LOCUS       sequence-402861-        8995 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Centromeric yeast expression vector, leucine selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Galosi S, Edani BH, Martinelli S, Hansikova H, Eklund EA, Caputi C, 
            Masuelli L, Corsten-Janssen N, Srour M, Oegema R, Bosch DGM, Ellis 
            CA, Amlie-Wolf L, Accogli A, Atallah I, Averdunk L, Baranano KW, Bei
            R, Bagnasco I, Brusco A, Demarest S, Alaix AS, Di Bonaventura C, 
            Distelmaier F, Elmslie F, Gan-Or Z, Good JM, Gripp K, Kamsteeg EJ, 
            Macnamara E, Marcelis C, Mercier N, Peeden J, Pizzi S, Pannone L, 
            Shinawi M, Toro C, Verbeek NE, Venkateswaran S, Wheeler PG, 
            Zdrazilova L, Zhang R, Zorzi G, Guerrini R, Sessa WC, Lefeber DJ, 
            Tartaglia M, Hamdan FF, Grabinska KA, Leuzzi V
  TITLE     De novo DHDDS variants cause a neurodevelopmental and 
            neurodegenerative disorder with myoclonus.
  JOURNAL   Brain. 2022 Mar 29;145(1):208-223. doi: 10.1093/brain/awab299.
  PUBMED    34382076
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Brain"; date: "2022-03-29- 29"; volume: "145"; issue: "1"; pages: 
            "208-223"
COMMENT     Sequence Label: pTL
FEATURES             Location/Qualifiers
     source          1..8995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    34..158
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(183..188,189..206,207..213)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             267..926
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(333..352)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1268..1573
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1452..1471
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1614..1738)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1784..2031
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1800..1818)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(2054..2070)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2054..2070)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2067..2089)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2078..2094
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2102..2108,2109..2126,2127..2132))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2147..2168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2285..2302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2456..3044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2536..2555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3215..4006,4007..4075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3838..3857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4076..4180)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4248..4266
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             4829..5923
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYA
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVRHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    6718..7880
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      6718..7555
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     primer_bind     8622..8644
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8636..8653
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8637..8653
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttcgggggat ccgtttaaac aggcctgtta tcaacaagtt tgtacaaaaa agctgaacga
       61 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      121 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
      181 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
      241 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
      301 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
      361 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
      421 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
      481 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
      541 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
      601 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
      661 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
      721 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
      781 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
      841 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
      901 gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac
      961 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     1021 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
     1081 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     1141 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     1201 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
     1261 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     1321 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     1381 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     1441 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     1501 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     1561 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
     1621 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     1681 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
     1741 ataacatcga tagcggccgc tagggccctg caggagggcc gcatcatgta attagttatg
     1801 tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga
     1861 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
     1921 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
     1981 ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggccaagct
     2041 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2101 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2161 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2221 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2281 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2341 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2401 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2461 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2521 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2581 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2641 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2701 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2761 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2821 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2881 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2941 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3001 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3061 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3121 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3181 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3241 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3301 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3361 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3421 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3481 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3541 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3601 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3661 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3721 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3781 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3841 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3901 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3961 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4021 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4081 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4141 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4201 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4261 cgaggccctt tcgtcttcaa gaattagctt ttcaattcat catttttttt ttattctttt
     4321 ttttgatttc ggtttccttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa
     4381 cgaaggaagg agcacagact tagattggta tatatacgca tatggtgtgg gaatactcag
     4441 gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttc ctcaacataa
     4501 cgagaacaca caggggcgct atcgcacaga atcaaattcg atgactggaa attttttgtt
     4561 aatttcagag gtcgcctgac gcatatacct ttttcaactg aaaaattggg agaaaaagga
     4621 aaggtgagag cgccggaacc ggcttttcat atagaataga gaagcgttca tgactaaatg
     4681 cttgcatcac aatacttgaa gttgacaata ttatttaagg acctattgtt ttttccaata
     4741 ggtggttagc aatcgtctta ctttctaact tttcttacct tttacatttc agcaatatat
     4801 atatatattt caaggatata ccattctaat gtctgcccct aagaagatcg tcgttttgcc
     4861 aggtgaccac gttggtcaag aaatcacagc cgaagccatt aaggttctta aagctatttc
     4921 tgatgttcgt tccaatgtca agttcgattt cgaaaatcat ttaattggtg gtgctgctat
     4981 cgatgctaca ggtgttccac ttccagatga ggcgctggaa gcctccaaga aggctgatgc
     5041 cgttttgtta ggtgctgtgg gtggtcctaa atggggtacc ggtagtgtta gacctgaaca
     5101 aggtttacta aaaatccgta aagaacttca attgtacgcc aacttaagac catgtaactt
     5161 tgcatccgac tctcttttag acttatctcc aatcaagcca caatttgcta aaggtactga
     5221 cttcgttgtt gtcagagaat tagtgggagg tatttacttt ggtaagagaa aggaagacga
     5281 tggtgatggt gtcgcttggg atagtgaaca atacgccgtt ccagaagtgc aaagaatcac
     5341 aagaatggcc gctttcatgg ccctacaaca tgagccacca ttgcctattt ggtccttgga
     5401 taaagctaat gttttggcct cttcaagatt atggagaaaa actgtggagg aaaccatcaa
     5461 gaacgaattc cctacattga aggttcgaca tcaattgatt gattctgccg ccatgatcct
     5521 agttaagaac ccaacccacc taaatggtat tataatcacc agcaacatgt ttggtgatat
     5581 catctccgat gaagcctccg ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt
     5641 ggcctctttg ccagacaaga acaccgcatt tggtttgtac gaaccatgcc acggttctgc
     5701 tccagatttg ccaaagaata aggtcaaccc tatcgccact atcttgtctg ctgcaatgat
     5761 gttgaaattg tcattgaact tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa
     5821 ggttttggat gcaggtatca gaactggtga tttaggtggt tccaacagta ccaccgaagt
     5881 cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt ctcttttttt
     5941 atgatatttg tacataaact ttataaatga aattcataat agaaacgaca cgaaattaca
     6001 aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata catttatcaa
     6061 gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact aatggctcaa
     6121 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca
     6181 caaaaagtta ggtgtaacag aaaatcatga aactatgatt cctaatttat atattggagg
     6241 attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg
     6301 atggagttta agtcaatacc ttcttgaacc atttcccata atggtgaaag ttccctcaag
     6361 aattttactc tgtcagaaac ggccttaacg acgtaccgga tcatgcatgt atactaaact
     6421 cacaaattag agcttcaatt taattatatc agttattacc caattctcat gtttgacagc
     6481 ttatcatcga tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc
     6541 ggtttgccag ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca
     6601 gcttcacaga aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt
     6661 gaacttttgg attggaactc gatttctgac tgggttggaa ggcaagagag ccccgagagc
     6721 ttacatttta tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta
     6781 aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct
     6841 aacaaaatag caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta
     6901 tttaagtatt gtttgtgcac ttgcctgcaa gccttttgaa aagcaagcat aaaagatcta
     6961 aacataaaat ctgtaaaata acaagatgta aagataatgc taaatcattt ggctttttga
     7021 ttgattgtac aggaaaatat acatcgcagg gggttgactt ttaccatttc accgcaatgg
     7081 aatcaaactt gttgaagaga atgttcacag gcgcatacgc tacaatgacc cgattcttgc
     7141 tagccttttc tcggtcttgc aaacaaccgc cggcagctta gtatataaat acacatgtac
     7201 atacctctct ccgtatcctc gtaatcattt tcttgtattt atcgtctttt cgctgtaaaa
     7261 actttatcac acttatctca aatacactta ttaaccgctt ttactattat cttctacgct
     7321 gacagtaata tcaaacagtg acacatatta aacacagtgg tttctttgca taaacaccat
     7381 cagcctcaag tcgtcaagta aagatttcgt gttcatgcag atagataaca atctatatgt
     7441 tgataattag cgttgcctca tcaatgcgag atccgtttaa ccggacccta gtgcacttac
     7501 cccacgttcg gtccactgtg tgccgaacat gctccttcac tattttaaca tgtggaatta
     7561 attctcatgt ttgacagctt atcatcgaac tctaagaggt gatacttatt tactgtaaaa
     7621 ctgtgacgat aaaaccggaa ggaagaataa gaaaactcga actgatctat aatgcctatt
     7681 ttctgtaaag agtttaagct atgaaagcct cggcattttg gccgctccta ggtagtgctt
     7741 tttttccaag gacaaaacag tttctttttc ttgagcaggt tttatgtttc ggtaatcata
     7801 aacaataaat aaattatttc atttatgttt aaaaataaaa aataaaaaag tattttaaat
     7861 ttttaaaaaa gttgattata agcatgtgac cttttgcaag caattaaatt ttgcaatttg
     7921 tgatttaggc aaaagttact atttctggct cgtgtaatat atgtatgcta atgtgaactt
     7981 ttacaaagtc gatatggact tagtcaaaag aaattttctt aaaaatatat agcactagcc
     8041 aatttagcac ttctttatga gatatattat agactttatt aagccagatt tgtgtattat
     8101 atgtatttac ccggcgaatc atggacatac attctgaaat aggtaatatt ctctatggtg
     8161 agacagcata gataacctag gatacaagtt aaaagctagt actgttttgc agtaattttt
     8221 ttctttttta taagaatgtt accacctaaa taagttataa agtcaatagt taagtttgat
     8281 atttgattgt aaaataccgt aatatatttg catgatcaaa aggctcaatg ttgactagcc
     8341 agcatgtcaa ccactatatt gatcaccgat attaggactt ccacaccaac tagtaatatg
     8401 acaataaatt caagatattc ttcatgagaa tggcccagcg attatgcggt gtgaaatacc
     8461 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     8521 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     8581 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     8641 aacgacggcc agtgaattct tattacgatc ctcgcgcccc ctacccaccg tactcgtcaa
     8701 ttccaagggc atcggtaaac atctgctcaa actcgaagtc ggccatatcc agagcgccgt
     8761 agggggcgga gtcgtggggg gtaaatcccg gacccgggga atccccgtcc cccaacatgt
     8821 ccagatcgaa atcgtctagc gcgtcggcat gcatgtgctc tgtatgtata taaaactctt
     8881 gttttcttct tttctctaaa tattctttcc ttatacatta ggtcctttgt agcataaatt
     8941 actatacttc tatagacacg caaacacaaa tacacacact aaattaccgg atcaa
//
