LOCUS       sequence-401008-        8648 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Fluorescent reporter for genetic tracing of epithelial cell 
            SARS-CoV2 response..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, 
            Serresi M, Cicin-Sain L, Gargiulo G
  TITLE     Pharmacological modulators of epithelial immunity uncovered by 
            synthetic genetic tracing of SARS-CoV-2 infection responses.
  JOURNAL   Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. 
            Epub 2023 Jun 21.
  PUBMED    37343108
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-23"; volume: "9(25):eadf4975. doi"; pages: " 
            10.1126/sciadv.adf4975. Epub 2023 Jun 21"
COMMENT     Sequence Label: pPB-NeoCOVGT3-d2EGFP-mCherry
FEATURES             Location/Qualifiers
     source          1..8648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1094..1118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1119..1128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1125..1127,1128..1130,1131..1841,1842..1847,
                     1848..1970)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1170..1191)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1431..1450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1778..1799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1974..1983
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2125..2143)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             2706..2726
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2730..2750
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2754..2774
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2913..2937)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2941..2965)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3043..3062)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3108..3163
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3162..3181)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        3518..3897
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3898..4101
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4052..4072
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4134..4928
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4134..4928
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4188..4207)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4798..4817
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4966..4983)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4972..5196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5546..5608
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5674..5693)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5675..5693)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5700..5717)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5700..5716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5709..5731)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5858..6313
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5945..5964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6155..6176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6339..6443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6444..6512,6513..7304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6662..6681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7475..8063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7964..7983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8217..8234
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8351..8372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8387..8392,8393..8410,8411..8417)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8425..8441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8430..8452
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8449..8465
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8449..8465
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8486..8504
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttggagt tgtgatcctt tggaggagaa
      361 gaggcattgt ggtttttgga attttcagca tttttgtgct ggtttttcct catctttgtg
      421 gatttatcta cctttcatct ttgatgttgg tgacctttgg atggggtttt tgcatgggca
      481 tcctttccta ctaggtttca ttttaatcct tttcaccagt tcccaaatgt aagtttcttt
      541 ttcctttctc ttttgaggca ggagatcctg ctgaactatg tattttccca ttgttatgca
      601 acttttcatt tgagttttta agaagtagta acaatatgca aaccccagtc agctaatttg
      661 ctttttgttt ttatggtttt gcctactcta gatatttcct ataaaatgga atcatacaat
      721 atgtggtccc ttgtgtctgg ctttttttac ttagcatatt ttcaaggttc atccatgttg
      781 tagcatgtat tcttccctct ggatggatac gcacatacac tggatacaca cacacacaca
      841 cacacacaca cacacacaca cacacacatt tcgcctaaca agttctatat cagactatat
      901 caggttctcc cccccgcccc ctccatgtgt cagggactat acactactgc aaataggaag
      961 ggacagtaac atttagaaga gaacaaaaca gaaactcttg gaagcaggaa aggtgcatga
     1021 ctcaaagagg gaaattcctg tgccataaaa ggattgctgg tgtataaaat gctctatata
     1081 tgccaattat ctaacaagtt tgtacaaaaa agcaggctgc caccatggtg agcaagggcg
     1141 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1201 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1261 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1321 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1381 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1441 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1501 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1561 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1621 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1681 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1741 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1801 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaagcttagc catggcttcc
     1861 cgccggaggt ggaggagcag gatgatggca cgctgcccat gtcttgtgcc caggagagcg
     1921 ggatggaccg tcaccctgca gcctgtgctt ctgctaggat caatgtgtag actgccacca
     1981 tggtgagcaa gggcgaggag gacaacatgg ccatcatcaa ggagttcatg aggttcaagg
     2041 tgcacatgga gggcagcgtg aacggccacg agttcgagat cgagggcgag ggcgagggca
     2101 ggccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     2161 tcgcctggga catcctgagc ccccagttca tgtacggcag caaggcctac gtgaagcacc
     2221 ccgccgacat ccccgactac ctgaagctga gcttccccga gggcttcaac tgggagaggg
     2281 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggacagcagc ctgcaggacg
     2341 gcgagttcat ctacaaggtg aagctgaggg gcaccaactt ccccagcgac ggccccgtga
     2401 tgcagtgcag gaccatgggc tgggaggcca gcaccgagag gatgtacccc gaggacggcg
     2461 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgccg
     2521 aggtgaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtgg
     2581 acatcaagct ggacatcctg agccacaacg aggactacac catcgtggag cagtacgaga
     2641 gggccgaggg caggcacagc accggcggca tggacgagct gtacaagctc agatctcgag
     2701 ctgatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa
     2761 agaagagaaa ggtaggatcc accggatcta gccatggctt cccgccggag gtggaggagc
     2821 aggatgatgg cacgctgccc atgtcttgtg cccaggagag cgggatggac cgtcaccctg
     2881 cagcctgtgc ttctgctagg atcaatgtgt agacccagct ttcttgtaca aagtggtgat
     2941 acccagcttt cttgtacaaa gtggtgatcc tcaggtgcag gctgcctatc agaaggtggt
     3001 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     3061 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     3121 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     3181 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     3241 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     3301 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     3361 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     3421 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     3481 atggagatcc ctcgacctgc agcccaagct tcgcgttgac attgattatt gactagttat
     3541 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3601 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3661 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3721 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3781 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3841 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3901 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     3961 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     4021 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     4081 gaggtctata taagcagagc tctctggcta actagagaac ccactgcgcc accatgattg
     4141 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4201 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4261 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     4321 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4381 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4441 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4501 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4561 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4621 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4681 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4741 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4801 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4861 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4921 tcttctgact cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     4981 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5041 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5101 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5161 atagcaggca tgctggggat gcggtgggct ctatggctcg agttaattaa cgagagcata
     5221 atattgatat gtgccaaagt tgtttctgac tgactaataa gtataatttg tttctattat
     5281 gtataggtta agctaattac ttattttata atacaacatg actgttttta aagtacaaaa
     5341 taagtttatt tttgtaaaag agagaatgtt taaaagtttt gttactttat agaagaaatt
     5401 ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt gttgaattta
     5461 ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa
     5521 cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta tctttaacgt
     5581 acgtcacaat atgattatct ttctagggtt aaataatagt ttctaatttt tttattattc
     5641 agcctgctgt cgtgaatacc gagctccaat tcgccctata gtgagtcgta ttacaattca
     5701 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5761 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5821 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     5881 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5941 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6001 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6061 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6121 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6181 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6241 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6301 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6361 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6421 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6481 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6541 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6601 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6661 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6721 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6781 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6841 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6901 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6961 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7021 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7081 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7141 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7201 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7261 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7621 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8161 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8221 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8281 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8341 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8401 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8461 atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggtac
     8521 ctcgcgcgac ttggtttgcc attctttagc gcgcgtcgcg tcacacagct tggccacaat
     8581 gtggtttttg tcaaacgaag attctatgac gtgtttaaag tttaggtcga gtaaagcgca
     8641 aatctttt
//
