LOCUS       sequence-400563-       10579 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1839
FEATURES             Location/Qualifiers
     source          1..10579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6436..7024
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6489..6509)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6907..6918)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7096..7329
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7407..7528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7444..7463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7498..7517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7568..7703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7723..7742)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7724..7742)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7752..7769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7752..7768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7761..7783)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7910..8365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7997..8016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8207..8228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8391..8495
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8496..8564,8565..9356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8714..8733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9527..10115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10016..10035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10269..10286
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10403..10424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10439..10444,10445..10462,10463..10469)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10477..10493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10482..10504
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10501..10517
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10501..10517
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10536..10556
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10538..10556
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgggc cagactggga agaaatctga
     4141 gaagggacca gtttgttggc ggaagcgtgt aaaatcagag tacatgcgac tgagacagct
     4201 caagaggttc agacgagctg atgaagtaaa gagtatgttt agttccaatc gtcagaaaat
     4261 tttggaaaga acggaaatct taaaccaaga atggaaacag cgaaggatac agcctgtgca
     4321 catcctgact tcttgttcgg tgaccagtga cttggatttt ccaacacaag tcatcccatt
     4381 aaagactctg aatgcagttg cttcagtacc cataatgtat tcttggtctc ccctacagca
     4441 gaattttatg gtggaagatg aaactgtttt acataacatt ccttatatgg gagatgaagt
     4501 tttagatcag gatggtactt tcattgaaga actaataaaa aattatgatg ggaaagtaca
     4561 cggggataga gaatgtgggt ttataaatga tgaaattttt gtggagttgg tgaatgccct
     4621 tggtcaatat aatgatgatg acgatgatga tgatggagac gatcctgaag aaagagaaga
     4681 aaagcagaaa gatctggagg atcaccgaga tgataaagaa agccgcccac ctcggaaatt
     4741 tccttctgat aaaatttttg aagccatttc ctcaatgttt ccagataagg gcacagcaga
     4801 agaactaaag gaaaaatata aagaactcac cgaacagcag ctcccaggcg cacttcctcc
     4861 tgaatgtacc cccaacatag atggaccaaa tgctaaatct gttcagagag agcaaagctt
     4921 acactccttt catacgcttt tctgtaggcg atgttttaaa tatgactgct tcctacatcc
     4981 ttttcatgca acacccaaca cttataagcg gaagaacaca gaaacagctc tagacaacaa
     5041 accttgtgga ccacagtgtt accagcattt ggagggagca aaggagtttg ctgctgctct
     5101 caccgctgag cggataaaga ccccaccaaa acgtccagga ggccgcagaa gaggacggct
     5161 tcccaataac agtagcaggc ccagcacccc caccattaat gtgctggaat caaaggatac
     5221 agacagtgat agggaagcag ggactgaaac ggggggagag aacaatgata aagaagaaga
     5281 agagaagaaa gatgaaactt cgagctcctc tgaagcaaat tctcggtgtc aaacaccaat
     5341 aaagatgaag ccaaatattg aacctcctga gaatgtggag tggagtggtg ctgaagcctc
     5401 aatgtttaga gtcctcattg gcacttacta tgacaatttc tgtgccattg ctaggttaat
     5461 tgggaccaaa acatgtagac aggtgtatga gtttagagtc aaagaatcta gcatcatagc
     5521 tccagctccc gctgaggatg tggatactcc tccaaggaaa aagaagagga aacaccggtt
     5581 gtgggctgca cactgcagaa agatacagct gaaaaaggac ggctcctcta accatgttta
     5641 caactatcaa ccctgtgatc atccacggca gccttgtgac agttcgtgcc cttgtgtgat
     5701 agcacaaaat ttttgtgaaa agttttgtca atgtagttca gagtgtcaaa accgctttcc
     5761 gggatgccgc tgcaaagcac agtgcaacac caagcagtgc ccgtgctacc tggctgtccg
     5821 agagtgtgac cctgacctct gtcttacttg tggagccgct gaccattggg acagtaaaaa
     5881 tgtgtcctgc aagaactgca gtattcagcg gggctccaaa aagcatctat tgctggcacc
     5941 atctgacgtg gcaggctggg ggatttttat caaagatcct gtgcagaaaa atgaattcat
     6001 ctcagaatac tgtggagaga ttatttctca agatgaagct gacagaagag ggaaagtgta
     6061 tgataaatac atgtgcagct ttctgttcaa cttgaacaat gattttgtgg tggatgcaac
     6121 ccgcaagggt aacaaaattc gttttgcaaa tcattcggta aatccaaact gctatgcaaa
     6181 agttatgatg gttaacggtg atcacaggat aggtattttt gccaagagag ccatccagac
     6241 tggcgaagag ctgttttttg attacagata cagccaggct gatgccctga agtatgtcgg
     6301 catcgaaaga gaaatggaaa tcccttagta atgaactagt ccgtaacttg aaagtatttc
     6361 gatttcttgg ctttatatat cttgtggaaa ggacgagttc aacgcaggga cacgacgcgg
     6421 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6481 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6541 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6601 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6661 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6721 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6781 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6841 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6901 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6961 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7021 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7081 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     7141 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7201 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7261 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7321 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7381 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7501 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7561 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7621 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7681 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     7741 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7801 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7861 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7921 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7981 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8041 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8101 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8161 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8221 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8281 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8341 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8401 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8461 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8521 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8581 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8641 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8701 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8761 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8821 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8881 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8941 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9001 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9061 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9121 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9181 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9241 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9301 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9361 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9421 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9601 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9661 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9721 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9781 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9841 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9901 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9961 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10021 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10081 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10141 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10201 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10261 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10321 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10381 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10441 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10501 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    10561 aaaagctgga gctgcaagc
//
