LOCUS       sequence-404332-       12439 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1349
FEATURES             Location/Qualifiers
     source          1..12439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8296..8884
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8349..8369)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8767..8778)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8956..9189
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9267..9388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9304..9323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9358..9377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9428..9563
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9583..9602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9584..9602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9612..9629)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9612..9628)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9621..9643)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9770..10225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9857..9876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10067..10088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10251..10355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10356..10424,10425..11216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10574..10593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11387..11975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11876..11895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12129..12146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12263..12284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12299..12304,12305..12322,12323..12329)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12337..12353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12342..12364
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12361..12377
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12361..12377
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12396..12416
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12398..12416
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gacaccgacc tgttcatgga
     4141 atgtgaggag gaggagttgg agccatggca gaaaatcagt gatgtcattg aggactctgt
     4201 agttgaagat tataattcag tggataaaac taccacagct ggcaatcctt tggtccagca
     4261 aggtggacag ccactcatcc tgacccagaa tccagcccca ggtctgggca caatggttac
     4321 tcaaccagta ttgaggcctg ttcaggtcat gcagaatgcc aatcatgtga ctagttcccc
     4381 tgtggcctca caaccaatat ttatcactac gcagggattt cctgtaagga atgtccggcc
     4441 tgtacaaaat gcaatgaatc aggttgggat tgtgctgaac gtacagcaag gccaaacggt
     4501 tagaccaatt acactagttc cagccccagg tacccagttt gttaagccga cagttggagt
     4561 tccacaagtg ttctcccaga tgacccctgt gaggccaggc tccacaatgc ctgtgaggcc
     4621 caccaccaac accttcacca ccgtcatccc ggccactctt accattcgaa gcaccgtccc
     4681 acagtcccag tcccagcaga ccaagtccac tcccagcact tctaccactc ccactgccac
     4741 acagccaacc tcactggggc aactagctgt tcagtctcca ggccagtcaa accagaccac
     4801 gaatcccaag ctagctccct ccttcccctc tccacctgca gtgagcattg ccagctttgt
     4861 cactgtgaag cgacctggtg ttacaggcga aaatagcaat gaagtggcca aattggtgaa
     4921 tacccttaac accatccctt ccctgggcca gagtcctggg ccagtggtgg tgtccaacaa
     4981 cagctctgct catggctctc aaagaaccag cggacctgag tcttcaatga aagtgacctc
     5041 ttccatccca gtatttgacc tccaggatgg tggacggaaa atatgtccac gatgtaatgc
     5101 tcaatttcgt gttactgaag ctttgagagg tcacatgtgt tactgttgcc cagaaatggt
     5161 tgaataccag aagaaaggaa agtccctgga ttcagaaccc agtgtcccat cagcagcaaa
     5221 gcccccatcc cctgagaaaa cagctcctgt tgcttccaca ccctcttcta cacctattcc
     5281 tgctctgtca ccgcctacca aagtaccaga accaaatgag aacgtgggcg atgccgtcca
     5341 gaccaaactc attatgcttg tagatgactt ctactatgga cgggatggtg gcaaagtagc
     5401 ccagctcaca aatttcccta aggtcgccac atctttccga tgcccacatt gtaccaaaag
     5461 gctaaaaaac aatattcgat tcatgaacca tatgaaacac cacgtagaac tcgatcagca
     5521 gaacggtgag gtagatggtc acactatctg ccagcactgt taccgccagt tttccactcc
     5581 cttccagctt cagtgccact tggaaaatgt tcatagtccc tatgaatcta ctaccaagtg
     5641 caagatctgt gaatgggcgt ttgaaagtga gccactattt ctccagcata tgaaggatac
     5701 tcataagcct ggagagatgc cttatgtttg ccaggtgtgt caatatcgct cctcactcta
     5761 ctctgaggta gatgtccatt ttcggatgat ccatgaggat acccggcatc tgctctgccc
     5821 ttattgcctg aaggtcttca aaaatggcaa tgcattccaa cagcattaca tgaggcacca
     5881 gaagagaaat gtttatcact gcaacaaatg ccggctgcag tttctctttg ccaaggacaa
     5941 aattgaacac aagcttcaac accataaaac cttccgtaaa cccaagcagc tggagggctt
     6001 gaaaccaggc accaaggtga caatccgggc ttcccgaggg cagccacgaa ctgttcctgt
     6061 atcctctaat gatacacctc ccagcgcctt gcaggaggca gcaccgctga cctcctcaat
     6121 ggaccctctg cctgtcttcc tttatccccc tgtccagcgc agcatccaga agagagctgt
     6181 taggaaaatg agtgtcatgg gccggcagac atgcctggag tgcagcttcg agatcccaga
     6241 cttccctaat catttcccta cttacgtaca ctgctctctg tgtcgctata gcacctgctg
     6301 ttctcgagct tatgccaacc acatgatcaa caatcatgtt ccacggaaga gccccaagta
     6361 tttggctttg tttaaaaatt ctgtgagtgg aatcaagctg gcctgcactt catgtacctt
     6421 tgttacctct gtgggcgatg ctatggccaa gcatttggta ttcaacccct ctcacagatc
     6481 cagcagcatc ctgccacggg gactcacttg gatagctcac tcaaggcatg gccagactcg
     6541 tgaccgagtg catgaccgga acgtgaagaa tatgtaccct cctccttcct tccccactaa
     6601 caaagctgcc actgtgaaat ctgcgggggc caccccagct gagcctgaag agctactaac
     6661 tcccttagcc ccagcactcc catcaccagc ctcaactgca accccaccac caacccccac
     6721 tcacccgcag gctttagccc ttccaccgct ggctacagag ggagccgaat gtctgaatgt
     6781 tgatgatcag gatgaaggga gcccagtcac ccaagaacct gagctagcat caggtggtgg
     6841 tggtagtggt ggagttggca aaaaggagca gctgtctgtg aagaagcttc gagtagtact
     6901 gtttgctcta tgctgcaata cagaacaggc agctgaacac ttccgaaatc cccagcgacg
     6961 tattcgccgt tggcttcgac gtttccaggc ctcccagggg gagaatctag agggcaaata
     7021 tctgagcttt gaggcagaag agaaactggc tgagtgggtg ctaacccagc gcgaacaaca
     7081 gctacctgta aatgaggaga ccttgttcca gaaggccacc aaaataggac gttctttgga
     7141 aggggggttt aagatctcct atgagtgggc tgtgcgtttc atgctgcggc accacctgac
     7201 tccccatgcc cggcgagctg tggcccacac cctacctaag gatgtagcag agaatgcagg
     7261 actcttcatt gattttgtac aacggcagat tcacaaccag gacttaccct tgtctatgat
     7321 tgtggctatt gatgagatct ctttgttcct ggatacagag gtgctgagca gtgatgatcg
     7381 aaaggagaat gccctgcaga cagtgggcac aggggaacct tggtgtgatg tagtcctagc
     7441 cattctggca gatggcactg tccttcccac cctggttttc tacagagggc agatggatca
     7501 gcctgctaac atgccagact ccatattgct agaggcaaag gagagtggct acagtgatga
     7561 cgagatcatg gagctgtggt caactcgagt gtggcagaag cacacagctt gccagcgcag
     7621 caaaggcatg cttgtgatgg actgtcatcg cactcacttg tcagaagagg tactggctat
     7681 gcttagtgcc tctagcactt tgcctgcagt ggtcccagca ggctgtagct ccaaaattca
     7741 gccattagat gtatgcatca aaagaactgt caagaacttc ctgcataaaa aatggaagga
     7801 acaggctcgg gaaatggcag atactgcatg tgattctgat gtcctgcttc agctggtgct
     7861 tgtctggctg ggtgaagtgc taggtgtcat tggggactgt ccagagctag ttcagcgctc
     7921 cttcctggtg gctagtgttc tgcctggccc cgatggcaac attaactcac ctacaagaaa
     7981 tgctgacatg caggaggagc taattgcctc cctagaggag caactgaagc tgagtgggga
     8041 acattctgag tcttccactc cacgacccag atcatctcct gaagagacaa ttgagcctga
     8101 aagtcttcac cagctctttg agggtgaaag tgagaccgag tctttctatg gctttgaaga
     8161 agctgaccta gatctgatgg agatttagta atgaactagt ccgtaacttg aaagtatttc
     8221 gatttcttgg ctttatatat cttgtggaaa ggacgattag cgttgacata cgtattcgta
     8281 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8341 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8401 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8461 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8521 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8581 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8641 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8701 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8761 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8821 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8881 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8941 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     9001 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9061 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9121 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9181 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     9241 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     9301 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9361 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     9421 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9481 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9541 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     9601 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     9661 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     9721 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     9781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9961 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    10021 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    10081 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    10141 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    10201 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
    10261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10321 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10381 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    10441 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    10501 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    10561 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    10621 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10681 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10741 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10801 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10861 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10921 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10981 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    11041 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    11101 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    11161 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    11221 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    11281 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    11341 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    11401 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    11461 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    11521 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    11581 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    11641 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11701 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11761 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11821 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11881 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11941 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    12001 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    12061 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    12121 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    12181 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    12241 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    12301 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    12361 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    12421 aaaagctgga gctgcaagc
//
