LOCUS       sequence-403203-       17113 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Fly transformation vector expressing indicated Wnk variant under 
            tubulin promoter for ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17113)
  AUTHORS   Jenny Lab
  TITLE     Jenny Lab Wnk Plasmids 2023
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 17113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pAttB-Tub-HA-WnkDeltaCT
FEATURES             Location/Qualifiers
     source          1..17113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    156..237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(193..212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     247..266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    338..407
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(626..644)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(665..681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(665..681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(678..700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    689..705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(713..719,720..737,738..743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    758..779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(896..913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1067..1655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1147..1166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1826..2617,2618..2686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2449..2468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2687..2791)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2817..3272)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2954..2975)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3166..3185
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3399..3421
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3413..3430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3414..3430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3437..3456
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3437..3455
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3489..3507)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3959..4030,4432..4705,4780..5434,5496..5811,6015..6146,
                     6217..6831)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    7607..7640
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             10308..10334
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctagagtcga cctcgaacgt taacgttaac gtaacgttaa ctcgagctag atcataatca
       61 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      121 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      241 ctagttgtgg tttgtccaaa ctcatcaatg tatgtcgagg tcgacctgca gcccaagctg
      301 ctagagatcc actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
      361 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
      421 tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct
      481 cgccctcgaa accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg
      541 cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga
      601 cactagttct agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
      661 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      721 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      781 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      841 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     2821 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2881 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2941 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3001 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3061 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3121 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3181 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3241 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     3301 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     3361 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     3421 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacgt accgggcccc
     3481 tagtatgtat gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt
     3541 ttttttttac tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat
     3601 ccaagggtat ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac
     3661 tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc
     3721 gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct
     3781 tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta
     3841 aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga
     3901 ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat
     3961 gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca
     4021 tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact
     4081 gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc
     4141 gcaattgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata
     4201 taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta
     4261 tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa
     4321 aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt
     4381 tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc
     4441 ggagcggctt cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg
     4501 ctccggccac ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac
     4561 ctcacctatg cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga
     4621 tggcggcagc tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg
     4681 cccaggaaac atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga
     4741 aatcttaata aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg
     4801 gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc
     4861 ttgcctttcg atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg
     4921 gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc
     4981 tctttatcgg ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc
     5041 cacgacatct gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt
     5101 cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg
     5161 gcggagaaag gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga
     5221 tctgcgatga gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc
     5281 tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg
     5341 agctgtttga gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg
     5401 gcactcccag cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt
     5461 atctagtatt acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac
     5521 tacaatccgg cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag
     5581 tcccgtgatc ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat
     5641 atggagcagt tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg
     5701 tacacctaca aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg
     5761 tcggtgctca aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt
     5821 tccagtggaa acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta
     5881 gatagaattg cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata
     5941 gttttgcaga agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac
     6001 attttaattt gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca
     6061 cgcaagtggg tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct
     6121 ttcaaaacgt ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat
     6181 aatttactaa ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt
     6241 tttatgaggg aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg
     6301 attgccgaat tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg
     6361 atgatcggac tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg
     6421 gtggccaatg tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg
     6481 atggcgctgt ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc
     6541 ttgaactcgg gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt
     6601 tacgccaacg agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc
     6661 tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac
     6721 ttctccgccg ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc
     6781 cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat
     6841 atccgaaata actgcttgnt tttttttttt taccattatt accatcgtgt ttactgttta
     6901 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     6961 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     7021 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     7081 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     7141 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     7201 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc
     7261 gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta
     7321 tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct
     7381 aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt
     7441 acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa
     7501 atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat
     7561 acatttaatt tagaaaatgc ttggatttca ctggaactag gctagcataa cttcgtataa
     7621 tgtatgctat acgaagttat gctagcggat ccgggaattg ggaattcgat atcaagcttg
     7681 cacaggtcct gttcgataac gtcgtactcg ggaatcacct ttaacttgtc cgctattggg
     7741 tatttgtcct taaacacatt cgtaatctct cccacaatca ttgccctggc ctctgcaatg
     7801 taaaaagtat ttataaaata tcgctggctc taaaggaaac atattaatca aagtattgag
     7861 gctttatcaa tctaacattt acaagtagtc agaccacaat ttttagactg cacttctgcg
     7921 taagtaaatg aaatgaaatg gtgacctact cttgggtagc cgtactgtta tatgctgacg
     7981 agccttcacc tgggcctgct ttttctcctg tttcgtctga tgcgtcggat gcggctggac
     8041 tggctgattg ttgggattgg gttttggatg ctgctccgaa aggtgggcca cagcttttgc
     8101 gatggtggtg cccactatgc ggttgctgca ggaactacag ctgaatgttc gctttattgt
     8161 ctgcagctcc tggggctgga cgaaggccac gccctcaccc ggtgcggcca tctcctgcgg
     8221 caaaacgtat gtgggattcc tgtccaagta gctctcgatg cccaccataa tgcgctccag
     8281 caggttggcc aggtgcgggt ctagctgcgg cggattcgtc gtctggttca ctgagtttag
     8341 ggtctgcaga aagaagtgtg cctccatggt aagcggattc atctgcacga tcagcgggct
     8401 ttagtctaaa aattcgaatg atacaaactc gtactcacgt aaagcagttt gtttggcatc
     8461 atctcggcca tcgtcttatt cttcttcgtg gcctttgtta ccggagcggc tatgttgggc
     8521 gcggtggagc ccggaagctg aaaggttaat cgaatgcgtt aacttttctg caactcgaaa
     8581 gtttgccgcc tttgttcgac tgccaataac tgttgattcg aaaattcgaa tcgaagcggt
     8641 tgaatttcgt agggtggcca actacaccaa agttcgccgg cggtgtatgc atgtgagtgt
     8701 gagtgtgaca gtgggtgtac gtgtgcttta agcgacacaa tcgcaccgcg atgtacatat
     8761 ttttgtagta atgtgtgtgc atttttcgtc cggatcacgg ctccataaaa taaatcatat
     8821 cacagggctc agggatttgt gagtagaagt tcccgccact taaacgtcac ttgtgaaatc
     8881 gccatcgacg gtcaactttt actcacactg gtctttcgcg gacggaccgt ctcaaagtac
     8941 tgcctttctg cgttggaaaa catcgccttt ttcgtccaaa aggagtcccc aggttcgatc
     9001 cgcatggcgt tgtgcgtgcg tgcctttctt ttcaaatgat tacggctatt aacttggggg
     9061 cgttaagttg gaaacacgta aattgcagac tgcgattaga gtgaccatga gtaggagttc
     9121 aaaatctcct gacatcattt tcttaaaacc tgctttgttt tttacatttc tatttaatat
     9181 aactcctatt tgaataaaaa aacaaaacaa gtttagatgt taagatatta actacatcct
     9241 ttgctccaaa gggagagggg aagttatgga gttaattaat ttgctgttgg aaatcaatat
     9301 ggagtcagaa atataatgat ttactaaacc ttattgaatc ggtaacgatg cgaatttata
     9361 ttaaaatagc ttttatgaaa cattcaacaa aaatattatt aatgttggcc cactttagca
     9421 accggttagg tctaccggtt gggcaagcaa agattcacgc cctggttcga gtcccaacta
     9481 gtcctgcaaa ataccgcagc aagttttaga gagaccaagt gccattacct ctcccacttc
     9541 agttatcggt tatgcggcgt ttaagtcgac agcttgccgt ctctagctcc ggtgcctata
     9601 taaagcagcc cgctttccac atttcatatt cgttttacgt ttgtcaagcc tcatagccgg
     9661 cagttcgaac gtatacgctc tctgagtcag acctcgaaat cgtagctcta cacaattctg
     9721 tgaattttcc ttgtcgcgtg tgaaacactt ccaataaaaa ctcctgcagg tgagtacttt
     9781 aaaaaaaaat ctagtgaaat aatgctgaaa agaaatttgt gtgggcaaaa ttcaatgggc
     9841 aaaaacgcga tgcggctttt tctcaaaatg gcggccggcc tgcgtttttt cctcaaaagt
     9901 gatgacgtca tgcctgtntt tttttttttg ttcgcaatga ggaatggctc ttaaaatcta
     9961 ctagataaaa aaaatattca ttatttctat gctgctggaa cgcttcatta atcttaaaaa
    10021 ttctaaattc ggttaccatg atacttcgac gcataactgt agattttgga tagaattaaa
    10081 gagaaaatgg cgagagagta aaattccggc gtcggcaaag tagagcaaaa aaatcagtat
    10141 accatttagc tacctctctc actcgcacgc agtgccggct caagttgggc gcggctctgc
    10201 aattatcgat tttcttgggg tgtgtaacta atcatccgtt ttcccttcct cctcatccac
    10261 agcgtgaagg tacccgcccg gggatcagat ccgcggccgc caccatgtac ccctacgacg
    10321 tgcccgacta cgcgatggat agcaaaatag aacctgcaga tgccataagc aaccagaaag
    10381 ctggtcttac ttcgggcaaa cctaaaaaag acaagtcagt caaggggacg tcgcaagcgg
    10441 ttgtttcaga caccaagaaa atggaggcca gaaagtcctc ctcggacacg gtgatcgagc
    10501 cattgatcaa gcagcaggcg ccactgcact cgtcaaagac cacgccaacg actttgacgg
    10561 cgaacttcgt gcaaaacatt cgattcgtcc gtaaaaatgt ggaacagtca ggcaggaaca
    10621 ccaatccact gcagttcgtt gaactcgaga cagagtttcc tcgtgactat gacgacaaca
    10681 tcgagatgct ttcccgcgag gcggagcatc tggaggagca gttccgcacg cctacgcgct
    10741 ccaatgctac ggatgccact tcccaccatg tggccggaat catcgacaac attataaccg
    10801 aagcgtcacg gagtcttaca atcgaaaaga ctgaagacga tgtgccattg aagtcttcta
    10861 ccaagcactc aagcggcgta aaacgagttg gctttcaagt agaggataag gacgataccg
    10921 aagttcagag cgaaaagcag gcaaagagct tcgacgacat aaccaaaaaa gcggagagct
    10981 ccgaggctag cgcagaggaa gctgctgtca ccgggtcctc gacagatgca tctgcatctc
    11041 ccctgccctc cacgtcgctg gtctccacca catcttcagc tacatcgata accaagagta
    11101 agagcgatga ggacgatgat ccggtggcca tgtcgccgtg cggacgattc tttaagtacg
    11161 ataaggaagt cggacgcggt tcctttaaaa ctgtctatcg tggcctggac acgttgactg
    11221 gtgttccagt tgcctggtgc gagttattgg acaagcaagt aaaaaaaagt gaacgtacta
    11281 gatttcgcga ggaggcagac atgctgaaga aacttcagca cccaaacata gtgcggtttt
    11341 atacatattg ggagtttcct attggccgca agaagaatat tgtactagtc accgaactaa
    11401 tgttatctgg aactttgaaa tcctatttga aacgattcaa aaaaatacac ccaaaggtat
    11461 tgaagtcatg gtgtcgccag atactaaaag gccttaactt cctgcatact cgtcaatttc
    11521 ctataattca tcgtgatttg aaatgtgata atatttttat aactggcacc actggtagtg
    11581 ttaaaatcgg cgacttgggc ctggcaactt tgaaaaaccg ctcccacgcg aaatctgtga
    11641 ttggtacgcc ggagtttatg gccccggaaa tgtacgagga gcactacgac gagtcggtgg
    11701 acgtatatgc attcggcatg tgcatgttgg agatggccat ttccgaatat ccttacagtg
    11761 agtgcaaggg tcctgcacag atctacaaaa aggtgatatc gggcattaag ccagcggcgt
    11821 tggctaaggt ggaggatccc aacgtgcgcg acattatcga acggtgcatt gaacttaaaa
    11881 aggaggatcg ccccagctgc aatgaactgc tagaatcgga gttctttgac gaagatatcg
    11941 gtatacgagt ggagccaact gcttcggagc agttcctttc tgatccgagc atcagcatca
    12001 tcgagttccg actgcggttt atggatccca agaagcgctc gtcccgtcat aaggaaaacg
    12061 aggcaattca gtttgagtac aacatcaggc atgacgagta cgaacagatt gcgcaagaaa
    12121 tgatgaagga aaatattata tctgaggacg actctcgcgc tgtagctcgg cttttaaaag
    12181 tgcaggtggt gtcgttgctt aaggaacgtg cccagcgtca aactcaaatc aagctgcaaa
    12241 acgagaagtc tcgtttggaa aaattggcat tgcaaaagca acgtgagagt ttgccgacta
    12301 acgtcgatga agacgaggaa gaggaggagg agtccgagga cgaggaggat ggtgttaaat
    12361 ggaatcagcg cctgcagcta agatatgacc tgcttaacac tgattcagaa acaagcctgg
    12421 ctctatccac gaacagcgtc gaaccgcagc agctgtccac tcgctcgaac acttcgattc
    12481 cgaacagtgg cattcagcaa cctgttcagg tcccaggtca agtgcctgtt tcccagttga
    12541 tctcggtgca accacaggct attccgtcac cggctattcc catgcaacag aagccgacgg
    12601 tgcactacat tcaaccgcca cagctggcct cttatcaaaa ttcgaatgca tcgatgcagg
    12661 agatgaccaa taaccaggtc atttcgccga caggcagtca acaaatgcaa cagcaacaac
    12721 ccgtagtggc accaacagtc aatcatcagg tcatgccgca gcagcaagtg aaccaacaac
    12781 agcaacaacc acaaatgatg cagcaaatac cccagcaagt tcaagtgcaa cagccccaaa
    12841 ctgtattgcc tccgcagcca catgagcagc agccacagca acaacaacag ccactgcagc
    12901 agcaactgca gcagcttatg catactaacg tgcaggctcc ggatttgact caacagcaac
    12961 aaatggctca gcaacaggcc cagcaatatt ttcagcagca gcagcaacag cctcagcagg
    13021 ccgttaacat gcaacaagct tatgcaatgc agcaagctgg ccaacagcaa cagctttcgc
    13081 agcctcttca aattcagcaa cagatcttgc agcaacaaca agtggccgtt tcacatcagc
    13141 aacagataat gcagcaacaa ctggcccagc atcagctgca acagctgcag cagcagcagc
    13201 ttcaacaaca gcaactgcaa cagcaacagc agcagcaaat acaacaacag caactccaac
    13261 aacaacaact ccagcaacag caattcgtac agcagtatgc gcaggcaatg cctcagcaac
    13321 aacatcagca actcgttacg ggatcgcaag tcatggcacc gcatcaacat cagcaaccta
    13381 ttcagatccc agtgcagatg caagttccac cgacgtccgt ggctcctcca atacaacaca
    13441 cttacaatca gcagggcgga caagtaacgc tcagtgatgc ccaacagcaa cagcatcctg
    13501 gattctcggc cgtaccacaa caggcagcgc catttataca acagcccaca cagcaaccca
    13561 ttcagctatc aatgcctttg gaacaacaat tgcagcaatt gctgcatagc cagccagccc
    13621 agcaacaaca ggccatgagc caacagcagc aacagtctat ggtccaacag cagcaacagc
    13681 ccttggtgca acagcaacaa ctgcctttgg tacaacagca gccgcctttg gtccagcagc
    13741 agcaaccgct ggttcagcat cagcagcctt cggtccaaca tcagcaacct ttggtgcaac
    13801 agccccagca acagcaacca cagccccaga atcagcaacc acagccccag acacagcaaa
    13861 cacacgtggt tcagcaacaa ccaccacagc agcaacctgc agtagagcaa atctcacaga
    13921 tttcttcgca agtgcctgtt caacaggaaa atctgcagcc aatacaggtc aacaaagatg
    13981 cgaatgtcgc aacggatgca atgtccctaa atagcgccca cggtgccctc gaacctgcac
    14041 cgaagaccga accccagaac tctgctgatg cggaaaagca gcaaaagcag acaggcacgg
    14101 gcacacgttc ccagaaaccg aggcgatcaa accggagcgg caacgaacgg attcccaaac
    14161 ttagtgtaac cagcgtcgac gagggcagcg tcataaactg tcacatggaa aataaactta
    14221 aaacgattac atttaaattc gacataggag acgtaaatcc ggttgaaata gccaataaat
    14281 tgatcgccca agatttgctt tctaattgtc aaagtacagt ttttgtggaa atgatcaatg
    14341 aaattgttga ccaagtcaaa cagaatccca atcaaatccc tataccgaca aattatcgcc
    14401 gcaatattga aaagcgggat gaaaccgcca gcgacatcac aaagatgttc gagcccacta
    14461 tccatggtgt tgaaaccctg ccatccggtg gaggaggcgc tgaacagtca aactgcaact
    14521 tgactttaga ggccaggtcc catttatcca acataccaaa tgcgaaagaa ccacagctga
    14581 acgtcagcac tccgcccaca accacttcga ctatgtcttc ctcatcaacc gcatcgaggg
    14641 atgcgcccaa tagtagcaat gacgtgacca tcggctctgg ttccgtttct cgaaaaacga
    14701 gcacggcctc ggagtacact tcgctatcga ttgactatat gcccgacagc aacattactc
    14761 caactggtcc ggagccaccg cttgacgatg gcaaggataa aaaaccttgc tcccttgcta
    14821 tagccagaat gcaaaagctc ttggagtcgg ccggcaatgg tgctcctcgc agcctgaatt
    14881 tgcccttgaa tcgtcacctg aaaattcaag aggacttgaa gcatactaga agtcttgacg
    14941 atttgaccgc agtgaaaata acctttgaca tgcccaacaa ggctgcattg gacacttcgg
    15001 aaaacgctca gcaggccaca gaagcggaag cggagaaacc aaaagataaa tccggacagg
    15061 cagtaggcaa ccagggaact ggagcggcca acactctaga gcagttaaaa atagaactgg
    15121 agaacattac tcatgcccac gcgtttgcct ccgctgtagt ggcctcgatt aacaacagag
    15181 ctcctcatca agcttcccca gcaatgtcct ccctaaaggc cacttctgga caaccacaga
    15241 cacaagagat aaccaagcca aataacggag caggcccttc agtaccctca gtgggtcaga
    15301 atactcccac tgcagcctta acatccgcca ggggctcagg ctcctccgtc tacaactcac
    15361 gtcgcacatc catcgacaac agcgtgggat ccgacatgca cctgcacact gccactaatc
    15421 ttgaagggac agtgagcaac ccggatccga tcccgactga ggcatcggtt ggaattacca
    15481 ttgctacggg tcatgagaag caactctcca agcagcccag tttggaaaag ccttctgcca
    15541 cttccatttt aacgaacaac agtgatcctc cgcaaaggaa tcccagcaat ggatcaatta
    15601 accaaaattc aatagctgat ttggaaaaga aattggcagc tttgcgaaat accgaaaaca
    15661 cagaagagtc tgcaacagca acgttgtcag tggttcctaa acaagttgaa gaggtagtca
    15721 atccaagcgc gcgtaaaatt tcgcgtttta gtgtcagtcg tgtgcaggag cagaaaactt
    15781 caaccggagt tgaggaacca gctcaaggtc aacttaagat cgacctccaa gtcgccggcc
    15841 ctggatgata acctaggtgg aggccaggtg cagaccaaca actccgttca gaacggcagc
    15901 gtggtcaaca ctccaaccga agtcatcagc tctcctattc aaaatgttcc gctggccatt
    15961 aacggaattc agttgatgta ccaacagccc cagcagatac accagttgcc aggtggaaca
    16021 tcaactacta caagtggagc agggactcag tgcattgtag tgccgcaagt ggtcaatcaa
    16081 aatggcaccc acgtgcaaca gccgtctaac ctacagccac agcagcagag cgtacatcca
    16141 aatatgactc aacagccaca gcagacgcca cttaatgggc acccatctat ggttaacact
    16201 cttcagcagc agccaccaca acaatcctta ccgatgcaga cgattcagtc gcagcagcag
    16261 cagcataacc aaatgcctat catttcgcaa caacagcagc agcagatcct aatgcagcag
    16321 caacagcaac agggatccca gcaaggatca cagcaattta atttgcctgg cacacaacag
    16381 actcatcctc agcaccagtt catccagtcg cagcccaacc aacttcactc gcttccgccc
    16441 caggtagtga gcatggctca gggaaatctg ccgcatcagc ttccacccat gcagactatg
    16501 ggagcccaac agcaaatgat ctcgcaacaa cagcatcagt tgcagatgca gtcgcatatg
    16561 caccaacaaa acgtgcccgg aatgtacaac cagcagactg gagctcgtgt ggcagcaccc
    16621 caaaatttcg caggtgcagt tccaaatcat ttgttgcagc agtccccttt gatggcatcc
    16681 cagcagccag cacagcccat gcaacacgtt atgcaaccga ttttcaatgc aacgtccggt
    16741 gaagttaccg aagagcctgt ctctttagca gctacgcatc cgcatctgct accgagcgat
    16801 attcaatccg atataaagca caatttagac tcattagtga accaattgtg caatacgcgc
    16861 ttgggtacta accagcacca acggctgttg ctgttgcgtc agagacaact catcgaagag
    16921 gacgagctgc gtcttaaaca ttatgtggaa tacgaaaagt tccaaaaggc actgcgccaa
    16981 tctattagca caaacgttcc agctaatgca gcttactatt ctgcggctgc cgcacagctt
    17041 ccaactaatt tggcagcccc agcagctccg tcctcatcga atccgacgtc cactagctcc
    17101 gcaaacacat aac
//
