LOCUS       sequence-402703-        1697 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  in vitro transcription of RNA encoding human ACVR1 with mutations 
            T189V, S190A, and R206H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Allen RS, Jones WD, Hale M, Warder BN, Shore EM, Mullins MC
  TITLE     Reduced GS domain serine/threonine requirements of Fibrodysplasia 
            Ossificans Progressiva mutant type I BMP receptor ACVR1 in the 
            zebrafish.
  JOURNAL   J Bone Miner Res. 2023 Jun 17. doi: 10.1002/jbmr.4869.
  PUBMED    37329499
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Bone 
            Miner Res. 2023 Jun 17. doi: 10.1002/jbmr.4869."
COMMENT     Sequence Label: ACVR1-VASS-R206H
FEATURES             Location/Qualifiers
     source          1..1697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     55..72
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1650..1697
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 agcatgctcg tttagggaac cgccattctg cctgggaacg tcggagcaag cttgatttag
       61 gtgacactat agaatacaag ctacttgttc tttttgcagg atccatggta gatggagtga
      121 tgattcttcc tgtgcttatc atgattgctc tcccctcccc tagtatggaa gatgagaagc
      181 ccaaggtcaa ccccaaactc tacatgtgtg tgtgtgaagg tctctcctgc ggtaatgagg
      241 accactgtga aggccagcag tgcttttcct cactgagcat caacgatggc ttccacgtct
      301 accagaaagg ctgcttccag gtttatgagc agggaaagat gacctgtaag accccgccgt
      361 cccctggcca agctgtggag tgctgccaag gggactggtg taacaggaac atcacggccc
      421 agctgcccac taaaggaaaa tccttccctg gaacacagaa tttccacttg gaggttggcc
      481 tcattattct ctctgtagtg ttcgcagtat gtcttttagc ctgcctgctg ggagttgctc
      541 tccgaaaatt taaarggcgc aaccaagaac gcctcaatcc ccgagacgtg gagtatggca
      601 ctatcgaagg gctcatcacc accaatgttg gagacagcac tttagcagat ttattggatc
      661 attcgtgtgt agcaggaagt ggctctggtc ttccttttct ggtacaaaga acagtggctc
      721 accagattac actgttggag tgtgtcggga aaggcaggta tggtgaggtg tggaggggca
      781 gctggcaagg ggaaaatgtt gccgtgaaga tcttctcctc ccgtgatgag aagtcatggt
      841 tcagggaaac ggaattgtac aacactgtga tgctgaggca tgaaaatatc ttaggtttca
      901 ttgcttcaga catgacatca agacactcca gtacccagct gtggttaatt acacattatc
      961 atgaaatggg atcgttgtac gactatcttc agcttactac tctggataca gttagctgcc
     1021 ttcgaatagt gctgtccata gctagtggtc ttgcacattt gcacatagag atatttggga
     1081 cccaagggaa accagccatt gcccatcgag atttaaagag caaaaatatt ctggttaaga
     1141 agaatggaca gtgttgcata gcagatttgg gcctggcagt catgcattcc cagagcacca
     1201 atcagcttga tgtggggaac aatccccgtg tgggcaccaa gcgctacatg gcccccgaag
     1261 ttctagatga aaccatccag gtggattgtt tcgattctta taaaagggtc gatatttggg
     1321 cctttggact tgttttgtgg gaagtggcca ggcggatggt gagcaatggt atagtggagg
     1381 attacaagcc accgttctac gatgtggttc ccaatgaccc aagttttgaa gatatgagga
     1441 aggtagtctg tgtggatcaa caaaggccaa acatacccaa cagatggttc tcagacccga
     1501 cattaacctc tctggccaag ctaatgaaag aatgctggta tcaaaatcca tccgcaagac
     1561 tcacagcact gcgtatcaaa aagactttga ccaaaattga taattccctc gacaaattga
     1621 aaactgactg taatcgattt aaagctatgg attacaagga tgatgatgat aaggattaca
     1681 aggatgatga tgataag
//
