LOCUS       sequence-401869-        9124 bp    DNA     circular SYN 15-APR-2025
DEFINITION  a mammalian expression vector to express MAPK14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9124)
  TITLE     bioRxiv- A drug's most potent target is not necessarily the source 
            of its anti-cancer activity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lenti-MAPK14
FEATURES             Location/Qualifiers
     source          1..9124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    94..211
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        260..770
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     689..707
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             792..1391
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(792..811)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1288..1308
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1519..1752
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1824..1945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1861..1880)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1915..1934
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1985..2120
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2140..2159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2141..2159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2169..2186)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2169..2185)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2178..2200)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2327..2782
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2414..2433)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2624..2645
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2808..2912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2913..2981,2982..3773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3131..3150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3944..4532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4433..4452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4900..4921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4936..4941,4942..4959,4960..4966)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4974..4990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4979..5001
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4998..5014
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4998..5014
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5033..5053
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5035..5053
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5081..5307
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5308..5488
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5535..5660
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6153..6386
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6571..6615
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6913..7161
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6913..6933
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7084..7103
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        7316..7695
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7696..7899
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7850..7870
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7896..7920
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7978..8002
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             9011..9019
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(join(9119..9124,1..19))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctttcttgta caaagtggtt gatcgcgtgc atgcgacgtc atagctctct cccaattctc
       61 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
      121 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
      181 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
      241 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
      301 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
      361 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
      421 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
      481 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
      541 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
      601 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
      661 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
      721 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac
      781 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
      841 ccagggcggt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
      901 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
      961 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     1021 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     1081 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     1141 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     1201 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     1261 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     1321 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     1381 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     1441 atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     1501 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     1561 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     1621 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     1681 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     1741 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     1801 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     1861 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1921 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     1981 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2041 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     2101 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     2161 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     2221 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     2281 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     2341 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2401 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     2461 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     2521 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     2581 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2641 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     2701 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     2761 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2821 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2881 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2941 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3001 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3061 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3121 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3181 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3241 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3301 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3361 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3421 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3481 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3541 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3601 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3661 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3721 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3781 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3841 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3901 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3961 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4021 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4081 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4141 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4201 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4261 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4321 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4381 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4441 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4501 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4561 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4621 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4681 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaag ctgataccgc
     4741 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4801 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     4861 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     4921 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     4981 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     5041 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     5101 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     5161 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     5221 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     5281 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     5341 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5401 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5461 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     5521 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     5581 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     5641 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     5701 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     5761 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     5821 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     5881 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     5941 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     6001 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     6061 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     6121 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     6181 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     6241 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     6301 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     6361 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     6421 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     6481 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     6541 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     6601 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     6661 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     6721 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     6781 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     6841 tccattcgat tagtgaacgg atctcgacgg tatcgatcac gagactagcc tcgagcggcc
     6901 gcccccttca ccgagggcct atttcccatg attccttcat atttgcatat acgatacaag
     6961 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     7021 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     7081 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     7141 ttgtggaaag gacgaaacac cggtatctat acattgaatc aatattggca attagccata
     7201 ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
     7261 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat
     7321 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     7381 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     7441 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     7501 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     7561 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     7621 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
     7681 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     7741 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     7801 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     7861 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     7921 gcctggagac gccatccacg ctgttttgac ctccatagaa gattctagat tcgaagaaca
     7981 agtttgtaca aaaaagcagg cttggaagga gttcgaacca tgtctcagga gaggcccacg
     8041 ttctaccggc aggagctgaa caagacaatc tgggaggtgc ccgagcgtta ccagaacctg
     8101 tctccagtgg gctctggcgc ctatggctct gtgtgtgctg cttttgacac gaaaacgggg
     8161 ttacgtgtgg cagtgaagaa gctctccaga ccatttcagt ccatcattca tgcgaaaaga
     8221 acctacagag aactgcggtt acttaaacac atgaaacatg aaaatgtgat tggtctgttg
     8281 gacgttttta cacctgcaag gtctctggag gaattcaatg atgtgtatct ggtgacccat
     8341 ctcatggggg cagatctgaa caacattgtg aaatgtcaga agcttacaga tgaccatgtt
     8401 cagttcctta tctaccaaat tctccgaggt ctaaagtata tacattcagc tgacataatt
     8461 cacagggacc taaaacctag taatctagct gtgaatgaag actgtgagct gaagattctg
     8521 gattttggac tggctcggca cacagatgat gaaatgacag gctacgtggc cactaggtgg
     8581 tacagggctc ctgagatcat gctgaactgg atgcattaca accagacagt tgatatttgg
     8641 tcagtgggat gcataatggc cgagctgttg actggaagaa cattgtttcc tggtacagac
     8701 catattaacc agcttcagca gattatgcgt ctgacaggaa caccccccgc ttatctcatt
     8761 aacaggatgc caagccatga ggcaagaaac tatattcagt ctttgactca gatgccgaag
     8821 atgaactttg cgaatgtatt tattggtgcc aatcccctgg ctgtcgactt gctggagaag
     8881 atgcttgtat tggactcaga taagagaatt acagcggccc aagcccttgc acatgcctac
     8941 tttgctcagt accacgatcc tgatgatgaa ccagtggccg atccttatga tcagtccttt
     9001 gaaagcaggg acctccttat agatgagtgg aaaagcctga cctatgatga agtcatcagc
     9061 tttgtgccac caccccttga ccaagaagag atggagtcct agctcgagtg cggccgcaac
     9121 ccag
//
