LOCUS       sequence-404323-       12325 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12325)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3231
FEATURES             Location/Qualifiers
     source          1..12325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4118..4142
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7998..8022)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8030..8071
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8182..8770
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8235..8255)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8653..8664)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8842..9075
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9153..9274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9190..9209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9244..9263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9314..9449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9469..9488)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9470..9488)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9498..9515)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9498..9514)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9507..9529)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9656..10111
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9743..9762)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9953..9974
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10137..10241
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10242..10310,10311..11102)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10460..10479)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11273..11861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11762..11781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12015..12032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12149..12170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12185..12190,12191..12208,12209..12215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12223..12239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12228..12250
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12247..12263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12247..12263
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12282..12302
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12284..12302
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagttgg
     4141 caccatgcat aagaagaggg ttgaagaggg ggaggcctca gacttctcgc tggcctggga
     4201 ttcctccgtg acagcagcag gaggcctaga aggagagcca gagtgcgatc agaaaaccag
     4261 ccgtgcgctg gaagacagga acagcgtgac aagtcaagag gagagaaatg aggatgatga
     4321 agacatggag gatgaatcaa tttacacctg cgatcactgt cagcaggact tcgagtctct
     4381 ggcagacctg acggaccacc gggcccaccg ctgtcctgga gatggtgatg acgacccaca
     4441 actctcctgg gtggcctcgt ctccctccag caaggatgtt gcgtcaccca cgcagatgat
     4501 cggagatggt tgtgacctcg gcctcggcga ggaggaaggg ggcacgggcc tgccataccc
     4561 ttgccagttc tgcgacaagt ccttcatccg cttgagctac ttgaagaggc acgagcagat
     4621 ccacagcgac aagctgccgt tcaagtgcac ctactgcagc cgcctcttca agcacaagag
     4681 gagccgcgac cggcacatca agctgcatac gggcgacaag aagtatcact gccacgagtg
     4741 cgaggcagcc ttctcccgca gcgaccacct caagatccac ctgaagaccc acagctccag
     4801 caagcccttc aagtgcactg tgtgcaagcg cggcttctcc tccaccagct cgctgcagag
     4861 ccacatgcag gcccacaaaa agaacaagga gcatctggcc aagtcggaga aggaagccaa
     4921 gaaggacgac ttcatgtgcg actactgcga ggacaccttc agccagacgg aggagctgga
     4981 gaagcacgtg ctcacccgcc acccgcagct gtccgagaag gcggacctgc agtgcattca
     5041 ctgccctgag gtcttcgtcg acgagaacac actgctcgcc catatccacc aagcccacgc
     5101 caaccagaaa cacaagtgcc ccatgtgccc tgagcagttc tcctcagtgg aaggtgtcta
     5161 ctgccacctg gacagccacc ggcagcccga ctccagcaac cacagtgtca gtcccgaccc
     5221 tgtactgggc agcgtggcct ccatgagcag cgccacaccc gactccagcg cctctgtgga
     5281 gcgtggctcc accccggact ccaccttgaa gccgctgcgg gggcagaaga agatgcggga
     5341 tgacgggcag ggctggacca aggtggtcta tagctgcccc tattgttcca agcgggactt
     5401 taacagcctg gccgtgctgg agatccacct gaagaccatc cacgcggaca agccccagca
     5461 gagccacaca tgtcagatct gcctggactc catgcccacc ctctacaacc tcaacgagca
     5521 cgttcgcaag ctgcacaaga accatgccta ccctgtgatg cagtttggca acatctctgc
     5581 cttccactgc aactactgcc ccgagatgtt cgccgacatc aatagcctgc aggagcacat
     5641 ccgcgtctcc cactgcggcc ccaacgccaa cccctctgac ggtaataatg ctttcttctg
     5701 caaccagtgc tccatgggtt tccttactga gtcctccctc accgagcaca tccagcaggc
     5761 ccactgcagt gtgggcagtg ccaaactaga gtctccggtg gtgcagccca cgcagtcctt
     5821 catggaggtc tattcctgcc cctactgcac caactccccc atctttggct ccatcctgaa
     5881 actcaccaag cacatcaagg agaaccacaa gaacattcca ctggcccaca gcaagaagtc
     5941 caaggccgag cagagcccag tctcgtccga tgtggaggtg tcttccccga agcggcagcg
     6001 gctctcagca agcgccaact ccatctccaa tggggagtat ccttgtaatc aatgcgacct
     6061 caagttctcc aactttgaga gcttccagac ccacctgaag ctgcacctgg agctgctgct
     6121 gcggaagcaa gcgtgccccc agtgcaaaga ggactttgac tcccaggagt ccctcctgca
     6181 gcacctgaca gtgcattaca tgaccacgtc gacccactat gtgtgcgaga gctgcgacaa
     6241 gcaattttcc tcggtggatg acctgcagaa gcacctgctg gacatgcaca cctttgtgtt
     6301 gtaccactgc accctgtgtc aggaggtctt cgactccaag gtgtccatcc aggtgcacct
     6361 ggcggtgaag cacagcaatg agaagaagat gtaccgctgc acggcctgca actgggactt
     6421 ccgcaaggag gctgacctgc aggtgcacgt caaacacagc cacctgggca acccggccaa
     6481 ggctcacaag tgcatcttct gtggggagac cttcagcacc gaggtggagc tgcagtgcca
     6541 catcaccaca cacagcaaga agtataactg taagttctgc agcaaggcct tccacgccat
     6601 catcctgctg gagaagcacc tgcgggagaa gcactgtgtg tttgatgctg cgaccgagaa
     6661 cggcacggcc aatggggtac ccccaatggc caccaagaaa gctgagcctg ctgacctgca
     6721 gggcatgctg cttaagaacc ctgaggcacc taacagccat gaggccagcg aggatgacgt
     6781 ggacgcgtcg gagcccatgt acggctgtga catctgcggg gcggcctaca ccatggaggt
     6841 gctgctgcag aatcaccggc tgcgggacca caatatccgg ccgggcgagg atgatggctc
     6901 acgcaagaag gctgagttta tcaagggcag tcacaagtgc aacgtttgtt cacggacttt
     6961 cttctcggag aacgggctac gggagcacct gcagacgcac cggggccctg ccaagcacta
     7021 catgtgtccc atctgtggtg agcgcttccc ttcgctgctg acgctcaccg aacacaaggt
     7081 gacccacagc aagagcctgg acacgggcac ctgtcgcatc tgcaagatgc ccctgcagag
     7141 cgaggaggag tttattgagc actgccagat gcaccctgac ctgcgcaact cactcacggg
     7201 cttccgctgt gtggtctgca tgcagacagt cacttccacg cttgagctca agatccatgg
     7261 caccttccac atgcagaagc tggcgggcag ctcagcggcg tcctccccca atggccaggg
     7321 gctgcagaag ctctacaagt gcgccctgtg cctcaaggag ttccgcagca agcaggacct
     7381 ggtgaagctt gacgtcaatg ggctgcccta cggcctctgc gccggctgca tggcccgcag
     7441 cgccaacgga caggtgggtg gcctggcccc gcccgagccc gccgaccggc cctgtgccgg
     7501 cctccgttgc cccgagtgca gtgtcaagtt tgagagtgcc gaagacctgg agagccacat
     7561 gcaggtggac caccgtgacc tcacgccgga gaccagtggg ccccggaaag gcacccagac
     7621 atcgccagtg ccccggaaaa agacatacca gtgcatcaag tgccagatga ccttcgagaa
     7681 cgagagagag atccaaatcc acgttgccaa ccacatgatt gaggaaggca tcaaccacga
     7741 gtgtaagctg tgcaaccaga tgttcgactc cccggccaag ctcctctgtc acctcattga
     7801 gcacagcttc gagggcatgg gcggcacctt caaatgcccc gtgtgtttca cagtcttcgt
     7861 ccaggccaac aagttgcagc agcacatctt tgccgtgcac gggcaggagg acaagatcta
     7921 cgactgctca cagtgccctc agaagttctt cttccagacc gagctgcaga accacacgat
     7981 gagccagcac gcacagttgc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     8041 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag
     8101 tatttcgatt tcttggcttt atatatcttg tggaaaggac gagcctactc aagcgcaagc
     8161 gaatgcacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8221 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8281 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8341 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8401 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8461 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8521 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8581 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8641 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8701 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8761 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     8821 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     8881 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8941 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9001 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9061 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     9121 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9181 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9241 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9301 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9361 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9421 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     9481 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9541 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9601 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     9661 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9721 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9781 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9841 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9901 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9961 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10021 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10081 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    10141 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10201 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10261 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10321 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10381 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10441 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10501 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10561 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10621 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10681 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10741 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10801 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    10861 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    10921 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10981 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11041 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11101 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11161 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11221 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11281 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11341 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11401 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11461 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11521 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11581 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11641 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11701 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11761 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    11821 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    11881 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    11941 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12001 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12061 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12121 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12181 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12241 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    12301 gggaacaaaa gctggagctg caagc
//
