LOCUS       sequence-403565-        8160 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Luciferase reporter plasmid for Igf1 enhancer 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Roethler O, Zohar E, Cohen-Kashi Malina K, Bitan L, Gabel HW, 
            Spiegel I
  TITLE     Single genomic enhancers drive experience-dependent GABAergic 
            plasticity to maintain sensory processing in the adult cortex.
  JOURNAL   Neuron. 2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026.
  PUBMED    37354902
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026."
COMMENT     Sequence Label: pGL4.11_Nued2_E1
FEATURES             Location/Qualifiers
     source          1..8160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             490..609
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    661..782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(672..691)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     726..745
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1030..1047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1201..1789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1281..1300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1989..2780,2781..2849))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2954..3002
                     /note="synthetic polyadenylation signal"
     misc_feature    3016..3107
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3056..3075
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      6991..7000
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6997..8160,1..486)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDKVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
       61 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
      121 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
      181 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
      241 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
      301 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
      361 gccaagaagc tgcgcggtgg tgttgtgttc gtggacaagg tgcctaaagg actgaccggc
      421 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
      481 gccgtgaatt ctcacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
      541 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
      601 atcaacgtct aaggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga
      661 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      781 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      841 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
      901 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
      961 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     1021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1921 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     1981 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     2041 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     2101 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     2161 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     2221 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     2281 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     2341 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     2401 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     2461 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     2521 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     2581 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     2641 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     2701 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     2761 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     2821 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     2881 tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta
     2941 ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     3001 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     3061 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctggc ctaactggcc
     3121 ggtacctgag ctcgctagct tcctggcttg tagtgacctc gtctattgct agaaatcaga
     3181 aggtaccact gagccttcat ttgtttggtg aaatcatgga gttacattaa caagccgttt
     3241 ccccaaacta tggctctcta cccacttgta atacacccgt gcacaggaac attttctgaa
     3301 agtcaatgtt tcctatcact tgctccaaaa ttaaaccaga gatacatcat tcaggaagaa
     3361 gccagatgca ctgaagaaag aaaaacctgc tttatcagaa ggggcaagct tctctcacat
     3421 cccatagcaa tttaaattca tttgccggaa tgttaaatac aaccagtctt ttagttgggt
     3481 cccttcacat aaacaccaag ttagcataaa tgcaaaatag ttcacatgat taaatcttta
     3541 taacaccata atttatctta tgtacacaca cacacacaga gaaagagaga gagagagaga
     3601 gagagagaga tcttttactt gctgatactt ctacattttt tctggagttc tcaactctca
     3661 tcatctctct aaggaagctg gcttgtattg ccccagactc ttagtgtatt tagttctaaa
     3721 ggatagtata cagaataaag ggaaatttta attttgtttt taataggacc tatataattc
     3781 aggcctagct ctggtggaga gaaggggagg agaggtccat tagcatttat tgtgcacctg
     3841 ctttatgcta aacatgtctg gaaacagtgt ctcacacaac ggttcactct acatcccacc
     3901 cactgctcct ctcctattca ccctttccca caatctttcc cccatccccc tagccccagc
     3961 aaaggtatcc atcattggct gaaaagtata catttttcag catggagcat tactgctgac
     4021 aaaaacccac tttaggaagt aagatttctt ctcttattca tagaatgatt catgtcacac
     4081 acacttcttt tttgactcac acggtacgtc acaacaggct ttgtaaggct tataattact
     4141 gaggctattt ttgtgtctct attcattgct ttcccccacc cgccttctct gcattaataa
     4201 ccagtccatc ccccccccct cattattcag tacgcagcaa aatagaactg gtgtcagcca
     4261 atcagcctct tgtatggtga ctcattttta ggcagaagct taaaaacatt ggtccttgtc
     4321 aacagaaatt aagcctgctg ctaccctatt cctgaggtag atccaggaaa actctgaaat
     4381 agaatgggaa cagatctcat atctaattag atcctttcta tctctgagaa ttgtattcag
     4441 tgcaatcatt actgccacct gcttgacagt gtgtacaata ctttgtttaa gcttccccac
     4501 tactggatcc atttaacaga ccaaaccact gaggggcgcg ctacggcgcg ccagctagta
     4561 acagttggca ttgggactag aaggcctcat tcctaaacca gcagattttt ccattgtacc
     4621 aggaaaacaa aactgcaaat agcataagtc ctctgatttt actgtctcca gagaaaatca
     4681 tatcctcaat gcctgggtct ttccatctgg aatttggcaa ctctgctgat cccttctcat
     4741 agtgggaact cttgtaggaa gtctaagagc aattattttg gggatatatg ctatttttat
     4801 gtcttcttgt acatcacctt ctttggtcag gaggaaatta gaattctgga aatacgttta
     4861 attattaaaa ggacaagcct tggcttcaag tagttgcaaa tatgaattat tagtccatgt
     4921 catgaaaggg ctggagaact acaaagaaac aatgataatc tatttgtcac acccaattaa
     4981 gatctgccct ggcctgggag taaaagagca ttaaatctgt gagtggtaac ttcaagcagc
     5041 atgtctatat ttgggtaatt ctgaacatgc ctggatttgg taccttacag catcctctaa
     5101 aaatgtacta ctttagaaag tgttttgaga ttttgttgcc ttcgtgattg cagtttgtgt
     5161 gtgtgtgtgt gtgtgggggg tgttatttga gcttttgagt ttccatgtag ccaaagaaag
     5221 aaagtctagg aagaaatggt agactcaagg tcaaattcaa caaggggaaa cttctttgtg
     5281 gtgagtctag acaacaaagc cattagccca gtgttcaccc ccctgcctac accctacctt
     5341 ttccattgat gaatattctt acctgagcca tcctcgcttc tgagtcctgc tctgggtcct
     5401 gtggagactg aatatcctgt ctgtagtttt taatccttga agagattttt catgaataga
     5461 actttcctta taatttagca acactgtcct tgaaaacccc cacaactatt tacataacag
     5521 aagctattgt ttgaagtgca aaacaggtca gaatttggtt cccagagatt aatcactgca
     5581 gtctattttg tattatcata ataatttcat ttattttgat acaatttgtt ttcagaatca
     5641 accccagctt ctttgcacaa gtgacaaatc catttgtttc tggtagaatg ggtaatgtga
     5701 ttaatgacct tgcaacagga tttctttaaa aagggggaac tttttttttt tcatttttaa
     5761 ttcacagtaa accagctgag tctgttaagt gtgtaactgt tctgaacttg taagccatgg
     5821 gtaagatttt gaaatacccc ctcccaatct tttgcggggt ttctgggtat taatattagg
     5881 ctttctccaa gaatctgatg atactagtct ccaggtgtaa agtggacttg ttggattccc
     5941 gcattaaaaa gaacggggcc ccagtctttg ccatggcccc atcatgttta ttccggctgg
     6001 atgttctcat caaaccgagg tcatgcgaaa taaataaggt catgcctaga aggatattgt
     6061 ccccgcacaa gaaccttcga gtgaactcgt gactactgcc tcctggtctt ctgtgtgggc
     6121 atccccttct ttcttgcagc atccaggacc cacttctgtc cctgctggtc tgtttctttc
     6181 tttctttctt tctttctttc tttctttctt gttttcttcc gtttttcccc ttgagttctc
     6241 tggggttggg cctgggattt tgggtacgcc tacagactgt ccgcctctgc aatcgaccgt
     6301 cgtgcttccc gcgccagaac aaggaagccc acgcgagccg cgtaggtggc tcgcgtggaa
     6361 gacccgggtt cgagtcccgc tgggagaatt cctcctaggc aagtggagtc atctcggtca
     6421 ggatttctca gccggaagcc gcgccaacac ctctcatcgt gggcggcaag ttgtccccgg
     6481 ggcttctttg accgtgcggg gccagccgac gctttctctg accgtttccg aagcccagtt
     6541 tcctttccta aggagccttg gggatcctgc actcgcgtct ctgaaaccca acccacgaca
     6601 gaacccagcc gcctgggcca gcttagcaat gcgcccgcgc cccgccctcc gcgtgacgcg
     6661 aacgccggcg gaccaatcag agagcgcggc gcgagcccca cgcgcctgtg gccccaccct
     6721 ccaagcttaa gaaagggcgc gcgggcagag cccgggccag aacgccgcgc aaagcggaga
     6781 gcgggttttt gcgacggcag gcggcgcttg tgctgccgtt tccgtgctgc acgcggttcc
     6841 ctcgagtgcc gcgctcggac agacgtagcc ggccgcgaag gcgcccagac ggcaaaccag
     6901 cgacccatgc tgaagtgagc acacaggtca gcgaggatat caagatctgg cctcggcggc
     6961 caagcttggc aatccggtac tgttggtaaa gccaccatgg aagatgccaa aaacattaag
     7021 aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa
     7081 gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag
     7141 gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag
     7201 cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc
     7261 ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc
     7321 tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg
     7381 agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag
     7441 atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg
     7501 acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg
     7561 gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta
     7621 gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc
     7681 aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc
     7741 atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc
     7801 gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg
     7861 cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac
     7921 ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc
     7981 aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc
     8041 attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc
     8101 ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc
//
