LOCUS       sequence-400837-       10938 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Encodes wild type Gal4-VP64 transcription factor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Zhu L, McNamara HM, Toettcher JE
  TITLE     Light-switchable transcription factors obtained by direct screening 
            in mammalian cells.
  JOURNAL   Nat Commun. 2023 Jun 2;14(1):3185. doi: 10.1038/s41467-023-38993-6.
  PUBMED    37268649
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-2"; volume: "14(1):3185. doi"; pages: " 
            10.1038/s41467-023-38993-6"
COMMENT     Sequence Label: pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)
FEATURES             Location/Qualifiers
     source          1..10938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             249..293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    578..695
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        848..1255
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1808..1957
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    1983..2556
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2006..2558
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2150..2167)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2377..2396
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2558..3268
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2703..2721)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2971..2990
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3330..3918
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3383..3403)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3801..3812)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3921..3937)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3922..3938)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4028..4261
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4286..4304)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4287..4304)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4594..4613)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4827..4846)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4946..4968
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5006..5024)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5092..5196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5197..5265,5266..6057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5415..5434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6228..6816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6717..6736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6970..6987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7057..7077)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7062..7391
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7242..7377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8525..8590
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8720..8740
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9165..9299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9202..9221)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9256..9275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9467..10100
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10147..10272
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(10769..10938,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      601 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      661 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      721 atccagtttg gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat
      781 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
      841 agctgcagta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
      901 cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg
      961 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     1021 ccgaggccaa gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     1081 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     1141 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     1201 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     1261 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc
     1321 ggtcgccacc atgaagctgc tgagcagcat cgagcaggcc tgtgacatct gccggctgaa
     1381 gaaactgaag tgcagcaaag aaaagcccaa gtgcgccaag tgcctgaaga acaactggga
     1441 gtgccggtac agccccaaga ccaagagaag ccccctgacc agagcccacc tgaccgaggt
     1501 ggaaagccgg ctggaaagac tggaacagct gtttctgctg atcttcccac gcgaggacct
     1561 ggacatgatc ctgaagatgg acagcctgca ggacatcaag gccctgctga ccggcctgtt
     1621 cgtgcaggac aacgtgaaca aggacgccgt gaccgacaga ctggccagcg tggaaaccga
     1681 catgcccctg accctgcggc agcacagaat cagcgccacc agcagcagcg aggaaagcag
     1741 caacaagggc cagcggcagc tgacagtgtc tgctgctgca ggcggaagcg gaggctctgg
     1801 cggatctgat gccctggacg acttcgacct ggatatgctg ggcagcgacg ccctggatga
     1861 ttttgatctg gacatgctgg gatctgacgc tctggacgat ttcgatctcg acatgttggg
     1921 atcagatgca ctggatgact ttgacctgga catgctcgga tcatgagtac caagctgatc
     1981 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     2041 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     2101 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     2161 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     2221 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     2281 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     2341 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     2401 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     2461 tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     2521 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gtgagcaagg gcgaggagga
     2581 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     2641 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     2701 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     2761 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     2821 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2881 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     2941 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     3001 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     3061 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     3121 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     3181 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     3241 cggcggcatg gacgagctgt acaagtagct gaggccgcga ctctagagtc gacctgcagg
     3301 catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3361 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3421 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3481 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3541 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3601 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3661 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3721 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3781 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3841 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3901 tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta
     3961 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4021 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4081 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4141 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4201 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4261 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     4321 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     4381 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     4441 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     4501 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     4561 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     4621 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     4681 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     4741 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     4801 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4861 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4921 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4981 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5041 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5161 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5221 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5281 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5341 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5401 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5461 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5521 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5581 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5641 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5701 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5761 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5821 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5881 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5941 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6001 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6061 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6121 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6181 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6241 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6301 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6361 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6421 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6481 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6541 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6601 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6661 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6721 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6781 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6841 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6901 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6961 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7021 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
     7081 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7141 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7201 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7261 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7321 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7381 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
     7441 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     7501 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     7561 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     7621 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     7681 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
     7741 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     7801 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     7861 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     7921 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     7981 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     8041 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     8101 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     8161 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     8221 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     8281 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
     8341 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     8401 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
     8461 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     8521 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     8581 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     8641 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     8701 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     8761 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     8821 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     8881 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     8941 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     9001 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     9061 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     9121 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     9181 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9241 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     9301 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     9361 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     9421 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     9481 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9541 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     9601 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     9661 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     9721 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     9781 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     9841 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     9901 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10081 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10141 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10201 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10261 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10321 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10381 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10441 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10501 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10561 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    10621 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    10681 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    10741 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    10801 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    10861 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10921 tgcaactcac agtctggg
//
