LOCUS       sequence-399703-       17893 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express Arabidopsis thaliana LYK5 gene under UBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17893)
  AUTHORS   Trinh J, Li T, Franco JY, Toruno TY, Stevens DM, Thapa SP, Wong J, 
            Pineda R, Avila de Dios E, Kahn TL, Seymour DK, Ramadugu C, Coaker 
            GL
  TITLE     Variation in microbial feature perception in the Rutaceae family 
            with immune receptor conservation in citrus.
  JOURNAL   Plant Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263.
  PUBMED    37144828
REFERENCE   2  (bases 1 to 17893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263."
COMMENT     Sequence Label: pUBQ10:LYK5_At-3xHA
FEATURES             Location/Qualifiers
     source          1..17893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5972..5996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8025..8049)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8078..8167
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      8201..8453
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8882..9134)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9602..10627)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9602..10627)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10637..10981)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10673..10693)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(11123..11140)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11123..11139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11132..11154)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(11279..11298)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11489..11510
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    11757..11781
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(13377..14525)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14824..15618)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15810..16577
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(17270..17893,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgatca ggatattctt gtttaagatg
     4981 ttgaactcta tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc
     5041 atagcgaact tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa
     5101 aaaatattat tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa
     5161 gatccattga tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt
     5221 ttttaacgag acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa
     5281 tcatacaaaa atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg
     5341 ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat
     5401 tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa
     5461 atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc
     5521 caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt
     5581 aaatctcaac ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt
     5641 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
     5701 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
     5761 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
     5821 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
     5881 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
     5941 ctctctaccg tgatcaaggc tagagttatc aacaagtttg tacaaaaaag caggctccgc
     6001 ggccgccccc ttcaccatgg ctgcgtgtac actccacgcg ctctcagtca ccttattcct
     6061 ccttctcttc tttgccgtgt caccggcgaa agctcagcaa ccgtacgtca acaaccacca
     6121 gctcgcctgc gaggtccgtg tctacgacaa cataaccaac ggattcacat gtaacggccc
     6181 accttcttgc cgctcatacc tcactttctg gtctcaacca ccgtacaaca cggcggactc
     6241 aatcgccaaa ctcctcaacg tctccgccgc agagatccaa tcaatcaaca acctccccac
     6301 agccaccacc agaatcccaa cccgtgaatt agtcgtgatc ccagctaact gctcctgctc
     6361 ctcctccagc ggaggatttt accaacacaa cgccacttac aatctctccg gtaacagagg
     6421 agatgaaacc tatttctcag tggctaacga tacttaccaa gctttatcca cgtgtcaagc
     6481 catgatgtca caaaaccgtt acggcgagag acaactaacc cccggcttaa acctccttgt
     6541 tcctctccga tgtgcttgtc ccaccgccaa acaaaccacc gccggattta aatatctcct
     6601 gacttactta gtcgccatgg gagatagtat ctccggcatc gccgagatgt tcaacagcac
     6661 atccgccgcc ataaccgaag gtaacgagct tacatcagac aatatcttct tcttcacacc
     6721 ggttctagtt cctctcacaa ctgaacctac caaaatcgtt atatctccgt cgcctcctcc
     6781 tccacccgtt gttgctacgc cgcctcaaac gccagttgat cctccgggat cttcttcttc
     6841 tcacaaatgg atctacatcg gaattggaat cggagctggt ttgcttctct tactctcaat
     6901 cttagctctc tgcttctaca aacgaaggtc taagaagaag tcattaccgt cgtcgttgcc
     6961 ggaggagaac aagctctttg attcatcaac caaacaatct attcccacaa caacaacgac
     7021 tcaatggtca atagatttat ccaattcatc agaagctttc ggtttaaaat ccgccataga
     7081 atctctaaca ctatacagat tcaacgatct tcaatcagct acttcgaatt tcagcgacga
     7141 aaacagaatc aaaggctctg tttatcgcgc gacaatcaac ggcgacgatg ccgctgtgaa
     7201 agtgatcaaa ggagatgttt cttcctctga gatcaatctt ttgaagaagc taaatcattc
     7261 taatatcatc cgtctctcag gtttctgtat ccgtgaagga acatcgtacc tcgtcttcga
     7321 gtattcagag aatggatcga tcagtgattg gcttcactcg tcgggcaaga agagtttgac
     7381 atggaaacag agagttgaaa tagcaaggga tgtggcagag gcgttagatt atctccataa
     7441 ctatataact ccacctcata ttcataagaa cttggaatca acaaacatac ttctggattc
     7501 taatttcaga gccaagattg cgaatttcgg tgttgcgagg attcttgatg aaggtgatct
     7561 tgatcttcag ttaacaagac atgttgaagg aacacaaggc tacttagctc cagagtatgt
     7621 ggagaatgga gtcattactt cgaaactaga cgtgtttgct tttggagttg cggttcttga
     7681 gcttctttcg gggagagaag cagtaacgat acataagaag aaggaaggag aagaagaagt
     7741 ggagatgttg tgtaaagtga taaacagtgt gcttggagga gagaatgtga gagagaagtt
     7801 aaaagagttt atggatccat ctctagggaa tgagtatccg ttggagctgg cttacaccat
     7861 ggctcagctt gctaagagct gtgtcgcaac tgatcttaac tcgcgtccat ctgtcactca
     7921 ggttctaacc acgctctcaa tgatcgtctc ctcctccatc gattgggagc cttctgatga
     7981 ccttcttcgt tccggctctc ttggcaacaa gggtgggcgc gccgacccag ctttcttgta
     8041 caaagtggtt gataacagcg ggttaattaa catcttttac ccatacgatg ttcctgacta
     8101 tgcgggctat ccctatgacg tcccggacta tgcaggatcc tatccatatg acgttccaga
     8161 ttacgctgct cagtgcagcg cttagagctc gaatttcccc gatcgttcaa acatttggca
     8221 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     8281 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     8341 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     8401 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     8461 agcttcatca acgcaagaca tgcgcacgac cgtctgacag gagaggaatt tccgacgagc
     8521 acagaaagga cttgctcttg gacgtaggcc tatttctcag gcacatgtat caagtgttcg
     8581 gacgtgggtt ttcgatggtg tatcagccgc cgccaactgg gagatgagga ggctttcttg
     8641 gggggcagtc agcagttcat ttcacaagac agaggaactt gtaaggagat gcactgattt
     8701 atcttggcgc aaaccagcag gacgaattag tgggaatagc ccgcgaatat ctaagttatg
     8761 cctgtcggca tgagcagaaa cttccaattc gaaacagttt ggagaggttg tttttgggca
     8821 taccttttgt tagtcagcct ctcgattgct catcgtcatt acacagtacc gaagtttgat
     8881 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     8941 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     9001 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     9061 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     9121 atgtttgaac gatctgcttc gacgcactcc ttctttactc caccatctcg tccttattga
     9181 aaacgtgggt agcaccaaaa cgaatcaagt cgctggaact gaagttacca atcacgctgg
     9241 atgatttgcc agttggatta atcttgcctt tccccgcatg aataatattg atgaatgcat
     9301 gcgtgagggg tatttcgatt ttggcaatag ctgcaattgc cgcgacatcc tccaacgagc
     9361 ataattcttc agaaaaatag cgatgttcca tgttgtcagg gcatgcatga tgcacgttat
     9421 gaggtgacgg tgctaggcag tattccctca aagtttcata gtcagtatca tattcatcat
     9481 tgcattcctg caagagagaa ttgagacgca atccacacgc tgcggcaacc ttccggcgtt
     9541 cgtggtctat ttgctcttgg acgttgcaaa cgtaagtgtt ggatcccggt cggcatctac
     9601 tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
     9661 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
     9721 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
     9781 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
     9841 ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
     9901 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
     9961 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
    10021 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
    10081 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
    10141 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
    10201 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag
    10261 cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca
    10321 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga
    10381 attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa
    10441 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc
    10501 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg
    10561 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct
    10621 ttttcatatc ggggtcgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc
    10681 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
    10741 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
    10801 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
    10861 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
    10921 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
    10981 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
    11041 gtgctccacc atgttgacgg atctctagga cgcgtcgacg cgtcgacgcg tcgacgcgtc
    11101 gacgcgtcct agaagctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    11161 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
    11221 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgcccgct
    11281 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    11341 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    11401 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    11461 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    11521 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggaaccacca
    11581 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
    11641 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
    11701 ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
    11761 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
    11821 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
    11881 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
    11941 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
    12001 gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta tcagccttct
    12061 tattcatttc tcgcttaacc gtgacaggct gtcgatcttg agaactatgc cgacataata
    12121 ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg ctatttcttt agaagtgaac
    12181 gttgacgata tcaactcccc tatccattgc tcaccgaatg gtacaggtcg gggacccgaa
    12241 gttccgactg tcggcctgat gcatccccgg ctgatcgacc ccagatctgg ggctgagaaa
    12301 gcccagtaag gaaacaactg taggttcgag tcgcgagatc ccccggaacc aaaggaagta
    12361 ggttaaaccc gctccgatca ggccgagcca cgccaggccg agaacattgg ttcctgtagg
    12421 catcgggatt ggcggatcaa acactaaagc tactggaacg agcagaagtc ctccggccgc
    12481 cagttgccag gcggtaaagg tgagcagagg cacgggaggt tgccacttgc gggtcagcac
    12541 ggttccgaac gccatggaaa ccgcccccgc caggcccgct gcgacgccga caggatctag
    12601 cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca gttccgcaaa tagcccccag
    12661 gaccgccatc aatcgtatcg ggctacctag cagagcggca gagatgaaca cgaccatcag
    12721 cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa
    12781 caacaagctc cagaatagcg aaatattaag tgcgccgagg atgaagatgc gcatccacca
    12841 gattcccgtt ggaatctgtc ggacgatcat cacgagcaat aaacccgccg gcaacgcccg
    12901 cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag
    12961 atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg aagaccgaca gcccaatgat
    13021 ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc
    13081 catgggcttt ttctcctcgt gctcgtaaac ggacccgaac atctctggag ctttcttcag
    13141 ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc gctccaagcc gtcgaatctg
    13201 agccttaatc acaattgtca attttaatcc tctgtttatc ggcagttcgt agagcgcgcc
    13261 gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga
    13321 aatgccagta aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag
    13381 cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc
    13441 aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat
    13501 ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa
    13561 tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc
    13621 gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg
    13681 gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg
    13741 tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc
    13801 tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag
    13861 aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc
    13921 cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc
    13981 ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc
    14041 gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac
    14101 ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc
    14161 aacgcggcct gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc
    14221 ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc
    14281 tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga
    14341 gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg
    14401 gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc
    14461 tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga
    14521 ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc
    14581 tgatttgacc cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg
    14641 gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg
    14701 aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga gccaaaacac
    14761 ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag
    14821 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    14881 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
    14941 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    15001 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    15061 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    15121 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    15181 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    15241 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    15301 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    15361 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
    15421 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    15481 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    15541 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    15601 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    15661 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    15721 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    15781 acggagccga ttttgaaacc acaattatgg gtgatgctgc caacttactg atttagtgta
    15841 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
    15901 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc
    15961 tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg
    16021 caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac agcccgcatt
    16081 atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg cagtcggtaa
    16141 cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg
    16201 gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga
    16261 agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga
    16321 gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat
    16381 cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc
    16441 ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa
    16501 aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat
    16561 aagttggagt cattacccaa ttatgataga atttacaagc tataaggtta ttgtcctggg
    16621 tttcaagcat tagtccatgc aagtttttat gctttgccca ttctatagat atattgataa
    16681 gcgcgctgcc tatgccttgc cccctgaaat ccttacatac ggcgatatct tctatataaa
    16741 agatatatta tcttatcagt attgtcaata tattcaaggc aatctgcctc ctcatcctct
    16801 tcatcctctt cgtcttggta gctttttaaa tatggcgctt catagagtaa ttctgtaaag
    16861 gtccaattct cgttttcata cctcggtata atcttaccta tcacctcaaa tggttcgctg
    16921 ggtttatcgc acccccgaac acgagcacgg cacccgcgac cactatgcca agaatgccca
    16981 aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc cccgacggcc gaagtgaagg
    17041 gcaggccgcc acccaggccg ccgccctcac tgcccggcac ctggtcgctg aatgtcgatg
    17101 ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct cgggctgatc gcccatcccg
    17161 ttactgcccc gatcccggca atggcaagga ctgccagcgc tgccattttt ggggtgaggc
    17221 cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg
    17281 agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag
    17341 ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag
    17401 cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc
    17461 ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag
    17521 gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt
    17581 atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg
    17641 atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc
    17701 aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg
    17761 agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc
    17821 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg
    17881 gcaaccagcc cgg
//
