LOCUS       sequence-400020-        4765 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Complementation of M. smegmatis MceG(WT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2759
FEATURES             Location/Qualifiers
     source          1..4765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1335..1381
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        1420..1467
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1486..1860
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      1912..2500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2401..2420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cactcgcgac cggtgagtgc taggtcggga cggtgaggcc aggcccgtcg tcgcagcgag
       61 tggcagcgag gacaacttga gccgtccgtc gcgggcactg cgcccggcca gcgtaagtag
      121 cggggttgcc gtcacccggt gacccccgtt tcatccccga tccggaggaa tcacttcgca
      181 atgggcgtcc aaatcgacgt gacggggttg agcaagtctt tcgggtcgtc aaagatctgg
      241 gaagacgtga ccatgtcgat cccggccggc gaggtgagcg tgctgctggg cccctccggt
      301 accggcaaat ccgtgttcct gaagtcgctg atcggtttgc tccgccccga acgtggctcc
      361 atcgtcatcg acggcaccga catcctgcag tgctcggcca aggagctcta cgagatccgc
      421 acgctgttcg gtgtgctgtt ccaggacggc gcgctgttcg gctcgatgaa catctacgac
      481 aacaccgcgt tcccgctccg tgagcacacc aagaagagcg agagcgaaat ccgcaagatc
      541 gtgatggaga agctcgacct ggtcggtatg cccaacgacg ggcacaagtt ccccggtgag
      601 atctccggcg gtatgcgcaa gcgcgccggt ctggcccgcg cactcgtgct cgatccggag
      661 atcatcctct gcgacgagcc ggactcgggt ctggacccgg tgcgtacggc ctacctgtcg
      721 cagctgctga tcgacatcaa cgcgcagatc gacgcgaccg tgctgatcgt gacgcacaac
      781 atcaacatcg cccgcaccgt gcccgacaac atgggcatgc tgttccgcaa gcagctggtg
      841 atgttcggcc cgcgtgaggt gcttctgacc agcgaggagc cggtggtcaa gcagttcctc
      901 aacggccgcc gtatcggccc gatcggtatg tcggaggaga aggacgaggc caccgcggcc
      961 gccgagcagg ccctggtgga cgccggccag cacgacggcg gtgtcgagga gatcgagggt
     1021 gtgccgccgc agctgcaggc caccccgggt atgcccgagc gcaaggccgt ggcccgccgc
     1081 aaggcccgcg tgcgcgagat cctgcacacc ctcccgcccg ccgcgcaggc cgcgatcctc
     1141 gaagagctcg accgcgacca gccgcagctg tccgccccga ccacccagac ggccgccacc
     1201 gcgcccgtcg agaactacga cgactcgccg acgggcgtca tcgaggtgcc caagcaggcg
     1261 taagatccag ctgcagaatt cgaagcttat cgatgtcgac gtagttaact agcgtacgat
     1321 cgactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1381 tgccatcatg gccgcggtga tcagctagct cgagcacgtg ttgacaatta atcatcggca
     1441 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc taagttgacc
     1501 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     1561 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     1621 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     1681 gtgtgggtgc gcggcctgga cgagctgtac accgagtggt cggaggtcgt gtccacgaac
     1741 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     1801 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     1861 gaatctagtt ccatgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1921 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1981 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2041 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2101 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2161 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2221 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2281 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgagg ggagaaaggc
     2341 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2401 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2461 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2521 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2581 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2641 aacgaccgag cgcaacgcgt gagcccacca gctccgtaag ttcgggcgct gtgtggctcg
     2701 tacccgcgca ttcaggcggc agggggtcta acgggtctaa ggcggcgtgt acggccgcca
     2761 cagcggctct cagcggcccg gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg
     2821 tacaggtggt tgggggtgct cggctgtcgc tggtgttcca ccaccagggc tcgacgggag
     2881 agcgggggag tgtgcagttg tggggtggcc cctcagcgaa atatctgact tggagctcgt
     2941 gtcggaccat acaccggtga ttaatcgtgg tctactacca agcgtgagcc acgtcgccga
     3001 cgaatttgag cagctctggc tgccgtactg gccgctggca agcgacgatc tgctcgaggg
     3061 gatctaccgc caaagccgcg cgtcggccct aggccgccgg tacatcgagg cgaacccaac
     3121 agcgctggca aacctgctgg tcgtggacgt agaccatcca gacgcagcgc tccgagcgct
     3181 cagcgcccgg gggtcccatc cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca
     3241 cgcacacgca gtgtgggcac tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa
     3301 gccgctcgca tacatggcgg cgtgcgccga aggccttcgg cgcgccgtcg atggcgaccg
     3361 cagttactca ggcctcatga ccaaaaaccc cggccacatc gcctgggaaa cggaatggct
     3421 ccactcagat ctctacacac tcagccacat cgaggccgag ctcggcgcga acatgccacc
     3481 gccgcgctgg cgtcagcaga ccacgtacaa agcggctccg acgccgctag ggcggaattg
     3541 cgcactgttc gattccgtca ggttgtgggc ctatcgtccc gccctcatgc ggatctacct
     3601 gccgacccgg aacgtggacg gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa
     3661 cgccgaattt ccgtgcaacg acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc
     3721 catcgccaac agcatttggc gttggatcac aaccaagtcg cgcatttggg cggacgggat
     3781 cgtggtctac gaggccacac tcagtgcgcg ccagtcggcc atctcgcgga agggcgcagc
     3841 agcgcgcacg gcggcgagca cagttgcgcg gcgcgcaaag tccgcgtcag ccatggaggc
     3901 attgctatga gcgacggcta cagcgacggc tacaaccggc agccgactgt ccgcaaaaag
     3961 cggcgcgtga ccgccgccga aggcgctcga atcaccggac tatccgaacg ccacgtcgtc
     4021 cggctcgtgg cgcaggaacg cagcgagtgg ctcgccgagc aggctgcacg ccgcgaacgc
     4081 atccgcgcct atcacgacga cgagggccac tcttggccgc aaacggccaa acatttcggg
     4141 ctgcatctgg acaccgttaa gcgactcggc tatcgggcga ggaaagagcg tgcggcagaa
     4201 caggaagcgg ctcaaaaggc ccacaacgaa gccgacaatc caccgctgtt ctaacgcaat
     4261 tggggagcgg gtgtcgcggg ggttccgtgg ggggttccgt tgcaacgggt cggacaggta
     4321 aaagtcctgg tagacgctag ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg
     4381 ttgcgcccgt tttgaatacc agccagacga gacggggttc tacgaatctt ggtcgatacc
     4441 aagccatttc cgctgaatat cggggagctc accgccagaa tcggtggttg tggtgatgta
     4501 cgtggcgaac tccgttgtag tgcctgtggt ggcatccgtg gcgcggccgc ggtaccagat
     4561 ctttaaatct agacggtgac cacaacgcgc ccgctttgat cggggacgtc tgcggccgac
     4621 catttacggg tcttgttgtc gttggcggtc atgggccgaa catactcacc cggatcggag
     4681 ggccgaggac aaggtcgaac gaggggcatg acccggtgcg gggcttcttg cactcggcat
     4741 aggcgagtgc taagaataac gttgg
//
