LOCUS       sequence-399659-       12300 bp    DNA     circular SYN 14-APR-2025
DEFINITION  endolysosome localized pH sensor using sensorsuperecliptic pHluorin 
            (SEP) and mRuby3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Liu B, Carlson RJ, Pires IS, Gentili M, Feng E, Hellier Q, Schwartz 
            MA, Blainey PC, Irvine DJ, Hacohen N
  TITLE     Human STING is a proton channel.
  JOURNAL   Science. 2023 Aug 4;381(6657):508-514. doi: 10.1126/science.adf8974.
            Epub 2023 Aug 3.
  PUBMED    37535724
REFERENCE   2  (bases 1 to 12300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adf8974"; journalName: "Science"; date: "2023-08-4-
            4"; volume: "381"; issue: "6657"; pages: "508-514"
COMMENT     Sequence Label: LAMP1-SEP-mRuby3
FEATURES             Location/Qualifiers
     source          1..12300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3727..3736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3731..3740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3820..4527
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     CDS             4540..5253
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4570..4598)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5193..5213
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     LTR             6704..6884
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8412..8431)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8645..8664)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8764..8786
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8824..8842)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8910..9014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9015..9083,9084..9875)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9233..9252)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10046..10634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10535..10554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10788..10805
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10875..10895)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10880..11209
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11060..11195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcggcc gcgggctagc
     3721 ggatccgcca ccatggcggc ccccggcagc gcccggcgac ccctgctgct gctactgctg
     3781 ttgctgctgc tcggcctcat gcattgtgcg tcagcagcag tgtctaaggg cgaagagctg
     3841 atcaaggaaa atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg ccaccaattc
     3901 aaatgcacag gtgaaggaga aggcagaccg tacgagggag tgcaaaccat gaggatcaaa
     3961 gtcatcgagg gaggacccct gccatttgcc tttgacattc ttgccacgtc gttcatgtat
     4021 ggcagccgta cctttatcaa gtacccggcc gacatccctg atttctttaa acagtccttt
     4081 cctgagggtt ttacttggga aagagttacg agatacgaag atggtggagt cgtcaccgtc
     4141 acgcaggaca ccagccttga ggatggcgag ctcgtctaca acgtcaaggt cagaggggta
     4201 aactttccct ccaatggtcc cgtgatgcag aagaagacca agggttggga gcctaataca
     4261 gagatgatgt atccagcaga tggtggtctg agaggataca ctgacatcgc actgaaagtt
     4321 gatggtggtg gccatctgca ctgcaacttc gtgacaactt acaggtcaaa aaagaccgtc
     4381 gggaacatca agatgcccgg tgtccatgcc gttgatcacc gcctggaaag gatcgaggag
     4441 agtgacaatg aaacctacgt agtgcaaaga gaagtggcag ttgccaaata cagcaacctt
     4501 ggtggtggca tggacgagct gtacaaggga tccgccacca tgagtaaagg agaagaactt
     4561 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     4621 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     4681 tgcactactg gaaaactacc tgttccttgg ccaacacttg tcactacttt aacttatggt
     4741 gttcaatgct tttcaagata cccagatcat atgaaacggc atgacttttt caagagtgcc
     4801 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     4861 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     4921 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataacgat
     4981 caccaggtgt acatcatggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
     5041 agacacaaca ttgaagatgg aggcgttcaa ctagcagacc attatcaaca aaatactcca
     5101 attggcgatg ggcccgtcct tttaccagac aaccattacc tgtttacaac ttctactctt
     5161 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     5221 gggattacac atggcatgga tgaactatac aaaggctcag gctcagcaat gtttatggtg
     5281 aaaaatggca acgggaccgc gtgcataatg gccaacttct ctgctgcctt ctcagtgaac
     5341 tacgacacca agagtggccc taagaacatg acctttgacc tgccatcaga tgccacagtg
     5401 gtgctcaacc gcagctcctg tggaaaagag aacacttctg accccagtct cgtgattgct
     5461 tttggaagag gacatacact cactctcaat ttcacgagaa atgcaacacg ttacagcgtc
     5521 cagctcatga gttttgttta taacttgtca gacacacacc ttttccccaa tgcgagctcc
     5581 aaagaaatca agactgtgga atctataact gacatcaggg cagatataga taaaaaatac
     5641 agatgtgtta gtggcaccca ggtccacatg aacaacgtga ccgtaacgct ccatgatgcc
     5701 accatccagg cgtacctttc caacagcagc ttcagcaggg gagagacacg ctgtgaacaa
     5761 gacaggcctt ccccaaccac agcgccccct gcgccaccca gcccctcgcc ctcacccgtg
     5821 cccaagagcc cctctgtgga caagtacaac gtgagcggca ccaacgggac ctgcctgctg
     5881 gccagcatgg ggctgcagct gaacctcacc tatgagagga aggacaacac gacggtgaca
     5941 aggcttctca acatcaaccc caacaagacc tcggccagcg ggagctgcgg cgcccacctg
     6001 gtgactctgg agctgcacag cgagggcacc accgtcctgc tcttccagtt cgggatgaat
     6061 gcaagttcta gccggttttt cctacaagga atccagttga atacaattct tcctgacgcc
     6121 agagaccccg cctttaaagc tgccaacggc tccctgcgag cgctgcaggc cacagtcggc
     6181 aattcctaca agtgcaacgc ggaggagcac gtccgtgtca cgaaggcgtt ttcagtcaat
     6241 atattcaaag tgtgggtcca ggctttcaag gtggaaggtg gccagtttgg ctctgtggag
     6301 gagtgtctgc tggacgagaa cagcatgctg atccccatcg ctgtgggtgg tgccctggcg
     6361 gggctggtcc tcatcgtcct catcgcctac ctcgtcggca ggaagaggag tcacgcaggc
     6421 taccagacta tctaactcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     6481 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     6541 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6601 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaaaatc
     6661 gtcgagagat gctgcatata agcagctgct ttttgcttgt actgggtctc tctggttaga
     6721 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6781 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6841 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6901 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
     6961 acattataat agatttagca ggaattgaac taggagtgga gcacacaggc aaagctgcag
     7021 aagtacttgg aagaagccac cagagatact cacgattctg cacatacctg gctaatccca
     7081 gatcctaagg attacattaa gtttactaac atttatataa tgatttatag tttaaagtat
     7141 aaacttatct aatttactat tctgacagat attaattaat cctcaaatat cataagagat
     7201 gattactatt atccccattt aacacaagag gaaactgaga gggaaagatg ttgaagtaat
     7261 tttcccacaa ttacagcatc cgttagttac gactctatga tcttctgaca caaattccat
     7321 ttactcctca ccctatgact cagtcgaata tatcaaagtt atggacatta tgctaagtaa
     7381 caaattaccc ttttatatag taaatactga gtagattgag agaagaaatt gtttgcaaac
     7441 ctgaatagct tcaagaagaa gagaagtgag gataagaata acagttgtca tttaacaagt
     7501 tttaacaagt aacttggtta gaaagggatt caaatgcata aagcaaggga taaatttttc
     7561 tggcaacaag actatacaat ataaccttaa atatgacttc aaataattgt tggaacttga
     7621 taaaactaat taaatattat tgaagattat caatattata aatgtaattt acttttaaaa
     7681 agggaacata gaaatgtgta tcattagagt agaaaacaat ccttattatc acaatttgtc
     7741 aaaacaagtt tgttattaac acaagtagaa tactgcattc aattaagttg actgcagatt
     7801 ttgtgttttg ttaaaattag aaagagataa caacaatttg aattattgaa agtaacatgt
     7861 aaatagttct acatacgttc ttttgacatc ttgttcaatc attgatcgaa gttctttatc
     7921 ttggaagaat ttgttccaaa gactctgaaa taaggaaaac aatctattat atagtctcac
     7981 acctttgttt tacttttagt gatttcaatt taataatgta aatggttaaa atttattctt
     8041 ctctgagatc atttcacatt gcagatagaa aacctgagac tggggtaatt tttattaaaa
     8101 tctaatttaa tctcagaaac acatctttat tctaacatca atttttccag tttgatatta
     8161 tcatataaag tcagccttcc tcatctgcag gttccacaac aaaaatccaa ccaactgtgg
     8221 atcaaaaata ttgggaaaaa attaaaaata gcaatacaac aataaaaaaa tacaaatcag
     8281 aaaaacagca cagtataaca actttattta gcatttacaa tctattaggt attataagta
     8341 atctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     8401 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     8461 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     8521 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     8581 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     8641 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     8701 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8761 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     8821 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     8881 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8941 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9001 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9061 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9121 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9181 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9241 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9301 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     9361 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     9421 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9481 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9541 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9601 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9661 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9721 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9781 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9841 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9901 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9961 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10021 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10081 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10141 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10201 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10261 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10321 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10381 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10441 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10501 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10561 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10621 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10681 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10741 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10801 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10861 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    10921 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    10981 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    11041 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    11101 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    11161 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
    11221 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    11281 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    11341 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    11401 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
    11461 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    11521 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    11581 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    11641 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    11701 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    11761 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    11821 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    11881 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    11941 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    12001 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    12061 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    12121 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    12181 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    12241 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
//
