LOCUS       sequence-404181-       10231 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2618
FEATURES             Location/Qualifiers
     source          1..10231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6088..6676
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6141..6161)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6559..6570)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6748..6981
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7059..7180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7096..7115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7150..7169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7220..7355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7375..7394)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7376..7394)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7404..7421)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7404..7420)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7413..7435)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7562..8017
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7649..7668)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7859..7880
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8043..8147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8148..8216,8217..9008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8366..8385)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9179..9767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9668..9687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9921..9938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10055..10076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10091..10096,10097..10114,10115..10121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10129..10145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10134..10156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10153..10169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10153..10169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10188..10208
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10190..10208
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc tccaggcttc cgacggcctg
     4141 gtcctgtgaa ccagtgacct ttgaagatgt aacactgggt tttaccccgg aagagtgggg
     4201 actgctggac ctcaaacaga agtccctgta cagggaagtg atgctggaga actacaggaa
     4261 cctggtctca gtggagtatc ctgagctcca gctggaccct aaattggatc ctcttcctgc
     4321 tgagagtccc ctaatgaaca ttgaggttgt tgaggtcctc acactgaacc aggaggtggc
     4381 tggtccccgg aatgcccaga tccaggccct atatgctgaa gatggaagcc tgagtgcaga
     4441 tgcccccagt gagcaggtcc aacagcaggg caagcatcca ggtgaccctg aggccgcgcg
     4501 ccagaggttc cggcagttcc gttataagga catgacaggt ccccgggagg ccctggacca
     4561 gctccgagag ctgtgtcacc agtggctaca gcctaaggca cgctccaagg agcagatcct
     4621 ggagctgctg gtgctggagc agttcctagg tgcactgcct gtgaagctcc ggacatgggt
     4681 ggaatcgcag cacccagaga actgccaaga ggtggtggcc ctggtagagg gtgtgacctg
     4741 gatgtctgag gaggaagtac ttcctgcagg acaacctgcc gagggcacca cctgctgcct
     4801 cgaggtcact gcccagcagg aggagaagca ggaggatgca gccatctgcc cagtgacagt
     4861 gctccctgag gagccagtga ccttccagga tgtggctgtg gacttcagcc gggaggagtg
     4921 ggggctgctg ggcccgacac agaggaccga gtaccgcgat gtgatgctgg agacctttgg
     4981 gcacctggtc tctgtggggt gggagactac actggaaaat aaagagttag ctccaaattc
     5041 tgacattcct gaggaagaac cagcccccag cctgaaagta caagaatcct caagggattg
     5101 tgccttgtcc tctacattag aagatacctt gcagggtggg gtccaggaag tccaagacac
     5161 agtgttgaag cagatggagt ctgctcagga aaaagacctt cctcagaaga agcactttga
     5221 caaccgtgag tcccaggcaa acagtggtgc tcttgacaca aaccaagttt cgctccagaa
     5281 aattgacaac cctgagtccc aggcaaacag tggcgctctt gacacaaacc aagttttgct
     5341 ccacaaaatt cctcctagaa aacgattgcg caaacgtgac tcacaagtta aaagtatgaa
     5401 acataattca cgtgtaaaaa ttcatcagaa gagctgtgaa aggcaaaagg ccaaggaagg
     5461 caatggttgt aggaaaacct tcagtcggag tactaaacag attacgttta taagaattca
     5521 caaggggagc caagtttgcc gatgcagtga atgtggtaaa atattccgga acccaagata
     5581 cttttctgtg cataagaaaa tccataccgg agagaggccc tatgtgtgtc aagactgtgg
     5641 gaaaggattt gttcagagct cttccctcac acagcatcag agagttcatt ctggagagag
     5701 accatttgaa tgtcaggagt gtgggaggac cttcaatgat cgctcagcca tctcccagca
     5761 cctgaggact cacactggcg ctaagcccta caagtgtcag gactgtggaa aagccttccg
     5821 ccagagctcc cacctcatca gacatcagag gactcacacc ggggagcgcc catatgcatg
     5881 caacaaatgt ggaaaggcct tcacccagag ctcacacctt attgggcacc agagaaccca
     5941 caataggaca aagcgaaaga agaaacagcc tacctcatag taatgaacta gtccgtaact
     6001 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa tcatcagtcg
     6061 cgacaaagat tcacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6121 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6181 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6241 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6301 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6361 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6421 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6481 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6541 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6601 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6661 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     6721 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     6781 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6961 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     7021 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7081 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7201 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7261 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7321 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7381 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7441 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7501 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7561 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7621 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7681 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7741 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7801 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7861 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7921 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7981 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8041 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8101 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8161 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8221 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8281 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8341 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8401 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8461 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8521 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8581 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8641 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8701 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8761 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8821 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8881 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8941 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9001 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9061 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9121 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9301 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9541 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9781 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9961 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    10021 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10081 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10141 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    10201 actaaaggga acaaaagctg gagctgcaag c
//
