LOCUS       sequence-403375-        8152 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Luciferase reporter plasmid for Igf1 enhancer 1 containing only the 
            upstream AP1 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Roethler O, Zohar E, Cohen-Kashi Malina K, Bitan L, Gabel HW, 
            Spiegel I
  TITLE     Single genomic enhancers drive experience-dependent GABAergic 
            plasticity to maintain sensory processing in the adult cortex.
  JOURNAL   Neuron. 2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026.
  PUBMED    37354902
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026."
COMMENT     Sequence Label: pGL4.11_Nued2_E1_dAP2
FEATURES             Location/Qualifiers
     source          1..8152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             490..609
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    661..782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(672..691)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     726..745
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1030..1047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1201..1789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1281..1300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1989..2780,2781..2849))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2954..3002
                     /note="synthetic polyadenylation signal"
     misc_feature    3016..3107
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3056..3075
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      6983..6992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6989..8152,1..486)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
       61 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
      121 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
      181 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
      241 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
      301 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
      361 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
      421 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
      481 gccgtgaatt ctcacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
      541 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
      601 atcaacgtct aaggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga
      661 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      781 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      841 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
      901 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat aactatcgtc
      961 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     1021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1921 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     1981 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     2041 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     2101 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     2161 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     2221 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     2281 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     2341 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     2401 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     2461 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     2521 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     2581 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     2641 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     2701 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     2761 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     2821 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     2881 tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta
     2941 ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     3001 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     3061 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctggc ctaactggcc
     3121 ggtacctgag ctcgctagct tcctggcttg tagtgacctc gtctattgct agaaatcaga
     3181 aggtaccact gagccttcat ttgtttggtg aaatcatgga gttacattaa caagccgttt
     3241 ccccaaacta tggctctcta cccacttgta atacacccgt gcacaggaac attttctgaa
     3301 agtcaatgtt tcctatcact tgctccaaaa ttaaaccaga gatacatcat tcaggaagaa
     3361 gccagatgca ctgaagaaag aaaaacctgc tttatcagaa ggggcaagct tctctcacat
     3421 cccatagcaa tttaaattca tttgccggaa tgttaaatac aaccagtctt ttagttgggt
     3481 cccttcacat aaacaccaag ttagcataaa tgcaaaatag ttcacatgat taaatcttta
     3541 taacaccata atttatctta tgtacacaca cacacacaga gaaagagaga gagagagaga
     3601 gagagagaga tcttttactt gctgatactt ctacattttt tctggagttc tcaactctca
     3661 tcatctctct aaggaagctg gcttgtattg ccccagactc ttagtgtatt tagttctaaa
     3721 ggatagtata cagaataaag ggaaatttta attttgtttt taataggacc tatataattc
     3781 aggcctagct ctggtggaga gaaggggagg agaggtccat tagcatttat tgtgcacctg
     3841 ctttatgcta aacatgtctg gaaacagtgt ctcacacttc actctacatc ccacccactg
     3901 ctcctctcct attcaccctt tcccacaatc tttcccccat ccccctagcc ccagcaaagg
     3961 tatccatcat tggctgaaaa gtatacattt ttcagcatgg agcattactg ctgacaaaaa
     4021 cccactttag gaagtaagat ttcttctctt attcatagaa tgattcatgt cacacacact
     4081 tcttttttga ctcacacggt acgtcacaac aggctttgta aggcttataa ttactgaggc
     4141 tatttttgtg tctctattca ttgctttccc ccacccgcct tctctgcatt aataaccagt
     4201 ccatcccccc cccctcatta ttcagtacgc agcaaaatag aactggtgtc agccaatcag
     4261 cctcttgtat ggtttttagg cagaagctta aaaacattgg tccttgtcaa cagaaattaa
     4321 gcctgctgct accctattcc tgaggtagat ccaggaaaac tctgaaatag aatgggaaca
     4381 gatctcatat ctaattagat cctttctatc tctgagaatt gtattcagtg caatcattac
     4441 tgccacctgc ttgacagtgt gtacaatact ttgtttaagc ttccccacta ctggatccat
     4501 ttaacagacc aaaccactga ggaagcgcgt ttaaacgtgt ggcgcgccag ctagtaacag
     4561 ttggcattgg gactagaagg cctcattcct aaaccagcag atttttccat tgtaccagga
     4621 aaacaaaact gcaaatagca taagtcctct gattttactg tctccagaga aaatcatatc
     4681 ctcaatgcct gggtctttcc atctggaatt tggcaactct gctgatccct tctcatagtg
     4741 ggaactcttg taggaagtct aagagcaatt attttgggga tatatgctat ttttatgtct
     4801 tcttgtacat caccttcttt ggtcaggagg aaattagaat tctggaaata cgtttaatta
     4861 ttaaaaggac aagccttggc ttcaagtagt tgcaaatatg aattattagt ccatgtcatg
     4921 aaagggctgg agaactacaa agaaacaatg ataatctatt tgtcacaccc aattaagatc
     4981 tgccctggcc tgggagtaaa agagcattaa atctgtgagt ggtaacttca agcagcatgt
     5041 ctatatttgg gtaattctga acatgcctgg atttggtacc ttacagcatc ctctaaaaat
     5101 gtactacttt agaaagtgtt ttgagatttt gttgccttcg tgattgcagt ttgtgtgtgt
     5161 gtgtgtgtgt ggggggtgtt atttgagctt ttgagtttcc atgtagccaa agaaagaaag
     5221 tctaggaaga aatggtagac tcaaggtcaa attcaacaag gggaaacttc tttgtggtga
     5281 gtctagacaa caaagccatt agcccagtgt tcacccccct gcctacaccc taccttttcc
     5341 attgatgaat attcttacct gagccatcct cgcttctgag tcctgctctg ggtcctgtgg
     5401 agactgaata tcctgtctgt agtttttaat ccttgaagag atttttcatg aatagaactt
     5461 tccttataat ttagcaacac tgtccttgaa aacccccaca actatttaca taacagaagc
     5521 tattgtttga agtgcaaaac aggtcagaat ttggttccca gagattaatc actgcagtct
     5581 attttgtatt atcataataa tttcatttat tttgatacaa tttgttttca gaatcaaccc
     5641 cagcttcttt gcacaagtga caaatccatt tgtttctggt agaatgggta atgtgattaa
     5701 tgaccttgca acaggatttc tttaaaaagg gggaactttt tttttttcat ttttaattca
     5761 cagtaaacca gctgagtctg ttaagtgtgt aactgttctg aacttgtaag ccatgggtaa
     5821 gattttgaaa taccccctcc caatcttttg cggggtttct gggtattaat attaggcttt
     5881 ctccaagaat ctgatgatac tagtctccag gtgtaaagtg gacttgttgg attcccgcat
     5941 taaaaagaac ggggccccag tctttgccat ggccccatca tgtttattcc ggctggatgt
     6001 tctcatcaaa ccgaggtcat gcgaaataaa taaggtcatg cctagaagga tattgtcccc
     6061 gcacaagaac cttcgagtga actcgtgact actgcctcct ggtcttctgt gtgggcatcc
     6121 ccttctttct tgcagcatcc aggacccact tctgtccctg ctggtctgtt tctttctttc
     6181 tttctttctt tctttctttc ttgttttctt ccgtttttcc ccttgagttc tctggggttg
     6241 ggcctgggat tttgggtacg cctacagact gtccgcctct gcaatcgacc gtcgtgcttc
     6301 ccgcgccaga acaaggaagc ccacgcgagc cgcgtaggtg gctcgcgtgg aagacccggg
     6361 ttcgagtccc gctgggagaa ttcctcctag gcaagtggag tcatctcggt caggatttct
     6421 cagccggaag ccgcgccaac acctctcatc gtgggcggca agttgtcccc ggggcttctt
     6481 tgaccgtgcg gggccagccg acgctttctc tgaccgtttc cgaagcccag tttcctttcc
     6541 taaggagcct tggggatcct gcactcgcgt ctctgaaacc caacccacga cagaacccag
     6601 ccgcctgggc cagcttagca atgcgcccgc gccccgccct ccgcgtgacg cgaacgccgg
     6661 cggaccaatc agagagcgcg gcgcgagccc cacgcgcctg tggccccacc ctccaagctt
     6721 aagaaagggc gcgcgggcag agcccgggcc agaacgccgc gcaaagcgga gagcgggttt
     6781 ttgcgacggc aggcggcgct tgtgctgccg tttccgtgct gcacgcggtt ccctcgagtg
     6841 ccgcgctcgg acagacgtag ccggccgcga aggcgcccag acggcaaacc agcgacccat
     6901 gctgaagtga gcacacaggt cagcgaggat atcaagatct ggcctcggcg gccaagcttg
     6961 gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc
     7021 agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa
     7081 gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat
     7141 tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg
     7201 gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc
     7261 cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga
     7321 gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa
     7381 agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat
     7441 catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca
     7501 tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac
     7561 catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc
     7621 gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat
     7681 catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac
     7741 cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga
     7801 gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact
     7861 atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga
     7921 gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt
     7981 ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat
     8041 cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc
     8101 taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg gc
//
