LOCUS       sequence-401598-        9442 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization 
            signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 
            triplex, dCas12a direct repeats (DR), and a crRNA cloning site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9442)
  TITLE     SGRN: A Cas12a-driven Synthetic Gene Regulatory Network System
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 
            pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA
FEATURES             Location/Qualifiers
     source          1..9442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   14..143
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        246..1424
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          477..1415
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1372..1392
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1459..5379
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLANLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5099..5108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5416..5436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5443..5463
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5470..5490
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5530..5679
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5689..5769
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     5966..5982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5966..5982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    6043..6631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6096..6116)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6514..6525)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   6718..6858
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6718..6847
                     /label=AAV2 ITR
     rep_origin      6933..7388
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7020..7039)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7230..7251
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7405..7424)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7524..7546
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7584..7602)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7670..7774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7775..7843,7844..8635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7993..8012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8806..9394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9295..9314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggcg
       61 tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc
      121 caactccatc actaggggtt cctgcggccg cacgcgttta attaatgcaa agatggataa
      181 agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg
      241 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
      301 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      361 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      421 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      481 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      541 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
      601 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
      661 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
      721 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
      781 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
      841 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
      901 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
      961 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     1021 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     1081 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     1141 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     1201 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     1261 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     1321 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     1381 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagctagc gtttaaactt
     1441 aagcttggta ccgccaccat gacacagttc gagggcttta ccaacctgta tcaggtgagc
     1501 aagacactgc ggtttgagct gatcccacag ggcaagaccc tgaagcacat ccaggagcag
     1561 ggcttcatcg aggaggacaa ggcccgcaat gatcactaca aggagctgaa gcccatcatc
     1621 gatcggatct acaagaccta tgccgaccag tgcctgcagc tggtgcagct ggattgggag
     1681 aacctgagcg ccgccatcga ctcctataga aaggagaaaa ccgaggagac aaggaacgcc
     1741 ctgatcgagg agcaggccac atatcgcaat gccatccacg actacttcat cggccggaca
     1801 gacaacctga ccgatgccat caataagaga cacgccgaga tctacaaggg cctgttcaag
     1861 gccgagctgt ttaatggcaa ggtgctgaag cagctgggca ccgtgaccac aaccgagcac
     1921 gagaacgccc tgctgcggag cttcgacaag tttacaacct acttctccgg cttttatgag
     1981 aacaggaaga acgtgttcag cgccgaggat atcagcacag ccatcccaca ccgcatcgtg
     2041 caggacaact tccccaagtt taaggagaat tgtcacatct tcacacgcct gatcaccgcc
     2101 gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg ccatcggcat cttcgtgagc
     2161 acctccatcg aggaggtgtt ttccttccct ttttataacc agctgctgac acagacccag
     2221 atcgacctgt ataaccagct gctgggagga atctctcggg aggcaggcac cgagaagatc
     2281 aagggcctga acgaggtgct gaatctggcc atccagaaga atgatgagac agcccacatc
     2341 atcgcctccc tgccacacag attcatcccc ctgtttaagc agatcctgtc cgataggaac
     2401 accctgtctt tcatcctgga ggagtttaag agcgacgagg aagtgatcca gtccttctgc
     2461 aagtacaaga cactgctgag aaacgagaac gtgctggaga cagccgaggc cctgtttaac
     2521 gagctgaaca gcatcgacct gacacacatc ttcatcagcc acaagaagct ggagacaatc
     2581 agcagcgccc tgtgcgacca ctgggataca ctgaggaatg ccctgtatga gcggagaatc
     2641 tccgagctga caggcaagat caccaagtct gccaaggaga aggtgcagcg cagcctgaag
     2701 cacgaggata tcaacctgca ggagatcatc tctgccgcag gcaaggagct gagcgaggcc
     2761 ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg ccgccctgga tcagccactg
     2821 cctacaaccc tgaagaagca ggaggagaag gagatcctga agtctcagct ggacagcctg
     2881 ctgggcctgt accacctgct ggactggttt gccgtggatg agtccaacga ggtggacccc
     2941 gagttctctg cccggctgac cggcatcaag ctggagatgg agccttctct gagcttctac
     3001 aacaaggcca gaaattatgc caccaagaag ccctactccg tggagaagtt caagctgaac
     3061 tttcagatgc ctacactggc ctctggctgg gacgtgaata aggagaagaa caatggcgcc
     3121 atcctgtttg tgaagaacgg cctgtactat ctgggcatca tgccaaagca gaagggcagg
     3181 tataaggccc tgagcttcga gcccacagag aaaaccagcg agggctttga taagatgtac
     3241 tatgactact tccctgatgc cgccaagatg atcccaaagt gcagcaccca gctgaaggcc
     3301 gtgacagccc actttcagac ccacacaacc cccatcctgc tgtccaacaa tttcatcgag
     3361 cctctggaga tcacaaagga gatctacgac ctgaacaatc ctgagaagga gccaaagaag
     3421 tttcagacag cctacgccaa gaaaaccggc gaccagaagg gctacagaga ggccctgtgc
     3481 aagtggatcg acttcacaag ggattttctg tccaagtata ccaagacaac ctctatcgat
     3541 ctgtctagcc tgcggccatc ctctcagtat aaggacctgg gcgagtacta tgccgagctg
     3601 aatcccctgc tgtaccacat cagcttccag agaatcgccg agaaggagat catggatgcc
     3661 gtggagacag gcaagctgta cctgttccag atctataaca aggactttgc caagggccac
     3721 cacggcaagc ctaatctgca cacactgtat tggaccggcc tgttttctcc agagaacctg
     3781 gccaagacaa gcatcaagct gaatggccag gccgagctgt tctaccgccc taagtccagg
     3841 atgaagagga tggcacaccg gctgggagag aagatgctga acaagaagct gaaggatcag
     3901 aaaaccccaa tccccgacac cctgtaccag gagctgtacg actatgtgaa tcacagactg
     3961 tcccacgacc tgtctgatga ggccagggcc ctgctgccca acgtgatcac caaggaggtg
     4021 tctcacgaga tcatcaagga taggcgcttt accagcgaca agttcttttt ccacgtgcct
     4081 atcacactga actatcaggc cgccaattcc ccatctaagt tcaaccagag ggtgaatgcc
     4141 tacctgaagg agcaccccga gacacctatc atcggcatcg atcggggcga gagaaacctg
     4201 atctatatca cagtgatcga ctccaccggc aagatcctgg agcagcggag cctgaacacc
     4261 atccagcagt ttgattacca gaagaagctg gacaacaggg agaaggagag ggtggcagca
     4321 aggcaggcct ggtctgtggt gggcacaatc aaggatctga agcagggcta tctgagccag
     4381 gtcatccacg agatcgtgga cctgatgatc cactaccagg ccgtggtggt gctggcgaac
     4441 ctgaatttcg gctttaagag caagaggacc ggcatcgccg agaaggccgt gtaccagcag
     4501 ttcgagaaga tgctgatcga taagctgaat tgcctggtgc tgaaggacta tccagcagag
     4561 aaagtgggag gcgtgctgaa cccataccag ctgacagacc agttcacctc ctttgccaag
     4621 atgggcaccc agtctggctt cctgttttac gtgcctgccc catatacatc taagatcgat
     4681 cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca tcaagaatca cgagagccgc
     4741 aagcacttcc tggagggctt cgactttctg cactacgacg tgaaaaccgg cgacttcatc
     4801 ctgcacttta agatgaacag aaatctgtcc ttccagaggg gcctgcccgg ctttatgcct
     4861 gcatgggata tcgtgttcga gaagaacgag acacagtttg acgccaaggg cacccctttc
     4921 atcgccggca agagaatcgt gccagtgatc gagaatcaca gattcaccgg cagataccgg
     4981 gacctgtatc ctgccaacga gctgatcgcc ctgctggagg agaagggcat cgtgttcagg
     5041 gatggctcca acatcctgcc aaagctgctg gagaatgacg attctcacgc catcgacacc
     5101 atggtggccc tgatccgcag cgtgctgcag atgcggaact ccaatgccgc cacaggcgag
     5161 gactatatca acagccccgt gcgcgatctg aatggcgtgt gcttcgactc ccggtttcag
     5221 aacccagagt ggcccatgga cgccgatgcc aatggcgcct accacatcgc cctgaagggc
     5281 cagctgctgc tgaatcacct gaaggagagc aaggatctga agctgcagaa cggcatctcc
     5341 aatcaggact ggctggccta catccaggag ctgcgcaaca gcagggctga cgggccctca
     5401 ctgggttcag ggtcacccaa gaagaaacgc aaagtcgagg atccaaagaa gaaaaggaag
     5461 gttgaagacc ccaagaaaaa gaggaaggtg gatgggatcg gctcaggcag caacggcggt
     5521 ggaggttcag acgctttgga cgatttcgat ctcgatatgc tcggttctga cgccctggat
     5581 gatttcgatc tggatatgct cggcagcgac gctctcgacg atttcgacct cgacatgctc
     5641 gggtcagatg ccttggatga ttttgacctg gatatgctct caggatccta cccatacgat
     5701 gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc
     5761 gactatgcct aagaattcga ttcgtcagta gggttgtaaa ggtttttctt ttcctgagaa
     5821 aacaaccttt tgttttctca ggttttgctt tttggccttt ccctagcttt aaaaaaaaaa
     5881 aagcaaaact caccgaggca gttccatagg atggcaagat cctggtattg gtctgcgaaa
     5941 tttctactct tgtagattga agagccagga aacagctatg acgctcttct aatttctact
     6001 cttgtagatt ctagaaaaga attcgatatc aagcttatcg ataatcaacc tctggattac
     6061 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6121 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6181 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6241 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6301 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6361 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6421 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg
     6481 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6541 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6601 agtcggatct ccctttgggc cgcctccccg catcgatacc gagcgctaat aaaagatctt
     6661 tattttcatt agatctgtgt gttggttttt tgtgtcacgt gcggaccgag cggccgcagg
     6721 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     6781 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     6841 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     6901 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     6961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     7021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7141 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7261 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     7321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     7381 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     7441 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7501 ttacagacaa gctgtgacct tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7561 accgaaacgc gcgatacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7621 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9421 gttcctggcc ttttgctggc ct
//
