LOCUS       sequence-401520-        2429 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Entry vector for Gateway with Eps8L2_494-546.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, 
            Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
  TITLE     Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear 
            positioning during cell migration.
  JOURNAL   Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 
            10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.
  PUBMED    33567288
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.01.007"; journalName: "Curr Biol"; date: 
            "2021-04-12- 12"; volume: "31"; issue: "7"; pages: "1521-1530.e8"
COMMENT     Sequence Label: pDONR201-Eps8L2_494-546
FEATURES             Location/Qualifiers
     source          1..2429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      116..202
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      294..321
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(456..1044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(536..555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1187..1996)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1910..1929
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1988..2007
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2119..2218
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(join(2393..2429,1..63))
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
       61 ttggggcccg agatccatgc tagcgttaac gcgagagtag ggaactgcca ggcatcaaat
      121 aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      181 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc
      241 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc
      301 catcctgacg gatggccttt ttgcgtttct acaaactctt cctggctagc ggtaatacgg
      361 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gcttgcgccg tcccgtcaag
     1141 tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat
     1201 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     1261 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     1321 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     1381 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     1441 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     1501 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     1561 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     1621 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     1681 ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     1741 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     1801 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     1861 cgggcttccc atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     1921 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc
     1981 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     2041 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc
     2101 agagctgcag ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg
     2161 caacaaattg ataagcaatg ctttcttata atgccaactt tgtacaagaa agctgggtct
     2221 aggatgttgc agggcacgta ccccgcctgg ccgctgcggc tgcgcagctt ccaccactgc
     2281 cggccgtcct ccagcacctc taggacctca tccttgagca ccgatagctc gttggcattt
     2341 cgggctgtga agtcatagag aattttcaca tatttggcca tggctggcat ggagcctgct
     2401 tttttgtaca aagttggcat tataaaaaa
//
