatgaaaga tggaaagacc
     5821 agtatccggt ccataaaact ggcaataaag cctgcggttg ctcttatgca ctatgtttgt
     5881 gcatcgggag ctacattacc taatttaaac catataaaag cctatcttat cgacttttct
     5941 ggtcaggctg ctgcactaac agggttcatt aattttttga ataaaaattt tgatacatct
     6001 atagactatt tggcttttaa aaaatcaaaa aatttcaatg aaaaacgaaa aaataaagta
     6061 gaaaaagaaa ttgtccaatg ggtggataag cctttggaga ataaggaaga tgtattaaat
     6121 tgggtgaaga atgggctaag atattttcat aatgtatctt atgtggagtc tctaaaagtc
     6181 aaatttgaga tgattacaga agctgatgat ggatatgaaa ttttattgca gaatcattct
     6241 tattggttgc caaaaaacac aggagattta aaaagatagt ttttttgtga ttatactgtg
     6301 ctatttcaag ctaatttatt gtacaagttg gtttgtttat ctaattcttc taaaagaact
     6361 gtccataaca atatggggca ggctgctgcc accgctgagc aataacctag gctgctgcca
     6421 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     6481 tgctgaaacc tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac
     6541 cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg
     6601 ggtcatcgtg gccggatctt gcggcccctc ggcttgaacg aattgttaga cattatttgc
     6661 cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc
     6721 gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg
     6781 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga
     6841 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat
     6901 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata
     6961 gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc
     7021 tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga
     7081 agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg
     7141 gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa
     7201 tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg
     7261 ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca
     7321 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc
     7381 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc
     7441 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7501 gatacatatt tgaatgtatt tagaaaaata aacaaatagc tagctcactc ggtcgctacg
     7561 ctccgggcgt gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg
     7621 tggataagca ggggactaac atgtgaggca aaacagcagg gccgcgccgg tggcgttttt
     7681 ccataggctc cgccctcctg ccagagttca cataaacaga cgcttttccg gtgcatctgt
     7741 gggagccgtg aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa
     7801 gatccccacc gtttccggcg ggtcgctccc tcttgcgctc tcctgttccg accctgccgt
     7861 ttaccggata cctgttccgc ctttctccct tacgggaagt gtggcgcttt ctcatagctc
     7921 acacactggt atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa
     7981 ctccccgttc agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg
     8041 gaaaagcacg gtaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat
     8101 ttgtttagct aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa
     8161 ggacagattt ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa
     8221 ctgncttaac cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag
     8281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgctc
     8341 tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat
     8401 aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     8461 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa
     8521 ttgcgttgcg c
//
