LOCUS       sequence-401604-        9940 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization 
            signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1
            triplex, dCas12a direct repeats (DR), and a crRNA cloning site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9940)
  TITLE     SGRN: A Cas12a-driven Synthetic Gene Regulatory Network System
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 
            pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA
FEATURES             Location/Qualifiers
     source          1..9940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     520..539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   681..810
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        913..2091
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1144..2082
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2039..2059
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2126..6046
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLANLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5766..5775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6083..6103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6110..6130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6137..6157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6476..6847
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             6854..6934
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     7131..7147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7131..7147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    7208..7796
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7261..7281)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7679..7690)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   7883..8023
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7883..8012
                     /label=AAV2 ITR
     rep_origin      8098..8553
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8185..8204)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8395..8416
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8570..8589)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8689..8711
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8749..8767)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8835..8939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8940..9008,9009..9800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9158..9177)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
       61 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
      181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      661 ctggcctttt gctcacatgt cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc
      721 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
      781 gagtggccaa ctccatcact aggggttcct gcggccgcac gcgtttaatt aatgcaaaga
      841 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
      901 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
      961 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     1021 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     1081 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     1141 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     1201 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     1261 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     1321 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     1381 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     1441 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     1501 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     1561 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     1621 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     1681 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     1741 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     1801 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     1861 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     1921 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     1981 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     2041 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg agctagcgtt
     2101 taaacttaag cttggtaccg ccaccatgac acagttcgag ggctttacca acctgtatca
     2161 ggtgagcaag acactgcggt ttgagctgat cccacagggc aagaccctga agcacatcca
     2221 ggagcagggc ttcatcgagg aggacaaggc ccgcaatgat cactacaagg agctgaagcc
     2281 catcatcgat cggatctaca agacctatgc cgaccagtgc ctgcagctgg tgcagctgga
     2341 ttgggagaac ctgagcgccg ccatcgactc ctatagaaag gagaaaaccg aggagacaag
     2401 gaacgccctg atcgaggagc aggccacata tcgcaatgcc atccacgact acttcatcgg
     2461 ccggacagac aacctgaccg atgccatcaa taagagacac gccgagatct acaagggcct
     2521 gttcaaggcc gagctgttta atggcaaggt gctgaagcag ctgggcaccg tgaccacaac
     2581 cgagcacgag aacgccctgc tgcggagctt cgacaagttt acaacctact tctccggctt
     2641 ttatgagaac aggaagaacg tgttcagcgc cgaggatatc agcacagcca tcccacaccg
     2701 catcgtgcag gacaacttcc ccaagtttaa ggagaattgt cacatcttca cacgcctgat
     2761 caccgccgtg cccagcctgc gggagcactt tgagaacgtg aagaaggcca tcggcatctt
     2821 cgtgagcacc tccatcgagg aggtgttttc cttccctttt tataaccagc tgctgacaca
     2881 gacccagatc gacctgtata accagctgct gggaggaatc tctcgggagg caggcaccga
     2941 gaagatcaag ggcctgaacg aggtgctgaa tctggccatc cagaagaatg atgagacagc
     3001 ccacatcatc gcctccctgc cacacagatt catccccctg tttaagcaga tcctgtccga
     3061 taggaacacc ctgtctttca tcctggagga gtttaagagc gacgaggaag tgatccagtc
     3121 cttctgcaag tacaagacac tgctgagaaa cgagaacgtg ctggagacag ccgaggccct
     3181 gtttaacgag ctgaacagca tcgacctgac acacatcttc atcagccaca agaagctgga
     3241 gacaatcagc agcgccctgt gcgaccactg ggatacactg aggaatgccc tgtatgagcg
     3301 gagaatctcc gagctgacag gcaagatcac caagtctgcc aaggagaagg tgcagcgcag
     3361 cctgaagcac gaggatatca acctgcagga gatcatctct gccgcaggca aggagctgag
     3421 cgaggccttc aagcagaaaa ccagcgagat cctgtcccac gcacacgccg ccctggatca
     3481 gccactgcct acaaccctga agaagcagga ggagaaggag atcctgaagt ctcagctgga
     3541 cagcctgctg ggcctgtacc acctgctgga ctggtttgcc gtggatgagt ccaacgaggt
     3601 ggaccccgag ttctctgccc ggctgaccgg catcaagctg gagatggagc cttctctgag
     3661 cttctacaac aaggccagaa attatgccac caagaagccc tactccgtgg agaagttcaa
     3721 gctgaacttt cagatgccta cactggcctc tggctgggac gtgaataagg agaagaacaa
     3781 tggcgccatc ctgtttgtga agaacggcct gtactatctg ggcatcatgc caaagcagaa
     3841 gggcaggtat aaggccctga gcttcgagcc cacagagaaa accagcgagg gctttgataa
     3901 gatgtactat gactacttcc ctgatgccgc caagatgatc ccaaagtgca gcacccagct
     3961 gaaggccgtg acagcccact ttcagaccca cacaaccccc atcctgctgt ccaacaattt
     4021 catcgagcct ctggagatca caaaggagat ctacgacctg aacaatcctg agaaggagcc
     4081 aaagaagttt cagacagcct acgccaagaa aaccggcgac cagaagggct acagagaggc
     4141 cctgtgcaag tggatcgact tcacaaggga ttttctgtcc aagtatacca agacaacctc
     4201 tatcgatctg tctagcctgc ggccatcctc tcagtataag gacctgggcg agtactatgc
     4261 cgagctgaat cccctgctgt accacatcag cttccagaga atcgccgaga aggagatcat
     4321 ggatgccgtg gagacaggca agctgtacct gttccagatc tataacaagg actttgccaa
     4381 gggccaccac ggcaagccta atctgcacac actgtattgg accggcctgt tttctccaga
     4441 gaacctggcc aagacaagca tcaagctgaa tggccaggcc gagctgttct accgccctaa
     4501 gtccaggatg aagaggatgg cacaccggct gggagagaag atgctgaaca agaagctgaa
     4561 ggatcagaaa accccaatcc ccgacaccct gtaccaggag ctgtacgact atgtgaatca
     4621 cagactgtcc cacgacctgt ctgatgaggc cagggccctg ctgcccaacg tgatcaccaa
     4681 ggaggtgtct cacgagatca tcaaggatag gcgctttacc agcgacaagt tctttttcca
     4741 cgtgcctatc acactgaact atcaggccgc caattcccca tctaagttca accagagggt
     4801 gaatgcctac ctgaaggagc accccgagac acctatcatc ggcatcgatc ggggcgagag
     4861 aaacctgatc tatatcacag tgatcgactc caccggcaag atcctggagc agcggagcct
     4921 gaacaccatc cagcagtttg attaccagaa gaagctggac aacagggaga aggagagggt
     4981 ggcagcaagg caggcctggt ctgtggtggg cacaatcaag gatctgaagc agggctatct
     5041 gagccaggtc atccacgaga tcgtggacct gatgatccac taccaggccg tggtggtgct
     5101 ggcgaacctg aatttcggct ttaagagcaa gaggaccggc atcgccgaga aggccgtgta
     5161 ccagcagttc gagaagatgc tgatcgataa gctgaattgc ctggtgctga aggactatcc
     5221 agcagagaaa gtgggaggcg tgctgaaccc ataccagctg acagaccagt tcacctcctt
     5281 tgccaagatg ggcacccagt ctggcttcct gttttacgtg cctgccccat atacatctaa
     5341 gatcgatccc ctgaccggct tcgtggaccc cttcgtgtgg aaaaccatca agaatcacga
     5401 gagccgcaag cacttcctgg agggcttcga ctttctgcac tacgacgtga aaaccggcga
     5461 cttcatcctg cactttaaga tgaacagaaa tctgtccttc cagaggggcc tgcccggctt
     5521 tatgcctgca tgggatatcg tgttcgagaa gaacgagaca cagtttgacg ccaagggcac
     5581 ccctttcatc gccggcaaga gaatcgtgcc agtgatcgag aatcacagat tcaccggcag
     5641 ataccgggac ctgtatcctg ccaacgagct gatcgccctg ctggaggaga agggcatcgt
     5701 gttcagggat ggctccaaca tcctgccaaa gctgctggag aatgacgatt ctcacgccat
     5761 cgacaccatg gtggccctga tccgcagcgt gctgcagatg cggaactcca atgccgccac
     5821 aggcgaggac tatatcaaca gccccgtgcg cgatctgaat ggcgtgtgct tcgactcccg
     5881 gtttcagaac ccagagtggc ccatggacgc cgatgccaat ggcgcctacc acatcgccct
     5941 gaagggccag ctgctgctga atcacctgaa ggagagcaag gatctgaagc tgcagaacgg
     6001 catctccaat caggactggc tggcctacat ccaggagctg cgcaacagca gggctgacgg
     6061 gccctcactg ggttcagggt cacccaagaa gaaacgcaaa gtcgaggatc caaagaagaa
     6121 aaggaaggtt gaagacccca agaaaaagag gaaggtggga tccggtgggg gtgggtccag
     6181 cgggttgcct aatgggttgg atggtgacga ggattttagc gatatcgccg atatggactt
     6241 ttcagctctc ctcagccaga taagctctgg aagtagtggg cttccaaatg ggcttgacgg
     6301 tgacgaagat ttctctgata tagcggacat ggacttctca gctctgctct cccagatttc
     6361 ttcagggtcc agcggcttgc ccaacggcct cgatggcgac gaggattttt ctgacatagc
     6421 agacatggac ttttccgcac tcctcagtca gatttcttct ggaggcggtg gaagcggctt
     6481 cagcgtggac accagtgccc tgctggacct gttcagcccc tcggtgaccg tgcccgacat
     6541 gagcctgcct gaccttgaca gcagcctggc cagtatccaa gagctcctgt ctccccagga
     6601 gccccccagg cctcccgagg cagagaacag cagcccggat tcagggaagc agctggtgca
     6661 ctacacagcg cagccgctgt tcctgctgga ccccggctcc gtggacaccg ggagcaacga
     6721 cctgccggtg ctgtttgagc tgggagaggg ctcctacttc tccgaagggg acggcttcgc
     6781 cgaggacccc accatctccc tgctgacagg ctcggagcct cccaaagcca aggaccccac
     6841 tgtctccgga tcctacccat acgatgttcc agattacgct tatccctacg acgtgcctga
     6901 ttatgcatac ccatatgatg tccccgacta tgcctaagaa ttcgattcgt cagtagggtt
     6961 gtaaaggttt ttcttttcct gagaaaacaa ccttttgttt tctcaggttt tgctttttgg
     7021 cctttcccta gctttaaaaa aaaaaaagca aaactcaccg aggcagttcc ataggatggc
     7081 aagatcctgg tattggtctg cgaaatttct actcttgtag attgaagagc caggaaacag
     7141 ctatgacgct cttctaattt ctactcttgt agattctaga aaagaattcg atatcaagct
     7201 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7261 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7321 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7381 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7441 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7501 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7561 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7621 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7681 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7741 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     7801 ataccgagcg ctaataaaag atctttattt tcattagatc tgtgtgttgg ttttttgtgt
     7861 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     7921 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     7981 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     8041 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     8101 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     8161 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8221 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8281 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     8341 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8401 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     8461 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8521 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     8581 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     8641 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccttctcc gggagctgca
     8701 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgat acgaaagggc ctcgtgatac
     8761 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     8821 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8881 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8941 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9001 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9061 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9121 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9181 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9241 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9301 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9361 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9421 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9481 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9541 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9601 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9661 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9721 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9781 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9841 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9901 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
//
