LOCUS       sequence-399753-        9059 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector that expresses a scrambled control shRNA for 
            SVA-lncRNA AK057321 shRNA 2S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson 
            M, Moreno A, Matthew P Anderson
  TITLE     Hominoid SVA-lncRNA AK057321 targets human-specific SVA 
            retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal 
            maturation.
  JOURNAL   Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8.
  PUBMED    36997626
REFERENCE   2  (bases 1 to 9059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-03-30"; volume: "6(1):347. doi"; pages: " 
            10.1038/s42003-023-04683-8"
COMMENT     Sequence Label: SVA-lncRNA AK057321 shRNA 2S scramble
FEATURES             Location/Qualifiers
     source          1..9059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     458..477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(696..800)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    848..982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(885..904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     939..958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1150..1783
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1830..1955
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2452..2685
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2870..2914
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3177..3292
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        3345..3593
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3345..3365
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3516..3535
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3685..4896
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4733..4752
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4903..4959
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4972..5343
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             5353..5406
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5428..5437
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5434..6144
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5579..5597)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5847..5866
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6177..6765
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6230..6250)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6648..6659)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6972..7605
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7733..7749)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7733..7749)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7746..7768)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7757..7773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7781..7787,7788..7805,7806..7811))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7826..7847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7964..7981)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8135..8723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8215..8234)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8894..9059,1..626,627..695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
       61 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
      301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
      361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
      421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
      481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
      541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
      601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
      661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
      721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
      841 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      961 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     1021 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     1081 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     1141 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     1201 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     1261 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     1321 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     1381 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     1441 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     1501 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     1561 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     1621 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1681 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1741 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1801 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     1861 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1921 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1981 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     2041 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     2101 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     2161 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     2221 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     2281 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     2341 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2401 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2461 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     2521 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     2581 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2641 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     2701 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     2761 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2821 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2881 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2941 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3001 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3061 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3121 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta
     3181 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     3241 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     3301 attacaggga cagcagagat ccagtttatc gatctgggca ggaagagggc ctatttccca
     3361 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     3421 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     3481 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     3541 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagga tccgtccgac
     3601 cacgtatcat cattcaagag atgatgatac gtgtcggact tttttacgcg tgaattcgaa
     3661 ttaacccgtg tcggctccag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg
     3721 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     3781 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     3841 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     3901 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     3961 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     4021 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     4081 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     4141 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     4201 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     4261 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     4321 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     4381 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     4441 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     4501 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     4561 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     4621 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     4681 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     4741 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     4801 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     4861 gctaaattct ggccgttttt ggcttttttg ttagactctg gcgccacgaa tttctcgcta
     4921 ctcaagcagg ccggtgatgt cgaggaaaac cctggtccta tggtgtctag aatggccaag
     4981 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     5041 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     5101 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     5161 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     5221 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     5281 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     5341 gacggttcag gggagggaag aggttccctg ctcacatgcg gagacgtaga agagaatccc
     5401 ggacccatga cctctagaga accggtcgcc accatggtga gcaagggcga ggaggataac
     5461 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     5521 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     5581 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     5641 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     5701 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     5761 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     5821 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     5881 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     5941 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     6001 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     6061 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     6121 ggcatggacg agctgtacaa gtagctcgag tctagagggc ccgacgcgtc tggaacaatc
     6181 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6241 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6301 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6361 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6421 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6481 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6541 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     6601 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6661 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6721 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     6781 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     6841 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     6901 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6961 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     7021 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     7081 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     7141 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     7201 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     7261 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     7321 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     7381 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     7441 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7501 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7561 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7621 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     7681 gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7741 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7801 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7861 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7921 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7981 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8401 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8461 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8521 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8581 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8641 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8701 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8761 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8821 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8881 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8941 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9001 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagat
//
