LOCUS       sequence-402964-       11147 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-NR4A3-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..11147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             831..1011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1058..1183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2436..2553
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2610..2628,2629..2651,2652..2670,2671..2693,2694..2712,
                     2713..2735,2736..2754,2755..2777,2778..2796,2797..2819,
                     2820..2838,2839..2861,2862..2880)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2913..2951
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2948..2972
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3373..3402
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             3376..3405
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             3379..3408
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             3382..3411
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             3385..3414
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4993..5049
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5059..5766
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5201..5219)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5469..5488
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5789..6377
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5842..5862)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6260..6271)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6380..6396)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6381..6397)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6948..7128
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7154..7171)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7160..7384
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7430..7858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7517..7536)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7727..7748
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7867..7887)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7872..8201
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8052..8187
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8114..8133
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8249..8296
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8315..8689
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8819..8940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8856..8875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8910..8929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8989..9005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8989..9005)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9002..9024)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9013..9029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9037..9043,9044..9061,9062..9067))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9082..9103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9220..9237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9391..9979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9471..9490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10150..10941,10942..11010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10773..10792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11011..11115)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
      841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1021 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1081 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1141 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1201 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1261 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1321 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1381 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1441 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1501 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     2401 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     2461 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2521 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     2581 ccagtttggt tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtggaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     2941 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     3001 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc cgccaccatg
     3061 catgactcaa tcagatttgg aaatgtggat atgccctgcg tccaagccca atatagccct
     3121 tcccctccag gttccagtta tgcggcgcag acatacagct cggaatacac cacggagatc
     3181 atgaaccccg actacaccaa gctgaccatg gaccttggca gcactgagat cacggctaca
     3241 gccaccacgt ccctgcccag catcagtacc ttcgtggagg gctactcgag caactacgaa
     3301 ctcaagcctt cctgcgtgta ccaaatgcag cggcccttga tcaaagtgga ggaggggcgg
     3361 gcgcccagct accatcacca tcaccaccac caccaccacc accaccacca tcaccagcag
     3421 cagcatcagc agccatccat tcctccagcc tccagcccgg aggacgaggt gctgcccagc
     3481 acctccatgt acttcaagca gtccccaccg tccaccccca ccacgccggc cttccccccg
     3541 caggcggggg cgttatggga cgaggcactg ccctcggcgc ccggctgcat cgcacccggc
     3601 ccgctgctgg acccgccgat gaaggcggtc cccacggtgg ccggcgcgcg cttcccgctc
     3661 ttccacttca agccctcgcc gccgcatccc cccgcgccca gcccggccgg cggccaccac
     3721 ctcggctacg acccgacggc cgctgccgcg ctcagcctgc cgctgggagc cgcagccgcc
     3781 gcgggcagcc aggccgccgc gcttgagggc cacccgtacg ggctgccgct ggccaagagg
     3841 gcggccccgc tggccttccc gcctctcggc ctcacgccct cccctaccgc gtccagcctg
     3901 ctgggcgaga gtcccagcct gccgtcgccg cccagcagga gctcgtcgtc tggcgagggc
     3961 acgtgtgccg tgtgcgggga caacgccgcc tgccagcact acggcgtgcg aacctgcgag
     4021 ggctgcaagg gctttttcaa gagaacagtg cagaaaaatg caaaatatgt ttgcctggca
     4081 aataaaaact gcccagtaga caagagacgt cgaaaccgat gtcagtactg tcgatttcag
     4141 aagtgtctca gtgttggaat ggtaaaagaa gttgtccgta cagatagtct gaaagggagg
     4201 agaggtcgtc tgccttccaa accaaagagc ccattacaac aggaaccttc tcagccctct
     4261 ccaccttctc ctccaatctg catgatgaat gctcttgtcc gagctttaac agactcaaca
     4321 cccagagatc ttgattattc cagatactgt cccactgacc aggctgctgc aggcacagat
     4381 gctgagcatg tgcaacaatt ctacaacctc ctgacagcct ccattgatgt atccagaagc
     4441 tgggcagaaa agattccggg atttactgat ctccccaaag aagatcagac attacttatt
     4501 gaatcagcct ttttggagct gtttgtcctc agactttcca tcaggtcaaa cactgctgaa
     4561 gataagtttg tgttctgcaa tggacttgtc ctgcatcgac ttcagtgcct tcgtggattt
     4621 ggggagtggc tcgactctat taaagacttt tccttaaatt tgcagagcct gaaccttgat
     4681 atccaagcct tagcctgcct gtcagcactg agcatgatca cagaaagaca tgggttaaaa
     4741 gaaccaaaga gagtcgaaga gctatgcaac aagatcacaa gcagtttaaa agaccaccag
     4801 agtaagggac aggctctgga gcccaccgag tccaaggtcc tgggtgccct ggtagaactg
     4861 aggaagatct gcaccctggg cctccagcgc atcttctacc tgaagctgga agacttggtg
     4921 tctccacctt ccatcattga caagctcttc ctggacaccc tacctttcac gcgtgaattc
     4981 gatggatccg gggccaccaa cttttccctg ctgaagcagg ctggcgacgt cgaggagaac
     5041 cctggccctg gctccggagt gagcaagggc gaggaggata acatggccat catcaaggag
     5101 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     5161 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     5221 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     5281 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     5341 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     5401 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     5461 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     5521 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     5581 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     5641 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     5701 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     5761 aagtgaatct agaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     5821 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5881 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5941 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6001 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6061 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6121 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6181 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6241 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6301 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6361 ttgggccgct tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     6421 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     6481 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     6541 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6601 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     6661 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     6721 caaggctaga ggaaacccac aatatcgctt agtcaccttt gctagcagaa ctagtaccag
     6781 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     6841 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     6901 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     6961 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7021 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7081 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     7141 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     7201 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     7261 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     7321 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     7381 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     7441 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7501 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7561 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7621 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7681 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7741 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7801 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     7861 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7921 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7981 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     8041 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8101 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     8161 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     8221 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     8281 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     8341 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     8401 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     8461 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     8521 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     8581 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     8641 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     8701 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     8761 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     8821 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8881 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8941 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     9001 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9061 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9121 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9181 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9241 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9301 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9361 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9421 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9481 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9541 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9601 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9661 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9721 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9781 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9841 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9901 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9961 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10021 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10081 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10141 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10201 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10261 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10321 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10381 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10441 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10501 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10561 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10621 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10681 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10741 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10801 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10861 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10921 ttttaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10981 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11041 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11101 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcg
//
