LOCUS       sequence-404571-       10942 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10942)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1020
FEATURES             Location/Qualifiers
     source          1..10942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6799..7387
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6852..6872)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7270..7281)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7459..7692
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7770..7891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7807..7826)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7861..7880
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7931..8066
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8086..8105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8087..8105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8115..8132)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8115..8131)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8124..8146)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8273..8728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8360..8379)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8570..8591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8754..8858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8859..8927,8928..9719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9077..9096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9890..10478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10379..10398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10632..10649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10766..10787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10802..10807,10808..10825,10826..10832)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10840..10856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10845..10867
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10864..10880
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10864..10880
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10899..10919
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10901..10919
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac ctcccggcgc tgctccccgc
     4141 cccgactgcg cgcggagggc aacatggcgg cggccccggc ccgctccgcc gagccccagc
     4201 gccgctcggc gcgagccccg cgcgccgccg cctgctactg gtgcggggcc ctgaagatgg
     4261 cgggcccggg gcgcggcccg gggaggcctc cgggccaagc ccgccgcccg ccgaggacga
     4321 cagcgacggc gactctttct tggtgctgct ggaagtgccg cacggcggcg ctgccgccga
     4381 ggctgccgga tcacaggagg ccgagcctgg ctcccgtgtc aacctggcga gccgccccga
     4441 gcagggcccc agcggcccgg ccgccccccc cggccctggc gtagccccgg cgggcgccgt
     4501 caccatcagc agccaggacc tgctggtgcg tctcgaccgc ggcgtcctcg cgctgtctgc
     4561 gccgcccggc cccgcaaccg cgggcgccgc cgctccccgc cgcgcgcccc aggcctccgg
     4621 ccccagcacg cccggctacc gctgccccga gccgcagtgc gcgctggcct tcgccaagaa
     4681 gcaccagctc aaggtgcacc tgctcacgca cggcggcggt cagggccggc ggcccttcaa
     4741 gtgcccactg gagggctgtg gttgggcctt cacaacgtcc tacaagctca agcggcacct
     4801 gcagtcgcac gacaagctgc ggcccttcgg ctgtccagtg ggcggctgtg gcaagaagtt
     4861 cactacggtc tataacctca aggcgcacat gaagggccac gagcaggaga gcctgttcaa
     4921 gtgcgaggtg tgcgccgagc gcttccccac gcacgccaag ctcagctccc accagcgcag
     4981 ccacttcgag cccgagcgcc cttacaagtg tgactttccc ggctgtgaga agacatttat
     5041 cacagtgagt gccctgtttt cccataaccg agcccacttc agggaacaag agctcttttc
     5101 ctgctccttt cctgggtgca gcaagcagta tgataaagcc tgtcggctga aaattcacct
     5161 gcggagccat acaggtgaaa gaccatttat ttgtgactct gacagctgtg gctggacctt
     5221 caccagcatg tccaaacttc taaggcacag aaggaaacat gacgatgacc ggaggtttac
     5281 ctgccctgtc gagggctgtg ggaaatcatt caccagagca gagcatctga aaggccacag
     5341 cataacccac ctaggcacaa agccgttcga gtgtcctgtg gaaggatgtt gcgcgaggtt
     5401 ctccgctcgt agcagtctgt acattcactc taagaaacac gtgcaggatg tgggtgctcc
     5461 gaaaagccgt tgcccagttt ctacctgcaa cagactcttc acctccaagc acagcatgaa
     5521 ggcgcacatg gtcagacagc acagccggcg ccaagatctc ttacctcagc tagaagctcc
     5581 gagttctctt actcccagca gtgaactcag cagcccaggc caaagtgagc tcactaacat
     5641 ggatcttgct gcactcttct ctgacacacc tgccaatgct agtggttctg caggtgggtc
     5701 ggatgaggct ctgaactccg gaatcctgac tattgacgtc acttctgtga gctcctctct
     5761 gggagggaac ctccctgcta ataatagctc cctagggccg atggaacccc tggtcctggt
     5821 ggcccacagt gatattcccc caagcctgga cagccctctg gttctcggga cagcagccac
     5881 ggttctgcag cagggcagct tcagtgtgga tgacgtgcag actgtgagtg caggagcatt
     5941 aggctgtctg gtggctctgc ccatgaagaa cttgagtgac gacccactgg ctttgacctc
     6001 caatagtaac ttagcagcac atatcaccac accgacctct tcgagcaccc cccgagaaaa
     6061 tgccagtgtc ccggaactgc tggctccaat caaggtggag ccggactcgc cttctcgccc
     6121 aggagcagtt gggcagcagg aaggaagcca tgggctgccc cagtccacgt tgcccagtcc
     6181 agcagagcag cacggtgccc aggacacaga gctcagtgca ggcactggca acttctattt
     6241 ggaaagtggg ggctcagcaa gaactgatta ccgagccatt caactagcca aggaaaaaaa
     6301 gcagagagga gcggggagca atgcaggagc ctcacagtct actcagagaa aaataaaaga
     6361 aggcaaaatg agtcctcccc atttccatgc aagccagaac agttggttgt gtgggagcct
     6421 cgtggtgccc agcggaggac ggccaggacc agctccagca gctggggtgc agtgcggggc
     6481 gcagggcgtc caggtccagc tggtgcagga tgacccctcc ggcgaaggtg tcctgccctc
     6541 ggcccgcggc ccagccacct tcctcccctt cctcactgtg gacctgcccg tctacgtcct
     6601 ccaggaggtg ctcccctcat ctggaggccc tgctggaccg gaggccaccc agttcccagg
     6661 aagcactatc aacctgcagg atctgcagta gtaatgaact agtccgtaac ttgaaagtat
     6721 ttcgatttct tggctttata tatcttgtgg aaaggacgaa tcccataact ggcgtttgta
     6781 gagacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6841 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6901 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6961 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7021 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7081 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7141 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7201 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7261 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7321 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7381 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7441 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7501 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7561 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7621 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7681 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     7741 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7801 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7861 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7921 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7981 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8041 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     8101 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8161 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8221 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     8281 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8341 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8401 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8461 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8581 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8641 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8701 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8761 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8821 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8881 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8941 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9001 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9061 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9121 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9181 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9241 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9301 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9361 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9421 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9481 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9541 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9601 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9661 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9721 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10501 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10561 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10621 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10681 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10741 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10801 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    10861 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    10921 aacaaaagct ggagctgcaa gc
//
