LOCUS       sequence-402561-        9652 bp    DNA     circular SYN 15-APR-2025
DEFINITION  RepCap for AAV production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Huang Q, Chan KY, Wu J, Botticello-Romero NR, Zheng Q, Lou S, Keyes 
            C, Svanbergsson A, Johnston J, Mills A, Lin CY, Brauer PP, Clouse G,
            Pacouret S, Harvey JW, Beddow T, Hurley JK, Tobey IG, Powell M, Chen
            AT, Barry AJ, Eid FE, Chan YA, Deverman BE
  TITLE     An AAV capsid reprogrammed to bind human transferrin receptor 
            mediates brain-wide gene delivery.
  JOURNAL   Science. 2024 Jun 14;384(6701):1220-1227. doi: 
            10.1126/science.adm8386. Epub 2024 May 16.
  PUBMED    38753766
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adm8386"; journalName: "Science"; date: 
            "2024-06-14- 14"; volume: "384"; issue: "6701"; pages: "1220-1227"
COMMENT     Sequence Label: iCAP-BI103-KanR
FEATURES             Location/Qualifiers
     source          1..9652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..73
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        83..101
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     131..147
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2711..2821
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2860..2878)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2861..2878)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2898..2915)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2904..3128
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3190..3208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3227..3245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3264..3282
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3301..3319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3338..3356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3375..3393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3412..3430
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3449..3467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6184..6195
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6647..6667)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6647..6665)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6686..6702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6686..6702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6699..6721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6710..6726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6734..6740,6741..6758,6759..6764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6779..6800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6917..6934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7088..7676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7168..7187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7850..8659)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8573..8592
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8660..8764)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8790..9245)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8927..8948)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9139..9158
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9387..9403
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9413..9431
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9461..9477
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgggctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
       61 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      121 ccccctcgat cgaggtcgac ggtatcgggg gagctcgcag ggtctccatt ttgaagcggg
      181 aggtttgaac gcgcagccgc catgccgggg ttttacgaga ttgtgattaa ggtccccagc
      241 gaccttgacg agcatctgcc cggcatttct gacagctttg tgaactgggt ggccgagaag
      301 gaatgggagt tgccgccaga ttctgacatg gatctgaatc tgattgagca ggcacccctg
      361 accgtggccg agaagctgca gcgcgacttt ctgacggaat ggcgccgtgt gagtaaggcc
      421 ccggaggctc ttttctttgt gcaatttgag aagggagaga gctacttcca catgcacgtg
      481 ctcgtggaaa ccaccggggt gaaatccatg gttttgggac gtttcctgag tcagattcgc
      541 gaaaaactga ttcagagaat ttaccgcggg atcgagccga ctttgccaaa ctggttcgcg
      601 gtcacaaaga ccagaaatgg cgccggaggc gggaacaagg tggtggatga gtgctacatc
      661 cccaattact tgctccccaa aacccagcct gagctccagt gggcgtggac taatatggaa
      721 cagtatttaa gcgcctgttt gaatctcacg gagcgtaaac ggttggtggc gcagcatctg
      781 acgcacgtgt cgcagacgca ggagcagaac aaagagaatc agaatcccaa ttctgatgcg
      841 ccggtgatca gatcaaaaac ttcagccagg tacatggagc tggtcgggtg gctcgtggac
      901 aaggggatta cctcggagaa gcagtggatc caggaggacc aggcctcata catctccttc
      961 aatgcggcct ccaactcgcg gtcccaaatc aaggctgcct tggacaatgc gggaaagatt
     1021 atgagcctga ctaaaaccgc ccccgactac ctggtgggcc agcagcccgt ggaggacatt
     1081 tccagcaatc ggatttataa aattttggaa ctaaacgggt acgatcccca atatgcggct
     1141 tccgtctttc tgggatgggc cacgaaaaag ttcggcaaga ggaacaccat ctggctgttt
     1201 gggcctgcaa ctaccgggaa gaccaacatc gcggaggcca tagcccacac tgtgcccttc
     1261 tacgggtgcg taaactggac caatgagaac tttcccttca acgactgtgt cgacaagatg
     1321 gtgatctggt gggaggaggg gaagatgacc gccaaggtcg tggagtcggc caaagccatt
     1381 ctcggaggaa gcaaggtgcg cgtggaccag aaatgcaagt cctcggccca gatagacccg
     1441 actcccgtga tcgtcacctc caacaccaac atgtgcgccg tgattgacgg gaactcaacg
     1501 accttcgaac accagcagcc gttgcaagac cggatgttca aatttgaact cacccgccgt
     1561 ctggatcatg actttgggaa ggtcaccaag caggaagtca aagacttttt ccggtgggca
     1621 aaggatcacg tggttgaggt ggagcatgaa ttctacgtca aaaagggtgg agccaagaaa
     1681 agacccgccc ccagtgacgc agatataagt gagcccaaac gggtgcgcga gtcagttgcg
     1741 cagccatcga cgtcagacgc ggaagcttcg atcaactacg cggacaggta ccaaaacaaa
     1801 tgttctcgtc acgtgggcat gaatctgatg ctgtttccct gcagacaatg cgagagactg
     1861 aatcagaatt caaatatctg cttcactcac ggtgtcaaag actgtttaga gtgctttccc
     1921 gtgtcagaat ctcaacccgt ttctgtcgtc aaaaaggcgt atcagaaact gtgctacatt
     1981 catcacatca tgggaaaggt gccagacgct tgcactgctt gcgacctggt caatgtggac
     2041 ttggatgact gtgtttctga acaataaatg acttaaacca ggtatgagtc ggctggataa
     2101 atctaaagtc ataaacggcg ctctggaatt actcaatgaa gtcggtatcg aaggcctgac
     2161 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     2221 gaacaagcgg gccctgctcg atgccctggc catcgagatg ctggacaggc atcataccca
     2281 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt
     2341 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     2401 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     2461 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     2521 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     2581 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     2641 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     2701 cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     2761 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     2821 ccccgggtaa atgcatgaat tcgatctaga gggccctatt ctatagtgtc acctaaatgc
     2881 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2941 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3001 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3061 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     3121 ctctatggct tctgaggcgg aaagaaccag ctggggctcg aatcaagcta tcaagtgcca
     3181 cctgacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat
     3241 agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc
     3301 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc
     3361 gacacgtctc gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca
     3421 gtgatagaga agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat
     3481 ataagcagag agatctgttc aaatttgaac tgactaagcg gctcccgcca gattttggca
     3541 agattactaa gcaggaagtc aaggactttt ttgcttgggc aaaggtcaat caggtgccgg
     3601 tgactcacga gtttaaagtt cccagggaat tggcgggaac taaaggggcg gagaaatctc
     3661 taaaacgccc actgggtgac gtcaccaata ctagctataa aagtctggag aagcgggcca
     3721 ggctctcatt tgttcccgag acgcctcgca gttcagacgt gactgttgat cccgctcctc
     3781 tgcgaccgct agcttcgatc aactacgcgg acaggtacca aaacaaatgt tctcgtcacg
     3841 tgggcatgaa tctgatgctg tttccctgca gacaatgcga gagactgaat cagaattcaa
     3901 atatctgctt cactcacggt gtcaaagact gtttagagtg ctttcccgtg tcagaatctc
     3961 aacccgtttc tgtcgtcaaa aaggcgtatc agaaactgtg ctacattcat cacatcatgg
     4021 gaaaggtgcc agacgcttgc actgcttgcg acctggtcaa tgtggacttg gatgactgtg
     4081 tttctgaaca ataaatgact taaaccaggt atggctgccg atggttatct tccagattgg
     4141 ctcgaggaca accttagtga aggaattcgc gagtggtggg ctttgaaacc tggagcccct
     4201 caacccaagg caaatcaaca acatcaagac aacgctcgag gtcttgtgct tccgggttac
     4261 aaataccttg gacccggcaa cggactcgac aagggggagc cggtcaacgc agcagacgcg
     4321 gcggccctcg agcacgacaa ggcctacgac cagcagctca aggccggaga caacccgtac
     4381 ctcaagtaca accacgccga cgccgagttc caggagcggc tcaaagaaga tacgtctttt
     4441 gggggcaacc tcgggcgagc agtcttccag gccaaaaaga ggcttcttga acctcttggt
     4501 ctggttgagg aagcggctaa gacggctcct ggaaagaaga ggcctgtaga gcagtctcct
     4561 caggaaccgg actcctccgc gggtattggc aaatcgggtg cacagcccgc taaaaagaga
     4621 ctcaatttcg gtcagactgg cgacacagag tcagtcccag accctcaacc aatcggagaa
     4681 cctcccgcag ccccctcagg tgtgggatct cttacaatgg cttcaggtgg tggcgcacca
     4741 gtggcagaca ataacgaagg tgccgatgga gtgggtagtt cctcgggaaa ttggcattgc
     4801 gattcccaat ggctggggga cagagtcatc accaccagca cccgaacctg ggccctgccc
     4861 acctacaaca atcacctcta caagcaaatc tccaacagca catctggagg atcttcaaat
     4921 gacaacgcct acttcggcta cagcaccccc tgggggtatt ttgacttcaa cagattccac
     4981 tgccacttct caccacgtga ctggcagcga ctcatcaaca acaactgggg attccggcct
     5041 aagcgactca acttcaagct cttcaacatt caggtcaaag aggttacgga caacaatgga
     5101 gtcaagacca tcgccaataa ccttaccagc acggtccagg tcttcacgga ctcagactat
     5161 cagctcccgt acgtgctcgg gtcggctcac gagggctgcc tcccgccgtt cccagcggac
     5221 gttttcatga ttcctcagta cgggtatctg acgcttaatg atggaagcca ggccgtgggt
     5281 cgttcgtcct tttactgcct ggaatatttc ccgtcgcaaa tgctaagaac gggtaacaac
     5341 ttccagttca gctacgagtt tgagaacgta cctttccata gcagctacgc tcacagccaa
     5401 agcctggacc gactaatgaa tccactcatc gaccaatact tgtactatct ctctagaact
     5461 attaacggtt ctggacagaa tcaacaaacg ctaaaattca gtgtggccgg acccagcaac
     5521 atggctgtcc agggaagaaa ctacatacct ggacccagct accgacaaca acgtgtctca
     5581 accactgtga ctcaaaacaa caacagcgaa tttgcttggc ctggagcttc ttcttgggct
     5641 ctcaatggac gtaatagctt gatgaatcct ggacctgcta tggccagcca caaagaagga
     5701 gaggaccgtt tctttccttt gtctggatct ttaatttttg gcaaacaagg taccggcaga
     5761 gacaacgtgg atgcggacaa agtcatgata accaacgaag aagaaattaa aactactaac
     5821 ccggtagcaa cggagtccta tggacaagtg gccacaaacc accagagtgc acaaatcagg
     5881 gtacccaatg aatttgcaca ggctcaaacc ggttgggttc aaaaccaagg aatacttccg
     5941 ggtatggttt ggcaggacag agatgtgtac ctgcaaggac ccatttgggc caaaattcct
     6001 cacacggacg gcaactttca cccttctccg ctgatgggag ggtttggaat gaagcacccg
     6061 cctcctcaga tcctcatcaa aaacacacct gtacctgcgg atcctccaac ggccttcaac
     6121 aaggacaagc tgaactcttt catcacccag tattctactg gccaagtcag cgtggagatc
     6181 gagtgggagc tgcagaagga aaacagcaag cgctggaacc cggagatcca gtacacttcc
     6241 aactattaca agtctaataa tgttgaattt gctgttaata ctgaaggtgt atatagtgaa
     6301 ccccgcccca ttggcaccag atacctgact cgtaatctgt aattgcttgt taatcaataa
     6361 accgtttaat tcgtttcagt tgaactttgg tctctgcgaa gggcgaattc gtttaaacct
     6421 gcaggactag aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt ttgcgacacc
     6481 atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat tttgaagcgg
     6541 gaggtttgaa cgcgcagccg ccaagccgaa ttctgcagat atccatcaca ctggcggccg
     6601 ctcgactaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     6661 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6721 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6781 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6841 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6901 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6961 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7081 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7501 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7681 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7741 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7801 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat tagaaaaact
     7861 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     7921 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     7981 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     8041 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     8101 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     8161 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     8221 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     8281 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     8341 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     8401 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     8461 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     8521 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     8581 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgacgttt
     8641 cccgttgaat atggctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8761 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     8821 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     8881 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     8941 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     9001 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     9061 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     9121 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     9181 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     9241 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     9301 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     9361 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     9421 ctcactatag ggcgaattgg gtaccgggcc ccccctcgat cgaggtcgac ggtatcgggg
     9481 gagctcgcag ggtctccatt ttgaagcggg aggtttgaac gcgcagccgc catgccgggg
     9541 ttttacgaga ttgtgattaa ggtccccagc gaccttgacg agcatctgcc ccgcattggg
     9601 gdgttgctyg tgaacagagg agccaagtga ttgagggagg ggggggcaga gg
//
